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Protein

LIM domain-containing protein ajuba

Gene

Ajuba

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.5 Publications

Miscellaneous

'Ajuba' means 'curiosity' in Urdu, an Indian dialect.

GO - Molecular functioni

  • actin filament binding Source: MGI
  • alpha-catenin binding Source: MGI
  • chromatin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  • cell cycle Source: UniProtKB-KW
  • cellular protein localization Source: MGI
  • cytoskeleton organization Source: GO_Central
  • focal adhesion assembly Source: MGI
  • gene silencing by miRNA Source: MGI
  • glycerophospholipid biosynthetic process Source: MGI
  • hippo signaling Source: GO_Central
  • lamellipodium assembly Source: MGI
  • negative regulation of hippo signaling Source: UniProtKB
  • negative regulation of kinase activity Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of cellular biosynthetic process Source: MGI
  • positive regulation of gene silencing by miRNA Source: UniProtKB
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of kinase activity Source: MGI
  • positive regulation of MAP kinase activity Source: MGI
  • positive regulation of protein complex assembly Source: MGI
  • regulation of cell migration Source: MGI
  • regulation of cellular response to hypoxia Source: MGI
  • regulation of GTPase activity Source: MGI
  • response to hypoxia Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • wound healing, spreading of epidermal cells Source: MGI

Keywordsi

Molecular functionRepressor
Biological processCell adhesion, Cell cycle, RNA-mediated gene silencing, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition

Names & Taxonomyi

Protein namesi
Recommended name:
LIM domain-containing protein ajuba
Gene namesi
Name:Ajuba
Synonyms:Jub
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1341886 Ajuba

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003126261 – 547LIM domain-containing protein ajubaAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89PhosphoserineBy similarity1
Modified residuei129PhosphoserineBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by LATS2 during mitosis. Phosphorylated by AURKA (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91XC0
PaxDbiQ91XC0
PeptideAtlasiQ91XC0
PRIDEiQ91XC0

PTM databases

iPTMnetiQ91XC0
PhosphoSitePlusiQ91XC0

Expressioni

Tissue specificityi

Expressed in skin, brain and genitourinary organs.1 Publication

Developmental stagei

Detected in all embryonic germ layers, in the extraembryonic yolk sac blood islands and in the fetal components of the developing placenta. As development progressed, expression is dramatically restricted.1 Publication

Gene expression databases

BgeeiENSMUSG00000022178
CleanExiMM_JUB
GenevisibleiQ91XC0 MM

Interactioni

Subunit structurei

Interacts with SLC1A2. Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3 (By similarity). Interacts with GRB2 and PIP5K1B. Interacts with HDAC1, HDAC2 and HDAC3. Interacts with EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with VHL (By similarity). Interacts (via LIM domains) with SNAI1 (via SNAG domain), SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin filament binding Source: MGI
  • alpha-catenin binding Source: MGI

Protein-protein interaction databases

BioGridi200870, 1 interactor
DIPiDIP-38451N
IntActiQ91XC0, 8 interactors
STRINGi10090.ENSMUSP00000056977

Structurei

3D structure databases

ProteinModelPortaliQ91XC0
SMRiQ91XC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini345 – 406LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST62
Domaini410 – 470LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST61
Domaini471 – 539LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 344PreLIMAdd BLAST344

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi289 – 297Nuclear localization signalSequence analysis9

Domaini

LIM region interacts with CTNNA1. The preLIM region binds directly actin filaments (By similarity).By similarity
LIM-2 and LIM-3 domains mediate the interaction with the N-terminal region of AURKA. The association between LATS2 and AJUBA required the second LIM domain of AJUBA (By similarity).By similarity

Sequence similaritiesi

Belongs to the zyxin/ajuba family.Curated

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1701 Eukaryota
ENOG410Y3GP LUCA
GeneTreeiENSGT00760000119039
HOGENOMiHOG000113139
HOVERGENiHBG095660
InParanoidiQ91XC0
KOiK16682
OMAiRQPPTNY
OrthoDBiEOG091G057U
PhylomeDBiQ91XC0
TreeFamiTF320310

Family and domain databases

InterProiView protein in InterPro
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00412 LIM, 3 hits
SMARTiView protein in SMART
SM00132 LIM, 3 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 2 hits
PS50023 LIM_DOMAIN_2, 3 hits

Sequencei

Sequence statusi: Complete.

Q91XC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERLGEKASR LLEKLRLSDS GSAKFGRRKG EASRSGSDGT PGAGKGRLSG
60 70 80 90 100
LGGPRKSGHR GANGGPGDEP LEPAREQGPL DAERNARGSF EAQRFEGSFP
110 120 130 140 150
GGPPPTRALP LPLSSPPDFR LETTAPALSP RSSFASSSAS DASKPSSPRG
160 170 180 190 200
SLLLDGAGAS GAGGSRPCSN RTSGISMGYD QRHGSPLPAG PCLFGLPLTT
210 220 230 240 250
APAGYPGGAP SAYPELHAAL DRLCAHRSVG FGCQESRHSY PPALGSPGAL
260 270 280 290 300
TGAVVGTAGP LERRGAQPGR HSVTGYGDCA AGARYQDELT ALLRLTVATG
310 320 330 340 350
GREAGARGEP SGIEPSGLEE SPGPFVPEAS RSRIREPEAR EDYFGTCIKC
360 370 380 390 400
NKGIYGQSNA CQALDSLYHT QCFVCCSCGR TLRCKAFYSV NGSVYCEEDY
410 420 430 440 450
LFSGFQEAAE KCCVCGHLIL EKILQAMGKS YHPGCFRCIV CNKCLDGVPF
460 470 480 490 500
TVDFSNQVYC VTDYHKNYAP KCAACGQPIL PSEGCEDIVR VISMDRDYHF
510 520 530 540
ECYHCEDCRM QLSDEEGCCC FPLDGHLLCH GCHMQRLSAR QPSTNYI
Length:547
Mass (Da):57,920
Last modified:December 1, 2001 - v1
Checksum:iC326772AC1C155B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti534M → I in AAB38287 (PubMed:10330178).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79776 mRNA Translation: AAB38287.1
BC011035 mRNA Translation: AAH11035.1
CCDSiCCDS27093.1
RefSeqiNP_034720.2, NM_010590.5
UniGeneiMm.100253

Genome annotation databases

EnsembliENSMUST00000054487; ENSMUSP00000056977; ENSMUSG00000022178
GeneIDi16475
KEGGimmu:16475
UCSCiuc007twj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiAJUBA_MOUSE
AccessioniPrimary (citable) accession number: Q91XC0
Secondary accession number(s): P97472
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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