Q91X83 (METK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase isoform type-1
Short name=AdoMet synthase 1
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 1
Short name=MAT 1

Gene names

Name:

Mat1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer or homodimer By similarity.
Post-translational modification	S-nitrosylation of Cys-121 inactivates the enzyme By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
PTM	Disulfide bond S-nitrosylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Traceable author statement Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthase isoform type-1		PRO_0000174433
Regions
	Nucleotide binding	132 – 137	6	ATP Potential
Sites
	Metal binding	32	1	Magnesium By similarity
	Metal binding	58	1	Potassium By similarity
	Metal binding	284	1	Potassium By similarity
	Metal binding	292	1	Magnesium By similarity
	Binding site	30	1	Substrate By similarity
	Binding site	160	1	ATP Potential
	Binding site	180	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	250	1	Substrate; via carbonyl oxygen By similarity
	Binding site	259	1	Substrate By similarity
Amino acid modifications
	Modified residue	121	1	S-nitrosocysteine By similarity
	Disulfide bond	35 ↔ 61		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91X83 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AAA593A669551EB8	FASTA	396	43,509
	10 20 30 40 50 60 MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD PNAKVACETV 70 80 90 100 110 120 CKTGMVLLCG EITSVAMVDY QRVVRDTIKH IGYDDSAKGF DFKTCNVLVA LEQQSPDIAQ 130 140 150 160 170 180 CVHLDRNEED VGAGDQGLMF GYATDETEEC MPLTIVLAHK LNTRIADLRR SGVLPWLRPD 190 200 210 220 230 240 SKTQVTVQYM QDNGAVIPVR IHTIVISVQH NEDITLEAMQ EALKEQVIKA VVPAKYLDED 250 260 270 280 290 300 TVYHLQPSGR FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR 310 320 330 340 350 360 WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSN KTERELLEVV NKNFDLRPGV 370 380 390 IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of murine S-adenosylmethionine synthetase." Sakata S.F., Shelly L.L., Ruppert S., Schutz G., Chou J.Y. J. Biol. Chem. 268:13978-13986(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK149542 mRNA. Translation: BAE28947.1. AK153832 mRNA. Translation: BAE32202.1. BC011211 mRNA. Translation: AAH11211.1.
IPI	IPI00128518.
PIR	A47151.
RefSeq	NP_598414.1. NM_133653.3.
UniGene	Mm.14064.
3D structure databases
ProteinModelPortal	Q91X83.
SMR	Q91X83. Positions 17-396.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-45874N.
IntAct	Q91X83. 1 interaction.
STRING	10090.ENSMUSP00000044288.
PTM databases
PhosphoSite	Q91X83.
2D gel databases
REPRODUCTION-2DPAGE	Q91X83.
Proteomic databases
PaxDb	Q91X83.
PRIDE	Q91X83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047286; ENSMUSP00000044288; ENSMUSG00000037798.
GeneID	11720.
KEGG	mmu:11720.
UCSC	uc007tcm.1. mouse.
Organism-specific databases
CTD	4143.
MGI	MGI:88017. Mat1a.
Phylogenomic databases
eggNOG	COG0192.
GeneTree	ENSGT00500000044811.
HOGENOM	HOG000245710.
HOVERGEN	HBG001562.
InParanoid	Q91X83.
KO	K00789.
OMA	IYYAHRL.
OrthoDB	EOG4QVCC3.
Enzyme and pathway databases
UniPathway	UPA00315; UER00080.
Gene expression databases
Bgee	Q91X83.
CleanEx	MM_MAT1A.
Genevestigator	Q91X83.
GermOnline	ENSMUSG00000037798. Mus musculus.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. PTHR11964. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279397.
SOURCE	Search...

Entry information

Entry name

METK1_MOUSE

Accession

Primary (citable) accession number: Q91X83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program