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Q91X77

- CY250_MOUSE

UniProt

Q91X77 - CY250_MOUSE

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Protein
Cytochrome P450 2C50
Gene
Cyp2c50
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Metabolizes arachidonic acid to several midchain and omega-terminal hydroxyeicosatetraenoic acids (HETE).1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.1 Publication

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi435 – 4351Iron (heme axial ligand) By similarityBy similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2C50 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIC50
    Gene namesi
    Name:Cyp2c50
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:2147497. Cyp2c50.

    Subcellular locationi

    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Cytochrome P450 2C50
    PRO_0000282957Add
    BLAST

    Proteomic databases

    MaxQBiQ91X77.
    PaxDbiQ91X77.
    PRIDEiQ91X77.

    PTM databases

    PhosphoSiteiQ91X77.

    Expressioni

    Tissue specificityi

    Expressed in heart and liver.1 Publication

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    BgeeiQ91X77.
    GenevestigatoriQ91X77.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91X77.
    SMRiQ91X77. Positions 30-489.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00680000099783.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiQ91X77.
    KOiK07413.
    OMAiTWINTNT.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q91X77-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPILVLVFT LSCLFLLSLW RQSSERGKLP PGPTPLPIIG NILQINVKDI    50
    CQSFTNLSKV YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GEEFAGRGRL 100
    PVFDKATNGM GIIFSKGNVW KNTRRFSLTT LRNLGMGKRS IEDRVQEEAR 150
    CLVEELRKTN GSPCDPTFIL GCAPCNVICS IIFQDRFDYK DRDFLNLMEK 200
    LNEITKIMST PWLQVCNTFP VLLDYCPGSH NKVFKNYACI KNFLLEKIKE 250
    HEESLDVTIP RDFIDYFLIN GGQENGNYPL KNRLEHLAIT VTDLFSAGTE 300
    TTSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTDA 350
    MIHEVQRFID LVPNSLPHEV TCDIKFRNYF IPKGTNVITS LSSVLRDSKE 400
    FPNPEKFDPG HFLDENGKFK KSDYFMPFST GKRICAGEGL ARMELFLFLT 450
    SILQNFNLKP LVHPKDIDVT PMLIGLASVP PAFQLCFIPS 490
    Length:490
    Mass (Da):55,765
    Last modified:July 27, 2011 - v2
    Checksum:i6C66FA7482AB3F78
    GO
    Isoform 21 Publication (identifier: Q91X77-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         215-273: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:431
    Mass (Da):48,932
    Checksum:i1BA9595194DEAC01
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei215 – 27359Missing in isoform 2. 1 Publication
    VSP_052374Add
    BLAST

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711R → N in AAH51050. 1 Publication
    Sequence conflicti71 – 711R → N in AAH11222. 1 Publication
    Sequence conflicti275 – 2751N → D in AAH11222. 1 Publication
    Sequence conflicti336 – 3361R → S in AAH51050. 1 Publication
    Sequence conflicti336 – 3361R → S in AAH11222. 1 Publication
    Sequence conflicti344 – 3441H → R in AAH51050. 1 Publication
    Sequence conflicti344 – 3441H → R in AAH11222. 1 Publication
    Sequence conflicti458 – 4581L → Q in AAO52736. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY206873 mRNA. Translation: AAO52736.1.
    AC148014 Genomic DNA. No translation available.
    BC011222 mRNA. Translation: AAH11222.1.
    BC051050 mRNA. Translation: AAH51050.1.
    CCDSiCCDS29801.1. [Q91X77-1]
    CCDS50433.1. [Q91X77-2]
    RefSeqiNP_001161347.1. NM_001167875.1. [Q91X77-2]
    NP_598905.2. NM_134144.2. [Q91X77-1]
    UniGeneiMm.474575.

    Genome annotation databases

    EnsembliENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827. [Q91X77-2]
    ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827. [Q91X77-1]
    GeneIDi107141.
    KEGGimmu:107141.
    UCSCiuc008hki.2. mouse. [Q91X77-1]
    uc012blo.1. mouse. [Q91X77-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY206873 mRNA. Translation: AAO52736.1 .
    AC148014 Genomic DNA. No translation available.
    BC011222 mRNA. Translation: AAH11222.1 .
    BC051050 mRNA. Translation: AAH51050.1 .
    CCDSi CCDS29801.1. [Q91X77-1 ]
    CCDS50433.1. [Q91X77-2 ]
    RefSeqi NP_001161347.1. NM_001167875.1. [Q91X77-2 ]
    NP_598905.2. NM_134144.2. [Q91X77-1 ]
    UniGenei Mm.474575.

    3D structure databases

    ProteinModelPortali Q91X77.
    SMRi Q91X77. Positions 30-489.
    ModBasei Search...

    PTM databases

    PhosphoSitei Q91X77.

    Proteomic databases

    MaxQBi Q91X77.
    PaxDbi Q91X77.
    PRIDEi Q91X77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068094 ; ENSMUSP00000068039 ; ENSMUSG00000054827 . [Q91X77-2 ]
    ENSMUST00000080171 ; ENSMUSP00000079065 ; ENSMUSG00000054827 . [Q91X77-1 ]
    GeneIDi 107141.
    KEGGi mmu:107141.
    UCSCi uc008hki.2. mouse. [Q91X77-1 ]
    uc012blo.1. mouse. [Q91X77-2 ]

    Organism-specific databases

    CTDi 107141.
    MGIi MGI:2147497. Cyp2c50.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00680000099783.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi Q91X77.
    KOi K07413.
    OMAi TWINTNT.
    OrthoDBi EOG7RBZ85.
    TreeFami TF352043.

    Enzyme and pathway databases

    Reactomei REACT_196570. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

    Miscellaneous databases

    NextBioi 358582.
    PROi Q91X77.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91X77.
    Genevestigatori Q91X77.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning, expression, and characterization of three new mouse cytochrome p450 enzymes and partial characterization of their fatty acid oxidation activities."
      Wang H., Zhao Y., Bradbury J.A., Graves J.P., Foley J., Blaisdell J.A., Goldstein J.A., Zeldin D.C.
      Mol. Pharmacol. 65:1148-1158(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Heart.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Liver.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 100-116, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiCY250_MOUSE
    AccessioniPrimary (citable) accession number: Q91X77
    Secondary accession number(s): E9QPJ0, Q6XVG3, Q80X43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: July 27, 2011
    Last modified: September 3, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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