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Protein

Hemopexin

Gene

Hpx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Iron (heme 1 axial ligand)By similarity
Metal bindingi149 – 1491Iron (heme 1 axial ligand)By similarity
Metal bindingi235 – 2351Iron (heme 2 axial ligand)By similarity
Metal bindingi291 – 2911Iron (heme 2 axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. heme metabolic process Source: MGI
  2. hemoglobin metabolic process Source: MGI
  3. positive regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  4. positive regulation of immunoglobulin production Source: MGI
  5. positive regulation of interferon-gamma-mediated signaling pathway Source: MGI
  6. positive regulation of response to interferon-gamma Source: MGI
  7. positive regulation of tyrosine phosphorylation of Stat1 protein Source: MGI
  8. regulation of protein metabolic process Source: MGI
  9. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196575. Scavenging of heme from plasma.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemopexin
Gene namesi
Name:Hpx
Synonyms:Hpxn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:105112. Hpx.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: MGI
  2. extracellular space Source: MGI
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 460437HemopexinPRO_0000021407Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)1 Publication
Disulfide bondi50 ↔ 230By similarity
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi148 ↔ 153By similarity
Glycosylationi186 – 1861N-linked (GlcNAc...)1 Publication
Disulfide bondi187 ↔ 199By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi255 ↔ 458By similarity
Disulfide bondi364 ↔ 406By similarity
Disulfide bondi416 ↔ 433By similarity
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ91X72.
PaxDbiQ91X72.
PRIDEiQ91X72.

PTM databases

PhosphoSiteiQ91X72.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiQ91X72.
CleanExiMM_HPX.
GenevestigatoriQ91X72.

Interactioni

Protein-protein interaction databases

BioGridi200414. 1 interaction.
IntActiQ91X72. 4 interactions.
MINTiMINT-1862286.

Structurei

3D structure databases

ProteinModelPortaliQ91X72.
SMRiQ91X72. Positions 48-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 9341Hemopexin 1Add
BLAST
Repeati94 – 13845Hemopexin 2Add
BLAST
Repeati139 – 18345Hemopexin 3Add
BLAST
Repeati184 – 23047Hemopexin 4Add
BLAST
Repeati257 – 30246Hemopexin 5Add
BLAST
Repeati303 – 35048Hemopexin 6Add
BLAST
Repeati355 – 39440Hemopexin 7Add
BLAST
Repeati398 – 44851Hemopexin 8Add
BLAST

Sequence similaritiesi

Belongs to the hemopexin family.Curated
Contains 8 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG251805.
GeneTreeiENSGT00390000009178.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiQ91X72.
OMAiAVECHRG.
OrthoDBiEOG7Q8CN5.
TreeFamiTF331201.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR016358. Hemopexin_chordata.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 5 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 6 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91X72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTAVALNI LVLLGLCWSL AVASPLPTAN GRVAEVENGT KPDSDVPEHC
60 70 80 90 100
LDTWSFDAAT MDHNGTMLFF KGEFVWRGHS GTRELISARW KNPITSVDAA
110 120 130 140 150
FRGPDSVFLI KEDKVWVYPP EKKENGYPKL FQEEFPGIPY PPDAAVECHR
160 170 180 190 200
GECQSEGVLF FQGNRKWFWD FATRTQKERS WSTVGNCTAA LRWLERYYCF
210 220 230 240 250
QGNKFLRFNP VTGEVPPRYP LDARDYFVSC PGRGHGRPRN GTAHGNSTHP
260 270 280 290 300
MHSRCSPDPG LTALLSDHRG ATYAFTGSHY WRLDSSRDGW HSWPIAHHWP
310 320 330 340 350
QGPSTVDAAF SWDDKVYLIQ GTQVYVFLTK GGNNLVSGYP KRLEKELGSP
360 370 380 390 400
PGISLETIDA AFSCPGSSRL YVSSGRRLWW LDLKSGAQAT WTEVSWPHEK
410 420 430 440 450
VDGALCLDKS LGPNTCSSNG SSLYFIHGPN LYCYSSIDKL NAAKSLPQPQ
460
KVNSILGCSQ
Length:460
Mass (Da):51,318
Last modified:July 27, 2011 - v2
Checksum:i5DF86BA50CC23B48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301N → H in AAH11246 (PubMed:15489334).Curated
Sequence conflicti30 – 301N → H in AAH19901 (PubMed:15489334).Curated
Sequence conflicti233 – 2331R → Q in AAB49490 (Ref. 4) Curated
Sequence conflicti453 – 4531Missing in AAB49490 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145928 mRNA. Translation: BAE26759.1.
CH466531 Genomic DNA. Translation: EDL16785.1.
BC011246 mRNA. Translation: AAH11246.1.
BC019901 mRNA. Translation: AAH19901.1.
U89889 mRNA. Translation: AAB49490.1.
CCDSiCCDS21654.1.
RefSeqiNP_059067.2. NM_017371.2.
UniGeneiMm.3485.

Genome annotation databases

EnsembliENSMUST00000033185; ENSMUSP00000033185; ENSMUSG00000030895.
GeneIDi15458.
KEGGimmu:15458.
UCSCiuc009iyl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145928 mRNA. Translation: BAE26759.1.
CH466531 Genomic DNA. Translation: EDL16785.1.
BC011246 mRNA. Translation: AAH11246.1.
BC019901 mRNA. Translation: AAH19901.1.
U89889 mRNA. Translation: AAB49490.1.
CCDSiCCDS21654.1.
RefSeqiNP_059067.2. NM_017371.2.
UniGeneiMm.3485.

3D structure databases

ProteinModelPortaliQ91X72.
SMRiQ91X72. Positions 48-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200414. 1 interaction.
IntActiQ91X72. 4 interactions.
MINTiMINT-1862286.

PTM databases

PhosphoSiteiQ91X72.

Proteomic databases

MaxQBiQ91X72.
PaxDbiQ91X72.
PRIDEiQ91X72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033185; ENSMUSP00000033185; ENSMUSG00000030895.
GeneIDi15458.
KEGGimmu:15458.
UCSCiuc009iyl.2. mouse.

Organism-specific databases

CTDi3263.
MGIiMGI:105112. Hpx.

Phylogenomic databases

eggNOGiNOG251805.
GeneTreeiENSGT00390000009178.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiQ91X72.
OMAiAVECHRG.
OrthoDBiEOG7Q8CN5.
TreeFamiTF331201.

Enzyme and pathway databases

ReactomeiREACT_196575. Scavenging of heme from plasma.

Miscellaneous databases

ChiTaRSiHpx. mouse.
NextBioi288270.
PROiQ91X72.
SOURCEiSearch...

Gene expression databases

BgeeiQ91X72.
CleanExiMM_HPX.
GenevestigatoriQ91X72.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR016358. Hemopexin_chordata.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 5 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 6 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. Koepsel R.R., Rohrbach D.H., Brekheiser B.B.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-460.
    Tissue: Liver.
  5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-38.
    Strain: C57BL/6.
    Tissue: Plasma.
  6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-186.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiHEMO_MOUSE
AccessioniPrimary (citable) accession number: Q91X72
Secondary accession number(s): P97824, Q3UKP2, Q8WUP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.