Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1

Gene

Pomgnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in O-mannosyl glycosylation. May be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins. Is specific for alpha linked terminal mannose.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.By similarity

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein O-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

ReactomeiR-MMU-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (EC:2.4.1.-By similarity)
Short name:
POMGnT1
Gene namesi
Name:Pomgnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915523. Pomgnt1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini59 – 660LumenalSequence analysisAdd BLAST602

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913911 – 660Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91X88.
PeptideAtlasiQ91X88.
PRIDEiQ91X88.

PTM databases

iPTMnetiQ91X88.
PhosphoSitePlusiQ91X88.
SwissPalmiQ91X88.

Expressioni

Tissue specificityi

Expressed at basal body and daughter centriole of photoreceptor cells (at protein level).1 Publication

Developmental stagei

Broadly expressed in late embryonic and early postnatal cerebellar neurons, including premigratory granule neurons of the external granule cell layer. Expression is maintained in neurons of the internal granule cell layer after migration is complete. Expressed in Purkinje cells throughout development. Expressed in Bergmann glial scaffolds used by granule cells during early posnatal radial migration.1 Publication

Gene expression databases

BgeeiENSMUSG00000028700.
GenevisibleiQ91X88. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102107.

Structurei

3D structure databases

ProteinModelPortaliQ91X88.
SMRiQ91X88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi263 – 267Poly-Arg5

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFXX. Eukaryota.
ENOG410YMAS. LUCA.
GeneTreeiENSGT00530000063632.
HOGENOMiHOG000231121.
HOVERGENiHBG055279.
InParanoidiQ91X88.
KOiK09666.
OMAiPYSVKKP.
OrthoDBiEOG091G00JT.
PhylomeDBiQ91X88.
TreeFamiTF320555.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 2 hits.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91X88-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT
60 70 80 90 100
VIVNIKLILD TRRAISEANE DPEPEQDYDE ALGRLESPRR RGSSPRRVLD
110 120 130 140 150
VEVYSSRSKV YVAVDGTTVL EDEAREQGRG IHVIVLNQAT GHVMAKRVFD
160 170 180 190 200
TYSPHEDEAM VLFLNMVAPG RVLICTVKDE GSFHLKDTAK ALLRSLGSQA
210 220 230 240 250
GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL KTDVPLSSAE
260 270 280 290 300
EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL
310 320 330 340 350
NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL
360 370 380 390 400
FGLRGIQHTP ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV
410 420 430 440 450
DFFSFLSQSI HLLEEDDSLY CISAWNDQGY EHTAEDPALL YRVETMPGLG
460 470 480 490 500
WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR MPEQRRGREC IIPDVSRSYH
510 520 530 540 550
FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA YEVEIHRLLS
560 570 580 590 600
EAEVLDHSKD PCEDSFLPDT EGHTYVAFIR METDDDFATW TQLAKCLHIW
610 620 630 640 650
DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSVKKPPSVT PIFLEPPPKE
660
EGAPGAAEQT
Length:660
Mass (Da):75,110
Last modified:December 1, 2001 - v1
Checksum:i4DE09765C0F3EF7C
GO
Isoform 2 (identifier: Q91X88-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
     23-40: LTWKYKLTNQRALRRFCQ → MEGTKEVKDSNGKIQDHG

Show »
Length:638
Mass (Da):72,068
Checksum:iA8656C7EA33D2767
GO
Isoform 3 (identifier: Q91X88-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     218-250: Missing.

Show »
Length:627
Mass (Da):71,725
Checksum:i334E4F56BC5D342B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti424A → S in BAB29863 (PubMed:16141072).Curated1
Sequence conflicti557H → P in BAB22251 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0149721 – 22Missing in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_01497323 – 40LTWKY…RRFCQ → MEGTKEVKDSNGKIQDHG in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_014974218 – 250Missing in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053220 mRNA. Translation: BAC55021.1.
AB053221 Genomic DNA. Translation: BAC55022.1.
AK002638 mRNA. Translation: BAB22251.1.
AK019640 mRNA. Translation: BAB31822.1.
AK015478 mRNA. Translation: BAB29863.1.
AL611947 Genomic DNA. Translation: CAM20673.1.
BC011201 mRNA. Translation: AAH11201.1.
CCDSiCCDS18506.1. [Q91X88-1]
CCDS18507.1. [Q91X88-3]
CCDS71450.1. [Q91X88-2]
RefSeqiNP_001277587.1. NM_001290658.1. [Q91X88-2]
NP_080927.1. NM_026651.2. [Q91X88-1]
NP_084062.2. NM_029786.2. [Q91X88-3]
UniGeneiMm.196045.

Genome annotation databases

EnsembliENSMUST00000106494; ENSMUSP00000102103; ENSMUSG00000028700. [Q91X88-2]
ENSMUST00000106496; ENSMUSP00000102105; ENSMUSG00000028700. [Q91X88-3]
ENSMUST00000106498; ENSMUSP00000102107; ENSMUSG00000028700. [Q91X88-1]
ENSMUST00000120083; ENSMUSP00000112751; ENSMUSG00000028700. [Q91X88-1]
ENSMUST00000121052; ENSMUSP00000112911; ENSMUSG00000028700. [Q91X88-1]
GeneIDi68273.
KEGGimmu:68273.
UCSCiuc008ugh.2. mouse. [Q91X88-1]
uc008ugi.2. mouse. [Q91X88-3]
uc008ugj.1. mouse. [Q91X88-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

POMGnT1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053220 mRNA. Translation: BAC55021.1.
AB053221 Genomic DNA. Translation: BAC55022.1.
AK002638 mRNA. Translation: BAB22251.1.
AK019640 mRNA. Translation: BAB31822.1.
AK015478 mRNA. Translation: BAB29863.1.
AL611947 Genomic DNA. Translation: CAM20673.1.
BC011201 mRNA. Translation: AAH11201.1.
CCDSiCCDS18506.1. [Q91X88-1]
CCDS18507.1. [Q91X88-3]
CCDS71450.1. [Q91X88-2]
RefSeqiNP_001277587.1. NM_001290658.1. [Q91X88-2]
NP_080927.1. NM_026651.2. [Q91X88-1]
NP_084062.2. NM_029786.2. [Q91X88-3]
UniGeneiMm.196045.

3D structure databases

ProteinModelPortaliQ91X88.
SMRiQ91X88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102107.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

PTM databases

iPTMnetiQ91X88.
PhosphoSitePlusiQ91X88.
SwissPalmiQ91X88.

Proteomic databases

PaxDbiQ91X88.
PeptideAtlasiQ91X88.
PRIDEiQ91X88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106494; ENSMUSP00000102103; ENSMUSG00000028700. [Q91X88-2]
ENSMUST00000106496; ENSMUSP00000102105; ENSMUSG00000028700. [Q91X88-3]
ENSMUST00000106498; ENSMUSP00000102107; ENSMUSG00000028700. [Q91X88-1]
ENSMUST00000120083; ENSMUSP00000112751; ENSMUSG00000028700. [Q91X88-1]
ENSMUST00000121052; ENSMUSP00000112911; ENSMUSG00000028700. [Q91X88-1]
GeneIDi68273.
KEGGimmu:68273.
UCSCiuc008ugh.2. mouse. [Q91X88-1]
uc008ugi.2. mouse. [Q91X88-3]
uc008ugj.1. mouse. [Q91X88-2]

Organism-specific databases

CTDi55624.
MGIiMGI:1915523. Pomgnt1.

Phylogenomic databases

eggNOGiENOG410IFXX. Eukaryota.
ENOG410YMAS. LUCA.
GeneTreeiENSGT00530000063632.
HOGENOMiHOG000231121.
HOVERGENiHBG055279.
InParanoidiQ91X88.
KOiK09666.
OMAiPYSVKKP.
OrthoDBiEOG091G00JT.
PhylomeDBiQ91X88.
TreeFamiTF320555.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-5173105. O-linked glycosylation.

Miscellaneous databases

ChiTaRSiPomgnt1. mouse.
PROiQ91X88.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028700.
GenevisibleiQ91X88. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 2 hits.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPMGT1_MOUSE
AccessioniPrimary (citable) accession number: Q91X88
Secondary accession number(s): A2A8F8
, Q9D2H7, Q9D5D3, Q9DCN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.