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Protein

Ankyrin repeat domain-containing protein 54

Gene

Ankrd54

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation.1 Publication

GO - Molecular functioni

  • protein complex binding Source: MGI
  • protein kinase regulator activity Source: MGI

GO - Biological processi

  • nucleocytoplasmic transport Source: MGI
  • positive regulation of erythrocyte differentiation Source: MGI
  • regulation of intracellular signal transduction Source: MGI
  • regulation of protein kinase activity Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein 54
Alternative name(s):
Lyn-interacting ankyrin repeat protein
Gene namesi
Name:Ankrd54
Synonyms:Liar
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2444209. Ankrd54.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Midbody 1 Publication

  • Note: Shuttles between nucleus and cytoplasm during the cell cycle. EPO stimulation induces nuclear accumulation.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • midbody Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi98 – 981R → A: Increases the cytoplasmic protein content; when associated with A-99. 1 Publication
Mutagenesisi99 – 991R → A: Increases the cytoplasmic protein content; when associated with A-98. 1 Publication
Mutagenesisi289 – 2891L → A: Accumulation within the nucleus; when associated with A-291. 1 Publication
Mutagenesisi291 – 2911L → A: Accumulation within the nucleus; when associated with A-289. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 299298Ankyrin repeat domain-containing protein 54PRO_0000274494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei62 – 621PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ91WK7.
MaxQBiQ91WK7.
PaxDbiQ91WK7.
PRIDEiQ91WK7.

PTM databases

iPTMnetiQ91WK7.
PhosphoSiteiQ91WK7.

Expressioni

Tissue specificityi

Expressed in a variety of hemopoietic cell lines and tissue with high levels in testis. Highly expressed in ciliated cells.2 Publications

Developmental stagei

Expressed in brachial arches, maxillary process, fore and hind limb buds and in the developing gastrointestinal tract of day 10 embryos.

Gene expression databases

BgeeiENSMUSG00000033055.
CleanExiMM_ANKRD54.
ExpressionAtlasiQ91WK7. baseline and differential.
GenevisibleiQ91WK7. MM.

Interactioni

Subunit structurei

Interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN. Forms a multiprotein complex with LYN and HCLS1. Interacts with TSN2, VAV1, DBNL AND LASP1.1 Publication

GO - Molecular functioni

  • protein complex binding Source: MGI

Protein-protein interaction databases

IntActiQ91WK7. 2 interactions.
STRINGi10090.ENSMUSP00000047042.

Structurei

3D structure databases

ProteinModelPortaliQ91WK7.
SMRiQ91WK7. Positions 25-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati108 – 13730ANK 1Add
BLAST
Repeati141 – 17030ANK 2Add
BLAST
Repeati174 – 20330ANK 3Add
BLAST
Repeati207 – 23933ANK 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 240101LYN-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi98 – 11619Nuclear localization signal (NLS)Add
BLAST
Motifi282 – 29211Nuclear export signal (NES)Add
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129743.
HOGENOMiHOG000008674.
HOVERGENiHBG080858.
InParanoidiQ91WK7.
OMAiDDKELRY.
OrthoDBiEOG091G0WXS.
PhylomeDBiQ91WK7.
TreeFamiTF330790.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATGGGADD ESRSGRSSSD GECAVAPEPL AEAGGLVSFA DFGVSLGSGA
60 70 80 90 100
GLPGRSVGRA QSSLRYLQVL WQQDVEPRDE LRCKIPAGRL RRAARPHRRL
110 120 130 140 150
GPTGKEVHAL KRLRDSANAN DVETVQQLLE DGADPCAADD KGRTALHFAS
160 170 180 190 200
CNGNDQIVQL LLDHGADPNQ QDGLGNTPLH LAACTNHVPV ITTLLRGGAR
210 220 230 240 250
VDALDRAGRT PLHLAKSKLN ILQEGHSQCL EAVRLEVKQI IHMLREYLER
260 270 280 290
LGRHEQRERL DDLCTRLQMT STKEQVDEVT DLLASFTSLS LQMQSMEKR
Length:299
Mass (Da):32,486
Last modified:December 1, 2001 - v1
Checksum:i88E91A10F1953AC3
GO

Sequence cautioni

The sequence BAE25471 differs from that shown. Reason: Frameshift at position 291. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731G → N in BAE21840 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK133785 mRNA. Translation: BAE21840.1.
AK143625 mRNA. Translation: BAE25471.1. Frameshift.
BC014743 mRNA. Translation: AAH14743.1.
CCDSiCCDS37139.1.
RefSeqiNP_659098.1. NM_144849.1.
UniGeneiMm.24262.

Genome annotation databases

EnsembliENSMUST00000040676; ENSMUSP00000047042; ENSMUSG00000033055.
GeneIDi223690.
KEGGimmu:223690.
UCSCiuc007wsi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK133785 mRNA. Translation: BAE21840.1.
AK143625 mRNA. Translation: BAE25471.1. Frameshift.
BC014743 mRNA. Translation: AAH14743.1.
CCDSiCCDS37139.1.
RefSeqiNP_659098.1. NM_144849.1.
UniGeneiMm.24262.

3D structure databases

ProteinModelPortaliQ91WK7.
SMRiQ91WK7. Positions 25-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91WK7. 2 interactions.
STRINGi10090.ENSMUSP00000047042.

PTM databases

iPTMnetiQ91WK7.
PhosphoSiteiQ91WK7.

Proteomic databases

EPDiQ91WK7.
MaxQBiQ91WK7.
PaxDbiQ91WK7.
PRIDEiQ91WK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040676; ENSMUSP00000047042; ENSMUSG00000033055.
GeneIDi223690.
KEGGimmu:223690.
UCSCiuc007wsi.1. mouse.

Organism-specific databases

CTDi129138.
MGIiMGI:2444209. Ankrd54.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129743.
HOGENOMiHOG000008674.
HOVERGENiHBG080858.
InParanoidiQ91WK7.
OMAiDDKELRY.
OrthoDBiEOG091G0WXS.
PhylomeDBiQ91WK7.
TreeFamiTF330790.

Miscellaneous databases

PROiQ91WK7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033055.
CleanExiMM_ANKRD54.
ExpressionAtlasiQ91WK7. baseline and differential.
GenevisibleiQ91WK7. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANR54_MOUSE
AccessioniPrimary (citable) accession number: Q91WK7
Secondary accession number(s): Q3UPC5, Q3UZL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.