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Reviewed, UniProtKB/Swiss-Prot Q91X83 (METK1_MOUSE)

Last modified February 9, 2010. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    S-adenosylmethionine synthetase isoform type-1
      Short name=AdoMet synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
      Short name=MAT 1
Gene names
Name: Mat1a
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.

Catalytic activity

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.

Cofactor

Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.

Binds 1 potassium ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

Subunit structure

Homotetramer or homodimer By similarity.

Post-translational modification

S-nitrosylation of Cys-121 inactivates the enzyme By similarity.

Sequence similarities

Belongs to the AdoMet synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396S-adenosylmethionine synthetase isoform type-1
PRO_0000174433

Regions

Nucleotide binding132 – 1376ATP Potential

Sites

Metal binding321Magnesium By similarity
Metal binding581Potassium By similarity
Metal binding2841Potassium By similarity
Metal binding2921Magnesium By similarity
Binding site301Substrate By similarity
Binding site1601ATP Potential
Binding site1801Substrate By similarity
Binding site2481Substrate By similarity
Binding site2501Substrate; via carbonyl oxygen By similarity
Binding site2591Substrate By similarity

Amino acid modifications

Modified residue1211S-nitrosocysteine By similarity
Disulfide bond35 ↔ 61 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q91X83-1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: AAA593A669551EB8

FASTA39643,509
        10         20         30         40         50         60 
MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD PNAKVACETV 

        70         80         90        100        110        120 
CKTGMVLLCG EITSVAMVDY QRVVRDTIKH IGYDDSAKGF DFKTCNVLVA LEQQSPDIAQ 

       130        140        150        160        170        180 
CVHLDRNEED VGAGDQGLMF GYATDETEEC MPLTIVLAHK LNTRIADLRR SGVLPWLRPD 

       190        200        210        220        230        240 
SKTQVTVQYM QDNGAVIPVR IHTIVISVQH NEDITLEAMQ EALKEQVIKA VVPAKYLDED 

       250        260        270        280        290        300 
TVYHLQPSGR FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR 

       310        320        330        340        350        360 
WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSN KTERELLEVV NKNFDLRPGV 

       370        380        390 
IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of murine S-adenosylmethionine synthetase."
Sakata S.F., Shelly L.L., Ruppert S., Schutz G., Chou J.Y.
J. Biol. Chem. 268:13978-13986(1993) [PubMed: 8314764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Liver and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK149542 mRNA. Translation: BAE28947.1.
AK153832 mRNA. Translation: BAE32202.1.
BC011211 mRNA. Translation: AAH11211.1.
IPIIPI00128518.
PIRA47151.
RefSeqNP_598414.1.
UniGeneMm.14064

3D structure databases

SMRQ91X83. Positions 17-396.
ModBaseSearch...

Protein-protein interaction databases

IntActQ91X83. 1 interaction.
STRINGQ91X83.

PTM databases

PhosphoSiteQ91X83.

2-D gel databases

REPRODUCTION-2DPAGEQ91X83.

Proteomic databases

PRIDEQ91X83.

Genome annotation databases

EnsemblENSMUST00000047286; ENSMUSP00000044288; ENSMUSG00000037798; Mus musculus. [Genome view]
GeneID11720.
KEGGmmu:11720.
NMPDRfig|10090.3.peg.28872.
UCSCuc007tcm.1. mouse.

Organism-specific databases

CTD11720.
MGIMGI:88017. Mat1a.

Phylogenomic databases

eggNOGroNOG08147.
HOGENOMHBG443662.
HOVERGENQ91X83.
InParanoidQ91X83.
OMADKDTIYH.
OrthoDBEOG95F07R.
PhylomeDBQ91X83.

Enzyme and pathway databases

BRENDA2.5.1.6. 244.

Gene expression databases

ArrayExpressQ91X83.
BgeeQ91X83.
CleanExMM_MAT1A.
GenevestigatorQ91X83.
GermOnlineENSMUSG00000037798. Mus musculus.

Family and domain databases

InterProIPR002133. S-AdoMet_synthetase.
[Graphical view]
PANTHERPTHR11964. S-AdoMet_synt. 1 hit.
PfamPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000497. MAT. 1 hit.
TIGRFAMsTIGR01034. metK. 1 hit.
PROSITEPS00376. ADOMET_SYNTHETASE_1. 1 hit.
PS00377. ADOMET_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio279397.
SOURCESearch...

Entry information

Entry nameMETK1_MOUSE
AccessionPrimary (citable) accession number: Q91X83
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: December 1, 2001
Last modified: February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents