Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Patatin-like phospholipase domain-containing protein 3

Gene

Pnpla3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities.By similarity

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Pathwayi: triacylglycerol degradation

This protein is involved in the pathway triacylglycerol degradation, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol degradation and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47NucleophilePROSITE-ProRule annotation1
Active sitei166Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • diolein transacylation activity Source: MGI
  • long-chain fatty acyl-CoA binding Source: BHF-UCL
  • lysophosphatidic acid acyltransferase activity Source: BHF-UCL
  • lysophosphatidic acid binding Source: BHF-UCL
  • mono-olein transacylation activity Source: MGI
  • phospholipase A2 activity Source: UniProtKB
  • triglyceride lipase activity Source: BHF-UCL

GO - Biological processi

  • glycerophospholipid metabolic process Source: BHF-UCL
  • lipid homeostasis Source: GO_Central
  • lipid particle organization Source: MGI
  • long-chain fatty acid metabolic process Source: BHF-UCL
  • phosphatidic acid biosynthetic process Source: BHF-UCL
  • response to dietary excess Source: BHF-UCL
  • triglyceride acyl-chain remodeling Source: MGI
  • triglyceride biosynthetic process Source: BHF-UCL
  • triglyceride catabolic process Source: BHF-UCL

Keywordsi

Molecular functionAcyltransferase, Hydrolase, Transferase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00256.

Chemistry databases

SwissLipidsiSLP:000000281.

Names & Taxonomyi

Protein namesi
Recommended name:
Patatin-like phospholipase domain-containing protein 3 (EC:3.1.1.3)
Alternative name(s):
Acylglycerol O-acyltransferase (EC:2.3.1.-)
Adiponutrin
Calcium-independent phospholipase A2-epsilon
Short name:
iPLA2-epsilon
Gene namesi
Name:Pnpla3
Synonyms:Adpn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2151796. Pnpla3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 42CytoplasmicSequence analysisAdd BLAST42
Transmembranei43 – 63Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini64 – 413LumenalSequence analysisAdd BLAST350

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644591 – 413Patatin-like phospholipase domain-containing protein 3Add BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ91WW7.
PRIDEiQ91WW7.

PTM databases

PhosphoSitePlusiQ91WW7.

Expressioni

Tissue specificityi

Restricted to adipose tissue. Expressed in inguinal and epididymal white adipose tissues and in interscapular brown adipose tissue.1 Publication

Inductioni

Down-regulated after starvation and up-regulated after refeeding.

Gene expression databases

CleanExiMM_PNPLA3.

Interactioni

Protein-protein interaction databases

MINTiMINT-4997871.
STRINGi10090.ENSMUSP00000043826.

Structurei

3D structure databases

ProteinModelPortaliQ91WW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 179PNPLAPROSITE-ProRule annotationAdd BLAST170

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 19GXGXXGPROSITE-ProRule annotation6
Motifi45 – 49GXSXGPROSITE-ProRule annotation5
Motifi166 – 168DGA/GPROSITE-ProRule annotation3

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3773. Eukaryota.
ENOG410XSQS. LUCA.
HOGENOMiHOG000007467.
HOVERGENiHBG007046.
InParanoidiQ91WW7.
KOiK13534.
PhylomeDBiQ91WW7.

Family and domain databases

InterProiView protein in InterPro
IPR016035. Acyl_Trfase/lysoPLipase.
IPR033562. PLPL.
IPR002641. PNPLA_dom.
PANTHERiPTHR12406. PTHR12406. 1 hit.
PfamiView protein in Pfam
PF01734. Patatin. 1 hit.
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiView protein in PROSITE
PS51635. PNPLA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91WW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDPERRWSL SFAGCGFLGF YHVGATLCLS ERAPHLLRDA RTFFGCSAGA
60 70 80 90 100
LHAVTFVCSL PLGRIMEILM DLVRKARSRN IGTLHPFFNI NKCIRDGLQE
110 120 130 140 150
SLPDNVHQVI SGKVHISLTR VSDGENVLVS EFHSKDEVVD ALVCSCFIPL
160 170 180 190 200
FSGLIPPSFR GERYVDGGVS DNVPVLDAKT TITVSPFYGE HDICPKVKST
210 220 230 240 250
NFFHVNITNL SLRLCTGNLQ LLTRALFPSD VKVMGELCYQ GYLDAFRFLE
260 270 280 290 300
ENGICNGPQR SLSLSLVAPE ACLENGKLVG DKVPVSLCFT DENIWETLSP
310 320 330 340 350
ELSTALSEAI KDREGYLSKV CNLLPVRILS YIMLPCSLPV ESAIAAVHRL
360 370 380 390 400
VTWLPDIQDD IQWLQWATSQ VCARMTMCLL PSTRSRASKD DHRMLKHGHH
410
PSPHKPQGNS AGL
Length:413
Mass (Da):45,772
Last modified:December 1, 2001 - v1
Checksum:i7D697E81F66A83C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037763 mRNA. Translation: AAK68636.1.
BC028792 mRNA. Translation: AAH28792.1.
RefSeqiNP_473429.2. NM_054088.3.
UniGeneiMm.390817.

Genome annotation databases

GeneIDi116939.
KEGGimmu:116939.
UCSCiuc007xbw.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPLPL3_MOUSE
AccessioniPrimary (citable) accession number: Q91WW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways