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Protein

Arsenite methyltransferase

Gene

As3mt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate.
S-adenosyl-L-methionine + methylarsonite = S-adenosyl-L-homocysteine + dimethylarsinate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.137. 3474.
ReactomeiR-MMU-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite methyltransferase (EC:2.1.1.137)
Alternative name(s):
Methylarsonite methyltransferase
S-adenosyl-L-methionine:arsenic(III) methyltransferase
Gene namesi
Name:As3mt
Synonyms:Cyt19
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1929882. As3mt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Arsenite methyltransferasePRO_0000204448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei336 – 3361PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91WU5.
MaxQBiQ91WU5.
PaxDbiQ91WU5.
PRIDEiQ91WU5.

PTM databases

iPTMnetiQ91WU5.
PhosphoSiteiQ91WU5.

Expressioni

Gene expression databases

BgeeiQ91WU5.
CleanExiMM_AS3MT.
GenevisibleiQ91WU5. MM.

Interactioni

Protein-protein interaction databases

BioGridi208264. 1 interaction.
STRINGi10090.ENSMUSP00000003655.

Structurei

3D structure databases

ProteinModelPortaliQ91WU5.
SMRiQ91WU5. Positions 40-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410IFW3. Eukaryota.
ENOG410XSKB. LUCA.
GeneTreeiENSGT00390000001742.
HOGENOMiHOG000229966.
HOVERGENiHBG050585.
InParanoidiQ91WU5.
KOiK07755.
OMAiVVPECLE.
OrthoDBiEOG7V49ZQ.
TreeFamiTF343797.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF883. PTHR10108:SF883. 1 hit.
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequencei

Sequence statusi: Complete.

Q91WU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASRDADEI HKDVQNYYGN VLKTSADLQT NACVTRAKPV PSYIRESLQN
60 70 80 90 100
VHEDVSSRYY GCGLTVPERL ENCRILDLGS GSGRDCYVLS QLVGEKGHVT
110 120 130 140 150
GIDMTEVQVE VAKTYLEHHM EKFGFQAPNV TFLHGRIEKL AEAGIQSESY
160 170 180 190 200
DIVISNCVIN LVPDKQQVLQ EVYRVLKHGG ELYFSDVYAS LEVPEDIKSH
210 220 230 240 250
KVLWGECLGG ALYWKDLAII AQKIGFCPPR LVTADIITVE NKELEGVLGD
260 270 280 290 300
CRFVSATFRL FKLPKTEPAE RCRVVYNGGI KGHEKELIFD ANFTFKEGEA
310 320 330 340 350
VAVDEETAAV LKNSRFAPDF LFTPVDASLP APQGRSELET KVLIRDPFKL
360 370
AEDSDKMKPR HAPEGTGGCC GKRKNC
Length:376
Mass (Da):41,794
Last modified:July 27, 2011 - v2
Checksum:i9421A663FCC825E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061E → K in AAI45664 (PubMed:15489334).Curated
Sequence conflicti151 – 1511D → G in AAF00618 (Ref. 1) Curated
Sequence conflicti335 – 34612Missing in AAF00618 (Ref. 1) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166383 mRNA. Translation: AAF00618.1.
AC161865 Genomic DNA. No translation available.
BC145663 mRNA. Translation: AAI45664.1.
BC013468 mRNA. No translation available.
CCDSiCCDS50458.1.
PIRiT44795.
RefSeqiNP_065602.2. NM_020577.2.
UniGeneiMm.28566.

Genome annotation databases

EnsembliENSMUST00000003655; ENSMUSP00000003655; ENSMUSG00000003559.
GeneIDi57344.
KEGGimmu:57344.
UCSCiuc008huc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166383 mRNA. Translation: AAF00618.1.
AC161865 Genomic DNA. No translation available.
BC145663 mRNA. Translation: AAI45664.1.
BC013468 mRNA. No translation available.
CCDSiCCDS50458.1.
PIRiT44795.
RefSeqiNP_065602.2. NM_020577.2.
UniGeneiMm.28566.

3D structure databases

ProteinModelPortaliQ91WU5.
SMRiQ91WU5. Positions 40-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208264. 1 interaction.
STRINGi10090.ENSMUSP00000003655.

PTM databases

iPTMnetiQ91WU5.
PhosphoSiteiQ91WU5.

Proteomic databases

EPDiQ91WU5.
MaxQBiQ91WU5.
PaxDbiQ91WU5.
PRIDEiQ91WU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003655; ENSMUSP00000003655; ENSMUSG00000003559.
GeneIDi57344.
KEGGimmu:57344.
UCSCiuc008huc.1. mouse.

Organism-specific databases

CTDi57412.
MGIiMGI:1929882. As3mt.

Phylogenomic databases

eggNOGiENOG410IFW3. Eukaryota.
ENOG410XSKB. LUCA.
GeneTreeiENSGT00390000001742.
HOGENOMiHOG000229966.
HOVERGENiHBG050585.
InParanoidiQ91WU5.
KOiK07755.
OMAiVVPECLE.
OrthoDBiEOG7V49ZQ.
TreeFamiTF343797.

Enzyme and pathway databases

BRENDAi2.1.1.137. 3474.
ReactomeiR-MMU-156581. Methylation.

Miscellaneous databases

PROiQ91WU5.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WU5.
CleanExiMM_AS3MT.
GenevisibleiQ91WU5. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF883. PTHR10108:SF883. 1 hit.
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel hematopoietic stem cell regulatory genes."
    Phillips R.L., Ernst R.E., Dosil M., Wesley C.K., Moore K.A., Kingsley P.D., Sykes S., Palis J., Lemischka I.R.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain and Colon.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiAS3MT_MOUSE
AccessioniPrimary (citable) accession number: Q91WU5
Secondary accession number(s): A6H5W4, E9QLU6, Q9QZT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.