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Q91X83

- METK1_MOUSE

UniProt

Q91X83 - METK1_MOUSE

Protein

S-adenosylmethionine synthase isoform type-1

Gene

Mat1a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of S-adenosylmethionine from methionine and ATP.By similarity

    Catalytic activityi

    ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.

    Cofactori

    Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.By similarity
    Binds 1 potassium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301SubstrateBy similarity
    Metal bindingi32 – 321MagnesiumBy similarity
    Metal bindingi58 – 581PotassiumBy similarity
    Binding sitei160 – 1601ATPSequence Analysis
    Binding sitei180 – 1801SubstrateBy similarity
    Binding sitei248 – 2481SubstrateBy similarity
    Binding sitei250 – 2501Substrate; via carbonyl oxygenBy similarity
    Binding sitei259 – 2591SubstrateBy similarity
    Metal bindingi284 – 2841PotassiumBy similarity
    Metal bindingi292 – 2921MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi132 – 1376ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. methionine adenosyltransferase activity Source: MGI

    GO - Biological processi

    1. one-carbon metabolic process Source: UniProtKB-KW
    2. S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Enzyme and pathway databases

    ReactomeiREACT_202492. Sulfur amino acid metabolism.
    UniPathwayiUPA00315; UER00080.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-adenosylmethionine synthase isoform type-1 (EC:2.5.1.6)
    Short name:
    AdoMet synthase 1
    Alternative name(s):
    Methionine adenosyltransferase 1
    Short name:
    MAT 1
    Gene namesi
    Name:Mat1a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:88017. Mat1a.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396S-adenosylmethionine synthase isoform type-1PRO_0000174433Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 61By similarity
    Modified residuei121 – 1211S-nitrosocysteineBy similarity

    Post-translational modificationi

    S-nitrosylation of Cys-121 inactivates the enzyme.By similarity

    Keywords - PTMi

    Disulfide bond, S-nitrosylation

    Proteomic databases

    MaxQBiQ91X83.
    PaxDbiQ91X83.
    PRIDEiQ91X83.

    2D gel databases

    REPRODUCTION-2DPAGEQ91X83.

    PTM databases

    PhosphoSiteiQ91X83.

    Expressioni

    Gene expression databases

    BgeeiQ91X83.
    CleanExiMM_MAT1A.
    GenevestigatoriQ91X83.

    Interactioni

    Subunit structurei

    Homotetramer or homodimer.By similarity

    Protein-protein interaction databases

    DIPiDIP-45874N.
    IntActiQ91X83. 3 interactions.
    MINTiMINT-1855350.
    STRINGi10090.ENSMUSP00000044288.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91X83.
    SMRiQ91X83. Positions 17-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AdoMet synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0192.
    GeneTreeiENSGT00500000044811.
    HOGENOMiHOG000245710.
    HOVERGENiHBG001562.
    InParanoidiQ91X83.
    KOiK00789.
    OMAiITSHAIV.
    OrthoDBiEOG7TF79H.
    PhylomeDBiQ91X83.
    TreeFamiTF300511.

    Family and domain databases

    HAMAPiMF_00086. S_AdoMet_synth1.
    InterProiIPR022631. ADOMET_SYNTHASE_CS.
    IPR022630. S-AdoMet_synt_C.
    IPR022629. S-AdoMet_synt_central.
    IPR022628. S-AdoMet_synt_N.
    IPR002133. S-AdoMet_synthetase.
    IPR022636. S-AdoMet_synthetase_sfam.
    [Graphical view]
    PANTHERiPTHR11964. PTHR11964. 1 hit.
    PfamiPF02773. S-AdoMet_synt_C. 1 hit.
    PF02772. S-AdoMet_synt_M. 1 hit.
    PF00438. S-AdoMet_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000497. MAT. 1 hit.
    SUPFAMiSSF55973. SSF55973. 3 hits.
    TIGRFAMsiTIGR01034. metK. 1 hit.
    PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
    PS00377. ADOMET_SYNTHASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91X83-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD    50
    PNAKVACETV CKTGMVLLCG EITSVAMVDY QRVVRDTIKH IGYDDSAKGF 100
    DFKTCNVLVA LEQQSPDIAQ CVHLDRNEED VGAGDQGLMF GYATDETEEC 150
    MPLTIVLAHK LNTRIADLRR SGVLPWLRPD SKTQVTVQYM QDNGAVIPVR 200
    IHTIVISVQH NEDITLEAMQ EALKEQVIKA VVPAKYLDED TVYHLQPSGR 250
    FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR 300
    WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSN KTERELLEVV 350
    NKNFDLRPGV IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF 396
    Length:396
    Mass (Da):43,509
    Last modified:December 1, 2001 - v1
    Checksum:iAAA593A669551EB8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK149542 mRNA. Translation: BAE28947.1.
    AK153832 mRNA. Translation: BAE32202.1.
    BC011211 mRNA. Translation: AAH11211.1.
    CCDSiCCDS26959.1.
    PIRiA47151.
    RefSeqiNP_598414.1. NM_133653.3.
    XP_006518513.1. XM_006518450.1.
    UniGeneiMm.14064.
    Mm.486779.

    Genome annotation databases

    EnsembliENSMUST00000047286; ENSMUSP00000044288; ENSMUSG00000037798.
    GeneIDi11720.
    KEGGimmu:11720.
    UCSCiuc007tcm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK149542 mRNA. Translation: BAE28947.1 .
    AK153832 mRNA. Translation: BAE32202.1 .
    BC011211 mRNA. Translation: AAH11211.1 .
    CCDSi CCDS26959.1.
    PIRi A47151.
    RefSeqi NP_598414.1. NM_133653.3.
    XP_006518513.1. XM_006518450.1.
    UniGenei Mm.14064.
    Mm.486779.

    3D structure databases

    ProteinModelPortali Q91X83.
    SMRi Q91X83. Positions 17-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-45874N.
    IntActi Q91X83. 3 interactions.
    MINTi MINT-1855350.
    STRINGi 10090.ENSMUSP00000044288.

    PTM databases

    PhosphoSitei Q91X83.

    2D gel databases

    REPRODUCTION-2DPAGE Q91X83.

    Proteomic databases

    MaxQBi Q91X83.
    PaxDbi Q91X83.
    PRIDEi Q91X83.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047286 ; ENSMUSP00000044288 ; ENSMUSG00000037798 .
    GeneIDi 11720.
    KEGGi mmu:11720.
    UCSCi uc007tcm.1. mouse.

    Organism-specific databases

    CTDi 4143.
    MGIi MGI:88017. Mat1a.

    Phylogenomic databases

    eggNOGi COG0192.
    GeneTreei ENSGT00500000044811.
    HOGENOMi HOG000245710.
    HOVERGENi HBG001562.
    InParanoidi Q91X83.
    KOi K00789.
    OMAi ITSHAIV.
    OrthoDBi EOG7TF79H.
    PhylomeDBi Q91X83.
    TreeFami TF300511.

    Enzyme and pathway databases

    UniPathwayi UPA00315 ; UER00080 .
    Reactomei REACT_202492. Sulfur amino acid metabolism.

    Miscellaneous databases

    NextBioi 279397.
    PROi Q91X83.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91X83.
    CleanExi MM_MAT1A.
    Genevestigatori Q91X83.

    Family and domain databases

    HAMAPi MF_00086. S_AdoMet_synth1.
    InterProi IPR022631. ADOMET_SYNTHASE_CS.
    IPR022630. S-AdoMet_synt_C.
    IPR022629. S-AdoMet_synt_central.
    IPR022628. S-AdoMet_synt_N.
    IPR002133. S-AdoMet_synthetase.
    IPR022636. S-AdoMet_synthetase_sfam.
    [Graphical view ]
    PANTHERi PTHR11964. PTHR11964. 1 hit.
    Pfami PF02773. S-AdoMet_synt_C. 1 hit.
    PF02772. S-AdoMet_synt_M. 1 hit.
    PF00438. S-AdoMet_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000497. MAT. 1 hit.
    SUPFAMi SSF55973. SSF55973. 3 hits.
    TIGRFAMsi TIGR01034. metK. 1 hit.
    PROSITEi PS00376. ADOMET_SYNTHASE_1. 1 hit.
    PS00377. ADOMET_SYNTHASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of murine S-adenosylmethionine synthetase."
      Sakata S.F., Shelly L.L., Ruppert S., Schutz G., Chou J.Y.
      J. Biol. Chem. 268:13978-13986(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiMETK1_MOUSE
    AccessioniPrimary (citable) accession number: Q91X83
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3