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Q91WU2 (S22A7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 7
Alternative name(s):
Organic anion transporter 2
Short name=mOAT2
Gene names
Name:Slc22a7
Synonyms:Oat2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates sodium-independent multispecific organic anion transport. High affinity transport of glutarate and prostaglandin E2 in a sodium-independent manner. Mediates also the uptake of alpha-ketoglutarate, p-aminohippuric acid, methotrexate, ochratoxin A, valproate, allopurinol and bumetanide. Ref.4

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein. Note: Apical side of the renal tubule By similarity.

Tissue specificity

Abundant expression in male and female kidney and also in female liver. Ref.1

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=15.8 µM for glutarate Ref.1 Ref.4

KM=5.2 nM for prostaglandin E2

KM=9.12 µM for bumetanide

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q91WU2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q91WU2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: Missing.
     125-131: SSTIATE → MGESSET

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 540540Solute carrier family 22 member 7
PRO_0000317482

Regions

Transmembrane21 – 4121Helical; Potential
Transmembrane144 – 16421Helical; Potential
Transmembrane172 – 19221Helical; Potential
Transmembrane202 – 22221Helical; Potential
Transmembrane232 – 25221Helical; Potential
Transmembrane257 – 27721Helical; Potential
Transmembrane344 – 36421Helical; Potential
Transmembrane378 – 39821Helical; Potential
Transmembrane402 – 42221Helical; Potential
Transmembrane429 – 44921Helical; Potential
Transmembrane462 – 48423Helical; Potential
Transmembrane488 – 51023Helical; Potential

Natural variations

Alternative sequence1 – 124124Missing in isoform 2.
VSP_030995
Alternative sequence125 – 1317SSTIATE → MGESSET in isoform 2.
VSP_030996

Experimental info

Sequence conflict461A → T in BAC02736. Ref.1
Sequence conflict821R → H in BAC02736. Ref.1
Sequence conflict851Y → C in BAC38659. Ref.2
Sequence conflict1371E → Q in BAC38659. Ref.2
Sequence conflict1371E → Q in BAE25625. Ref.2
Sequence conflict1931Y → N in BAC38659. Ref.2
Sequence conflict2551W → S in AAH26598. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 2A5AA5A5E0F30BAE

FASTA54059,614
        10         20         30         40         50         60 
MGFEELLHKV GGFGPFQLRN LVLLALPRFL LPMHFLLPIF MAAVPAHHCA LPDAPANLSH 

        70         80         90        100        110        120 
QDLWLKTHLP RETDGSFSSC LRFAYPQALP NVTLGTEVYN SGEPEGEPLT VPCSQGWEYD 

       130        140        150        160        170        180 
RSEFSSTIAT EWDLVCEQRG LNKVTSTCFF IGVLLGAVVY GYLSDRFGRR RLLLVAYVST 

       190        200        210        220        230        240 
LALGLMSAAS VNYIMFVTTR MLTGSALAGF TIIVLPLELE WLDVEHRTVA GVISTTFWTG 

       250        260        270        280        290        300 
GVLLLTLVGY LIRSWRWLLL AATLPCVPGI ISIWWVPESA RWLLTQGRVE EAKKYLSICA 

       310        320        330        340        350        360 
KLNGRPISED SLSQEALNKV ITMERVSQRP SYLDLFRTSQ LRHVSLCCMM MWFGVNFSYY 

       370        380        390        400        410        420 
GLTLDASGLG LTVYQTQLLF GAVEVPSKIT VFFLVRLVGR RLTEAGMLLA TALTFGISLL 

       430        440        450        460        470        480 
VSSDTKSWIT ALVVIGKAFS EAAFTTAYLF TSELYPTVLR QTGMGFTALI GRLGASLAPL 

       490        500        510        520        530        540 
VVLLDGVWLL LPKLAYGGIS FLAACTVLLL PETKKAQLPE TIQDVERKGR KIDRSGTELA 

« Hide

Isoform 2 [UniParc].

Checksum: 2A6DDB3800FC3136
Show »

FASTA41645,803

References

« Hide 'large scale' references
[1]"Isolation, characterization and differential gene expression of multispecific organic anion transporter 2 in mice."
Kobayashi Y., Ohshiro N., Shibusawa A., Sasaki T., Tokuyama S., Sekine T., Endou H., Yamamoto T.
Mol. Pharmacol. 62:7-14(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
Tissue: Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Kidney and Liver.
[4]"Mouse organic anion transporter 2 and 3 (mOAT2/3[Slc22a7/8]) mediates the renal transport of bumetanide."
Kobayashi Y., Ohbayashi M., Kohyama N., Yamamoto T.
Eur. J. Pharmacol. 524:44-48(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB069965 mRNA. Translation: BAC02736.1.
AK082865 mRNA. Translation: BAC38659.1.
AK143949 mRNA. Translation: BAE25625.1.
BC013474 mRNA. Translation: AAH13474.1.
BC024119 mRNA. Translation: AAH24119.1.
BC025813 mRNA. Translation: AAH25813.1.
BC026598 mRNA. Translation: AAH26598.1.
BC026597 mRNA. Translation: AAH26597.1.
CCDSCCDS28829.1. [Q91WU2-1]
RefSeqNP_659105.2. NM_144856.2.
UniGeneMm.387538.

3D structure databases

ProteinModelPortalQ91WU2.
SMRQ91WU2. Positions 144-514.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2073716.

PTM databases

PhosphoSiteQ91WU2.

Proteomic databases

PaxDbQ91WU2.
PRIDEQ91WU2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087012; ENSMUSP00000084234; ENSMUSG00000067144.
GeneID108114.
KEGGmmu:108114.
UCSCuc008csw.2. mouse. [Q91WU2-1]

Organism-specific databases

CTD10864.
MGIMGI:1859559. Slc22a7.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00750000117345.
HOGENOMHOG000234569.
HOVERGENHBG108433.
InParanoidQ91WU2.
KOK08204.
OrthoDBEOG7C8GH9.
PhylomeDBQ91WU2.
TreeFamTF315847.

Gene expression databases

BgeeQ91WU2.
GenevestigatorQ91WU2.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00898. 2A0119. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio360092.
PROQ91WU2.
SOURCESearch...

Entry information

Entry nameS22A7_MOUSE
AccessionPrimary (citable) accession number: Q91WU2
Secondary accession number(s): Q3UNX2 expand/collapse secondary AC list , Q8BUQ9, Q8K4S9, Q8R0M7, Q8R125
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot