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Protein

Glucokinase regulatory protein

Gene

Gckr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531Fructose 1-phosphateBy similarity
Binding sitei348 – 3481Fructose 1-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • cellular glucose homeostasis Source: MGI
  • negative regulation of glucokinase activity Source: UniProtKB
  • positive regulation of glucokinase activity Source: BHF-UCL
  • protein import into nucleus, translocation Source: MGI
  • protein localization to nucleus Source: MGI
  • response to fructose Source: MGI
  • triglyceride homeostasis Source: MGI
  • urate metabolic process Source: MGI
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase regulatory protein
Short name:
Glucokinase regulator
Gene namesi
Name:Gckr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1096345. Gckr.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Under low glucose concentrations, GCK associates with GKRP and the inactive complex is recruited to the hepatocyte nucleus.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Ensembl
  • mitochondrion Source: Ensembl
  • nucleoplasm Source: UniProtKB
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3232700.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Glucokinase regulatory proteinPRO_0000248832Add
BLAST

Proteomic databases

EPDiQ91X44.
MaxQBiQ91X44.
PaxDbiQ91X44.
PRIDEiQ91X44.

PTM databases

iPTMnetiQ91X44.
PhosphoSiteiQ91X44.

Expressioni

Gene expression databases

BgeeiQ91X44.
CleanExiMM_GCKR.
ExpressionAtlasiQ91X44. baseline and differential.
GenevisibleiQ91X44. MM.

Interactioni

Subunit structurei

Interacts (fructose 6-phosphate bound form) with GCK.By similarity

Protein-protein interaction databases

IntActiQ91X44. 1 interaction.
MINTiMINT-1869582.
STRINGi10090.ENSMUSP00000072084.

Chemistry

BindingDBiQ91X44.

Structurei

3D structure databases

ProteinModelPortaliQ91X44.
SMRiQ91X44. Positions 7-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 286197SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini320 – 476157SIS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 1102Fructose 1-phosphate/Fructose 6-phosphate bindingBy similarity
Regioni179 – 1813Fructose 1-phosphate/Fructose 6-phosphate bindingBy similarity
Regioni199 – 2002Important for interaction with GCKBy similarity
Regioni463 – 4653Essential for interaction with GCKBy similarity

Domaini

Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.By similarity

Sequence similaritiesi

Belongs to the GCKR family.Curated
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOGENOMiHOG000031501.
HOVERGENiHBG005818.
InParanoidiQ91X44.
OMAiAGQMDYC.
OrthoDBiEOG7HXCRC.
PhylomeDBiQ91X44.
TreeFamiTF329177.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91X44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTKRYQHV IETPEPGEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV
60 70 80 90 100
QLLGQCDAEI FQEEGQIMPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG
110 120 130 140 150
LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE
160 170 180 190 200
RTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV
210 220 230 240 250
FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG
260 270 280 290 300
PEGLSGSSRM KGGSATKILL ETLLLAAHKT VDQGVVSSQR CLLEILRTFE
310 320 330 340 350
RAHQVTYSQS SKIATLTKQV GISLEKKGHV HLVGWQTLGI IAIMDGVECI
360 370 380 390 400
HTFGADFRDI RGFLIGDHND MFNQKDELSN QGPQFTFSQD DFLTSVLPSL
410 420 430 440 450
TEIDTVVFIF TLDDNLAEVQ ALAERVREKS WNIQALVHST VGQSLPAPLK
460 470 480 490 500
KLFPSLISIT WPLLFFDYEG SYVQKFQREL STKWVLNTRF SGQSKARCIE
510 520 530 540 550
SLLQVIHFPQ PLSNDVRAAP ISCHVQVAHE KEKVIPTALL SLLLRCSITE
560 570 580
AKERLAAASS VCEVVRSALS GPGQKRSIQA FGDPVVP
Length:587
Mass (Da):64,679
Last modified:January 23, 2007 - v3
Checksum:i54319C68A136952A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012412 mRNA. Translation: AAH12412.1.
CCDSiCCDS19182.1.
RefSeqiNP_659158.1. NM_144909.1.
UniGeneiMm.100043.

Genome annotation databases

EnsembliENSMUST00000072228; ENSMUSP00000072084; ENSMUSG00000059434.
GeneIDi231103.
KEGGimmu:231103.
UCSCiuc008wyf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012412 mRNA. Translation: AAH12412.1.
CCDSiCCDS19182.1.
RefSeqiNP_659158.1. NM_144909.1.
UniGeneiMm.100043.

3D structure databases

ProteinModelPortaliQ91X44.
SMRiQ91X44. Positions 7-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91X44. 1 interaction.
MINTiMINT-1869582.
STRINGi10090.ENSMUSP00000072084.

Chemistry

BindingDBiQ91X44.
ChEMBLiCHEMBL3232700.

PTM databases

iPTMnetiQ91X44.
PhosphoSiteiQ91X44.

Proteomic databases

EPDiQ91X44.
MaxQBiQ91X44.
PaxDbiQ91X44.
PRIDEiQ91X44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072228; ENSMUSP00000072084; ENSMUSG00000059434.
GeneIDi231103.
KEGGimmu:231103.
UCSCiuc008wyf.1. mouse.

Organism-specific databases

CTDi2646.
MGIiMGI:1096345. Gckr.

Phylogenomic databases

eggNOGiENOG410IHMK. Eukaryota.
ENOG4111GRB. LUCA.
GeneTreeiENSGT00390000005345.
HOGENOMiHOG000031501.
HOVERGENiHBG005818.
InParanoidiQ91X44.
OMAiAGQMDYC.
OrthoDBiEOG7HXCRC.
PhylomeDBiQ91X44.
TreeFamiTF329177.

Enzyme and pathway databases

ReactomeiR-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.

Miscellaneous databases

NextBioi380405.
PROiQ91X44.
SOURCEiSearch...

Gene expression databases

BgeeiQ91X44.
CleanExiMM_GCKR.
ExpressionAtlasiQ91X44. baseline and differential.
GenevisibleiQ91X44. MM.

Family and domain databases

InterProiIPR005486. Glucokinase_regulatory_CS.
IPR001347. SIS.
[Graphical view]
PROSITEiPS01272. GCKR. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGCKR_MOUSE
AccessioniPrimary (citable) accession number: Q91X44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.