Reviewed, UniProtKB/Swiss-Prot Q91WG7 (DGKG_MOUSE)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase gamma
      Short name=DAG kinase gamma
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase gamma
      Short name=DGK-gamma
    88 kDa diacylglycerol kinase

Gene names

Name:	Dgkg
Synonyms:	Dagk3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 788	788	Diacylglycerol kinase gamma		PRO_0000218460
Regions
	Domain	172 – 207	36	EF-hand 1
	Domain	217 – 252	36	EF-hand 2
	Domain	427 – 561	135	DAGKc
	Calcium binding	185 – 196	12	1 Potential
	Calcium binding	230 – 241	12	2 Potential
	Zinc finger	268 – 318	51	Phorbol-ester/DAG-type 1
	Zinc finger	333 – 380	48	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	Q91WG7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 969298DFD144F5DF	FASTA	788	88,523
	10 20 30 40 50 60 MSEEQWVSLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LRQYDPHKPI SYDVFKLFMR 70 80 90 100 110 120 AYLEVDLPQP LSTHLFLAFS QKPRQETPDH PKEGASSSEP NVSDYNSDNA AKADEACAPD 130 140 150 160 170 180 TESKTTKTQA PSKELEAAAP WEDPGALASS SDAPVVYLKD VVCYLSLMET GRPQDKLEFM 190 200 210 220 230 240 FRLYDSDENG LLDQAEMDQI VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YDKDGFVSLQ 250 260 270 280 290 300 EWINGGMTTI PLLVLLGMDD SGSKGDGRHA WTLKHFKKPT YCNFCRAMLM GVGKQGLCCI 310 320 330 340 350 360 YCKYTVHQRC VSKTIHGCVK TNSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA 370 380 390 400 410 420 RHCVWCRMTF HRKCELSTVC DGGELKDHIL LPTSICPVSG DRQGGKSDGS VAAKGELVTQ 430 440 450 460 470 480 YKIIPSPGTH PLLVLVNPKS GGRQGERILR KFHYLLNPEQ VFNLDNGGPT PGLNFFHDTP 490 500 510 520 530 540 DFRVLACGGD GTVGWILDCI DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI 550 560 570 580 590 600 LKEIEQSPLV MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHMMREKHP 610 620 630 640 650 660 EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE GIAILNIPSM 670 680 690 700 710 720 YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ LLEVVGLEGA MEMGQIYTGL 730 740 750 760 770 780 KSAGRRLAQC SSVTIRTNKL LPMQVDGEPW MQPQCTIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC015278 mRNA. Translation: AAH15278.1.
IPI	IPI00128127.
RefSeq	NP_619591.1.
UniGene	Mm.194986 Mm.448158
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q91WG7.
PTM databases
PhosphoSite	Q91WG7.
Proteomic databases
PRIDE	Q91WG7.
Genome annotation databases
Ensembl	ENSMUST00000089925; ENSMUSP00000087371; ENSMUSG00000022861; Mus musculus. [Genome view]
GeneID	110197.
KEGG	mmu:110197.
UCSC	uc007ysf.1. mouse.
Organism-specific databases
CTD	110197.
MGI	MGI:105060. Dgkg.
Phylogenomic databases
HOVERGEN	Q91WG7.
OMA	EVMQHAW.
Enzyme and pathway databases
BRENDA	2.7.1.107. 244.
Gene expression databases
ArrayExpress	Q91WG7.
Bgee	Q91WG7.
CleanEx	MM_DGKG.
Genevestigator	Q91WG7.
GermOnline	ENSMUSG00000022861. Mus musculus.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	363515.
SOURCE	Search...

Entry information

Entry name

DGKG_MOUSE

Accession

Primary (citable) accession number: Q91WG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)