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Q91WQ3

- SYYC_MOUSE

UniProt

Q91WQ3 - SYYC_MOUSE

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Protein

Tyrosine--tRNA ligase, cytoplasmic

Gene

Yars

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391TyrosineBy similarity
Binding sitei166 – 1661TyrosineBy similarity
Binding sitei170 – 1701TyrosineBy similarity
Binding sitei173 – 1731TyrosineBy similarity
Binding sitei188 – 1881TyrosineBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: Ensembl
  3. tRNA binding Source: UniProtKB-KW
  4. tyrosine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. tyrosyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
Cleaved into the following chain:
Gene namesi
Name:Yars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2147627. Yars.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Tyrosine--tRNA ligase, cytoplasmicPRO_0000055674Add
BLAST
Initiator methioninei1 – 11Removed; alternateBy similarity
Chaini2 – 528527Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedPRO_0000423286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylglycine; in Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedBy similarity
Modified residuei197 – 1971N6-acetyllysineBy similarity
Modified residuei206 – 2061N6-acetyllysineBy similarity
Modified residuei474 – 4741N6-acetyllysineBy similarity
Modified residuei482 – 4821N6-acetyllysineBy similarity
Modified residuei490 – 4901N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ91WQ3.
PaxDbiQ91WQ3.
PRIDEiQ91WQ3.

PTM databases

PhosphoSiteiQ91WQ3.

Expressioni

Gene expression databases

BgeeiQ91WQ3.
CleanExiMM_YARS.
ExpressionAtlasiQ91WQ3. baseline and differential.
GenevestigatoriQ91WQ3.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi223230. 1 interaction.
IntActiQ91WQ3. 2 interactions.
MINTiMINT-1855454.

Structurei

3D structure databases

ProteinModelPortaliQ91WQ3.
SMRiQ91WQ3. Positions 4-342, 360-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini364 – 468105tRNA-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 529"HIGH" region
Motifi222 – 2265"KMSKS" region

Sequence similaritiesi

Contains 1 tRNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0162.
HOGENOMiHOG000228237.
HOVERGENiHBG080113.
InParanoidiQ91WQ3.
KOiK01866.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-ligase.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WQ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKVYW GTATTGKPHV
60 70 80 90 100
AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTSYYENV
110 120 130 140 150
IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDAKKAGA
160 170 180 190 200
EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGVDQRK IFTFAEKYLP
210 220 230 240 250
ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC
260 270 280 290 300
EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT VYLELEKDFA
310 320 330 340 350
AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPP
360 370 380 390 400
AKGPAKNSEP EEVIPSRLDI RVGKILSVEK HPDADSLYVE KIDVGEAEPR
410 420 430 440 450
TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVDSQGML LCASVEGVSR
460 470 480 490 500
QVEPLDPPAG SAPGERVFVQ GYEKGQPDEE LKPKKKVFEK LQADFKISEE
510 520
CIAQWKQTNF MTKLGFVSCK SLKGGNIS
Length:528
Mass (Da):59,105
Last modified:January 23, 2007 - v3
Checksum:i39D852D4C5701D0B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111L → M in BAC26120. (PubMed:16141072)Curated
Sequence conflicti29 – 291E → K in BAE24073. (PubMed:16141072)Curated
Sequence conflicti195 – 1951A → S in BAC36424. (PubMed:16141072)Curated
Sequence conflicti294 – 2941E → Q in BAC36424. (PubMed:16141072)Curated
Sequence conflicti320 – 3201L → W in BAC36424. (PubMed:16141072)Curated
Sequence conflicti377 – 3771S → R in BAC36424. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028785 mRNA. Translation: BAC26120.1.
AK043837 mRNA. Translation: BAC31674.1.
AK076634 mRNA. Translation: BAC36424.1.
AK139579 mRNA. Translation: BAE24073.1.
AK145356 mRNA. Translation: BAE26384.1.
AK151880 mRNA. Translation: BAE30766.1.
AK169708 mRNA. Translation: BAE41320.1.
BC013552 mRNA. Translation: AAH13552.1.
BC022143 mRNA. Translation: AAH22143.1.
BC026615 mRNA. Translation: AAH26615.1.
RefSeqiNP_598912.4. NM_134151.4.
UniGeneiMm.145488.

Genome annotation databases

GeneIDi107271.
KEGGimmu:107271.
UCSCiuc008uwk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028785 mRNA. Translation: BAC26120.1 .
AK043837 mRNA. Translation: BAC31674.1 .
AK076634 mRNA. Translation: BAC36424.1 .
AK139579 mRNA. Translation: BAE24073.1 .
AK145356 mRNA. Translation: BAE26384.1 .
AK151880 mRNA. Translation: BAE30766.1 .
AK169708 mRNA. Translation: BAE41320.1 .
BC013552 mRNA. Translation: AAH13552.1 .
BC022143 mRNA. Translation: AAH22143.1 .
BC026615 mRNA. Translation: AAH26615.1 .
RefSeqi NP_598912.4. NM_134151.4.
UniGenei Mm.145488.

3D structure databases

ProteinModelPortali Q91WQ3.
SMRi Q91WQ3. Positions 4-342, 360-528.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 223230. 1 interaction.
IntActi Q91WQ3. 2 interactions.
MINTi MINT-1855454.

PTM databases

PhosphoSitei Q91WQ3.

Proteomic databases

MaxQBi Q91WQ3.
PaxDbi Q91WQ3.
PRIDEi Q91WQ3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 107271.
KEGGi mmu:107271.
UCSCi uc008uwk.2. mouse.

Organism-specific databases

CTDi 8565.
MGIi MGI:2147627. Yars.

Phylogenomic databases

eggNOGi COG0162.
HOGENOMi HOG000228237.
HOVERGENi HBG080113.
InParanoidi Q91WQ3.
KOi K01866.

Miscellaneous databases

ChiTaRSi Yars. mouse.
NextBioi 358652.
PROi Q91WQ3.
SOURCEi Search...

Gene expression databases

Bgeei Q91WQ3.
CleanExi MM_YARS.
ExpressionAtlasi Q91WQ3. baseline and differential.
Genevestigatori Q91WQ3.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.620. 1 hit.
InterProi IPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-ligase.
[Graphical view ]
Pfami PF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view ]
PRINTSi PR01040. TRNASYNTHTYR.
SUPFAMi SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00234. tyrS. 1 hit.
PROSITEi PS50886. TRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Brain cortex, Egg, Embryo, Skin, Testis and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Liver.

Entry informationi

Entry nameiSYYC_MOUSE
AccessioniPrimary (citable) accession number: Q91WQ3
Secondary accession number(s): Q3TEC8
, Q3U9A1, Q3UTA7, Q8BVT2, Q8C183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3