Reviewed, UniProtKB/Swiss-Prot Q91X88 (PMGT1_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
      Short name=POMGnT1
    EC=2.4.1.-

Gene names

Name:

Pomgnt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in O-mannosyl glycosylation. May be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins. Is specific for alpha linked terminal mannose By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + Man-R = N-acetyl-D-glucosamine-beta-1,2-Man-R + UDP.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Developmental stage	Broadly expressed in late embryonic and early postnatal cerebellar neurons, including premigratory granule neurons of the external granule cell layer. Expression is maintained in neurons of the internal granule cell layer after migration is complete. Expressed in Purkinje cells throughout development. Expressed in Bergmann glial scaffolds used by granule cells during early posnatal radial migration. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro protein amino acid O-linked glycosylation Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91X88-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91X88-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
     23-40: LTWKYKLTNQRALRRFCQ → MEGTKEVKDSNGKIQDHG

Isoform 3 (identifier: Q91X88-3)

The sequence of this isoform differs from the canonical sequence as follows:
     218-250: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1		PRO_0000191391
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Signal-anchor for type II membrane protein Potential
	Topological domain	59 – 660	602	Lumenal Potential
	Compositional bias	263 – 267	5	Poly-Arg
Amino acid modifications
	Cross-link	537		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	538		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Natural variations
	Alternative sequence	1 – 22	22	Missing in isoform 2.		VSP_014972
	Alternative sequence	23 – 40	18	LTWKY…RRFCQ → MEGTKEVKDSNGKIQDHG in isoform 2.		VSP_014973
	Alternative sequence	218 – 250	33	Missing in isoform 3.		VSP_014974
Experimental info
	Sequence conflict	424	1	A → S in BAB29863. Ref.2
	Sequence conflict	557	1	H → P in BAB22251. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 4DE09765C0F3EF7C	FASTA	660	75,110
	10 20 30 40 50 60 MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT VIVNIKLILD 70 80 90 100 110 120 TRRAISEANE DPEPEQDYDE ALGRLESPRR RGSSPRRVLD VEVYSSRSKV YVAVDGTTVL 130 140 150 160 170 180 EDEAREQGRG IHVIVLNQAT GHVMAKRVFD TYSPHEDEAM VLFLNMVAPG RVLICTVKDE 190 200 210 220 230 240 GSFHLKDTAK ALLRSLGSQA GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL 250 260 270 280 290 300 KTDVPLSSAE EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL 310 320 330 340 350 360 NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL FGLRGIQHTP 370 380 390 400 410 420 ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV DFFSFLSQSI HLLEEDDSLY 430 440 450 460 470 480 CISAWNDQGY EHTAEDPALL YRVETMPGLG WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR 490 500 510 520 530 540 MPEQRRGREC IIPDVSRSYH FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA 550 560 570 580 590 600 YEVEIHRLLS EAEVLDHSKD PCEDSFLPDT EGHTYVAFIR METDDDFATW TQLAKCLHIW 610 620 630 640 650 660 DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSVKKPPSVT PIFLEPPPKE EGAPGAAEQT
	Isoform 2. Checksum: A8656C7EA33D2767	FASTA	638	72,068
	10 20 30 40 50 60 MEGTKEVKDS NGKIQDHGTG AVLFLLVTVI VNIKLILDTR RAISEANEDP EPEQDYDEAL 70 80 90 100 110 120 GRLESPRRRG SSPRRVLDVE VYSSRSKVYV AVDGTTVLED EAREQGRGIH VIVLNQATGH 130 140 150 160 170 180 VMAKRVFDTY SPHEDEAMVL FLNMVAPGRV LICTVKDEGS FHLKDTAKAL LRSLGSQAGP 190 200 210 220 230 240 ALGWRDTWAF VGRKGGPVLG EKHSKSPALS SWGDPVLLKT DVPLSSAEEA ECHWADTELN 250 260 270 280 290 300 RRRRRFCSKV EGYGSVCSCK DPTPIEFSPD PLPDNKVLNV PVAVIAGNRP NYLYRMLRSL 310 320 330 340 350 360 LSAQGVSPQM ITVFIDGYYE EPMDVVALFG LRGIQHTPIS IKNARVSQHY KASLTATFNL 370 380 390 400 410 420 FPEAKFAVVL EEDLDIAVDF FSFLSQSIHL LEEDDSLYCI SAWNDQGYEH TAEDPALLYR 430 440 450 460 470 480 VETMPGLGWV LRKSLYKEEL EPKWPTPEKL WDWDMWMRMP EQRRGRECII PDVSRSYHFG 490 500 510 520 530 540 IVGLNMNGYF HEAYFKKHKF NTVPGVQLRN VDSLKKEAYE VEIHRLLSEA EVLDHSKDPC 550 560 570 580 590 600 EDSFLPDTEG HTYVAFIRME TDDDFATWTQ LAKCLHIWDL DVRGNHRGLW RLFRKKNHFL 610 620 630 VVGVPASPYS VKKPPSVTPI FLEPPPKEEG APGAAEQT
	Isoform 3. Checksum: 334E4F56BC5D342B	FASTA	627	71,725
	10 20 30 40 50 60 MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT VIVNIKLILD 70 80 90 100 110 120 TRRAISEANE DPEPEQDYDE ALGRLESPRR RGSSPRRVLD VEVYSSRSKV YVAVDGTTVL 130 140 150 160 170 180 EDEAREQGRG IHVIVLNQAT GHVMAKRVFD TYSPHEDEAM VLFLNMVAPG RVLICTVKDE 190 200 210 220 230 240 GSFHLKDTAK ALLRSLGSQA GPALGWRDTW AFVGRKGEAE CHWADTELNR RRRRFCSKVE 250 260 270 280 290 300 GYGSVCSCKD PTPIEFSPDP LPDNKVLNVP VAVIAGNRPN YLYRMLRSLL SAQGVSPQMI 310 320 330 340 350 360 TVFIDGYYEE PMDVVALFGL RGIQHTPISI KNARVSQHYK ASLTATFNLF PEAKFAVVLE 370 380 390 400 410 420 EDLDIAVDFF SFLSQSIHLL EEDDSLYCIS AWNDQGYEHT AEDPALLYRV ETMPGLGWVL 430 440 450 460 470 480 RKSLYKEELE PKWPTPEKLW DWDMWMRMPE QRRGRECIIP DVSRSYHFGI VGLNMNGYFH 490 500 510 520 530 540 EAYFKKHKFN TVPGVQLRNV DSLKKEAYEV EIHRLLSEAE VLDHSKDPCE DSFLPDTEGH 550 560 570 580 590 600 TYVAFIRMET DDDFATWTQL AKCLHIWDLD VRGNHRGLWR LFRKKNHFLV VGVPASPYSV 610 620 KKPPSVTPIF LEPPPKEEGA PGAAEQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Minowa M.T., Yoshida A., Endo T., Takeuchi M. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Strain: 129/SvJ.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Kidney and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Salivary gland.
[4]	"Expression of dystroglycan, fukutin and POMGnT1 during mouse cerebellar development." Henion T.R., Qu Q., Smith F.I. Brain Res. Mol. Brain Res. 112:177-181(2003) [PubMed: 12670716] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

POMGnT1

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB053220 mRNA. Translation: BAC55021.1. AB053221 Genomic DNA. Translation: BAC55022.1. AK002638 mRNA. Translation: BAB22251.1. AK019640 mRNA. Translation: BAB31822.1. AK015478 mRNA. Translation: BAB29863.1. BC011201 mRNA. Translation: AAH11201.1.
IPI	IPI00121081. IPI00163020. IPI00271974.
RefSeq	NP_080927.1.
UniGene	Mm.196045 Mm.474251
3D structure databases
HSSP	HSSP built from PDB template 1FO8 based on UniProtKB P27115.
ModBase	Search...
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
PTM databases
PhosphoSite	Q91X88.
Genome annotation databases
Ensembl	ENSMUSG00000028700. Mus musculus. [Contig view]
GeneID	68273.
KEGG	mmu:68273.
Organism-specific databases
MGI	MGI:1915523. Pomgnt1.
Phylogenomic databases
HOGENOM	Q91X88.
HOVERGEN	Q91X88.
OMA	Q91X88. AYEVEIH.
Gene expression databases
ArrayExpress	Q91X88.
Bgee	Q91X88.
GermOnline	ENSMUSG00000028700. Mus musculus.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	326888.
SOURCE	Search...

Entry information

Entry name

PMGT1_MOUSE

Accession

Primary (citable) accession number: Q91X88
Secondary accession number(s): Q9D2H7, Q9D5D3, Q9DCN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)