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Protein

Mannan-binding lectin serine protease 2

Gene

Masp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase (By similarity).By similarity

Catalytic activityi

Selective cleavage after Arg-223 in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Calcium 1By similarity1
Metal bindingi75Calcium 1By similarity1
Metal bindingi120Calcium 1By similarity1
Metal bindingi122Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi123Calcium 1By similarity1
Metal bindingi138Calcium 2By similarity1
Metal bindingi141Calcium 2By similarity1
Metal bindingi158Calcium 2By similarity1
Metal bindingi162Calcium 2; via carbonyl oxygenBy similarity1
Active sitei483Charge relay systemBy similarity1
Active sitei532Charge relay systemBy similarity1
Active sitei632Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.104. 3474.
ReactomeiR-MMU-166662. Lectin pathway of complement activation.
R-MMU-166663. Initial triggering of complement.
R-MMU-2855086. Ficolins bind to repetitive carbohydrate structures on the target cell surface.

Protein family/group databases

MEROPSiS01.229.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan-binding lectin serine protease 2 (EC:3.4.21.104)
Alternative name(s):
MBL-associated serine protease 2
Mannose-binding protein-associated serine protease 2
Short name:
MASP-2
Cleaved into the following 2 chains:
Gene namesi
Name:Masp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1330832. Masp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000002760120 – 685Mannan-binding lectin serine protease 2Add BLAST666
ChainiPRO_000002760220 – 443Mannan-binding lectin serine protease 2 A chainAdd BLAST424
ChainiPRO_0000027603444 – 685Mannan-binding lectin serine protease 2 B chainAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi72 ↔ 90By similarity
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi142 ↔ 156By similarity
Disulfide bondi152 ↔ 165By similarity
Modified residuei158(3R)-3-hydroxyasparagineSequence analysis1
Disulfide bondi167 ↔ 180By similarity
Disulfide bondi184 ↔ 211By similarity
Disulfide bondi241 ↔ 259By similarity
Glycosylationi285N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi300 ↔ 348By similarity
Glycosylationi308N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi328 ↔ 361By similarity
Disulfide bondi366 ↔ 411By similarity
Disulfide bondi396 ↔ 429By similarity
Disulfide bondi433 ↔ 552Interchain (between A and B chains)PROSITE-ProRule annotation
Glycosylationi545N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi598 ↔ 617By similarity
Disulfide bondi628 ↔ 659By similarity
Glycosylationi641N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei443 – 444CleavageBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiQ91WP0.
PeptideAtlasiQ91WP0.
PRIDEiQ91WP0.

PTM databases

iPTMnetiQ91WP0.
PhosphoSitePlusiQ91WP0.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiENSMUSG00000028979.
CleanExiMM_MASP2.
GenevisibleiQ91WP0. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Binds MBL2. Isoform 2 binds to MASP1. Binds SERPING1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049729.

Structurei

3D structure databases

ProteinModelPortaliQ91WP0.
SMRiQ91WP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 137CUB 1PROSITE-ProRule annotationAdd BLAST118
Domaini138 – 181EGF-like; calcium-bindingAdd BLAST44
Domaini184 – 296CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini298 – 363Sushi 1PROSITE-ProRule annotationAdd BLAST66
Domaini364 – 431Sushi 2PROSITE-ProRule annotationAdd BLAST68
Domaini444 – 683Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOVERGENiHBG000559.
InParanoidiQ91WP0.
KOiK03993.
OMAiWTLTAPP.
OrthoDBiEOG091G02DS.
PhylomeDBiQ91WP0.
TreeFamiTF330373.

Family and domain databases

CDDicd00033. CCP. 2 hits.
cd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLLIFLGLL WSLVATLLGS KWPEPVFGRL VSPGFPEKYA DHQDRSWTLT
60 70 80 90 100
APPGYRLRLY FTHFDLELSY RCEYDFVKLS SGTKVLATLC GQESTDTEQA
110 120 130 140 150
PGNDTFYSLG PSLKVTFHSD YSNEKPFTGF EAFYAAEDVD ECRVSLGDSV
160 170 180 190 200
PCDHYCHNYL GGYYCSCRAG YVLHQNKHTC SALCSGQVFT GRSGYLSSPE
210 220 230 240 250
YPQPYPKLSS CTYSIRLEDG FSVILDFVES FDVETHPEAQ CPYDSLKIQT
260 270 280 290 300
DKGEHGPFCG KTLPPRIETD SHKVTITFAT DESGNHTGWK IHYTSTARPC
310 320 330 340 350
PDPTAPPNGS ISPVQAIYVL KDRFSVFCKT GFELLQGSVP LKSFTAVCQK
360 370 380 390 400
DGSWDRPMPE CSIIDCGPPD DLPNGHVDYI TGPEVTTYKA VIQYSCEETF
410 420 430 440 450
YTMSSNGKYV CEADGFWTSS KGEKLPPVCE PVCGLSTHTI GGRIVGGQPA
460 470 480 490 500
KPGDFPWQVL LLGQTTAAAG ALIHDNWVLT AAHAVYEKRM AASSLNIRMG
510 520 530 540 550
ILKRLSPHYT QAWPEEIFIH EGYTHGAGFD NDIALIKLKN KVTINGSIMP
560 570 580 590 600
VCLPRKEAAS LMRTDFTGTV AGWGLTQKGL LARNLMFVDI PIADHQKCTA
610 620 630 640 650
VYEKLYPGVR VSANMLCAGL ETGGKDSCRG DSGGALVFLD NETQRWFVGG
660 670 680
IVSWGSINCG AADQYGVYTK VINYIPWIEN IISNF
Length:685
Mass (Da):75,517
Last modified:December 1, 2001 - v1
Checksum:iF56A6D522BC7099D
GO
Isoform 2 (identifier: Q91WP0-2) [UniParc]FASTAAdd to basket
Also known as: MAp19

The sequence of this isoform differs from the canonical sequence as follows:
     182-185: ALCS → EQSL
     186-685: Missing.

Show »
Length:185
Mass (Da):20,974
Checksum:i72E01900D30A18B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 2MR → MSLPCPQ (PubMed:10586086).Curated2
Sequence conflicti172V → I in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti172V → I in CAB65247 (PubMed:10586086).Curated1
Sequence conflicti192R → K in CAB65250 (PubMed:10586086).Curated1
Sequence conflicti317I → T in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti325S → Y in CAB65250 (PubMed:10586086).Curated1
Sequence conflicti384E → Q in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti600A → T in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti663D → G in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti678I → N in BAA34674 (PubMed:9794427).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014636182 – 185ALCS → EQSL in isoform 2. 1 Publication4
Alternative sequenceiVSP_014637186 – 685Missing in isoform 2. 1 PublicationAdd BLAST500

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009459 mRNA. Translation: BAA34674.1.
AJ250369 mRNA. Translation: CAB63701.1.
Y19160 mRNA. Translation: CAB65247.1.
Y19163 mRNA. Translation: CAB65250.1.
AK050052 mRNA. Translation: BAC34052.1.
AL606969 Genomic DNA. Translation: CAM21185.1.
AL606969 Genomic DNA. Translation: CAM21186.1.
BC013893 mRNA. Translation: AAH13893.1.
CCDSiCCDS18941.1. [Q91WP0-1]
CCDS18942.1. [Q91WP0-2]
RefSeqiNP_001003893.1. NM_001003893.2. [Q91WP0-1]
NP_034897.1. NM_010767.3. [Q91WP0-2]
UniGeneiMm.378962.

Genome annotation databases

EnsembliENSMUST00000052060; ENSMUSP00000049729; ENSMUSG00000028979. [Q91WP0-1]
ENSMUST00000105701; ENSMUSP00000101326; ENSMUSG00000028979. [Q91WP0-2]
GeneIDi17175.
KEGGimmu:17175.
UCSCiuc008vux.1. mouse. [Q91WP0-2]
uc008vuy.1. mouse. [Q91WP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009459 mRNA. Translation: BAA34674.1.
AJ250369 mRNA. Translation: CAB63701.1.
Y19160 mRNA. Translation: CAB65247.1.
Y19163 mRNA. Translation: CAB65250.1.
AK050052 mRNA. Translation: BAC34052.1.
AL606969 Genomic DNA. Translation: CAM21185.1.
AL606969 Genomic DNA. Translation: CAM21186.1.
BC013893 mRNA. Translation: AAH13893.1.
CCDSiCCDS18941.1. [Q91WP0-1]
CCDS18942.1. [Q91WP0-2]
RefSeqiNP_001003893.1. NM_001003893.2. [Q91WP0-1]
NP_034897.1. NM_010767.3. [Q91WP0-2]
UniGeneiMm.378962.

3D structure databases

ProteinModelPortaliQ91WP0.
SMRiQ91WP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049729.

Protein family/group databases

MEROPSiS01.229.

PTM databases

iPTMnetiQ91WP0.
PhosphoSitePlusiQ91WP0.

Proteomic databases

PaxDbiQ91WP0.
PeptideAtlasiQ91WP0.
PRIDEiQ91WP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052060; ENSMUSP00000049729; ENSMUSG00000028979. [Q91WP0-1]
ENSMUST00000105701; ENSMUSP00000101326; ENSMUSG00000028979. [Q91WP0-2]
GeneIDi17175.
KEGGimmu:17175.
UCSCiuc008vux.1. mouse. [Q91WP0-2]
uc008vuy.1. mouse. [Q91WP0-1]

Organism-specific databases

CTDi10747.
MGIiMGI:1330832. Masp2.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOVERGENiHBG000559.
InParanoidiQ91WP0.
KOiK03993.
OMAiWTLTAPP.
OrthoDBiEOG091G02DS.
PhylomeDBiQ91WP0.
TreeFamiTF330373.

Enzyme and pathway databases

BRENDAi3.4.21.104. 3474.
ReactomeiR-MMU-166662. Lectin pathway of complement activation.
R-MMU-166663. Initial triggering of complement.
R-MMU-2855086. Ficolins bind to repetitive carbohydrate structures on the target cell surface.

Miscellaneous databases

ChiTaRSiMasp2. mouse.
PROiQ91WP0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028979.
CleanExiMM_MASP2.
GenevisibleiQ91WP0. MM.

Family and domain databases

CDDicd00033. CCP. 2 hits.
cd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMASP2_MOUSE
AccessioniPrimary (citable) accession number: Q91WP0
Secondary accession number(s): B1ARY2
, B1ARY3, Q9QXA4, Q9QXD2, Q9QXD5, Q9Z338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Dimerization and MBL2 binding requires calcium ions.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.