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Protein

Kynurenine 3-monooxygenase

Gene

Kmo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.UniRule annotation

Catalytic activityi

L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.By similarityUniRule annotation

Cofactori

FADBy similarityUniRule annotation

Pathwayi

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: MGI
  2. kynurenine 3-monooxygenase activity Source: UniProtKB
  3. NAD(P)H oxidase activity Source: MGI

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. kynurenine metabolic process Source: MGI
  4. NAD metabolic process Source: UniProtKB
  5. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  6. response to salt stress Source: MGI
  7. secondary metabolite biosynthetic process Source: GO_Central
  8. tryptophan catabolic process to kynurenine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiREACT_240936. Tryptophan catabolism.
UniPathwayiUPA00253; UER00328.

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine 3-monooxygenaseUniRule annotation (EC:1.14.13.9UniRule annotation)
Alternative name(s):
Kynurenine 3-hydroxylaseUniRule annotation
Gene namesi
Name:KmoImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2138151. Kmo.

Subcellular locationi

Mitochondrion outer membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei385 – 40420HelicalUniRule annotationAdd
BLAST
Transmembranei425 – 44521HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial inner membrane Source: MGI
  4. mitochondrial outer membrane Source: UniProtKB
  5. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Kynurenine 3-monooxygenaseCuratedPRO_0000229743Add
BLAST

Proteomic databases

MaxQBiQ91WN4.
PaxDbiQ91WN4.
PRIDEiQ91WN4.

PTM databases

PhosphoSiteiQ91WN4.

Expressioni

Gene expression databases

BgeeiQ91WN4.
ExpressionAtlasiQ91WN4. baseline and differential.
GenevestigatoriQ91WN4.

Structurei

3D structure databases

ProteinModelPortaliQ91WN4.
SMRiQ91WN4. Positions 11-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aromatic-ring hydroxylase family. KMO subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0654.
GeneTreeiENSGT00390000000747.
HOGENOMiHOG000251788.
HOVERGENiHBG057213.
InParanoidiQ91WN4.
KOiK00486.
OMAiQPMISVK.
PhylomeDBiQ91WN4.
TreeFamiTF312990.

Family and domain databases

HAMAPiMF_01971. Kynurenine_monooxygenase.
InterProiIPR027545. Kynurenine_monooxygenase.
IPR002938. mOase_FAD-bd.
IPR003042. Rng_hydrolase-like.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
PRINTSiPR00420. RNGMNOXGNASE.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q91WN4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSDTQGKR VAVIGGGLVG ALNACFLAKR NFQVDVYEAR EDIRVAKSAR
60 70 80 90 100
GRSINLALSY RGRQALKAIG LEDQIVSKGV PMKARMIHSL SGKKSAIPYG
110 120 130 140 150
NKSQYILSIS RENLNKDLLT AVESYANAKV HFGHKLSKCI PEEGVLTVLG
160 170 180 190 200
PDKVPRDVTC DLVVGCDGAY STVRAHLMKK PRFDYTQQYI PHGYMELTIP
210 220 230 240 250
PKNGEYAMEP NCLHIWPRNA YMMIALPNMD KSFTCTLFMP FEEFERLPTR
260 270 280 290 300
SDVLDFFQKN FPDAIPLMGE QALMRDFFLL PAQPMISVKC SPFHLKSHCV
310 320 330 340 350
LMGDAAHAIV PFFGQGMNAG FEDCLVFDEL MDKFNNNLSM CLPEFSRFRI
360 370 380 390 400
PDDHAISDLS MYNYIEMRAH VNSRWFLFQK LLDKFLHAIM PSTFIPLYTM
410 420 430 440 450
VAFTRIRYHE AVLRWHWQKK VINRGLFVLG SLIAIGGTYL LVHHLSLRPL
460 470
EFLRRPAWMG TTGYWTRSTD ISLQVPWSY
Length:479
Mass (Da):54,532
Last modified:December 1, 2001 - v1
Checksum:iE880997DBC9C8163
GO
Isoform 21 Publication (identifier: Q91WN4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-400: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:445
Mass (Da):50,396
Checksum:iE2B58D64DDF95BCC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei367 – 40034Missing in isoform 2. 1 PublicationVSP_051974Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129011 mRNA. No translation available.
BC014683 mRNA. Translation: AAH14683.1.
CCDSiCCDS15548.1. [Q91WN4-1]
RefSeqiNP_598570.1. NM_133809.1. [Q91WN4-1]
UniGeneiMm.27217.

Genome annotation databases

EnsembliENSMUST00000040250; ENSMUSP00000038914; ENSMUSG00000039783. [Q91WN4-1]
ENSMUST00000097458; ENSMUSP00000095067; ENSMUSG00000039783. [Q91WN4-2]
GeneIDi98256.
KEGGimmu:98256.
UCSCiuc007dto.1. mouse. [Q91WN4-1]
uc011wxd.1. mouse. [Q91WN4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129011 mRNA. No translation available.
BC014683 mRNA. Translation: AAH14683.1.
CCDSiCCDS15548.1. [Q91WN4-1]
RefSeqiNP_598570.1. NM_133809.1. [Q91WN4-1]
UniGeneiMm.27217.

3D structure databases

ProteinModelPortaliQ91WN4.
SMRiQ91WN4. Positions 11-367.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ91WN4.

Proteomic databases

MaxQBiQ91WN4.
PaxDbiQ91WN4.
PRIDEiQ91WN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040250; ENSMUSP00000038914; ENSMUSG00000039783. [Q91WN4-1]
ENSMUST00000097458; ENSMUSP00000095067; ENSMUSG00000039783. [Q91WN4-2]
GeneIDi98256.
KEGGimmu:98256.
UCSCiuc007dto.1. mouse. [Q91WN4-1]
uc011wxd.1. mouse. [Q91WN4-2]

Organism-specific databases

CTDi8564.
MGIiMGI:2138151. Kmo.

Phylogenomic databases

eggNOGiCOG0654.
GeneTreeiENSGT00390000000747.
HOGENOMiHOG000251788.
HOVERGENiHBG057213.
InParanoidiQ91WN4.
KOiK00486.
OMAiQPMISVK.
PhylomeDBiQ91WN4.
TreeFamiTF312990.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00328.
ReactomeiREACT_240936. Tryptophan catabolism.

Miscellaneous databases

NextBioi353386.
PROiQ91WN4.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WN4.
ExpressionAtlasiQ91WN4. baseline and differential.
GenevestigatoriQ91WN4.

Family and domain databases

HAMAPiMF_01971. Kynurenine_monooxygenase.
InterProiIPR027545. Kynurenine_monooxygenase.
IPR002938. mOase_FAD-bd.
IPR003042. Rng_hydrolase-like.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
PRINTSiPR00420. RNGMNOXGNASE.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/NImported.
    Tissue: LiverImported.

Entry informationi

Entry nameiKMO_MOUSE
AccessioniPrimary (citable) accession number: Q91WN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 1, 2001
Last modified: February 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.