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Q91X77

- CY250_MOUSE

UniProt

Q91X77 - CY250_MOUSE

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Protein

Cytochrome P450 2C50

Gene

Cyp2c50

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Metabolizes arachidonic acid to several midchain and omega-terminal hydroxyeicosatetraenoic acids (HETE).1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi435 – 4351Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: MGI
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. linoleic acid epoxygenase activity Source: MGI

GO - Biological processi

  1. arachidonic acid metabolic process Source: MGI
  2. linoleic acid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_245291. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C50 (EC:1.14.14.1)
Alternative name(s):
CYPIIC50
Gene namesi
Name:Cyp2c50Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:2147497. Cyp2c50.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication. Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Cytochrome P450 2C50PRO_0000282957Add
BLAST

Proteomic databases

MaxQBiQ91X77.
PaxDbiQ91X77.
PRIDEiQ91X77.

PTM databases

PhosphoSiteiQ91X77.

Expressioni

Tissue specificityi

Expressed in heart and liver.1 Publication

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.Curated

Gene expression databases

BgeeiQ91X77.
GenevestigatoriQ91X77.

Structurei

3D structure databases

ProteinModelPortaliQ91X77.
SMRiQ91X77. Positions 30-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ91X77.
KOiK07413.
OMAiTWINTNT.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q91X77-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPILVLVFT LSCLFLLSLW RQSSERGKLP PGPTPLPIIG NILQINVKDI
60 70 80 90 100
CQSFTNLSKV YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GEEFAGRGRL
110 120 130 140 150
PVFDKATNGM GIIFSKGNVW KNTRRFSLTT LRNLGMGKRS IEDRVQEEAR
160 170 180 190 200
CLVEELRKTN GSPCDPTFIL GCAPCNVICS IIFQDRFDYK DRDFLNLMEK
210 220 230 240 250
LNEITKIMST PWLQVCNTFP VLLDYCPGSH NKVFKNYACI KNFLLEKIKE
260 270 280 290 300
HEESLDVTIP RDFIDYFLIN GGQENGNYPL KNRLEHLAIT VTDLFSAGTE
310 320 330 340 350
TTSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTDA
360 370 380 390 400
MIHEVQRFID LVPNSLPHEV TCDIKFRNYF IPKGTNVITS LSSVLRDSKE
410 420 430 440 450
FPNPEKFDPG HFLDENGKFK KSDYFMPFST GKRICAGEGL ARMELFLFLT
460 470 480 490
SILQNFNLKP LVHPKDIDVT PMLIGLASVP PAFQLCFIPS
Length:490
Mass (Da):55,765
Last modified:July 27, 2011 - v2
Checksum:i6C66FA7482AB3F78
GO
Isoform 21 Publication (identifier: Q91X77-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-273: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:431
Mass (Da):48,932
Checksum:i1BA9595194DEAC01
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711R → N in AAH51050. (PubMed:15489334)Curated
Sequence conflicti71 – 711R → N in AAH11222. (PubMed:15489334)Curated
Sequence conflicti275 – 2751N → D in AAH11222. (PubMed:15489334)Curated
Sequence conflicti336 – 3361R → S in AAH51050. (PubMed:15489334)Curated
Sequence conflicti336 – 3361R → S in AAH11222. (PubMed:15489334)Curated
Sequence conflicti344 – 3441H → R in AAH51050. (PubMed:15489334)Curated
Sequence conflicti344 – 3441H → R in AAH11222. (PubMed:15489334)Curated
Sequence conflicti458 – 4581L → Q in AAO52736. (PubMed:15102943)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei215 – 27359Missing in isoform 2. 1 PublicationVSP_052374Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206873 mRNA. Translation: AAO52736.1.
AC148014 Genomic DNA. No translation available.
BC011222 mRNA. Translation: AAH11222.1.
BC051050 mRNA. Translation: AAH51050.1.
CCDSiCCDS29801.1. [Q91X77-1]
CCDS50433.1. [Q91X77-2]
RefSeqiNP_001161347.1. NM_001167875.1. [Q91X77-2]
NP_598905.2. NM_134144.2. [Q91X77-1]
UniGeneiMm.474575.

Genome annotation databases

EnsembliENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827. [Q91X77-2]
ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827. [Q91X77-1]
GeneIDi107141.
KEGGimmu:107141.
UCSCiuc008hki.2. mouse. [Q91X77-1]
uc012blo.1. mouse. [Q91X77-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206873 mRNA. Translation: AAO52736.1 .
AC148014 Genomic DNA. No translation available.
BC011222 mRNA. Translation: AAH11222.1 .
BC051050 mRNA. Translation: AAH51050.1 .
CCDSi CCDS29801.1. [Q91X77-1 ]
CCDS50433.1. [Q91X77-2 ]
RefSeqi NP_001161347.1. NM_001167875.1. [Q91X77-2 ]
NP_598905.2. NM_134144.2. [Q91X77-1 ]
UniGenei Mm.474575.

3D structure databases

ProteinModelPortali Q91X77.
SMRi Q91X77. Positions 30-489.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q91X77.

Proteomic databases

MaxQBi Q91X77.
PaxDbi Q91X77.
PRIDEi Q91X77.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068094 ; ENSMUSP00000068039 ; ENSMUSG00000054827 . [Q91X77-2 ]
ENSMUST00000080171 ; ENSMUSP00000079065 ; ENSMUSG00000054827 . [Q91X77-1 ]
GeneIDi 107141.
KEGGi mmu:107141.
UCSCi uc008hki.2. mouse. [Q91X77-1 ]
uc012blo.1. mouse. [Q91X77-2 ]

Organism-specific databases

CTDi 107141.
MGIi MGI:2147497. Cyp2c50.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118775.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi Q91X77.
KOi K07413.
OMAi TWINTNT.
OrthoDBi EOG7RBZ85.
TreeFami TF352043.

Enzyme and pathway databases

Reactomei REACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_245291. Xenobiotics.

Miscellaneous databases

NextBioi 358582.
PROi Q91X77.
SOURCEi Search...

Gene expression databases

Bgeei Q91X77.
Genevestigatori Q91X77.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and characterization of three new mouse cytochrome p450 enzymes and partial characterization of their fatty acid oxidation activities."
    Wang H., Zhao Y., Bradbury J.A., Graves J.P., Foley J., Blaisdell J.A., Goldstein J.A., Zeldin D.C.
    Mol. Pharmacol. 65:1148-1158(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM 1).
    Strain: C57BL/6Imported.
    Tissue: Heart1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/NImported.
    Tissue: LiverImported.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 100-116, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiCY250_MOUSE
AccessioniPrimary (citable) accession number: Q91X77
Secondary accession number(s): E9QPJ0, Q6XVG3, Q80X43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3