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Q91WT9

- CBS_MOUSE

UniProt

Q91WT9 - CBS_MOUSE

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Protein

Cystathionine beta-synthase

Gene
Cbs
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury By similarity.

Catalytic activityi

L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactori

Pyridoxal phosphate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Iron (heme axial ligand) By similarity
Metal bindingi62 – 621Iron (heme axial ligand) By similarity
Binding sitei146 – 1461Pyridoxal phosphate By similarity
Binding sitei346 – 3461Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. cystathionine beta-synthase activity Source: MGI
  3. heme binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
  5. modified amino acid binding Source: Ensembl
  6. pyridoxal phosphate binding Source: Ensembl

GO - Biological processi

  1. blood vessel remodeling Source: MGI
  2. cartilage development involved in endochondral bone morphogenesis Source: MGI
  3. cellular response to hypoxia Source: Ensembl
  4. cerebellum morphogenesis Source: MGI
  5. cysteine biosynthetic process Source: MGI
  6. cysteine biosynthetic process from serine Source: InterPro
  7. cysteine biosynthetic process via cystathionine Source: InterPro
  8. endochondral ossification Source: MGI
  9. homocysteine catabolic process Source: Ensembl
  10. homocysteine metabolic process Source: MGI
  11. hydrogen sulfide biosynthetic process Source: UniProtKB
  12. L-cysteine catabolic process Source: Ensembl
  13. L-serine catabolic process Source: Ensembl
  14. maternal process involved in female pregnancy Source: MGI
  15. negative regulation of apoptotic process Source: Ensembl
  16. regulation of blood vessel size Source: MGI
  17. regulation of cGMP metabolic process Source: MGI
  18. regulation of JUN kinase activity Source: MGI
  19. response to folic acid Source: MGI
  20. superoxide metabolic process Source: MGI
  21. transsulfuration Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_198986. Cysteine formation from homocysteine.
UniPathwayiUPA00136; UER00201.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-synthase (EC:4.2.1.22)
Alternative name(s):
Beta-thionase
Serine sulfhydrase
Gene namesi
Name:Cbs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:88285. Cbs.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. nucleolus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 561560Cystathionine beta-synthasePRO_0000262592Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161N6-(pyridoxal phosphate)lysine By similarity
Cross-linki208 – 208Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Binds covalently to a heme group through a thiolate ligand By similarity.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ91WT9.
PaxDbiQ91WT9.
PRIDEiQ91WT9.

PTM databases

PhosphoSiteiQ91WT9.

Expressioni

Gene expression databases

ArrayExpressiQ91WT9.
BgeeiQ91WT9.
CleanExiMM_CBS.
GenevestigatoriQ91WT9.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

IntActiQ91WT9. 1 interaction.
MINTiMINT-4121506.
STRINGi10090.ENSMUSP00000066878.

Structurei

3D structure databases

ProteinModelPortaliQ91WT9.
SMRiQ91WT9. Positions 40-558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini414 – 47461CBSAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 2575Pyridoxal phosphate binding By similarity

Sequence similaritiesi

Contains 1 CBS domain.

Keywords - Domaini

CBS domain

Phylogenomic databases

eggNOGiCOG0031.
GeneTreeiENSGT00510000047027.
HOGENOMiHOG000217392.
HOVERGENiHBG000918.
InParanoidiQ91WT9.
KOiK01697.
OMAiEEIWTQT.
OrthoDBiEOG7J70F1.
PhylomeDBiQ91WT9.
TreeFamiTF300784.

Family and domain databases

InterProiIPR000644. CBS_dom.
IPR005857. Cysta_beta_synth.
IPR001216. P-phosphate_BS.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00291. PALP. 1 hit.
[Graphical view]
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01137. cysta_beta. 1 hit.
PROSITEiPS51371. CBS. 1 hit.
PS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q91WT9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPSGTSQCED GSAGGFQHLD MHSEKRQLEK GPSGDKDRVW IRPDTPSRCT    50
WQLGRAMADS PHYHTVLTKS PKILPDILRK IGNTPMVRIN KISKNAGLKC 100
ELLAKCEFFN AGGSVKDRIS LRMIEDAERA GNLKPGDTII EPTSGNTGIG 150
LALAAAVKGY RCIIVMPEKM SMEKVDVLRA LGAEIVRTPT NARFDSPESH 200
VGVAWRLKNE IPNSHILDQY RNASNPLAHY DDTAEEILQQ CDGKLDMLVA 250
SAGTGGTITG IARKLKEKCP GCKIIGVDPE GSILAEPEEL NQTEQTAYEV 300
EGIGYDFIPT VLDRAVVDKW FKSNDEDSFA FARMLIAQEG LLCGGSSGSA 350
MAVAVKAARE LQEGQRCVVI LPDSVRNYMS KFLSDKWMLQ KGFMKEELSV 400
KRPWWWRLRV QELSLSAPLT VLPTVTCEDT IAILREKGFD QAPVVNESGA 450
ILGMVTLGNM LSSLLAGKVR PSDEVCKVLY KQFKPIHLTD TLGTLSHILE 500
MDHFALVVHE QIQSRDQAWS GVVGGPTDCS NGMSSKQQMV FGVVTAIDLL 550
NFVAAREQTQ T 561
Length:561
Mass (Da):61,544
Last modified:January 23, 2007 - v3
Checksum:i072CF81AFE10372E
GO
Isoform 2 (identifier: Q91WT9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     514-528: SRDQAWSGVVGGPTD → Y

Show »
Length:547
Mass (Da):60,193
Checksum:i5DC0F6E3DAED8461
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei514 – 52815SRDQA…GGPTD → Y in isoform 2. VSP_021790Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK077669 mRNA. Translation: BAC36943.1.
BC013472 mRNA. Translation: AAH13472.1.
BC013480 mRNA. Translation: AAH13480.1.
BC026595 mRNA. Translation: AAH26595.1.
CCDSiCCDS28609.1. [Q91WT9-1]
CCDS28610.1. [Q91WT9-2]
RefSeqiNP_001258282.1. NM_001271353.1. [Q91WT9-2]
NP_659104.1. NM_144855.3. [Q91WT9-1]
NP_835742.1. NM_178224.3. [Q91WT9-2]
XP_006523612.1. XM_006523549.1. [Q91WT9-1]
XP_006523613.1. XM_006523550.1. [Q91WT9-1]
XP_006523614.1. XM_006523551.1. [Q91WT9-1]
UniGeneiMm.206417.
Mm.486781.

Genome annotation databases

EnsembliENSMUST00000067801; ENSMUSP00000066878; ENSMUSG00000024039. [Q91WT9-1]
ENSMUST00000078509; ENSMUSP00000077597; ENSMUSG00000024039. [Q91WT9-2]
ENSMUST00000118504; ENSMUSP00000113209; ENSMUSG00000024039. [Q91WT9-2]
GeneIDi12411.
KEGGimmu:12411.
UCSCiuc008bvl.1. mouse. [Q91WT9-1]
uc008bvm.1. mouse. [Q91WT9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK077669 mRNA. Translation: BAC36943.1 .
BC013472 mRNA. Translation: AAH13472.1 .
BC013480 mRNA. Translation: AAH13480.1 .
BC026595 mRNA. Translation: AAH26595.1 .
CCDSi CCDS28609.1. [Q91WT9-1 ]
CCDS28610.1. [Q91WT9-2 ]
RefSeqi NP_001258282.1. NM_001271353.1. [Q91WT9-2 ]
NP_659104.1. NM_144855.3. [Q91WT9-1 ]
NP_835742.1. NM_178224.3. [Q91WT9-2 ]
XP_006523612.1. XM_006523549.1. [Q91WT9-1 ]
XP_006523613.1. XM_006523550.1. [Q91WT9-1 ]
XP_006523614.1. XM_006523551.1. [Q91WT9-1 ]
UniGenei Mm.206417.
Mm.486781.

3D structure databases

ProteinModelPortali Q91WT9.
SMRi Q91WT9. Positions 40-558.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91WT9. 1 interaction.
MINTi MINT-4121506.
STRINGi 10090.ENSMUSP00000066878.

PTM databases

PhosphoSitei Q91WT9.

Proteomic databases

MaxQBi Q91WT9.
PaxDbi Q91WT9.
PRIDEi Q91WT9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067801 ; ENSMUSP00000066878 ; ENSMUSG00000024039 . [Q91WT9-1 ]
ENSMUST00000078509 ; ENSMUSP00000077597 ; ENSMUSG00000024039 . [Q91WT9-2 ]
ENSMUST00000118504 ; ENSMUSP00000113209 ; ENSMUSG00000024039 . [Q91WT9-2 ]
GeneIDi 12411.
KEGGi mmu:12411.
UCSCi uc008bvl.1. mouse. [Q91WT9-1 ]
uc008bvm.1. mouse. [Q91WT9-2 ]

Organism-specific databases

CTDi 875.
MGIi MGI:88285. Cbs.

Phylogenomic databases

eggNOGi COG0031.
GeneTreei ENSGT00510000047027.
HOGENOMi HOG000217392.
HOVERGENi HBG000918.
InParanoidi Q91WT9.
KOi K01697.
OMAi EEIWTQT.
OrthoDBi EOG7J70F1.
PhylomeDBi Q91WT9.
TreeFami TF300784.

Enzyme and pathway databases

UniPathwayi UPA00136 ; UER00201 .
Reactomei REACT_198986. Cysteine formation from homocysteine.

Miscellaneous databases

ChiTaRSi CBS. mouse.
NextBioi 281194.
PROi Q91WT9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q91WT9.
Bgeei Q91WT9.
CleanExi MM_CBS.
Genevestigatori Q91WT9.

Family and domain databases

InterProi IPR000644. CBS_dom.
IPR005857. Cysta_beta_synth.
IPR001216. P-phosphate_BS.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
[Graphical view ]
Pfami PF00571. CBS. 1 hit.
PF00291. PALP. 1 hit.
[Graphical view ]
SMARTi SM00116. CBS. 1 hit.
[Graphical view ]
SUPFAMi SSF53686. SSF53686. 1 hit.
TIGRFAMsi TIGR01137. cysta_beta. 1 hit.
PROSITEi PS51371. CBS. 1 hit.
PS00901. CYS_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Kidney.

Entry informationi

Entry nameiCBS_MOUSE
AccessioniPrimary (citable) accession number: Q91WT9
Secondary accession number(s): Q91WU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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