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Q91X77 (CY250_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2C50
EC=1.14.14.1
Alternative name(s):
CYPIIC50
Gene names
Name:Cyp2c50
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Metabolizes arachidonic acid to several midchain and omega-terminal hydroxyeicosatetraenoic acids (HETE). Ref.1

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. Ref.1

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein Ref.1.

Tissue specificity

Expressed in heart and liver. Ref.1

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q91X77-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: Q91X77-2)

The sequence of this isoform differs from the canonical sequence as follows:
     215-273: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Cytochrome P450 2C50
PRO_0000282957

Sites

Metal binding4351Iron (heme axial ligand) By similarity UniProtKB P10632

Natural variations

Alternative sequence215 – 27359Missing in isoform 2. Ref.3
VSP_052374

Experimental info

Sequence conflict711R → N in AAH51050. Ref.3
Sequence conflict711R → N in AAH11222. Ref.3
Sequence conflict2751N → D in AAH11222. Ref.3
Sequence conflict3361R → S in AAH51050. Ref.3
Sequence conflict3361R → S in AAH11222. Ref.3
Sequence conflict3441H → R in AAH51050. Ref.3
Sequence conflict3441H → R in AAH11222. Ref.3
Sequence conflict4581L → Q in AAO52736. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 6C66FA7482AB3F78

FASTA49055,765
        10         20         30         40         50         60 
MDPILVLVFT LSCLFLLSLW RQSSERGKLP PGPTPLPIIG NILQINVKDI CQSFTNLSKV 

        70         80         90        100        110        120 
YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GEEFAGRGRL PVFDKATNGM GIIFSKGNVW 

       130        140        150        160        170        180 
KNTRRFSLTT LRNLGMGKRS IEDRVQEEAR CLVEELRKTN GSPCDPTFIL GCAPCNVICS 

       190        200        210        220        230        240 
IIFQDRFDYK DRDFLNLMEK LNEITKIMST PWLQVCNTFP VLLDYCPGSH NKVFKNYACI 

       250        260        270        280        290        300 
KNFLLEKIKE HEESLDVTIP RDFIDYFLIN GGQENGNYPL KNRLEHLAIT VTDLFSAGTE 

       310        320        330        340        350        360 
TTSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTDA MIHEVQRFID 

       370        380        390        400        410        420 
LVPNSLPHEV TCDIKFRNYF IPKGTNVITS LSSVLRDSKE FPNPEKFDPG HFLDENGKFK 

       430        440        450        460        470        480 
KSDYFMPFST GKRICAGEGL ARMELFLFLT SILQNFNLKP LVHPKDIDVT PMLIGLASVP 

       490 
PAFQLCFIPS

« Hide

Isoform 2 [UniParc].

Checksum: 1BA9595194DEAC01
Show »

FASTA43148,932

References

« Hide 'large scale' references
[1]"Cloning, expression, and characterization of three new mouse cytochrome p450 enzymes and partial characterization of their fatty acid oxidation activities."
Wang H., Zhao Y., Bradbury J.A., Graves J.P., Foley J., Blaisdell J.A., Goldstein J.A., Zeldin D.C.
Mol. Pharmacol. 65:1148-1158(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM 1).
Strain: C57BL/6.
Tissue: Heart.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Liver.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 100-116, MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY206873 mRNA. Translation: AAO52736.1.
AC148014 Genomic DNA. No translation available.
BC011222 mRNA. Translation: AAH11222.1.
BC051050 mRNA. Translation: AAH51050.1.
IPIIPI00128489.
IPI00403701.
RefSeqNP_001161347.1. NM_001167875.1.
NP_598905.2. NM_134144.2.
UniGeneMm.474575.

3D structure databases

HSSPHSSP built from PDB template 1R9O based on UniProtKB P11712.
ProteinModelPortalQ91X77.
SMRQ91X77. Positions 30-489.
ModBaseSearch...

PTM databases

PhosphoSiteQ91X77.

Proteomic databases

PaxDbQ91X77.
PRIDEQ91X77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827.
ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827.
GeneID107141.
KEGGmmu:107141.
UCSCuc008hkf.2. mouse.
uc012bln.1. mouse.

Organism-specific databases

CTD107141.
MGIMGI:2147497. Cyp2c50.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00680000099783.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidQ91X77.
KOK07413.
OrthoDBEOG48WC22.

Gene expression databases

BgeeQ91X77.
GenevestigatorQ91X77.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio358582.
SOURCESearch...

Entry information

Entry nameCY250_MOUSE
AccessionPrimary (citable) accession number: Q91X77
Secondary accession number(s): E9QPJ0, Q6XVG3, Q80X43
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents