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Q91WD5

- NDUS2_MOUSE

UniProt

Q91WD5 - NDUS2_MOUSE

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

Ndufs2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi326 – 3261Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi332 – 3321Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    5. quinone binding Source: InterPro

    GO - Biological processi

    1. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-49kD
    Short name:
    CI-49kD
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    Gene namesi
    Name:Ndufs2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2385112. Ndufs2.

    Subcellular locationi

    Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: UniProtKB
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionBy similarityAdd
    BLAST
    Chaini34 – 463430NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialPRO_0000019982Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621N6-acetyllysine1 Publication
    Modified residuei118 – 1181Symmetric dimethylarginineBy similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    MaxQBiQ91WD5.
    PaxDbiQ91WD5.
    PRIDEiQ91WD5.

    PTM databases

    PhosphoSiteiQ91WD5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91WD5.
    BgeeiQ91WD5.
    CleanExiMM_NDUFS2.
    GenevestigatoriQ91WD5.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme By similarity. Interacts with NDUFAF3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230541. 1 interaction.
    IntActiQ91WD5. 6 interactions.
    MINTiMINT-1860118.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91WD5.
    SMRiQ91WD5. Positions 80-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 49 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0649.
    GeneTreeiENSGT00390000009529.
    HOGENOMiHOG000228264.
    HOVERGENiHBG000760.
    InParanoidiQ91WD5.
    KOiK03935.
    OMAiQCFALAV.
    OrthoDBiEOG7WT411.
    PhylomeDBiQ91WD5.
    TreeFamiTF300370.

    Family and domain databases

    Gene3Di1.10.645.10. 1 hit.
    HAMAPiMF_01358. NDH1_NuoD.
    InterProiIPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view]
    PfamiPF00346. Complex1_49kDa. 1 hit.
    [Graphical view]
    SUPFAMiSSF56762. SSF56762. 1 hit.
    TIGRFAMsiTIGR01962. NuoD. 1 hit.
    PROSITEiPS00535. COMPLEX1_49K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91WD5-1 [UniParc]FASTAAdd to Basket

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    MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA    50
    VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHPA AHGVLRLVLE 100
    LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA 150
    YSIAVEKLLN IQPPPRAQWI RVLFGEITRI LNHIMAVTTH ALDIGAMTPF 200
    FWMFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL LDDIYEFSKN 250
    FSLRIDEVEE MLTNNRIWRN RTVDIGVVTA EDALNYGFSG VMLRGSGIQW 300
    DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIIEQCLNK 350
    MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI 400
    EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI 450
    IGTQDIVFGE IDR 463
    Length:463
    Mass (Da):52,626
    Last modified:December 1, 2001 - v1
    Checksum:i989934D73F5C8D27
    GO

    Sequence cautioni

    The sequence AAH03898.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 11M → V in AAH03898. (PubMed:15489334)Curated
    Sequence conflicti2 – 21A → R in BAE30656. (PubMed:16141072)Curated
    Sequence conflicti2 – 21A → R in BAE31936. (PubMed:16141072)Curated
    Sequence conflicti81 – 811T → A in BAE27028. (PubMed:16141072)Curated
    Sequence conflicti186 – 1861A → S in BAE27028. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK078474 mRNA. Translation: BAC37293.1.
    AK146269 mRNA. Translation: BAE27028.1.
    AK150431 mRNA. Translation: BAE29554.1.
    AK151746 mRNA. Translation: BAE30656.1.
    AK153364 mRNA. Translation: BAE31936.1.
    AK165426 mRNA. Translation: BAE38181.1.
    BC003898 mRNA. Translation: AAH03898.1. Different initiation.
    BC016097 mRNA. Translation: AAH16097.1.
    CCDSiCCDS15484.1.
    RefSeqiNP_694704.1. NM_153064.4.
    UniGeneiMm.21669.

    Genome annotation databases

    EnsembliENSMUST00000013737; ENSMUSP00000013737; ENSMUSG00000013593.
    GeneIDi226646.
    KEGGimmu:226646.
    UCSCiuc007dnm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK078474 mRNA. Translation: BAC37293.1 .
    AK146269 mRNA. Translation: BAE27028.1 .
    AK150431 mRNA. Translation: BAE29554.1 .
    AK151746 mRNA. Translation: BAE30656.1 .
    AK153364 mRNA. Translation: BAE31936.1 .
    AK165426 mRNA. Translation: BAE38181.1 .
    BC003898 mRNA. Translation: AAH03898.1 . Different initiation.
    BC016097 mRNA. Translation: AAH16097.1 .
    CCDSi CCDS15484.1.
    RefSeqi NP_694704.1. NM_153064.4.
    UniGenei Mm.21669.

    3D structure databases

    ProteinModelPortali Q91WD5.
    SMRi Q91WD5. Positions 80-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230541. 1 interaction.
    IntActi Q91WD5. 6 interactions.
    MINTi MINT-1860118.

    PTM databases

    PhosphoSitei Q91WD5.

    Proteomic databases

    MaxQBi Q91WD5.
    PaxDbi Q91WD5.
    PRIDEi Q91WD5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000013737 ; ENSMUSP00000013737 ; ENSMUSG00000013593 .
    GeneIDi 226646.
    KEGGi mmu:226646.
    UCSCi uc007dnm.1. mouse.

    Organism-specific databases

    CTDi 4720.
    MGIi MGI:2385112. Ndufs2.

    Phylogenomic databases

    eggNOGi COG0649.
    GeneTreei ENSGT00390000009529.
    HOGENOMi HOG000228264.
    HOVERGENi HBG000760.
    InParanoidi Q91WD5.
    KOi K03935.
    OMAi QCFALAV.
    OrthoDBi EOG7WT411.
    PhylomeDBi Q91WD5.
    TreeFami TF300370.

    Miscellaneous databases

    NextBioi 378279.
    PROi Q91WD5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91WD5.
    Bgeei Q91WD5.
    CleanExi MM_NDUFS2.
    Genevestigatori Q91WD5.

    Family and domain databases

    Gene3Di 1.10.645.10. 1 hit.
    HAMAPi MF_01358. NDH1_NuoD.
    InterProi IPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view ]
    Pfami PF00346. Complex1_49kDa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56762. SSF56762. 1 hit.
    TIGRFAMsi TIGR01962. NuoD. 1 hit.
    PROSITEi PS00535. COMPLEX1_49K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BALB/c and C57BL/6J.
      Tissue: Bone marrow.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor and Salivary gland.
    3. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 57-73; 76-108; 128-138; 158-166; 172-179; 210-216; 255-266; 272-323; 373-421; 424-437 AND 441-463, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNDUS2_MOUSE
    AccessioniPrimary (citable) accession number: Q91WD5
    Secondary accession number(s): Q3TNA8
    , Q3U5Y9, Q3UJX7, Q99L23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3