Reviewed, UniProtKB/Swiss-Prot Q91X83 (METK1_MOUSE)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase isoform type-1
      Short name=AdoMet synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
      Short name=MAT 1

Gene names

Name:

Mat1a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer or homodimer By similarity.
Post-translational modification	S-nitrosylation of Cys-121 inactivates the enzyme By similarity.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
PTM	Disulfide bond S-nitrosylation
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Ref.1 Traceable author statement. Source: MGI potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthetase isoform type-1		PRO_0000174433
Regions
	Nucleotide binding	132 – 137	6	ATP Potential
Sites
	Metal binding	32	1	Magnesium By similarity
	Metal binding	58	1	Potassium By similarity
	Metal binding	284	1	Potassium By similarity
	Metal binding	292	1	Magnesium By similarity
	Binding site	30	1	Substrate By similarity
	Binding site	160	1	ATP Potential
	Binding site	180	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	250	1	Substrate; via carbonyl oxygen By similarity
	Binding site	259	1	Substrate By similarity
Amino acid modifications
	Modified residue	121	1	S-nitrosocysteine By similarity
	Disulfide bond	35 ↔ 61		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91X83-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AAA593A669551EB8	FASTA	396	43,509
	10 20 30 40 50 60 MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD PNAKVACETV 70 80 90 100 110 120 CKTGMVLLCG EITSVAMVDY QRVVRDTIKH IGYDDSAKGF DFKTCNVLVA LEQQSPDIAQ 130 140 150 160 170 180 CVHLDRNEED VGAGDQGLMF GYATDETEEC MPLTIVLAHK LNTRIADLRR SGVLPWLRPD 190 200 210 220 230 240 SKTQVTVQYM QDNGAVIPVR IHTIVISVQH NEDITLEAMQ EALKEQVIKA VVPAKYLDED 250 260 270 280 290 300 TVYHLQPSGR FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR 310 320 330 340 350 360 WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSN KTERELLEVV NKNFDLRPGV 370 380 390 IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of murine S-adenosylmethionine synthetase." Sakata S.F., Shelly L.L., Ruppert S., Schutz G., Chou J.Y. J. Biol. Chem. 268:13978-13986(1993) [PubMed: 8314764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK149542 mRNA. Translation: BAE28947.1. AK153832 mRNA. Translation: BAE32202.1. BC011211 mRNA. Translation: AAH11211.1.
IPI	IPI00128518.
PIR	A47151.
RefSeq	NP_598414.1.
UniGene	Mm.14064
3D structure databases
SMR	Q91X83. Positions 17-396.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q91X83. 1 interaction.
STRING	Q91X83.
PTM databases
PhosphoSite	Q91X83.
2-D gel databases
REPRODUCTION-2DPAGE	Q91X83.
Proteomic databases
PRIDE	Q91X83.
Genome annotation databases
Ensembl	ENSMUST00000047286; ENSMUSP00000044288; ENSMUSG00000037798; Mus musculus. [Genome view]
GeneID	11720.
KEGG	mmu:11720.
NMPDR	fig|10090.3.peg.28872.
UCSC	uc007tcm.1. mouse.
Organism-specific databases
CTD	11720.
MGI	MGI:88017. Mat1a.
Phylogenomic databases
eggNOG	roNOG08147.
HOGENOM	HBG443662.
HOVERGEN	Q91X83.
InParanoid	Q91X83.
OMA	DKDTIYH.
OrthoDB	EOG95F07R.
PhylomeDB	Q91X83.
Enzyme and pathway databases
BRENDA	2.5.1.6. 244.
Gene expression databases
ArrayExpress	Q91X83.
Bgee	Q91X83.
CleanEx	MM_MAT1A.
Genevestigator	Q91X83.
GermOnline	ENSMUSG00000037798. Mus musculus.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279397.
SOURCE	Search...

Entry information

Entry name

METK1_MOUSE

Accession

Primary (citable) accession number: Q91X83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)