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Protein

Far upstream element-binding protein 1

Gene

Fubp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell growth Source: MGI
  • hematopoietic stem cell proliferation Source: MGI
  • positive regulation of gene expression Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • stem cell division Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Far upstream element-binding protein 1
Short name:
FBP
Short name:
FUSE-binding protein 1
Gene namesi
Name:Fubp1
Synonyms:D3Ertd330e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1196294. Fubp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000501362 – 651Far upstream element-binding protein 1Add BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei149PhosphothreonineCombined sources1
Modified residuei317Omega-N-methylarginineCombined sources1
Modified residuei355Omega-N-methylarginineCombined sources1
Modified residuei357Omega-N-methylarginineCombined sources1
Modified residuei359Omega-N-methylarginineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei428PhosphothreonineBy similarity1
Modified residuei626PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. This targets the protein for proteasome-mediated degradation (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91WJ8.
PaxDbiQ91WJ8.
PeptideAtlasiQ91WJ8.
PRIDEiQ91WJ8.

2D gel databases

REPRODUCTION-2DPAGEQ91WJ8.

PTM databases

iPTMnetiQ91WJ8.
PhosphoSitePlusiQ91WJ8.
SwissPalmiQ91WJ8.

Expressioni

Gene expression databases

CleanExiMM_FUBP1.
ExpressionAtlasiQ91WJ8. baseline and differential.
GenevisibleiQ91WJ8. MM.

Interactioni

Subunit structurei

Found in a complex with PUF60 and far upstream element (FUSE) DNA segment. Interacts with PUF60 and JTV1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi206245. 1 interactor.
IntActiQ91WJ8. 3 interactors.
MINTiMINT-1854064.
STRINGi10090.ENSMUSP00000101727.

Structurei

Secondary structure

1651
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi97 – 104Combined sources8
Helixi105 – 112Combined sources8
Beta strandi114 – 116Combined sources3
Helixi117 – 125Combined sources9
Beta strandi129 – 132Combined sources4
Beta strandi140 – 148Combined sources9
Helixi150 – 166Combined sources17
Beta strandi182 – 188Combined sources7
Helixi190 – 196Combined sources7
Beta strandi199 – 202Combined sources4
Helixi203 – 211Combined sources9
Beta strandi213 – 217Combined sources5
Beta strandi229 – 235Combined sources7
Turni237 – 239Combined sources3
Helixi240 – 251Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4MNMR-A175-255[»]
1X4NNMR-A91-168[»]
ProteinModelPortaliQ91WJ8.
SMRiQ91WJ8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91WJ8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 160KH 1PROSITE-ProRule annotationAdd BLAST65
Domaini181 – 247KH 2PROSITE-ProRule annotationAdd BLAST67
Domaini271 – 335KH 3PROSITE-ProRule annotationAdd BLAST65
Domaini372 – 439KH 4PROSITE-ProRule annotationAdd BLAST68

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 21Poly-Gly6
Compositional biasi345 – 392Gly-richAdd BLAST48
Compositional biasi446 – 556Pro-richAdd BLAST111

Sequence similaritiesi

Contains 4 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
GeneTreeiENSGT00730000110664.
HOGENOMiHOG000231552.
HOVERGENiHBG000625.
InParanoidiQ91WJ8.
PhylomeDBiQ91WJ8.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADYSTVPPP SSGSAGGGGG GVVNDAFKDA LQRARQIAAK IGGDAGTSLN
60 70 80 90 100
SNDYGYGGQK RPLEDGDQPD AKKVPPQNDS FGAQLPPMHQ QQSRSVMTEE
110 120 130 140 150
YKVPDGMVGF IIGRGGEQIS RIQQESGCKI QIAPDSGGLP ERSCMLTGTP
160 170 180 190 200
ESVQSAKRLL DQIVEKGRPA PGFHHGDGPG NAVQEIMIPA SKAGLVIGKG
210 220 230 240 250
GETIKQLQER AGVKMVMIQD GPQNTGADKP LRITGDPYKV QQAKEMVLEL
260 270 280 290 300
IRDQGGFREV RNEYGSRIGG NEGIDVPIPR FAVGIVIGRN GEMIKKIQND
310 320 330 340 350
AGVRIQFKPD DGTTPDRIAQ ITGPPDRCQH AAEIITDLLR SVQAGNPGGP
360 370 380 390 400
GPGGRGRGRG QGNWNMGPPG GLQEFNFIVP TGKTGLIIGK GGETIKSISQ
410 420 430 440 450
QSGARIELQR SPPPNADPNM KLFTIRGTPQ QIDYARQLIE EKIGGPVNPL
460 470 480 490 500
GPPVPHGPHG VPGPHGPPGP PGPGTPMGPY NPAPYNPGPP GPAPHGPPAP
510 520 530 540 550
YAPQGWGNAY PHWQQQAPPD PAKAGADPNS AAWAAYYAHY YQQQAQPPPA
560 570 580 590 600
APAGAPATTQ TNGQGDQQAP APAGQVDYTK AWEEYYKKMG QAVPAPAGAP
610 620 630 640 650
PGGQPDYSAA WAEYYRQQAA YYAQTSPQGM PQHPPAPQGF ANHARSHHHL

Y
Length:651
Mass (Da):68,540
Last modified:December 1, 2001 - v1
Checksum:iF4EECA62FD9FA0D5
GO
Isoform 2 (identifier: Q91WJ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-67: D → DGSWTNPSSTTHWEGMPSPFKD

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):70,871
Checksum:i430D5A827D695D46
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93Missing in BAC26457 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00832267D → DGSWTNPSSTTHWEGMPSPF KD in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014763 mRNA. Translation: AAH14763.1.
AK029458 mRNA. Translation: BAC26457.1.
RefSeqiXP_006501734.1. XM_006501671.1. [Q91WJ8-1]
UniGeneiMm.278922.

Genome annotation databases

EnsembliENSMUST00000196739; ENSMUSP00000143101; ENSMUSG00000028034. [Q91WJ8-1]
GeneIDi51886.
UCSCiuc008rsv.1. mouse. [Q91WJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014763 mRNA. Translation: AAH14763.1.
AK029458 mRNA. Translation: BAC26457.1.
RefSeqiXP_006501734.1. XM_006501671.1. [Q91WJ8-1]
UniGeneiMm.278922.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4MNMR-A175-255[»]
1X4NNMR-A91-168[»]
ProteinModelPortaliQ91WJ8.
SMRiQ91WJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206245. 1 interactor.
IntActiQ91WJ8. 3 interactors.
MINTiMINT-1854064.
STRINGi10090.ENSMUSP00000101727.

PTM databases

iPTMnetiQ91WJ8.
PhosphoSitePlusiQ91WJ8.
SwissPalmiQ91WJ8.

2D gel databases

REPRODUCTION-2DPAGEQ91WJ8.

Proteomic databases

EPDiQ91WJ8.
PaxDbiQ91WJ8.
PeptideAtlasiQ91WJ8.
PRIDEiQ91WJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000196739; ENSMUSP00000143101; ENSMUSG00000028034. [Q91WJ8-1]
GeneIDi51886.
UCSCiuc008rsv.1. mouse. [Q91WJ8-1]

Organism-specific databases

CTDi8880.
MGIiMGI:1196294. Fubp1.

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
GeneTreeiENSGT00730000110664.
HOGENOMiHOG000231552.
HOVERGENiHBG000625.
InParanoidiQ91WJ8.
PhylomeDBiQ91WJ8.

Miscellaneous databases

EvolutionaryTraceiQ91WJ8.
PROiQ91WJ8.
SOURCEiSearch...

Gene expression databases

CleanExiMM_FUBP1.
ExpressionAtlasiQ91WJ8. baseline and differential.
GenevisibleiQ91WJ8. MM.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUBP1_MOUSE
AccessioniPrimary (citable) accession number: Q91WJ8
Secondary accession number(s): Q8C0Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.