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Protein

Kynurenine 3-monooxygenase

Gene

Kmo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.UniRule annotation1 Publication

Catalytic activityi

L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.UniRule annotationBy similarity1 Publication

Cofactori

FADUniRule annotationBy similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (Kmo)
  2. Kynureninase (Kynu), Kynureninase (Kynu), Kynureninase (Kynu)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (Haao)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19FAD; via amide nitrogenBy similarity1
Binding sitei57FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei85L-kynurenineBy similarity1
Binding sitei99L-kynurenineBy similarity1
Binding sitei111FADBy similarity1
Binding sitei136FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei172FADBy similarity1
Binding sitei304FAD; via amide nitrogenBy similarity1
Binding sitei363L-kynurenineBy similarity1
Binding sitei398L-kynurenineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 40FADBy similarity4
Nucleotide bindingi317 – 318FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-MMU-71240 Tryptophan catabolism
UniPathwayiUPA00253; UER00328

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine 3-monooxygenaseUniRule annotation (EC:1.14.13.9UniRule annotation)
Alternative name(s):
Kynurenine 3-hydroxylaseUniRule annotation
Gene namesi
Name:KmoImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2138151 Kmo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei385 – 404HelicalUniRule annotationAdd BLAST20
Transmembranei425 – 445HelicalUniRule annotationAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show normal fecundity, fertility and longevity up tp 2 year. They have reduced serum 3-hydroxy-L-kynurenine concentrations. They are protected against extrapancreatic tissue injury to the lung, kidney and liver produced by acute pancreatitis-induced multiple organ dysfunction syndrome.1 Publication

Chemistry databases

ChEMBLiCHEMBL3407318

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002297431 – 479Kynurenine 3-monooxygenaseCuratedAdd BLAST479

Proteomic databases

PaxDbiQ91WN4
PeptideAtlasiQ91WN4
PRIDEiQ91WN4

PTM databases

iPTMnetiQ91WN4
PhosphoSitePlusiQ91WN4
SwissPalmiQ91WN4

Expressioni

Tissue specificityi

Expressed by organs containing secondary lymphoid tissue, such as the lung, spleen, mesenteric lymph node, thymus and peripheral lymph nodes.1 Publication

Gene expression databases

BgeeiENSMUSG00000039783
ExpressionAtlasiQ91WN4 baseline and differential
GenevisibleiQ91WN4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038914

Chemistry databases

BindingDBiQ91WN4

Structurei

3D structure databases

ProteinModelPortaliQ91WN4
SMRiQ91WN4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Transmembrane domains are required for enzymatic activity.By similarity

Sequence similaritiesi

Belongs to the aromatic-ring hydroxylase family. KMO subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2614 Eukaryota
COG0654 LUCA
GeneTreeiENSGT00390000000747
HOGENOMiHOG000251788
HOVERGENiHBG057213
InParanoidiQ91WN4
KOiK00486
OMAiMPSTFIP
OrthoDBiEOG091G0769
PhylomeDBiQ91WN4
TreeFamiTF312990

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_01971 Kynurenine_monooxygenase, 1 hit
InterProiView protein in InterPro
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
IPR027545 Kynurenine_monooxygenase
PfamiView protein in Pfam
PF01494 FAD_binding_3, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q91WN4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSDTQGKR VAVIGGGLVG ALNACFLAKR NFQVDVYEAR EDIRVAKSAR
60 70 80 90 100
GRSINLALSY RGRQALKAIG LEDQIVSKGV PMKARMIHSL SGKKSAIPYG
110 120 130 140 150
NKSQYILSIS RENLNKDLLT AVESYANAKV HFGHKLSKCI PEEGVLTVLG
160 170 180 190 200
PDKVPRDVTC DLVVGCDGAY STVRAHLMKK PRFDYTQQYI PHGYMELTIP
210 220 230 240 250
PKNGEYAMEP NCLHIWPRNA YMMIALPNMD KSFTCTLFMP FEEFERLPTR
260 270 280 290 300
SDVLDFFQKN FPDAIPLMGE QALMRDFFLL PAQPMISVKC SPFHLKSHCV
310 320 330 340 350
LMGDAAHAIV PFFGQGMNAG FEDCLVFDEL MDKFNNNLSM CLPEFSRFRI
360 370 380 390 400
PDDHAISDLS MYNYIEMRAH VNSRWFLFQK LLDKFLHAIM PSTFIPLYTM
410 420 430 440 450
VAFTRIRYHE AVLRWHWQKK VINRGLFVLG SLIAIGGTYL LVHHLSLRPL
460 470
EFLRRPAWMG TTGYWTRSTD ISLQVPWSY
Length:479
Mass (Da):54,532
Last modified:December 1, 2001 - v1
Checksum:iE880997DBC9C8163
GO
Isoform 21 Publication (identifier: Q91WN4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-400: Missing.

Note: No experimental confirmation available.
Show »
Length:445
Mass (Da):50,396
Checksum:iE2B58D64DDF95BCC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051974367 – 400Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129011 mRNA No translation available.
BC014683 mRNA Translation: AAH14683.1
CCDSiCCDS15548.1 [Q91WN4-1]
RefSeqiNP_598570.1, NM_133809.1 [Q91WN4-1]
UniGeneiMm.27217

Genome annotation databases

EnsembliENSMUST00000040250; ENSMUSP00000038914; ENSMUSG00000039783 [Q91WN4-1]
ENSMUST00000097458; ENSMUSP00000095067; ENSMUSG00000039783 [Q91WN4-2]
GeneIDi98256
KEGGimmu:98256
UCSCiuc007dto.1 mouse [Q91WN4-1]
uc011wxd.1 mouse [Q91WN4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKMO_MOUSE
AccessioniPrimary (citable) accession number: Q91WN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health