Reviewed, UniProtKB/Swiss-Prot Q91WF3 (ADCY4_MOUSE)

Names and origin

Protein names

Recommended name:
    Adenylate cyclase type 4
    EC=4.6.1.1
Alternative name(s):
    Adenylate cyclase type IV
    ATP pyrophosphate-lyase 4
    Adenylyl cyclase 4

Gene names

Name:

Adcy4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a membrane-bound, calmodulin-insensitive adenylyl cyclase By similarity.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Insensitive to calcium/calmodulin. Stimulated by the G protein beta and gamma subunit complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cAMP nucleotide second messenger Inferred from direct assay. Source: MGI activation of adenylate cyclase activity Traceable author statement. Source: MGI cAMP biosynthetic process Traceable author statement. Source: MGI
Cellular component	dendrite Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from direct assay. Source: MGI plasma membrane Traceable author statement. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Traceable author statement. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Adenylate cyclase type 4		PRO_0000195691
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 50	22	Potential
	Transmembrane	61 – 80	20	Potential
	Transmembrane	94 – 117	24	Potential
	Transmembrane	120 – 138	19	Potential
	Transmembrane	141 – 162	22	Potential
	Transmembrane	170 – 190	21	Potential
	Topological domain	191 – 582	392	Cytoplasmic Potential
	Transmembrane	583 – 604	22	Potential
	Transmembrane	608 – 630	23	Potential
	Transmembrane	661 – 684	24	Potential
	Topological domain	685 – 717	33	Extracellular Potential
	Transmembrane	718 – 738	21	Potential
	Transmembrane	746 – 766	21	Potential
	Transmembrane	793 – 809	17	Potential
	Topological domain	810 – 1077	268	Cytoplasmic Potential
Sites
	Metal binding	278	1	Magnesium 1 By similarity
	Metal binding	278	1	Magnesium 2 By similarity
	Metal binding	279	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	322	1	Magnesium 1 By similarity
	Metal binding	322	1	Magnesium 2 By similarity
Amino acid modifications
	Glycosylation	694	1	N-linked (GlcNAc...) Potential
	Glycosylation	701	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q91WF3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 03EA262D05F4B36D	FASTA	1,077	120,380
	10 20 30 40 50 60 MARLFSPRPP PSEDLFYETY YSLSQQYPLL ILLLVIVLCA LVALPAVAWA SGRELTSDPS 70 80 90 100 110 120 FLTTVLCALG GFSLLLGLAS REQQLQRWTR PLSGLIWVAL LALGYGFLFT GGVVSAWDQV 130 140 150 160 170 180 SFFLFIIFTV YAMLPLGMRD AAAAGVISSL SHLLVLGLYL GWQPESQRAL LPQLAANAVL 190 200 210 220 230 240 FLCGNVVGAY HKALMERALR ATFREALSSL HSRRRLDTEK KHQEHLLLSI LPAYLAREMK 250 260 270 280 290 300 AEIMARLQAG QRSRPENTNN FHSLYVKRHQ GVSVLYADIV GFTRLASECS PKELVLMLNE 310 320 330 340 350 360 LFGKFDQIAK EHECMRIKIL GDCYYCVSGL PLSLPDHAIN CVRMGLDMCR AIRKLRVATG 370 380 390 400 410 420 VDINMRVGVH SGSVLCGVIG LQKWQYDVWS HDVTLANHME AGGVPGRVHI TGATLALLAG 430 440 450 460 470 480 AYAVERADTE HRDPYLRELG EPTYLVIDPR AEEEDEKGTA KGLLSSLEGH TMRPSLLMTR 490 500 510 520 530 540 YLESWGAAKP FAHLSHLDSP VSTSTPLPEK AFSPQWSLDR SRTPRGLDDE LDTGDAKFFQ 550 560 570 580 590 600 VIEQLNSQKQ WKQSKDFNLL TLYFREKEME KQYRLSALPA FKYYAACTFL VFLSNFTIQM 610 620 630 640 650 660 LVTTRPPALI ITYSITFLLF FLLLFVCFSE HLTKCVQKGP KMLHWLPALS VLVATRPGFR 670 680 690 700 710 720 VALGTATILL VFTMAIASLL FLPVSSDCLF LASNVSSVTF NASWEMPGSL PLISIPLISI 730 740 750 760 770 780 PYSMHCCVLG FLSCSLFLHM SFELKLLLLL LWLVASCSLF LHSHAWLSDC LIARLYQSPS 790 800 810 820 830 840 DSRPGVLKEP KLMGAIYFFI FFFTLLVLAR QNEYYCRLDF LWKKKLRQER EETETMENLT 850 860 870 880 890 900 RLLLENVLPA HVAPQFIGQN RRNEDLYHQS YECVCVLFAS VPDFKEFYSE SNINHEGLEC 910 920 930 940 950 960 LRLLNEIIAD FDELLSKPKF SGVEKIKTIG STYMAATGLN ATSGQDTQQD SERSCSHLGT 970 980 990 1000 1010 1020 MVEFAVALGS KLGVINKHSF NNFRLRVGLN HGPVVAGVIG AQKPQYDIWG NTVNVASRME 1030 1040 1050 1060 1070 STGVLGKIQV TEETARALQS LGYTCYSRGS IKVKGKGELC TYFLNTDLTR TGSPSAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of mouse adenylyl cyclase type IV." Rui X., Schimmer B.P. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FVB/N. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 355-366, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF442771 mRNA. Translation: AAL38004.1. BC015299 mRNA. Translation: AAH15299.1.
IPI	IPI00128097.
RefSeq	NP_536683.1.
UniGene	Mm.287010
3D structure databases
SMR	Q91WF3. Positions 261-447, 867-1066.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91WF3.
PTM databases
PhosphoSite	Q91WF3.
Proteomic databases
PRIDE	Q91WF3.
Genome annotation databases
Ensembl	ENSMUST00000002398; ENSMUSP00000002398; ENSMUSG00000022220; Mus musculus. [Genome view]
GeneID	104110.
KEGG	mmu:104110.
UCSC	uc007uat.1. mouse.
Organism-specific databases
CTD	104110.
MGI	MGI:99674. Adcy4.
Phylogenomic databases
HOGENOM	HBG445220.
HOVERGEN	Q91WF3.
InParanoid	Q91WF3.
OMA	SCSLFLH.
OrthoDB	EOG9S4S1S.
PhylomeDB	Q91WF3.
Enzyme and pathway databases
BRENDA	4.6.1.1. 244.
Gene expression databases
ArrayExpress	Q91WF3.
Bgee	Q91WF3.
CleanEx	MM_ADCY4.
Genevestigator	Q91WF3.
GermOnline	ENSMUSG00000022220. Mus musculus.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR009398. Aden_cycl-like. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF06327. DUF1053. 1 hit. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 2 hits. [Graphical view]
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 2 hits. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	356540.
SOURCE	Search...

Entry information

Entry name

ADCY4_MOUSE

Accession

Primary (citable) accession number: Q91WF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)