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Protein

Golgi reassembly-stacking protein 1

Gene

Gorasp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes.By similarity

GO - Biological processi

  • Golgi organization Source: UniProtKB
  • negative regulation of dendrite morphogenesis Source: UniProtKB
  • protein N-linked glycosylation Source: MGI
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 1
Alternative name(s):
Golgi peripheral membrane protein p65
Golgi reassembly-stacking protein of 65 kDa
Short name:
GRASP65
Gene namesi
Name:Gorasp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1921748. Gorasp1.

Subcellular locationi

  • Golgi apparatuscis-Golgi network membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Undergoes rapid exchange with the cytosol.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • Golgi apparatus Source: MGI
  • membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCurated
Chaini2 – 446445Golgi reassembly-stacking protein 1PRO_0000087571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineCurated
Modified residuei216 – 2161PhosphothreonineCombined sources
Modified residuei220 – 2201PhosphothreonineCombined sources
Modified residuei224 – 2241PhosphothreonineBy similarity
Modified residuei365 – 3651PhosphoserineCombined sources
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking (By similarity).By similarity
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ91X51.
MaxQBiQ91X51.
PaxDbiQ91X51.
PRIDEiQ91X51.

PTM databases

iPTMnetiQ91X51.
PhosphoSiteiQ91X51.

Expressioni

Gene expression databases

BgeeiQ91X51.
CleanExiMM_GORASP1.
ExpressionAtlasiQ91X51. baseline and differential.
GenevisibleiQ91X51. MM.

Interactioni

Subunit structurei

Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115/USO1. Interacts with TMED2 and TMED3.By similarity

Protein-protein interaction databases

BioGridi216799. 22 interactions.
IntActiQ91X51. 26 interactions.
STRINGi10090.ENSMUSP00000035099.

Structurei

3D structure databases

ProteinModelPortaliQ91X51.
SMRiQ91X51. Positions 12-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7470PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 20113Essential for the interaction with GOLGA2/GM130By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi298 – 3014Poly-Pro
Compositional biasi314 – 35542Ser-richAdd
BLAST
Compositional biasi351 – 3555Poly-Ser

Sequence similaritiesi

Belongs to the GORASP family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
GeneTreeiENSGT00390000008686.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiQ91X51.
OMAiSGPEDVC.
OrthoDBiEOG7BS4B1.
PhylomeDBiQ91X51.
TreeFamiTF314053.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91X51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGASSEQP AGGEGFHLHG VQENSPAQQA GLEPYFDFII TIGHSRLNKE
60 70 80 90 100
NDTLKALLKA NVEKPVKLEV FNMKTMKVRE VEVVPSNMWG GQGLLGASVR
110 120 130 140 150
FCSFRRASEH VWHVLDVEPS SPAALAGLCP YTDYIVGSDQ ILQESEDFFT
160 170 180 190 200
LIESHEGKPL KLMVYNSESD SCREVTVTPN AAWGGEGSLG CGIGYGYLHR
210 220 230 240 250
IPTQPSSQHK KPPGATPPGT PATTSQLTAF PLGAPPPWPI PQDSSGPELG
260 270 280 290 300
SRQSDFMEAL PQVPGSFMEG QLLGPGSPSH GAADCGGCLR AMEIPLQPPP
310 320 330 340 350
PVQRVMDPGF LDVSGMSLLD SSNISVCPSL SSSTVLTSTA VSVSGPEDIG
360 370 380 390 400
SSSSSHERGG EATWSGSEFE ISFPDSPGAQ AQADHLPRLT LPDGLTSAAS
410 420 430 440
PEEGLSAELL EAQTEEPADT ASLDCRAETE GRASQAQATP DPEPGL
Length:446
Mass (Da):46,882
Last modified:January 23, 2007 - v3
Checksum:i05A59D996B9AD56B
GO

Sequence cautioni

The sequence BAB30676.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012251 mRNA. Translation: AAH12251.1.
AK017293 mRNA. Translation: BAB30676.1. Different initiation.
CCDSiCCDS23619.1.
RefSeqiNP_083252.1. NM_028976.2.
UniGeneiMm.104789.

Genome annotation databases

EnsembliENSMUST00000035099; ENSMUSP00000035099; ENSMUSG00000032513.
GeneIDi74498.
KEGGimmu:74498.
UCSCiuc009sbq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012251 mRNA. Translation: AAH12251.1.
AK017293 mRNA. Translation: BAB30676.1. Different initiation.
CCDSiCCDS23619.1.
RefSeqiNP_083252.1. NM_028976.2.
UniGeneiMm.104789.

3D structure databases

ProteinModelPortaliQ91X51.
SMRiQ91X51. Positions 12-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216799. 22 interactions.
IntActiQ91X51. 26 interactions.
STRINGi10090.ENSMUSP00000035099.

PTM databases

iPTMnetiQ91X51.
PhosphoSiteiQ91X51.

Proteomic databases

EPDiQ91X51.
MaxQBiQ91X51.
PaxDbiQ91X51.
PRIDEiQ91X51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035099; ENSMUSP00000035099; ENSMUSG00000032513.
GeneIDi74498.
KEGGimmu:74498.
UCSCiuc009sbq.1. mouse.

Organism-specific databases

CTDi64689.
MGIiMGI:1921748. Gorasp1.

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
GeneTreeiENSGT00390000008686.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiQ91X51.
OMAiSGPEDVC.
OrthoDBiEOG7BS4B1.
PhylomeDBiQ91X51.
TreeFamiTF314053.

Enzyme and pathway databases

ReactomeiR-MMU-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

PROiQ91X51.
SOURCEiSearch...

Gene expression databases

BgeeiQ91X51.
CleanExiMM_GORASP1.
ExpressionAtlasiQ91X51. baseline and differential.
GenevisibleiQ91X51. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 312-446.
    Strain: C57BL/6J.
    Tissue: Head.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216; THR-220; SER-365 AND SER-376, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGORS1_MOUSE
AccessioniPrimary (citable) accession number: Q91X51
Secondary accession number(s): Q9D3L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.