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Protein

Solute carrier family 22 member 7

Gene

Slc22a7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates sodium-independent multispecific organic anion transport. High affinity transport of glutarate and prostaglandin E2 in a sodium-independent manner. Mediates also the uptake of alpha-ketoglutarate, p-aminohippuric acid, methotrexate, ochratoxin A, valproate, allopurinol and bumetanide.1 Publication

Kineticsi

  1. KM=15.8 µM for glutarate2 Publications
  2. KM=5.2 nM for prostaglandin E22 Publications
  3. KM=9.12 µM for bumetanide2 Publications

    GO - Molecular functioni

    • organic anion transmembrane transporter activity Source: MGI

    GO - Biological processi

    • organic anion transport Source: MGI
    • response to stilbenoid Source: UniProtKB
    Complete GO annotation...

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_335065. Organic anion transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 7
    Alternative name(s):
    Organic anion transporter 2
    Short name:
    mOAT2
    Gene namesi
    Name:Slc22a7
    Synonyms:Oat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Unplaced

    Organism-specific databases

    MGIiMGI:1859559. Slc22a7.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei257 – 27721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei378 – 39821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei429 – 44921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei462 – 48423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei488 – 51023HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • basolateral plasma membrane Source: UniProtKB-SubCell
    • integral component of plasma membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540Solute carrier family 22 member 7PRO_0000317482Add
    BLAST

    Proteomic databases

    PaxDbiQ91WU2.
    PRIDEiQ91WU2.

    PTM databases

    PhosphoSiteiQ91WU2.

    Expressioni

    Tissue specificityi

    Abundant expression in male and female kidney and also in female liver.1 Publication

    Gene expression databases

    BgeeiQ91WU2.
    GenevestigatoriQ91WU2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91WU2.
    SMRiQ91WU2. Positions 144-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ91WU2.
    KOiK08204.
    OrthoDBiEOG7C8GH9.
    PhylomeDBiQ91WU2.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q91WU2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGFEELLHKV GGFGPFQLRN LVLLALPRFL LPMHFLLPIF MAAVPAHHCA
    60 70 80 90 100
    LPDAPANLSH QDLWLKTHLP RETDGSFSSC LRFAYPQALP NVTLGTEVYN
    110 120 130 140 150
    SGEPEGEPLT VPCSQGWEYD RSEFSSTIAT EWDLVCEQRG LNKVTSTCFF
    160 170 180 190 200
    IGVLLGAVVY GYLSDRFGRR RLLLVAYVST LALGLMSAAS VNYIMFVTTR
    210 220 230 240 250
    MLTGSALAGF TIIVLPLELE WLDVEHRTVA GVISTTFWTG GVLLLTLVGY
    260 270 280 290 300
    LIRSWRWLLL AATLPCVPGI ISIWWVPESA RWLLTQGRVE EAKKYLSICA
    310 320 330 340 350
    KLNGRPISED SLSQEALNKV ITMERVSQRP SYLDLFRTSQ LRHVSLCCMM
    360 370 380 390 400
    MWFGVNFSYY GLTLDASGLG LTVYQTQLLF GAVEVPSKIT VFFLVRLVGR
    410 420 430 440 450
    RLTEAGMLLA TALTFGISLL VSSDTKSWIT ALVVIGKAFS EAAFTTAYLF
    460 470 480 490 500
    TSELYPTVLR QTGMGFTALI GRLGASLAPL VVLLDGVWLL LPKLAYGGIS
    510 520 530 540
    FLAACTVLLL PETKKAQLPE TIQDVERKGR KIDRSGTELA
    Length:540
    Mass (Da):59,614
    Last modified:December 1, 2001 - v1
    Checksum:i2A5AA5A5E0F30BAE
    GO
    Isoform 2 (identifier: Q91WU2-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-124: Missing.
         125-131: SSTIATE → MGESSET

    Show »
    Length:416
    Mass (Da):45,803
    Checksum:i2A6DDB3800FC3136
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461A → T in BAC02736 (PubMed:12065749).Curated
    Sequence conflicti82 – 821R → H in BAC02736 (PubMed:12065749).Curated
    Sequence conflicti85 – 851Y → C in BAC38659 (PubMed:16141072).Curated
    Sequence conflicti137 – 1371E → Q in BAC38659 (PubMed:16141072).Curated
    Sequence conflicti137 – 1371E → Q in BAE25625 (PubMed:16141072).Curated
    Sequence conflicti193 – 1931Y → N in BAC38659 (PubMed:16141072).Curated
    Sequence conflicti255 – 2551W → S in AAH26598 (PubMed:15489334).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 124124Missing in isoform 2. 2 PublicationsVSP_030995Add
    BLAST
    Alternative sequencei125 – 1317SSTIATE → MGESSET in isoform 2. 2 PublicationsVSP_030996

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB069965 mRNA. Translation: BAC02736.1.
    AK082865 mRNA. Translation: BAC38659.1.
    AK143949 mRNA. Translation: BAE25625.1.
    BC013474 mRNA. Translation: AAH13474.1.
    BC024119 mRNA. Translation: AAH24119.1.
    BC025813 mRNA. Translation: AAH25813.1.
    BC026598 mRNA. Translation: AAH26598.1.
    BC026597 mRNA. Translation: AAH26597.1.
    CCDSiCCDS28829.1. [Q91WU2-1]
    RefSeqiNP_659105.2. NM_144856.2.
    UniGeneiMm.387538.

    Genome annotation databases

    GeneIDi108114.
    KEGGimmu:108114.
    UCSCiuc008csw.2. mouse. [Q91WU2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB069965 mRNA. Translation: BAC02736.1.
    AK082865 mRNA. Translation: BAC38659.1.
    AK143949 mRNA. Translation: BAE25625.1.
    BC013474 mRNA. Translation: AAH13474.1.
    BC024119 mRNA. Translation: AAH24119.1.
    BC025813 mRNA. Translation: AAH25813.1.
    BC026598 mRNA. Translation: AAH26598.1.
    BC026597 mRNA. Translation: AAH26597.1.
    CCDSiCCDS28829.1. [Q91WU2-1]
    RefSeqiNP_659105.2. NM_144856.2.
    UniGeneiMm.387538.

    3D structure databases

    ProteinModelPortaliQ91WU2.
    SMRiQ91WU2. Positions 144-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Chemistry

    ChEMBLiCHEMBL2073716.

    PTM databases

    PhosphoSiteiQ91WU2.

    Proteomic databases

    PaxDbiQ91WU2.
    PRIDEiQ91WU2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi108114.
    KEGGimmu:108114.
    UCSCiuc008csw.2. mouse. [Q91WU2-1]

    Organism-specific databases

    CTDi10864.
    MGIiMGI:1859559. Slc22a7.

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ91WU2.
    KOiK08204.
    OrthoDBiEOG7C8GH9.
    PhylomeDBiQ91WU2.
    TreeFamiTF315847.

    Enzyme and pathway databases

    ReactomeiREACT_335065. Organic anion transport.

    Miscellaneous databases

    NextBioi360092.
    PROiQ91WU2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ91WU2.
    GenevestigatoriQ91WU2.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Isolation, characterization and differential gene expression of multispecific organic anion transporter 2 in mice."
      Kobayashi Y., Ohshiro N., Shibusawa A., Sasaki T., Tokuyama S., Sekine T., Endou H., Yamamoto T.
      Mol. Pharmacol. 62:7-14(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Kidney and Liver.
    4. "Mouse organic anion transporter 2 and 3 (mOAT2/3[Slc22a7/8]) mediates the renal transport of bumetanide."
      Kobayashi Y., Ohbayashi M., Kohyama N., Yamamoto T.
      Eur. J. Pharmacol. 524:44-48(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiS22A7_MOUSE
    AccessioniPrimary (citable) accession number: Q91WU2
    Secondary accession number(s): Q3UNX2
    , Q8BUQ9, Q8K4S9, Q8R0M7, Q8R125
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: December 1, 2001
    Last modified: May 27, 2015
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.