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Protein

Acylcarnitine hydrolase

Gene

Ces2c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolase with high activity towards palmitoylcarnitine. Is also active with p-nitrophenylacetate and alpha-naphthylacetate.1 Publication

Catalytic activityi

O-acylcarnitine + H2O = a fatty acid + L-carnitine.1 Publication

Kineticsi

  1. KM=98.8 µM for palmitoyl-dl-carnitine
  2. KM=392 µM for p-nitrophenylacetate
  1. Vmax=1.67 µmol/min/mg enzyme with palmitoyl-dl-carnitine
  2. Vmax=353 µmol/min/mg enzyme with p-nitrophenylacetate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei230 – 2301Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei347 – 3471Charge relay systemBy similarity
Active sitei459 – 4591Charge relay systemBy similarity

GO - Molecular functioni

  • acylcarnitine hydrolase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Protein family/group databases

ESTHERimouse-Ces2c. Carb_B_Chordata.
MEROPSiS09.999.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylcarnitine hydrolase (EC:3.1.1.28)
Short name:
ACH M1
Alternative name(s):
Carboxylesterase 2
Short name:
CES 2
Peroxisome proliferator-inducible acylcarnitine hydrolase
Gene namesi
Name:Ces2c
Synonyms:Ces2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2385905. Ces2c.

Subcellular locationi

  • Microsome 1 Publication
  • Endoplasmic reticulum Curated

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • extracellular space Source: GO_Central
  • intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 561535Acylcarnitine hydrolasePRO_5000396880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Pyrrolidone carboxylic acidBy similarity
Disulfide bondi97 ↔ 125By similarity
Disulfide bondi282 ↔ 293By similarity
Modified residuei554 – 5541PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

EPDiQ91WG0.
MaxQBiQ91WG0.
PaxDbiQ91WG0.
PeptideAtlasiQ91WG0.
PRIDEiQ91WG0.

PTM databases

iPTMnetiQ91WG0.

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Inductioni

Up-regulated in liver by di-(2-ethylhexyl)phtalate (DEHP).1 Publication

Gene expression databases

BgeeiQ91WG0.
GenevisibleiQ91WG0. MM.

Interactioni

Protein-protein interaction databases

IntActiQ91WG0. 4 interactions.
STRINGi10090.ENSMUSP00000058567.

Chemistry

BindingDBiQ91WG0.

Structurei

3D structure databases

ProteinModelPortaliQ91WG0.
SMRiQ91WG0. Positions 34-541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi558 – 5614Prevents secretion from ERSequence analysis

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ91WG0.
KOiK03927.
OMAiFERGDIN.
OrthoDBiEOG7RBZ7R.
PhylomeDBiQ91WG0.
TreeFamiTF315470.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRNQLHNWL NAGFFGLLLL LIHVQGQDSP EANPIRNTHT GQIQGSLIHV
60 70 80 90 100
KDTKAGVHTF LGIPFAKPPV GPLRFAPPEA PEPWSGVRDG TAHPAMCLQN
110 120 130 140 150
LDMLNEAGLP DMKMMLSSFP MSEDCLYLNI YTPAHAHEGS NLPVMVWIHG
160 170 180 190 200
GALVIGMASM FDGSLLTVNE DLVVVTIQYR LGVLGFFSTG DQHARGNWGY
210 220 230 240 250
LDQAAALRWV QQNIAHFGGN PDRVTIFGES AGGTSVSSHV VSPMSQGLFH
260 270 280 290 300
GAIMESGVAL LPDLISETSE MVSTTVAKLS GCEAMDSQAL VRCLRGKSEA
310 320 330 340 350
EILAINKVFK MIPAVVDGEF FPRHPKELLA SEDFHPVPSI IGVNNDEFGW
360 370 380 390 400
SIPVVMGSAQ MIKGITRENL QAVLKDTAVQ MMLPPECSDL LMEEYMGDTE
410 420 430 440 450
DAQTLQIQFT EMMGDFMFVI PALQVAHFQR SHAPVYFYEF QHPPSYFKDV
460 470 480 490 500
RPPHVKADHA DEIPFVFASF FWGMKLDFTE EEELLSRRMM KYWANFARHG
510 520 530 540 550
NPNSEGLPYW PVMDHDEQYL QLDIQPAVGR ALKAGRLQFW TKTLPQKIQE
560
LKASQDKHRE L
Length:561
Mass (Da):62,470
Last modified:December 1, 2001 - v1
Checksum:iF793967A646EB728
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110073 mRNA. Translation: BAC76623.1.
AC156564 Genomic DNA. No translation available.
BC015290 mRNA. Translation: AAH15290.1.
BC024552 mRNA. Translation: AAH24552.1.
BC031170 mRNA. Translation: AAH31170.1.
BC034178 mRNA. Translation: AAH34178.1.
BC034180 mRNA. Translation: AAH34180.1.
BC034191 mRNA. Translation: AAH34191.1.
CCDSiCCDS22587.1.
RefSeqiNP_663578.1. NM_145603.2.
UniGeneiMm.28191.

Genome annotation databases

EnsembliENSMUST00000055052; ENSMUSP00000058567; ENSMUSG00000061825.
GeneIDi234671.
KEGGimmu:234671.
UCSCiuc009nbe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110073 mRNA. Translation: BAC76623.1.
AC156564 Genomic DNA. No translation available.
BC015290 mRNA. Translation: AAH15290.1.
BC024552 mRNA. Translation: AAH24552.1.
BC031170 mRNA. Translation: AAH31170.1.
BC034178 mRNA. Translation: AAH34178.1.
BC034180 mRNA. Translation: AAH34180.1.
BC034191 mRNA. Translation: AAH34191.1.
CCDSiCCDS22587.1.
RefSeqiNP_663578.1. NM_145603.2.
UniGeneiMm.28191.

3D structure databases

ProteinModelPortaliQ91WG0.
SMRiQ91WG0. Positions 34-541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91WG0. 4 interactions.
STRINGi10090.ENSMUSP00000058567.

Chemistry

BindingDBiQ91WG0.
ChEMBLiCHEMBL2217.

Protein family/group databases

ESTHERimouse-Ces2c. Carb_B_Chordata.
MEROPSiS09.999.

PTM databases

iPTMnetiQ91WG0.

Proteomic databases

EPDiQ91WG0.
MaxQBiQ91WG0.
PaxDbiQ91WG0.
PeptideAtlasiQ91WG0.
PRIDEiQ91WG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055052; ENSMUSP00000058567; ENSMUSG00000061825.
GeneIDi234671.
KEGGimmu:234671.
UCSCiuc009nbe.2. mouse.

Organism-specific databases

CTDi234671.
MGIiMGI:2385905. Ces2c.

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ91WG0.
KOiK03927.
OMAiFERGDIN.
OrthoDBiEOG7RBZ7R.
PhylomeDBiQ91WG0.
TreeFamiTF315470.

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Miscellaneous databases

PROiQ91WG0.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WG0.
GenevisibleiQ91WG0. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, molecular cloning, and functional expression of inducible liver acylcarnitine hydrolase in C57BL/6 mouse, belonging to the carboxylesterase multigene family."
    Furihata T., Hosokawa M., Nakata F., Satoh T., Chiba K.
    Arch. Biochem. Biophys. 416:101-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CATALYTIC ACTIVITY, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiEST2C_MOUSE
AccessioniPrimary (citable) accession number: Q91WG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.