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Q91WD5

- NDUS2_MOUSE

UniProt

Q91WD5 - NDUS2_MOUSE

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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

Ndufs2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi326 – 3261Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi332 – 3321Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  5. quinone binding Source: InterPro

GO - Biological processi

  1. response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene namesi
Name:Ndufs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2385112. Ndufs2.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: UniProtKB
  2. mitochondrion Source: UniProtKB
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionBy similarityAdd
BLAST
Chaini34 – 463430NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialPRO_0000019982Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621N6-acetyllysine1 Publication
Modified residuei118 – 1181Symmetric dimethylarginineBy similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

MaxQBiQ91WD5.
PaxDbiQ91WD5.
PRIDEiQ91WD5.

PTM databases

PhosphoSiteiQ91WD5.

Expressioni

Gene expression databases

BgeeiQ91WD5.
CleanExiMM_NDUFS2.
ExpressionAtlasiQ91WD5. baseline and differential.
GenevestigatoriQ91WD5.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme By similarity. Interacts with NDUFAF3 By similarity.By similarity

Protein-protein interaction databases

BioGridi230541. 1 interaction.
IntActiQ91WD5. 6 interactions.
MINTiMINT-1860118.

Structurei

3D structure databases

ProteinModelPortaliQ91WD5.
SMRiQ91WD5. Positions 80-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0649.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
HOVERGENiHBG000760.
InParanoidiQ91WD5.
KOiK03935.
OMAiQCFALAV.
OrthoDBiEOG7WT411.
PhylomeDBiQ91WD5.
TreeFamiTF300370.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91WD5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA
60 70 80 90 100
VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHPA AHGVLRLVLE
110 120 130 140 150
LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA
160 170 180 190 200
YSIAVEKLLN IQPPPRAQWI RVLFGEITRI LNHIMAVTTH ALDIGAMTPF
210 220 230 240 250
FWMFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL LDDIYEFSKN
260 270 280 290 300
FSLRIDEVEE MLTNNRIWRN RTVDIGVVTA EDALNYGFSG VMLRGSGIQW
310 320 330 340 350
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIIEQCLNK
360 370 380 390 400
MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI
410 420 430 440 450
EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI
460
IGTQDIVFGE IDR
Length:463
Mass (Da):52,626
Last modified:December 1, 2001 - v1
Checksum:i989934D73F5C8D27
GO

Sequence cautioni

The sequence AAH03898.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → V in AAH03898. (PubMed:15489334)Curated
Sequence conflicti2 – 21A → R in BAE30656. (PubMed:16141072)Curated
Sequence conflicti2 – 21A → R in BAE31936. (PubMed:16141072)Curated
Sequence conflicti81 – 811T → A in BAE27028. (PubMed:16141072)Curated
Sequence conflicti186 – 1861A → S in BAE27028. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK078474 mRNA. Translation: BAC37293.1.
AK146269 mRNA. Translation: BAE27028.1.
AK150431 mRNA. Translation: BAE29554.1.
AK151746 mRNA. Translation: BAE30656.1.
AK153364 mRNA. Translation: BAE31936.1.
AK165426 mRNA. Translation: BAE38181.1.
BC003898 mRNA. Translation: AAH03898.1. Different initiation.
BC016097 mRNA. Translation: AAH16097.1.
CCDSiCCDS15484.1.
RefSeqiNP_694704.1. NM_153064.4.
UniGeneiMm.21669.

Genome annotation databases

EnsembliENSMUST00000013737; ENSMUSP00000013737; ENSMUSG00000013593.
GeneIDi226646.
KEGGimmu:226646.
UCSCiuc007dnm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK078474 mRNA. Translation: BAC37293.1 .
AK146269 mRNA. Translation: BAE27028.1 .
AK150431 mRNA. Translation: BAE29554.1 .
AK151746 mRNA. Translation: BAE30656.1 .
AK153364 mRNA. Translation: BAE31936.1 .
AK165426 mRNA. Translation: BAE38181.1 .
BC003898 mRNA. Translation: AAH03898.1 . Different initiation.
BC016097 mRNA. Translation: AAH16097.1 .
CCDSi CCDS15484.1.
RefSeqi NP_694704.1. NM_153064.4.
UniGenei Mm.21669.

3D structure databases

ProteinModelPortali Q91WD5.
SMRi Q91WD5. Positions 80-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230541. 1 interaction.
IntActi Q91WD5. 6 interactions.
MINTi MINT-1860118.

PTM databases

PhosphoSitei Q91WD5.

Proteomic databases

MaxQBi Q91WD5.
PaxDbi Q91WD5.
PRIDEi Q91WD5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000013737 ; ENSMUSP00000013737 ; ENSMUSG00000013593 .
GeneIDi 226646.
KEGGi mmu:226646.
UCSCi uc007dnm.1. mouse.

Organism-specific databases

CTDi 4720.
MGIi MGI:2385112. Ndufs2.

Phylogenomic databases

eggNOGi COG0649.
GeneTreei ENSGT00390000009529.
HOGENOMi HOG000228264.
HOVERGENi HBG000760.
InParanoidi Q91WD5.
KOi K03935.
OMAi QCFALAV.
OrthoDBi EOG7WT411.
PhylomeDBi Q91WD5.
TreeFami TF300370.

Miscellaneous databases

NextBioi 378279.
PROi Q91WD5.
SOURCEi Search...

Gene expression databases

Bgeei Q91WD5.
CleanExi MM_NDUFS2.
ExpressionAtlasi Q91WD5. baseline and differential.
Genevestigatori Q91WD5.

Family and domain databases

Gene3Di 1.10.645.10. 1 hit.
HAMAPi MF_01358. NDH1_NuoD.
InterProi IPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view ]
Pfami PF00346. Complex1_49kDa. 1 hit.
[Graphical view ]
SUPFAMi SSF56762. SSF56762. 1 hit.
TIGRFAMsi TIGR01962. NuoD. 1 hit.
PROSITEi PS00535. COMPLEX1_49K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BALB/c and C57BL/6J.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor and Salivary gland.
  3. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 57-73; 76-108; 128-138; 158-166; 172-179; 210-216; 255-266; 272-323; 373-421; 424-437 AND 441-463, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNDUS2_MOUSE
AccessioniPrimary (citable) accession number: Q91WD5
Secondary accession number(s): Q3TNA8
, Q3U5Y9, Q3UJX7, Q99L23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3