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Protein

Transient receptor potential cation channel subfamily V member 6

Gene

Trpv6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium selective cation channel probably involved in Ca2+ uptake in various tissues, including Ca2+ reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.2 Publications

GO - Molecular functioni

  • calcium channel activity Source: MGI
  • calmodulin binding Source: MGI

GO - Biological processi

  • calcium ion homeostasis Source: MGI
  • calcium ion transport Source: MGI
  • parathyroid hormone secretion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 6
Short name:
TrpV6
Alternative name(s):
Calcium transport protein 1
Short name:
CaT1
Epithelial calcium channel 2
Short name:
ECaC2
Gene namesi
Name:Trpv6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1927259. Trpv6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 367367CytoplasmicSequence analysisAdd
BLAST
Transmembranei368 – 38821HelicalSequence analysisAdd
BLAST
Topological domaini389 – 42638ExtracellularSequence analysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST
Topological domaini448 – 45811CytoplasmicSequence analysisAdd
BLAST
Transmembranei459 – 47921HelicalSequence analysisAdd
BLAST
Topological domaini480 – 4878ExtracellularSequence analysis
Transmembranei488 – 50821HelicalSequence analysisAdd
BLAST
Topological domaini509 – 53123CytoplasmicSequence analysisAdd
BLAST
Transmembranei532 – 55221HelicalSequence analysisAdd
BLAST
Intramembranei563 – 58523Pore-formingAdd
BLAST
Transmembranei598 – 61821HelicalSequence analysisAdd
BLAST
Topological domaini619 – 767149CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi577 – 5771L → C: Abolishes channel activity. 1 Publication
Mutagenesisi581 – 5811D → A: Abolishes binding of Mg(2+) and increases pore diameter. 2 Publications
Mutagenesisi581 – 5811D → C: Abolishes channel activity. 2 Publications
Mutagenesisi581 – 5811D → G: Increases pore diameter. 2 Publications
Mutagenesisi586 – 5861Y → C: Abolishes channel activity. 1 Publication
Mutagenesisi639 – 6391T → A: Abolishes plasma membrane localization and channel activity. 1 Publication

Chemistry

GuidetoPHARMACOLOGYi512.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Transient receptor potential cation channel subfamily V member 6PRO_0000215355Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011Phosphotyrosine; by SRCBy similarity
Modified residuei202 – 2021Phosphotyrosine; by SRCBy similarity
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.2 Publications
Phosphorylation at Tyr-201 and Tyr-202 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at these sites by PTPN1 (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ91WD2.
PRIDEiQ91WD2.

PTM databases

iPTMnetiQ91WD2.
PhosphoSiteiQ91WD2.

Expressioni

Tissue specificityi

Abundantly expressed in pancreas and placenta, and to a much lesser extend in stomach and kidney. Not detected in intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000029868.

Interactioni

Subunit structurei

Interacts with TCAF1 and TCAF2 (By similarity). Homotetramer and probably heterotetramer with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031902.

Structurei

Secondary structure

1
767
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi88 – 947Combined sources
Helixi98 – 1058Combined sources
Helixi122 – 1287Combined sources
Helixi132 – 14110Combined sources
Helixi143 – 1475Combined sources
Turni153 – 1575Combined sources
Helixi160 – 1667Combined sources
Helixi170 – 1789Combined sources
Helixi190 – 1923Combined sources
Helixi206 – 2138Combined sources
Helixi216 – 2249Combined sources
Helixi239 – 2446Combined sources
Helixi249 – 26113Combined sources
Helixi271 – 2733Combined sources
Helixi282 – 2898Combined sources
Helixi292 – 30413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RFAX-ray1.70A84-305[»]
ProteinModelPortaliQ91WD2.
SMRiQ91WD2. Positions 84-651.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91WD2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati84 – 11431ANK 1PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati118 – 14730ANK 2PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati156 – 18530ANK 3PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati202 – 23130ANK 4PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati235 – 27642ANK 5PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati278 – 30730ANK 6PROSITE-ProRule annotation1 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 14311Interaction with calmodulinAdd
BLAST
Regioni637 – 6415Interaction with S100A10By similarity
Regioni689 – 70719Interaction with calmodulinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi580 – 5845Selectivity filter

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ91WD2.
KOiK04975.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91WD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLQREDRP ALGGANVAPG SSPVGVWHQP QPPKEPAFHP MGWSLPKEKG
60 70 80 90 100
LILCLWNKFC RWFHRQESWA QSRDEQNLLQ QKRIWESPLL LAAKENDVQA
110 120 130 140 150
LSKLLKFEGC EVHQRGAMGE TALHIAALYD NLEAAMVLME AAPELVFEPM
160 170 180 190 200
TSELYEGQTA LHIAVINQNV NLVRALLARG ASVSARATGS VFHYRPHNLI
210 220 230 240 250
YYGEHPLSFA ACVGSEEIVR LLIEHGADIR AQDSLGNTVL HILILQPNKT
260 270 280 290 300
FACQMYNLLL SYDGGDHLKS LELVPNNQGL TPFKLAGVEG NIVMFQHLMQ
310 320 330 340 350
KRKHIQWTYG PLTSTLYDLT EIDSSGDDQS LLELIVTTKK REARQILDQT
360 370 380 390 400
PVKELVSLKW KRYGRPYFCV LGAIYVLYII CFTMCCVYRP LKPRITNRTN
410 420 430 440 450
PRDNTLMQQK LLQEAYVTPK DDLRLVGELV SIVGAVIILL VEIPDIFRLG
460 470 480 490 500
VTRFFGQTIL GGPFHVIIIT YAFMVLVTMV MRLTNVDGEV VPMSFALVLG
510 520 530 540 550
WCNVMYFARG FQMLGPFTIM IQKMIFGDLM RFCWLMAVVI LGFASAFYII
560 570 580 590 600
FQTEDPDELG HFYDYPMALF STFELFLTII DGPANYDVDL PFMYSVTYAA
610 620 630 640 650
FAIIATLLML NLLIAMMGDT HWRVAHERDE LWRAQVVATT VMLERKLPRC
660 670 680 690 700
LWPRSGICGR EYGLGDRWFL RVEDRQDLNR QRIRRYAQAF QQQDGLYSED
710 720 730 740 750
LEKDSGEKLE TARPFGAYLS FPTPSVSRST SRSSTNWERL RQGALRKDLR
760
GIINRGLEDG EGWEYQI
Length:767
Mass (Da):87,387
Last modified:April 1, 2015 - v2
Checksum:iFBD937FB9C55F9BA
GO

Sequence cautioni

The sequence AAH16101 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical ACG threonine codon.1 Publication
The sequence BAC36699 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical ACG threonine codon.1 Publication

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → E in BAC36699 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077234 mRNA. Translation: BAC36699.1. Sequence problems.
BC016101 mRNA. Translation: AAH16101.1. Sequence problems.
AJ542487 mRNA. Translation: CAD62684.1.
CCDSiCCDS20052.1.
PIRiJC7796.
RefSeqiNP_071858.3. NM_022413.4.
UniGeneiMm.296889.

Genome annotation databases

GeneIDi64177.
KEGGimmu:64177.
UCSCiuc009bqc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK077234 mRNA. Translation: BAC36699.1. Sequence problems.
BC016101 mRNA. Translation: AAH16101.1. Sequence problems.
AJ542487 mRNA. Translation: CAD62684.1.
CCDSiCCDS20052.1.
PIRiJC7796.
RefSeqiNP_071858.3. NM_022413.4.
UniGeneiMm.296889.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RFAX-ray1.70A84-305[»]
ProteinModelPortaliQ91WD2.
SMRiQ91WD2. Positions 84-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031902.

Chemistry

GuidetoPHARMACOLOGYi512.

PTM databases

iPTMnetiQ91WD2.
PhosphoSiteiQ91WD2.

Proteomic databases

PaxDbiQ91WD2.
PRIDEiQ91WD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64177.
KEGGimmu:64177.
UCSCiuc009bqc.2. mouse.

Organism-specific databases

CTDi55503.
MGIiMGI:1927259. Trpv6.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ91WD2.
KOiK04975.
TreeFamiTF314711.

Miscellaneous databases

EvolutionaryTraceiQ91WD2.
PROiQ91WD2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029868.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV6_MOUSE
AccessioniPrimary (citable) accession number: Q91WD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 1, 2015
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.