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Protein

CDGSH iron-sulfur domain-containing protein 1

Gene

Cisd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions (By similarity).By similarity1 Publication

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Iron-sulfur (2Fe-2S)By similarity
Metal bindingi74 – 741Iron-sulfur (2Fe-2S)By similarity
Metal bindingi83 – 831Iron-sulfur (2Fe-2S)By similarity
Metal bindingi87 – 871Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of cellular respiration Source: MGI
Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CDGSH iron-sulfur domain-containing protein 1
Alternative name(s):
MitoNEET
Gene namesi
Name:Cisd1
Synonyms:D10Ertd214e, Zcd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1261855. Cisd1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3118Helical; Signal-anchor for type III membrane proteinSequence analysisAdd
BLAST
Topological domaini32 – 10877CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 108107CDGSH iron-sulfur domain-containing protein 1PRO_0000089804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki42 – 42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei55 – 551N6-acetyllysine; alternateCombined sources
Cross-linki55 – 55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei68 – 681N6-acetyllysine; alternateCombined sources
Cross-linki68 – 68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki78 – 78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki79 – 79Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki89 – 89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104 – 1041N6-acetyllysine; alternateCombined sources
Cross-linki104 – 104Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki105 – 105Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki106 – 106Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ91WS0.
MaxQBiQ91WS0.
PaxDbiQ91WS0.
PeptideAtlasiQ91WS0.
PRIDEiQ91WS0.

PTM databases

iPTMnetiQ91WS0.
PhosphoSiteiQ91WS0.
SwissPalmiQ91WS0.

Expressioni

Tissue specificityi

Liver, adipose, skeletal muscle and heart (at protein level). Widely expressed. Expressed at the highest levels in the heart.1 Publication

Gene expression databases

BgeeiQ91WS0.
CleanExiMM_CISD1.
GenevisibleiQ91WS0. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ91WS0. 3 interactions.
MINTiMINT-1865788.
STRINGi10090.ENSMUSP00000043559.

Structurei

3D structure databases

ProteinModelPortaliQ91WS0.
SMRiQ91WS0. Positions 43-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CISD protein family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiQ91WS0.
OMAiKDHRNKS.
OrthoDBiEOG7GQXZ6.
PhylomeDBiQ91WS0.
TreeFamiTF324661.

Family and domain databases

InterProiIPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
[Graphical view]
PfamiPF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
[Graphical view]
SMARTiSM00704. ZnF_CDGSH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSSNSAVR VEWIAAVTFA AGTAALGYLA YKKFYAKENR TKAMVNLQIQ
60 70 80 90 100
KDNPKVVHAF DMEDLGDKAV YCRCWRSKKF PFCDGAHIKH NEETGDNVGP

LIIKKKET
Length:108
Mass (Da):12,097
Last modified:December 1, 2001 - v1
Checksum:i16F88E40729C1B49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075907 mRNA. Translation: BAC36046.1.
AK131605 mRNA. Translation: BAE20715.1.
AK131812 mRNA. Translation: BAE20814.1.
AK131995 mRNA. Translation: BAE20925.1.
AK167898 mRNA. Translation: BAE39909.1.
BC013522 mRNA. Translation: AAH13522.1.
BC019860 mRNA. Translation: AAH19860.1.
BC021952 mRNA. Translation: AAH21952.1.
CCDSiCCDS23917.1.
RefSeqiNP_598768.1. NM_134007.4.
UniGeneiMm.254114.

Genome annotation databases

EnsembliENSMUST00000045887; ENSMUSP00000043559; ENSMUSG00000037710.
GeneIDi52637.
KEGGimmu:52637.
UCSCiuc007foq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075907 mRNA. Translation: BAC36046.1.
AK131605 mRNA. Translation: BAE20715.1.
AK131812 mRNA. Translation: BAE20814.1.
AK131995 mRNA. Translation: BAE20925.1.
AK167898 mRNA. Translation: BAE39909.1.
BC013522 mRNA. Translation: AAH13522.1.
BC019860 mRNA. Translation: AAH19860.1.
BC021952 mRNA. Translation: AAH21952.1.
CCDSiCCDS23917.1.
RefSeqiNP_598768.1. NM_134007.4.
UniGeneiMm.254114.

3D structure databases

ProteinModelPortaliQ91WS0.
SMRiQ91WS0. Positions 43-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91WS0. 3 interactions.
MINTiMINT-1865788.
STRINGi10090.ENSMUSP00000043559.

PTM databases

iPTMnetiQ91WS0.
PhosphoSiteiQ91WS0.
SwissPalmiQ91WS0.

Proteomic databases

EPDiQ91WS0.
MaxQBiQ91WS0.
PaxDbiQ91WS0.
PeptideAtlasiQ91WS0.
PRIDEiQ91WS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045887; ENSMUSP00000043559; ENSMUSG00000037710.
GeneIDi52637.
KEGGimmu:52637.
UCSCiuc007foq.1. mouse.

Organism-specific databases

CTDi55847.
MGIiMGI:1261855. Cisd1.

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiQ91WS0.
OMAiKDHRNKS.
OrthoDBiEOG7GQXZ6.
PhylomeDBiQ91WS0.
TreeFamiTF324661.

Miscellaneous databases

PROiQ91WS0.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WS0.
CleanExiMM_CISD1.
GenevisibleiQ91WS0. MM.

Family and domain databases

InterProiIPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
[Graphical view]
PfamiPF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
[Graphical view]
SMARTiSM00704. ZnF_CDGSH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BALB/cJ and C57BL/6J.
    Tissue: Pancreas and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Liver.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 43-51; 56-68 AND 90-104, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "MitoNEET is an iron-containing outer mitochondrial membrane protein that regulates oxidative capacity."
    Wiley S.E., Murphy A.N., Ross S.A., van der Geer P., Dixon J.E.
    Proc. Natl. Acad. Sci. U.S.A. 104:5318-5323(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-55; LYS-68 AND LYS-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCISD1_MOUSE
AccessioniPrimary (citable) accession number: Q91WS0
Secondary accession number(s): Q3V2I3, Q8WUQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.