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Protein

Serine protease inhibitor A3N

Gene

Serpina3n

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei381 – 3822Reactive bondBy similarity

GO - Molecular functioni

GO - Biological processi

  • acute-phase response Source: UniProtKB-KW
  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Acute phase

Protein family/group databases

MEROPSiI04.078.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor A3N
Short name:
Serpin A3N
Gene namesi
Name:Serpina3n
Synonyms:Spi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105045. Serpina3n.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 418398Serine protease inhibitor A3NPRO_0000032419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence analysis
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ91WP6.
MaxQBiQ91WP6.
PaxDbiQ91WP6.
PRIDEiQ91WP6.

PTM databases

iPTMnetiQ91WP6.
PhosphoSiteiQ91WP6.

Expressioni

Tissue specificityi

Expressed at high levels in brain, heart, liver, lung, spleen, testis and thymus, and at low levels in bone marrow, kidney and skeletal muscle.2 Publications

Inductioni

Expression is induced by bacterial lipopolysaccharide (LPS).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021506.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi48 – 6518Combined sources
Beta strandi71 – 733Combined sources
Helixi75 – 8612Combined sources
Helixi91 – 10010Combined sources
Turni105 – 1073Combined sources
Helixi110 – 12415Combined sources
Beta strandi131 – 14212Combined sources
Helixi149 – 15911Combined sources
Beta strandi162 – 1665Combined sources
Helixi171 – 18515Combined sources
Turni186 – 1883Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi203 – 21210Combined sources
Beta strandi214 – 2174Combined sources
Helixi221 – 2233Combined sources
Beta strandi225 – 2295Combined sources
Beta strandi237 – 25418Combined sources
Turni255 – 2584Combined sources
Beta strandi259 – 27719Combined sources
Helixi282 – 2876Combined sources
Helixi291 – 30010Combined sources
Beta strandi302 – 31211Combined sources
Beta strandi314 – 3218Combined sources
Helixi323 – 3286Combined sources
Helixi333 – 3353Combined sources
Helixi342 – 3454Combined sources
Beta strandi346 – 3483Combined sources
Beta strandi354 – 36310Combined sources
Beta strandi365 – 3695Combined sources
Beta strandi389 – 3913Combined sources
Beta strandi396 – 4027Combined sources
Turni403 – 4053Combined sources
Beta strandi409 – 4157Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YXAX-ray2.10A/B21-418[»]
ProteinModelPortaliQ91WP6.
SMRiQ91WP6. Positions 47-418.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91WP6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni367 – 39226RCLAdd
BLAST

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiQ91WP6.
PhylomeDBiQ91WP6.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFIAALGLL MAGICPAVLC FPDGTLGMDA AVQEDHDNGT QLDSLTLASI
60 70 80 90 100
NTDFAFSLYK ELVLKNPDKN IVFSPLSISA ALAVMSLGAK GNTLEEILEG
110 120 130 140 150
LKFNLTETSE ADIHQGFGHL LQRLNQPKDQ VQISTGSALF IEKRQQILTE
160 170 180 190 200
FQEKAKTLYQ AEAFTADFQQ PRQAKKLIND YVRKQTQGMI KELVSDLDKR
210 220 230 240 250
TLMVLVNYIY FKAKWKVPFD PLDTFKSEFY AGKRRPVIVP MMSMEDLTTP
260 270 280 290 300
YFRDEELSCT VVELKYTGNA SALFILPDQG RMQQVEASLQ PETLRKWKNS
310 320 330 340 350
LKPRMIDELH LPKFSISTDY SLEDVLSKLG IREVFSTQAD LSAITGTKDL
360 370 380 390 400
RVSQVVHKAV LDVAETGTEA AAATGVKFVP MSAKLYPLTV YFNRPFLIMI
410
FDTETEIAPF IAKIANPK
Length:418
Mass (Da):46,718
Last modified:December 1, 2001 - v1
Checksum:i4BF21BBE10A81F6C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311A → C in AAA40130 (PubMed:1718822).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64086 mRNA. Translation: AAA40130.1.
BC013651 mRNA. Translation: AAH13651.1.
CCDSiCCDS26152.1.
PIRiJH0494.
UniGeneiMm.482074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64086 mRNA. Translation: AAA40130.1.
BC013651 mRNA. Translation: AAH13651.1.
CCDSiCCDS26152.1.
PIRiJH0494.
UniGeneiMm.482074.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YXAX-ray2.10A/B21-418[»]
ProteinModelPortaliQ91WP6.
SMRiQ91WP6. Positions 47-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021506.

Protein family/group databases

MEROPSiI04.078.

PTM databases

iPTMnetiQ91WP6.
PhosphoSiteiQ91WP6.

Proteomic databases

EPDiQ91WP6.
MaxQBiQ91WP6.
PaxDbiQ91WP6.
PRIDEiQ91WP6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:105045. Serpina3n.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiQ91WP6.
PhylomeDBiQ91WP6.

Miscellaneous databases

EvolutionaryTraceiQ91WP6.
PROiQ91WP6.
SOURCEiSearch...

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two cDNAs encoding novel alpha-1-antichymotrypsin-like proteins in a murine chondrocytic cell line."
    Inglis J.D., Lee M., Davidson D.R., Hill R.E.
    Gene 106:213-220(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: 129.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "A review and comparison of the murine alpha1-antitrypsin and alpha1-antichymotrypsin multigene clusters with the human clade A serpins."
    Forsyth S., Horvath A., Coughlin P.
    Genomics 81:336-345(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  4. "Expression patterns of murine antichymotrypsin-like genes reflect evolutionary divergence at the Serpina3 locus."
    Horvath A.J., Forsyth S.L., Coughlin P.B.
    J. Mol. Evol. 59:488-497(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, REGION RCL.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.
  6. "The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins."
    Horvath A.J., Irving J.A., Rossjohn J., Law R.H., Bottomley S.P., Quinsey N.S., Pike R.N., Coughlin P.B., Whisstock J.C.
    J. Biol. Chem. 280:43168-43178(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 21-418.

Entry informationi

Entry nameiSPA3N_MOUSE
AccessioniPrimary (citable) accession number: Q91WP6
Secondary accession number(s): Q62258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 14 individual murine paralogs.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.