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Q91WS4

- BHMT2_MOUSE

UniProt

Q91WS4 - BHMT2_MOUSE

Protein

S-methylmethionine--homocysteine S-methyltransferase BHMT2

Gene

Bhmt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the regulation of homocysteine metabolism. Converts homocysteine to methionine using S-methylmethionine (SMM) as a methyl donor.1 Publication

    Catalytic activityi

    S-methyl-L-methionine + L-homocysteine = 2 L-methionine.1 Publication

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi208 – 2081ZincPROSITE-ProRule annotation
    Metal bindingi290 – 2901ZincPROSITE-ProRule annotation
    Metal bindingi291 – 2911ZincPROSITE-ProRule annotation

    GO - Molecular functioni

    1. betaine-homocysteine S-methyltransferase activity Source: InterPro
    2. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: MGI
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. methionine biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00051; UER00083.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methylmethionine--homocysteine S-methyltransferase BHMT2 (EC:2.1.1.10)
    Short name:
    SMM-hcy methyltransferase
    Alternative name(s):
    Betaine--homocysteine S-methyltransferase 2
    Gene namesi
    Name:Bhmt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1891379. Bhmt2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363S-methylmethionine--homocysteine S-methyltransferase BHMT2PRO_0000273225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei321 – 3211Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ91WS4.
    PRIDEiQ91WS4.

    PTM databases

    PhosphoSiteiQ91WS4.

    Expressioni

    Tissue specificityi

    Expressed in fetal heart, lung, liver, kidney and eye.1 Publication

    Gene expression databases

    BgeeiQ91WS4.
    CleanExiMM_BHMT2.
    GenevestigatoriQ91WS4.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ91WS4.
    SMRiQ91WS4. Positions 10-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 305295Hcy-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Hcy-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0646.
    GeneTreeiENSGT00390000003122.
    HOGENOMiHOG000231636.
    HOVERGENiHBG080367.
    InParanoidiB1B1C9.
    OMAiPEGDMHD.
    OrthoDBiEOG79GT7C.
    PhylomeDBiQ91WS4.
    TreeFamiTF329202.

    Family and domain databases

    Gene3Di3.20.20.330. 1 hit.
    InterProiIPR017226. Betaine-hCys_S-MeTrfase_BHMT.
    IPR003726. S_MeTrfase.
    [Graphical view]
    PfamiPF02574. S-methyl_trans. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037505. Betaine_HMT. 1 hit.
    SUPFAMiSSF82282. SSF82282. 1 hit.
    PROSITEiPS50970. HCY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91WS4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPAGSTRAK KGILERLDSG EVVVGDSGFL FTLEKRGFVK AGLWTPEAVV    50
    EHPSAVRQLH TEFLRAGADV LQTFTFSATE DNMASKWEAV NAAACDLAQE 100
    VAGGGGALVA GGICQTSLYK YHKDETRIKN IFRLQLEVFA RKNVDFLIAE 150
    YFEHVEEAVW AVEVLREVGA PVAVTMCIGP EGDMHDVTPG ECAVKLARAG 200
    ADIIGVNCRF GPWTSLQTMK LMKEGLRDAS LQAHLMVQCL GFHTPDCGKG 250
    GFVDLPEYPF GLEPRVATRW DIQKYAREAY NLGIRYIGGC CGFEPYHIRA 300
    IAEELAPERG FLPPASEKHG SWGSGLNMHT KPWIRARARR EYWENLLPAS 350
    GRPFCPSLSK PDA 363
    Length:363
    Mass (Da):39,872
    Last modified:January 23, 2007 - v2
    Checksum:iC7367F1B10B07EEF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131I → V in AAG41357. (PubMed:11087663)Curated
    Sequence conflicti27 – 271S → G in AAH13515. (PubMed:15489334)Curated
    Sequence conflicti36 – 361R → G in AAG41357. (PubMed:11087663)Curated
    Sequence conflicti327 – 3271N → S in AAG41357. (PubMed:11087663)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF257474 mRNA. Translation: AAG41357.1.
    AK090308 mRNA. Translation: BAC41163.1.
    AK145901 mRNA. Translation: BAE26737.1.
    CT030023, AC158524 Genomic DNA. Translation: CAO77969.1.
    BC013515 mRNA. Translation: AAH13515.1.
    CCDSiCCDS26689.1.
    RefSeqiNP_075022.2. NM_022884.2.
    UniGeneiMm.29981.

    Genome annotation databases

    EnsembliENSMUST00000015941; ENSMUSP00000015941; ENSMUSG00000042118.
    GeneIDi64918.
    KEGGimmu:64918.
    UCSCiuc007rlk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF257474 mRNA. Translation: AAG41357.1 .
    AK090308 mRNA. Translation: BAC41163.1 .
    AK145901 mRNA. Translation: BAE26737.1 .
    CT030023 , AC158524 Genomic DNA. Translation: CAO77969.1 .
    BC013515 mRNA. Translation: AAH13515.1 .
    CCDSi CCDS26689.1.
    RefSeqi NP_075022.2. NM_022884.2.
    UniGenei Mm.29981.

    3D structure databases

    ProteinModelPortali Q91WS4.
    SMRi Q91WS4. Positions 10-363.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q91WS4.

    Proteomic databases

    PaxDbi Q91WS4.
    PRIDEi Q91WS4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015941 ; ENSMUSP00000015941 ; ENSMUSG00000042118 .
    GeneIDi 64918.
    KEGGi mmu:64918.
    UCSCi uc007rlk.1. mouse.

    Organism-specific databases

    CTDi 23743.
    MGIi MGI:1891379. Bhmt2.

    Phylogenomic databases

    eggNOGi COG0646.
    GeneTreei ENSGT00390000003122.
    HOGENOMi HOG000231636.
    HOVERGENi HBG080367.
    InParanoidi B1B1C9.
    OMAi PEGDMHD.
    OrthoDBi EOG79GT7C.
    PhylomeDBi Q91WS4.
    TreeFami TF329202.

    Enzyme and pathway databases

    UniPathwayi UPA00051 ; UER00083 .

    Miscellaneous databases

    NextBioi 320219.
    PROi Q91WS4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91WS4.
    CleanExi MM_BHMT2.
    Genevestigatori Q91WS4.

    Family and domain databases

    Gene3Di 3.20.20.330. 1 hit.
    InterProi IPR017226. Betaine-hCys_S-MeTrfase_BHMT.
    IPR003726. S_MeTrfase.
    [Graphical view ]
    Pfami PF02574. S-methyl_trans. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037505. Betaine_HMT. 1 hit.
    SUPFAMi SSF82282. SSF82282. 1 hit.
    PROSITEi PS50970. HCY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Betaine-homocysteine methyltransferase-2: cDNA cloning, gene sequence, physical mapping, and expression of the human and mouse genes."
      Chadwick L.H., McCandless S.E., Silverman G.L., Schwartz S., Westaway D., Nadeau J.H.
      Genomics 70:66-73(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Gall bladder and Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase."
      Szegedi S.S., Castro C.C., Koutmos M., Garrow T.A.
      J. Biol. Chem. 283:8939-8945(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiBHMT2_MOUSE
    AccessioniPrimary (citable) accession number: Q91WS4
    Secondary accession number(s): B1B1C9, Q8C1U2, Q9EQE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3