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Protein

Cytochrome P450 2C50

Gene

Cyp2c50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metabolizes arachidonic acid to several midchain and omega-terminal hydroxyeicosatetraenoic acids (HETE).1 Publication

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi435Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • arachidonic acid epoxygenase activity Source: MGI
  • aromatase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • linoleic acid epoxygenase activity Source: MGI
  • steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  • arachidonic acid metabolic process Source: MGI
  • epoxygenase P450 pathway Source: GO_Central
  • linoleic acid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C50 (EC:1.14.14.11 Publication)
Alternative name(s):
CYPIIC50
Gene namesi
Name:Cyp2c50Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147497. Cyp2c50.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002829571 – 490Cytochrome P450 2C50Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei249N6-acetyllysineBy similarity1
Modified residuei375N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ91X77.
PeptideAtlasiQ91X77.
PRIDEiQ91X77.

PTM databases

iPTMnetiQ91X77.
PhosphoSitePlusiQ91X77.
SwissPalmiQ91X77.

Expressioni

Tissue specificityi

Expressed in heart and liver.1 Publication

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.Curated

Gene expression databases

BgeeiENSMUSG00000054827.
GenevisibleiQ91X77. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079065.

Structurei

3D structure databases

ProteinModelPortaliQ91X77.
SMRiQ91X77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ91X77.
KOiK07413.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q91X77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPILVLVFT LSCLFLLSLW RQSSERGKLP PGPTPLPIIG NILQINVKDI
60 70 80 90 100
CQSFTNLSKV YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GEEFAGRGRL
110 120 130 140 150
PVFDKATNGM GIIFSKGNVW KNTRRFSLTT LRNLGMGKRS IEDRVQEEAR
160 170 180 190 200
CLVEELRKTN GSPCDPTFIL GCAPCNVICS IIFQDRFDYK DRDFLNLMEK
210 220 230 240 250
LNEITKIMST PWLQVCNTFP VLLDYCPGSH NKVFKNYACI KNFLLEKIKE
260 270 280 290 300
HEESLDVTIP RDFIDYFLIN GGQENGNYPL KNRLEHLAIT VTDLFSAGTE
310 320 330 340 350
TTSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTDA
360 370 380 390 400
MIHEVQRFID LVPNSLPHEV TCDIKFRNYF IPKGTNVITS LSSVLRDSKE
410 420 430 440 450
FPNPEKFDPG HFLDENGKFK KSDYFMPFST GKRICAGEGL ARMELFLFLT
460 470 480 490
SILQNFNLKP LVHPKDIDVT PMLIGLASVP PAFQLCFIPS
Length:490
Mass (Da):55,765
Last modified:July 27, 2011 - v2
Checksum:i6C66FA7482AB3F78
GO
Isoform 21 Publication (identifier: Q91X77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-273: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:431
Mass (Da):48,932
Checksum:i1BA9595194DEAC01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71R → N in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti71R → N in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti275N → D in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti336R → S in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti336R → S in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti344H → R in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti344H → R in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti458L → Q in AAO52736 (PubMed:15102943).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052374215 – 273Missing in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206873 mRNA. Translation: AAO52736.1.
AC148014 Genomic DNA. No translation available.
BC011222 mRNA. Translation: AAH11222.1.
BC051050 mRNA. Translation: AAH51050.1.
CCDSiCCDS29801.1. [Q91X77-1]
CCDS50433.1. [Q91X77-2]
RefSeqiNP_001161347.1. NM_001167875.1. [Q91X77-2]
NP_598905.2. NM_134144.2. [Q91X77-1]
UniGeneiMm.474575.

Genome annotation databases

EnsembliENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827. [Q91X77-2]
ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827. [Q91X77-1]
GeneIDi107141.
KEGGimmu:107141.
UCSCiuc008hki.2. mouse. [Q91X77-1]
uc012blo.1. mouse. [Q91X77-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206873 mRNA. Translation: AAO52736.1.
AC148014 Genomic DNA. No translation available.
BC011222 mRNA. Translation: AAH11222.1.
BC051050 mRNA. Translation: AAH51050.1.
CCDSiCCDS29801.1. [Q91X77-1]
CCDS50433.1. [Q91X77-2]
RefSeqiNP_001161347.1. NM_001167875.1. [Q91X77-2]
NP_598905.2. NM_134144.2. [Q91X77-1]
UniGeneiMm.474575.

3D structure databases

ProteinModelPortaliQ91X77.
SMRiQ91X77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079065.

PTM databases

iPTMnetiQ91X77.
PhosphoSitePlusiQ91X77.
SwissPalmiQ91X77.

Proteomic databases

PaxDbiQ91X77.
PeptideAtlasiQ91X77.
PRIDEiQ91X77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827. [Q91X77-2]
ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827. [Q91X77-1]
GeneIDi107141.
KEGGimmu:107141.
UCSCiuc008hki.2. mouse. [Q91X77-1]
uc012blo.1. mouse. [Q91X77-2]

Organism-specific databases

CTDi107141.
MGIiMGI:2147497. Cyp2c50.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiQ91X77.
KOiK07413.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Miscellaneous databases

PROiQ91X77.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054827.
GenevisibleiQ91X77. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCY250_MOUSE
AccessioniPrimary (citable) accession number: Q91X77
Secondary accession number(s): E9QPJ0, Q6XVG3, Q80X43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.