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Protein

Bile acid-CoA:amino acid N-acyltransferase

Gene

Baat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.By similarity

Miscellaneous

Mouse BAAT seems to be selective for taurine conjugation of cholyl CoA. In mouse only taurine-conjugated bile acids are found in bile.

Catalytic activityi

Choloyl-CoA + glycine = CoA + glycocholate.1 Publication
Palmitoyl-CoA + H2O = CoA + palmitate.By similarity

Kineticsi

  1. KM=1.9 mM for taurine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei235Charge relay systemBy similarity1
    Active sitei328Charge relay systemBy similarity1
    Active sitei362Charge relay systemBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Hydrolase, Serine esterase, Transferase
    Biological processFatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiR-MMU-159418 Recycling of bile acids and salts
    R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
    R-MMU-9033241 Peroxisomal protein import
    SABIO-RKQ91X34

    Protein family/group databases

    ESTHERimouse-BAAT Acyl-CoA_Thioesterase
    MEROPSiS09.A50

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bile acid-CoA:amino acid N-acyltransferase (EC:2.3.1.651 Publication)
    Short name:
    BACAT
    Short name:
    BAT
    Alternative name(s):
    Glycine N-choloyltransferase
    Long-chain fatty-acyl-CoA hydrolase (EC:3.1.2.2By similarity)
    Gene namesi
    Name:Baat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 4

    Organism-specific databases

    MGIiMGI:106642 Baat

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002021601 – 420Bile acid-CoA:amino acid N-acyltransferaseAdd BLAST420

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei40N6-succinyllysineCombined sources1
    Modified residuei125PhosphoserineBy similarity1
    Modified residuei346N6-succinyllysineCombined sources1
    Modified residuei350N6-succinyllysineCombined sources1
    Modified residuei409N6-succinyllysineCombined sources1
    Modified residuei418PhosphoserineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ91X34
    PaxDbiQ91X34
    PeptideAtlasiQ91X34
    PRIDEiQ91X34

    PTM databases

    iPTMnetiQ91X34
    PhosphoSitePlusiQ91X34
    SwissPalmiQ91X34

    Expressioni

    Tissue specificityi

    Highly expressed in liver, kidney, gallbladder, proximal intestine and distal intestine. Weakly expressed in adrenal gland, lung, brain and muscle.2 Publications

    Gene expression databases

    BgeeiENSMUSG00000039653
    GenevisibleiQ91X34 MM

    Interactioni

    Subunit structurei

    Monomer.By similarity

    GO - Molecular functioni

    Protein-protein interaction databases

    IntActiQ91X34, 3 interactors
    MINTiQ91X34
    STRINGi10090.ENSMUSP00000041983

    Structurei

    3D structure databases

    ProteinModelPortaliQ91X34
    SMRiQ91X34
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the C/M/P thioester hydrolase family.Curated

    Phylogenomic databases

    eggNOGiENOG410II3X Eukaryota
    COG1073 LUCA
    GeneTreeiENSGT00390000001046
    HOGENOMiHOG000116219
    HOVERGENiHBG000331
    InParanoidiQ91X34
    KOiK00659
    OMAiIPQVYRG
    OrthoDBiEOG091G08KU
    PhylomeDBiQ91X34
    TreeFamiTF314911

    Family and domain databases

    Gene3Di3.40.50.1820, 1 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR016662 Acyl-CoA_thioEstase_long-chain
    IPR014940 BAAT_C
    IPR006862 Thio_Ohase/aa_AcTrfase
    PfamiView protein in Pfam
    PF08840 BAAT_C, 1 hit
    PF04775 Bile_Hydr_Trans, 1 hit
    PIRSFiPIRSF016521 Acyl-CoA_hydro, 1 hit
    SUPFAMiSSF53474 SSF53474, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q91X34-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKLTAVPLS ALVDEPVHIQ VTGLAPFQVV CLQASLKDEK GNLFSSQAFY
    60 70 80 90 100
    RASEVGEVDL EHDPSLGGDY MGVHPMGLFW SLKPEKLLGR LIKRDVMNSP
    110 120 130 140 150
    YQIHIKACHP YFPLQDIVVS PPLDSLTLER WYVAPGVKRI QVKESRIRGA
    160 170 180 190 200
    LFLPPGEGPF PGVIDLFGGA GGLMEFRASL LASRGFATLA LAYWNYDDLP
    210 220 230 240 250
    SRLEKVDLEY FEEGVEFLLR HPKVLGPGVG ILSVCIGAEI GLSMAINLKQ
    260 270 280 290 300
    IRATVLINGP NFVSQSPHVY HGQVYPPVPS NEEFVVTNAL GLVEFYRTFQ
    310 320 330 340 350
    ETADKDSKYC FPIEKAHGHF LFVVGEDDKN LNSKVHANQA IAQLMKNGKK
    360 370 380 390 400
    NWTLLSYPGA GHLIEPPYTP LCQASRMPIL IPSLSWGGEV IPHAAAQEHS
    410 420
    WKEIQKFLKQ HLLPDLSSQL
    Length:420
    Mass (Da):46,482
    Last modified:December 1, 2001 - v1
    Checksum:iAABC6DB573C5EF50
    GO

    Sequence cautioni

    The sequence AAB58325 differs from that shown. Reason: Frameshift at positions 40 and 44.Curated
    The sequence BAC25534 differs from that shown. Reason: Frameshift at position 63.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti97M → I in AAB58325 (PubMed:9215542).Curated1
    Sequence conflicti117I → L in AAB58325 (PubMed:9215542).Curated1
    Sequence conflicti268H → Q in BAC25534 (PubMed:16141072).Curated1
    Sequence conflicti385S → R in BAC25534 (PubMed:16141072).Curated1
    Sequence conflicti394A → SQ in AAB58325 (PubMed:9215542).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U95215 mRNA Translation: AAB58325.1 Frameshift.
    AK017923 mRNA Translation: BAC25534.1 Frameshift.
    AL772310 Genomic DNA Translation: CAM21769.1
    CH466565 Genomic DNA Translation: EDL02319.1
    BC012683 mRNA No translation available.
    CCDSiCCDS18173.1
    RefSeqiNP_031545.2, NM_007519.3
    UniGeneiMm.2859

    Genome annotation databases

    EnsembliENSMUST00000043056; ENSMUSP00000041983; ENSMUSG00000039653
    ENSMUST00000166036; ENSMUSP00000129603; ENSMUSG00000039653
    GeneIDi12012
    KEGGimmu:12012
    UCSCiuc008svt.2 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiBAAT_MOUSE
    AccessioniPrimary (citable) accession number: Q91X34
    Secondary accession number(s): A2AKK7, O08833, Q8C1I3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: December 1, 2001
    Last modified: May 23, 2018
    This is version 126 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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