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Q91X88

- PMGT1_MOUSE

UniProt

Q91X88 - PMGT1_MOUSE

Protein

Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1

Gene

Pomgnt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Participates in O-mannosyl glycosylation. May be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins. Is specific for alpha linked terminal mannose By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.

    Cofactori

    Manganese.By similarity

    Pathwayi

    GO - Molecular functioni

    1. acetylglucosaminyltransferase activity Source: InterPro

    GO - Biological processi

    1. protein O-linked glycosylation Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT13. Glycosyltransferase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (EC:2.4.1.-)
    Short name:
    POMGnT1
    Gene namesi
    Name:Pomgnt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1915523. Pomgnt1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 660660Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1PRO_0000191391Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki537 – 537Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki538 – 538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PRIDEiQ91X88.

    PTM databases

    PhosphoSiteiQ91X88.

    Expressioni

    Developmental stagei

    Broadly expressed in late embryonic and early postnatal cerebellar neurons, including premigratory granule neurons of the external granule cell layer. Expression is maintained in neurons of the internal granule cell layer after migration is complete. Expressed in Purkinje cells throughout development. Expressed in Bergmann glial scaffolds used by granule cells during early posnatal radial migration.1 Publication

    Gene expression databases

    BgeeiQ91X88.
    GenevestigatoriQ91X88.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91X88.
    SMRiQ91X88. Positions 128-217, 302-542.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3737CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini59 – 660602LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei38 – 5821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi263 – 2675Poly-Arg

    Sequence similaritiesi

    Belongs to the glycosyltransferase 13 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148227.
    GeneTreeiENSGT00530000063632.
    HOGENOMiHOG000231121.
    HOVERGENiHBG055279.
    InParanoidiA2A8F8.
    KOiK09666.
    OMAiNHFLVVG.
    OrthoDBiEOG786H2N.
    PhylomeDBiQ91X88.
    TreeFamiTF320555.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10468. PTHR10468. 1 hit.
    PfamiPF03071. GNT-I. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q91X88-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT    50
    VIVNIKLILD TRRAISEANE DPEPEQDYDE ALGRLESPRR RGSSPRRVLD 100
    VEVYSSRSKV YVAVDGTTVL EDEAREQGRG IHVIVLNQAT GHVMAKRVFD 150
    TYSPHEDEAM VLFLNMVAPG RVLICTVKDE GSFHLKDTAK ALLRSLGSQA 200
    GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL KTDVPLSSAE 250
    EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL 300
    NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL 350
    FGLRGIQHTP ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV 400
    DFFSFLSQSI HLLEEDDSLY CISAWNDQGY EHTAEDPALL YRVETMPGLG 450
    WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR MPEQRRGREC IIPDVSRSYH 500
    FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA YEVEIHRLLS 550
    EAEVLDHSKD PCEDSFLPDT EGHTYVAFIR METDDDFATW TQLAKCLHIW 600
    DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSVKKPPSVT PIFLEPPPKE 650
    EGAPGAAEQT 660
    Length:660
    Mass (Da):75,110
    Last modified:December 1, 2001 - v1
    Checksum:i4DE09765C0F3EF7C
    GO
    Isoform 2 (identifier: Q91X88-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-22: Missing.
         23-40: LTWKYKLTNQRALRRFCQ → MEGTKEVKDSNGKIQDHG

    Show »
    Length:638
    Mass (Da):72,068
    Checksum:iA8656C7EA33D2767
    GO
    Isoform 3 (identifier: Q91X88-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         218-250: Missing.

    Show »
    Length:627
    Mass (Da):71,725
    Checksum:i334E4F56BC5D342B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti424 – 4241A → S in BAB29863. (PubMed:16141072)Curated
    Sequence conflicti557 – 5571H → P in BAB22251. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2222Missing in isoform 2. 1 PublicationVSP_014972Add
    BLAST
    Alternative sequencei23 – 4018LTWKY…RRFCQ → MEGTKEVKDSNGKIQDHG in isoform 2. 1 PublicationVSP_014973Add
    BLAST
    Alternative sequencei218 – 25033Missing in isoform 3. 1 PublicationVSP_014974Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB053220 mRNA. Translation: BAC55021.1.
    AB053221 Genomic DNA. Translation: BAC55022.1.
    AK002638 mRNA. Translation: BAB22251.1.
    AK019640 mRNA. Translation: BAB31822.1.
    AK015478 mRNA. Translation: BAB29863.1.
    AL611947 Genomic DNA. Translation: CAM20673.1.
    BC011201 mRNA. Translation: AAH11201.1.
    CCDSiCCDS18506.1. [Q91X88-1]
    CCDS18507.1. [Q91X88-3]
    CCDS71450.1. [Q91X88-2]
    RefSeqiNP_001277587.1. NM_001290658.1. [Q91X88-2]
    NP_080927.1. NM_026651.2. [Q91X88-1]
    NP_084062.2. NM_029786.2. [Q91X88-3]
    UniGeneiMm.196045.

    Genome annotation databases

    EnsembliENSMUST00000106494; ENSMUSP00000102103; ENSMUSG00000028700. [Q91X88-2]
    ENSMUST00000106496; ENSMUSP00000102105; ENSMUSG00000028700. [Q91X88-3]
    ENSMUST00000106498; ENSMUSP00000102107; ENSMUSG00000028700. [Q91X88-1]
    ENSMUST00000120083; ENSMUSP00000112751; ENSMUSG00000028700. [Q91X88-1]
    ENSMUST00000121052; ENSMUSP00000112911; ENSMUSG00000028700. [Q91X88-1]
    GeneIDi68273.
    KEGGimmu:68273.
    UCSCiuc008ugh.2. mouse. [Q91X88-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    POMGnT1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB053220 mRNA. Translation: BAC55021.1 .
    AB053221 Genomic DNA. Translation: BAC55022.1 .
    AK002638 mRNA. Translation: BAB22251.1 .
    AK019640 mRNA. Translation: BAB31822.1 .
    AK015478 mRNA. Translation: BAB29863.1 .
    AL611947 Genomic DNA. Translation: CAM20673.1 .
    BC011201 mRNA. Translation: AAH11201.1 .
    CCDSi CCDS18506.1. [Q91X88-1 ]
    CCDS18507.1. [Q91X88-3 ]
    CCDS71450.1. [Q91X88-2 ]
    RefSeqi NP_001277587.1. NM_001290658.1. [Q91X88-2 ]
    NP_080927.1. NM_026651.2. [Q91X88-1 ]
    NP_084062.2. NM_029786.2. [Q91X88-3 ]
    UniGenei Mm.196045.

    3D structure databases

    ProteinModelPortali Q91X88.
    SMRi Q91X88. Positions 128-217, 302-542.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT13. Glycosyltransferase Family 13.

    PTM databases

    PhosphoSitei Q91X88.

    Proteomic databases

    PRIDEi Q91X88.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000106494 ; ENSMUSP00000102103 ; ENSMUSG00000028700 . [Q91X88-2 ]
    ENSMUST00000106496 ; ENSMUSP00000102105 ; ENSMUSG00000028700 . [Q91X88-3 ]
    ENSMUST00000106498 ; ENSMUSP00000102107 ; ENSMUSG00000028700 . [Q91X88-1 ]
    ENSMUST00000120083 ; ENSMUSP00000112751 ; ENSMUSG00000028700 . [Q91X88-1 ]
    ENSMUST00000121052 ; ENSMUSP00000112911 ; ENSMUSG00000028700 . [Q91X88-1 ]
    GeneIDi 68273.
    KEGGi mmu:68273.
    UCSCi uc008ugh.2. mouse. [Q91X88-1 ]

    Organism-specific databases

    CTDi 55624.
    MGIi MGI:1915523. Pomgnt1.

    Phylogenomic databases

    eggNOGi NOG148227.
    GeneTreei ENSGT00530000063632.
    HOGENOMi HOG000231121.
    HOVERGENi HBG055279.
    InParanoidi A2A8F8.
    KOi K09666.
    OMAi NHFLVVG.
    OrthoDBi EOG786H2N.
    PhylomeDBi Q91X88.
    TreeFami TF320555.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    ChiTaRSi POMGNT1. mouse.
    NextBioi 326888.
    PROi Q91X88.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91X88.
    Genevestigatori Q91X88.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10468. PTHR10468. 1 hit.
    Pfami PF03071. GNT-I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Minowa M.T., Yoshida A., Endo T., Takeuchi M.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: 129/SvJ.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Kidney and Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Salivary gland.
    5. "Expression of dystroglycan, fukutin and POMGnT1 during mouse cerebellar development."
      Henion T.R., Qu Q., Smith F.I.
      Brain Res. Mol. Brain Res. 112:177-181(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiPMGT1_MOUSE
    AccessioniPrimary (citable) accession number: Q91X88
    Secondary accession number(s): A2A8F8
    , Q9D2H7, Q9D5D3, Q9DCN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3