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Protein

Leucine-rich repeat flightless-interacting protein 2

Gene

Lrrfip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding (By similarity).By similarity

GO - Biological processi

  • MyD88-dependent toll-like receptor 4 signaling pathway Source: MGI
  • MyD88-independent toll-like receptor signaling pathway Source: MGI
  • positive regulation of NF-kappaB transcription factor activity Source: MGI
  • positive regulation of tumor necrosis factor secretion Source: MGI
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat flightless-interacting protein 2
Short name:
LRR FLII-interacting protein 2
Gene namesi
Name:Lrrfip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1918518. Lrrfip2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Leucine-rich repeat flightless-interacting protein 2PRO_0000245247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei95 – 951PhosphoserineBy similarity
Modified residuei103 – 1031PhosphoserineBy similarity
Modified residuei107 – 1071PhosphoserineBy similarity
Modified residuei111 – 1111PhosphoserineCombined sources
Modified residuei114 – 1141PhosphothreonineCombined sources
Modified residuei115 – 1151PhosphoserineCombined sources
Modified residuei116 – 1161PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91WK0.
MaxQBiQ91WK0.
PaxDbiQ91WK0.
PeptideAtlasiQ91WK0.
PRIDEiQ91WK0.

PTM databases

iPTMnetiQ91WK0.

Expressioni

Gene expression databases

BgeeiQ91WK0.

Interactioni

Subunit structurei

Interacts with DVL3 and FLII (By similarity). Weakly interacts with MYD88 in resting cells. Following LPS-stimulation, the interaction with MYD88 is rapidly enhanced; the complex gradually dissociates to basal levels after 6 hours of stimulation. Interaction with MYD88 is regulated by LPS-induced phosphorylation. In the presence of LPS, competes with FLII for MYD88-binding (By similarity).By similarity

Protein-protein interaction databases

BioGridi214592. 3 interactions.
IntActiQ91WK0. 2 interactions.
MINTiMINT-5102469.
STRINGi10090.ENSMUSP00000035078.

Structurei

3D structure databases

ProteinModelPortaliQ91WK0.
SMRiQ91WK0. Positions 134-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili22 – 4928Sequence analysisAdd
BLAST
Coiled coili121 – 21797Sequence analysisAdd
BLAST
Coiled coili260 – 408149Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the LRRFIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2010. Eukaryota.
ENOG4111H1S. LUCA.
HOGENOMiHOG000294125.
HOVERGENiHBG061558.
InParanoidiQ91WK0.
PhylomeDBiQ91WK0.

Family and domain databases

InterProiIPR019139. LRRFIP1/2.
[Graphical view]
PANTHERiPTHR19212. PTHR19212. 1 hit.
PfamiPF09738. DUF2051. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTPGSGRKR TPVKDRFSAE DEALSNIARE AEARLAAKRA ARAEARDIRM
60 70 80 90 100
RELERQQREG VEDTLSLRSL GSHRLDEKSD KQYAENYTRP SSRNSASATT
110 120 130 140 150
PLSGQSSRRG SGDTSSLIDP DTSLSELRES LSEVEEKYKK AMVSNAQLDN
160 170 180 190 200
EKNNLIYQVD TLKDVIEEQE EQMAEFYREN EEKSKELERQ KHMCSVLQHK
210 220 230 240 250
MDELKEGLRQ RDELIEKHGL VIIPDSTPNG DVHHEPVVGA ITAVSQEAAQ
260 270 280 290 300
VLESAGEGPL DVRLRKLAGE KDELLSQIRK LKLQLEEERQ KCSRNDGMSG
310 320 330 340 350
DLAGLQNGSD LQFIEMQRDA NRQISEYKFK LSKAEQDIAT LEQSISRLEG
360 370 380 390 400
QVLRYKTAAE NAEKIEDELK AERRKLQREL RTAQDKIEEM EMTNSHLAKR
410
LEKMKANRTA LLAQQ
Length:415
Mass (Da):47,148
Last modified:December 1, 2001 - v1
Checksum:i9A877ACA5A7264B8
GO
Isoform 2 (identifier: Q91WK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-74: Missing.

Show »
Length:400
Mass (Da):45,539
Checksum:i592F6A0CC834C68A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051Q → N in BAC37441 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei60 – 7415Missing in isoform 2. 1 PublicationVSP_019678Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017217 mRNA. Translation: BAB30640.1.
AK078881 mRNA. Translation: BAC37441.1.
BC014761 mRNA. Translation: AAH14761.1.
CCDSiCCDS23583.1. [Q91WK0-2]
CCDS52941.1. [Q91WK0-1]
RefSeqiNP_001158310.1. NM_001164838.1.
NP_082018.1. NM_027742.3.
UniGeneiMm.302287.

Genome annotation databases

GeneIDi71268.
KEGGimmu:71268.
UCSCiuc009rvm.2. mouse. [Q91WK0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017217 mRNA. Translation: BAB30640.1.
AK078881 mRNA. Translation: BAC37441.1.
BC014761 mRNA. Translation: AAH14761.1.
CCDSiCCDS23583.1. [Q91WK0-2]
CCDS52941.1. [Q91WK0-1]
RefSeqiNP_001158310.1. NM_001164838.1.
NP_082018.1. NM_027742.3.
UniGeneiMm.302287.

3D structure databases

ProteinModelPortaliQ91WK0.
SMRiQ91WK0. Positions 134-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214592. 3 interactions.
IntActiQ91WK0. 2 interactions.
MINTiMINT-5102469.
STRINGi10090.ENSMUSP00000035078.

PTM databases

iPTMnetiQ91WK0.

Proteomic databases

EPDiQ91WK0.
MaxQBiQ91WK0.
PaxDbiQ91WK0.
PeptideAtlasiQ91WK0.
PRIDEiQ91WK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi71268.
KEGGimmu:71268.
UCSCiuc009rvm.2. mouse. [Q91WK0-2]

Organism-specific databases

CTDi9209.
MGIiMGI:1918518. Lrrfip2.

Phylogenomic databases

eggNOGiKOG2010. Eukaryota.
ENOG4111H1S. LUCA.
HOGENOMiHOG000294125.
HOVERGENiHBG061558.
InParanoidiQ91WK0.
PhylomeDBiQ91WK0.

Miscellaneous databases

ChiTaRSiLrrfip2. mouse.
PROiQ91WK0.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WK0.

Family and domain databases

InterProiIPR019139. LRRFIP1/2.
[Graphical view]
PANTHERiPTHR19212. PTHR19212. 1 hit.
PfamiPF09738. DUF2051. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Colon, Intestine and Olfactory bulb.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-111; THR-114; SER-115 AND SER-116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiLRRF2_MOUSE
AccessioniPrimary (citable) accession number: Q91WK0
Secondary accession number(s): Q8BVD1, Q9CU89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.