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Protein

Cytochrome P450 2C50

Gene

Cyp2c50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metabolizes arachidonic acid to several midchain and omega-terminal hydroxyeicosatetraenoic acids (HETE).1 Publication

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi435Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • arachidonic acid epoxygenase activity Source: MGI
  • aromatase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • linoleic acid epoxygenase activity Source: MGI
  • steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  • arachidonic acid metabolic process Source: MGI
  • epoxygenase P450 pathway Source: GO_Central
  • linoleic acid metabolic process Source: MGI

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-211981 Xenobiotics
R-MMU-211999 CYP2E1 reactions
R-MMU-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
R-MMU-2142816 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
R-MMU-9027307 Biosynthesis of maresin-like SPMs

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C50 (EC:1.14.14.11 Publication)
Alternative name(s):
CYPIIC50
Gene namesi
Name:Cyp2c50Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2147497 Cyp2c50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002829571 – 490Cytochrome P450 2C50Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei127PhosphoserineBy similarity1
Modified residuei249N6-acetyllysineBy similarity1
Modified residuei375N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ91X77
PeptideAtlasiQ91X77
PRIDEiQ91X77

PTM databases

iPTMnetiQ91X77
PhosphoSitePlusiQ91X77
SwissPalmiQ91X77

Expressioni

Tissue specificityi

Expressed in heart and liver.1 Publication

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.Curated

Gene expression databases

BgeeiENSMUSG00000054827
GenevisibleiQ91X77 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079065

Structurei

3D structure databases

ProteinModelPortaliQ91X77
SMRiQ91X77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00880000137861
HOGENOMiHOG000036992
HOVERGENiHBG015789
InParanoidiQ91X77
KOiK07413
OMAiELINHPP
OrthoDBiEOG091G0BT8
TreeFamiTF352043

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q91X77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPILVLVFT LSCLFLLSLW RQSSERGKLP PGPTPLPIIG NILQINVKDI
60 70 80 90 100
CQSFTNLSKV YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GEEFAGRGRL
110 120 130 140 150
PVFDKATNGM GIIFSKGNVW KNTRRFSLTT LRNLGMGKRS IEDRVQEEAR
160 170 180 190 200
CLVEELRKTN GSPCDPTFIL GCAPCNVICS IIFQDRFDYK DRDFLNLMEK
210 220 230 240 250
LNEITKIMST PWLQVCNTFP VLLDYCPGSH NKVFKNYACI KNFLLEKIKE
260 270 280 290 300
HEESLDVTIP RDFIDYFLIN GGQENGNYPL KNRLEHLAIT VTDLFSAGTE
310 320 330 340 350
TTSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTDA
360 370 380 390 400
MIHEVQRFID LVPNSLPHEV TCDIKFRNYF IPKGTNVITS LSSVLRDSKE
410 420 430 440 450
FPNPEKFDPG HFLDENGKFK KSDYFMPFST GKRICAGEGL ARMELFLFLT
460 470 480 490
SILQNFNLKP LVHPKDIDVT PMLIGLASVP PAFQLCFIPS
Length:490
Mass (Da):55,765
Last modified:July 27, 2011 - v2
Checksum:i6C66FA7482AB3F78
GO
Isoform 21 Publication (identifier: Q91X77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-273: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:431
Mass (Da):48,932
Checksum:i1BA9595194DEAC01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71R → N in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti71R → N in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti275N → D in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti336R → S in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti336R → S in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti344H → R in AAH51050 (PubMed:15489334).Curated1
Sequence conflicti344H → R in AAH11222 (PubMed:15489334).Curated1
Sequence conflicti458L → Q in AAO52736 (PubMed:15102943).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052374215 – 273Missing in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206873 mRNA Translation: AAO52736.1
AC148014 Genomic DNA No translation available.
BC011222 mRNA Translation: AAH11222.1
BC051050 mRNA Translation: AAH51050.1
CCDSiCCDS29801.1 [Q91X77-1]
CCDS50433.1 [Q91X77-2]
RefSeqiNP_001161347.1, NM_001167875.1 [Q91X77-2]
NP_598905.2, NM_134144.2 [Q91X77-1]
UniGeneiMm.474575

Genome annotation databases

EnsembliENSMUST00000068094; ENSMUSP00000068039; ENSMUSG00000054827 [Q91X77-2]
ENSMUST00000080171; ENSMUSP00000079065; ENSMUSG00000054827 [Q91X77-1]
GeneIDi107141
KEGGimmu:107141
UCSCiuc008hki.2 mouse [Q91X77-1]
uc012blo.1 mouse [Q91X77-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCY250_MOUSE
AccessioniPrimary (citable) accession number: Q91X77
Secondary accession number(s): E9QPJ0, Q6XVG3, Q80X43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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