Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q91WG5

- AAKG2_MOUSE

UniProt

Q91WG5 - AAKG2_MOUSE

Protein

5'-AMP-activated protein kinase subunit gamma-2

Gene

Prkag2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei299 – 2991AMP 1By similarity
    Binding sitei299 – 2991ATP 1By similarity
    Binding sitei380 – 3801AMP 2By similarity
    Binding sitei380 – 3801AMP 3By similarity
    Binding sitei380 – 3801ATP 2By similarity
    Binding sitei381 – 3811ATP 1By similarity
    Binding sitei381 – 3811ATP 2By similarity
    Binding sitei399 – 3991AMP 1By similarity
    Binding sitei399 – 3991ATP 1By similarity
    Binding sitei527 – 5271AMP 3By similarity
    Binding sitei528 – 5281AMP 1By similarity
    Binding sitei528 – 5281ATP 1By similarity

    GO - Molecular functioni

    1. ADP binding Source: Ensembl
    2. AMP-activated protein kinase activity Source: MGI
    3. AMP binding Source: Ensembl
    4. ATP binding Source: UniProtKB-KW
    5. cAMP-dependent protein kinase inhibitor activity Source: Ensembl
    6. cAMP-dependent protein kinase regulator activity Source: Ensembl
    7. phosphorylase kinase regulator activity Source: Ensembl
    8. protein kinase activator activity Source: Ensembl

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW
    2. glycogen metabolic process Source: Ensembl
    3. intracellular signal transduction Source: Ensembl
    4. positive regulation of peptidyl-threonine phosphorylation Source: Ensembl
    5. protein phosphorylation Source: GOC
    6. regulation of fatty acid metabolic process Source: Ensembl
    7. regulation of glycolytic process Source: Ensembl

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205688. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
    REACT_220701. Regulation of AMPK activity via LKB1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5'-AMP-activated protein kinase subunit gamma-2
    Short name:
    AMPK gamma2
    Short name:
    AMPK subunit gamma-2
    Gene namesi
    Name:Prkag2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1336153. Prkag2.

    Subcellular locationi

    GO - Cellular componenti

    1. AMP-activated protein kinase complex Source: Ensembl
    2. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5665665'-AMP-activated protein kinase subunit gamma-2PRO_0000204382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei65 – 651Phosphoserine2 Publications
    Modified residuei71 – 711Phosphoserine2 Publications
    Modified residuei73 – 731Phosphoserine2 Publications
    Modified residuei90 – 901Phosphoserine2 Publications
    Modified residuei138 – 1381Phosphoserine2 Publications
    Modified residuei143 – 1431Phosphoserine2 Publications
    Modified residuei158 – 1581Phosphoserine2 Publications
    Modified residuei161 – 1611Phosphoserine2 Publications
    Modified residuei196 – 1961Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ91WG5.
    PaxDbiQ91WG5.
    PRIDEiQ91WG5.

    PTM databases

    PhosphoSiteiQ91WG5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91WG5.
    BgeeiQ91WG5.
    GenevestigatoriQ91WG5.

    Interactioni

    Subunit structurei

    AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi223832. 1 interaction.
    IntActiQ91WG5. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91WG5.
    SMRiQ91WG5. Positions 257-554.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini272 – 33261CBS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini354 – 41259CBS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini427 – 48963CBS 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini501 – 55959CBS 4PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi367 – 38822AMPK pseudosubstrateAdd
    BLAST

    Domaini

    The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1 By similarity.By similarity
    The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP By similarity.By similarity

    Sequence similaritiesi

    Contains 4 CBS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiCOG0517.
    GeneTreeiENSGT00390000009849.
    HOGENOMiHOG000176880.
    HOVERGENiHBG050431.
    InParanoidiQ91WG5.
    KOiK07200.
    OMAiGAKQKEN.
    OrthoDBiEOG74FF0W.
    TreeFamiTF313247.

    Family and domain databases

    InterProiIPR000644. CBS_dom.
    [Graphical view]
    PfamiPF00571. CBS. 3 hits.
    [Graphical view]
    SMARTiSM00116. CBS. 4 hits.
    [Graphical view]
    PROSITEiPS51371. CBS. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q91WG5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSAAMDTKK KKEVSSPGGS SGKKNPSLKR RSLRVHIPDL SSFAMPLLDG    50
    DVENSEKHSS RKVDSPFSSG SPSRGLFSRG PQPRPSSPVS APVRPKTSPG 100
    SPKTVFPFSY QESPPRSPRR MSFSGIFRSS SKESSPNSNP STSPGGIRFF 150
    SRSRKTSSVS SSPSTPTQVT KQHPFPLESY KQEPERPESR IYASSSPPDT 200
    GQRFCLAFQS PARPPLASPT YHAPLRTAVL AAAPGPAEAG MLEKLEFQEE 250
    EDSESGVYMR FMRSHKCYDI VPTSSKLVVF DTTLQVKKAF FALVANGVRA 300
    APLWESKKQS FVGMLTITDF INILHRYYKS PMVQIYELEE HKIETWRELY 350
    LQETFKPLVN ISPDASLFDA VYSLIKNKIH RLPVIDPISG NALYILTHKR 400
    ILKFLQLFMS DMPKPAFMKQ NLDELGIGTY HNIAFIHPDT PIIKALNIFV 450
    ERRISALPVV DESGKVVDIY SKFDVINLAA EKTYNNLDIT VTQALQHRSQ 500
    YFEGVVKCSK LETLETIVDR IVRAEVHRLV VVNEADSIVG IISLSDILQA 550
    LILTPAGAKQ KETETE 566
    Length:566
    Mass (Da):62,949
    Last modified:July 27, 2011 - v2
    Checksum:iD112BFD69D1C5ACB
    GO
    Isoform B (identifier: Q91WG5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-240: Missing.

    Show »
    Length:326
    Mass (Da):37,312
    Checksum:iFEA2EEAB9550999A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti257 – 2571V → F in AAH15283. (PubMed:15489334)Curated
    Sequence conflicti516 – 5161T → N in AAQ55224. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 240240Missing in isoform B. 1 PublicationVSP_015586Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY348864 mRNA. Translation: AAQ55224.1.
    AC116151 Genomic DNA. No translation available.
    AC125270 Genomic DNA. No translation available.
    BC015283 mRNA. Translation: AAH15283.1.
    CCDSiCCDS19130.1. [Q91WG5-1]
    CCDS51437.1. [Q91WG5-2]
    RefSeqiNP_001164027.1. NM_001170556.1. [Q91WG5-2]
    NP_663376.2. NM_145401.2. [Q91WG5-1]
    UniGeneiMm.33649.

    Genome annotation databases

    EnsembliENSMUST00000030784; ENSMUSP00000030784; ENSMUSG00000028944. [Q91WG5-1]
    ENSMUST00000114975; ENSMUSP00000110626; ENSMUSG00000028944. [Q91WG5-2]
    GeneIDi108099.
    KEGGimmu:108099.
    UCSCiuc008wsk.2. mouse. [Q91WG5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY348864 mRNA. Translation: AAQ55224.1 .
    AC116151 Genomic DNA. No translation available.
    AC125270 Genomic DNA. No translation available.
    BC015283 mRNA. Translation: AAH15283.1 .
    CCDSi CCDS19130.1. [Q91WG5-1 ]
    CCDS51437.1. [Q91WG5-2 ]
    RefSeqi NP_001164027.1. NM_001170556.1. [Q91WG5-2 ]
    NP_663376.2. NM_145401.2. [Q91WG5-1 ]
    UniGenei Mm.33649.

    3D structure databases

    ProteinModelPortali Q91WG5.
    SMRi Q91WG5. Positions 257-554.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223832. 1 interaction.
    IntActi Q91WG5. 1 interaction.

    PTM databases

    PhosphoSitei Q91WG5.

    Proteomic databases

    MaxQBi Q91WG5.
    PaxDbi Q91WG5.
    PRIDEi Q91WG5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030784 ; ENSMUSP00000030784 ; ENSMUSG00000028944 . [Q91WG5-1 ]
    ENSMUST00000114975 ; ENSMUSP00000110626 ; ENSMUSG00000028944 . [Q91WG5-2 ]
    GeneIDi 108099.
    KEGGi mmu:108099.
    UCSCi uc008wsk.2. mouse. [Q91WG5-1 ]

    Organism-specific databases

    CTDi 51422.
    MGIi MGI:1336153. Prkag2.

    Phylogenomic databases

    eggNOGi COG0517.
    GeneTreei ENSGT00390000009849.
    HOGENOMi HOG000176880.
    HOVERGENi HBG050431.
    InParanoidi Q91WG5.
    KOi K07200.
    OMAi GAKQKEN.
    OrthoDBi EOG74FF0W.
    TreeFami TF313247.

    Enzyme and pathway databases

    Reactomei REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_205688. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
    REACT_220701. Regulation of AMPK activity via LKB1.

    Miscellaneous databases

    ChiTaRSi PRKAG2. mouse.
    NextBioi 360062.
    PROi Q91WG5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91WG5.
    Bgeei Q91WG5.
    Genevestigatori Q91WG5.

    Family and domain databases

    InterProi IPR000644. CBS_dom.
    [Graphical view ]
    Pfami PF00571. CBS. 3 hits.
    [Graphical view ]
    SMARTi SM00116. CBS. 4 hits.
    [Graphical view ]
    PROSITEi PS51371. CBS. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of mouse protein kinase, AMP-activated, gamma 2 non-catalytic subunit."
      Zhou G., Jiang D., Zhang Y.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Strain: C57BL/6J.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: FVB/N.
      Tissue: Kidney.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-71; SER-73; SER-90; SER-138; SER-143; SER-158; SER-161 AND SER-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop."
      Loffler A.S., Alers S., Dieterle A.M., Keppeler H., Franz-Wachtel M., Kundu M., Campbell D.G., Wesselborg S., Alessi D.R., Stork B.
      Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY ULK1.

    Entry informationi

    Entry nameiAAKG2_MOUSE
    AccessioniPrimary (citable) accession number: Q91WG5
    Secondary accession number(s): E9QK80, Q6V7V5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3