Reviewed, UniProtKB/Swiss-Prot Q91WC3 (ACSL6_MOUSE)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase 6
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase 6
      Short name=LACS 6

Gene names

Name:	Acsl6
Synonyms:	Facl6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome Mitochondrion Mitochondrion outer membrane Peroxisome
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neuroblast proliferation Ref.1 Inferred from direct assay. Source: MGI
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW peroxisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Ref.1 Inferred from direct assay. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91WC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91WC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLTFFLVSGGSLWLFAEIALSLLEKM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Long-chain-fatty-acid--CoA ligase 6		PRO_0000193116
Regions
	Transmembrane	25 – 45	21	Signal-anchor for type III membrane protein Potential
	Topological domain	46 – 697	652	Cytoplasmic Potential
Natural variations
	Alternative sequence	1	1	M → MLTFFLVSGGSLWLFAEIAL SLLEKM in isoform 2.		VSP_037824
Experimental info
	Sequence conflict	364	1	P → S in AAO38689. Ref.1
	Sequence conflict	680	1	E → G in AAO38689. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: ADAFC99E5D97DC22	FASTA	697	78,017
	10 20 30 40 50 60 MQTQEILRIL RLPELSDLGQ FFRSLSATTL VSVGALAAVL AYWLTHRPKA LQPPCNLLKQ 70 80 90 100 110 120 SEEVEDGGGA RRSVIGGCTQ LLTHYYDDAR TMYQVFRRGL SISGNGPCLG FRKPEQPYQW 130 140 150 160 170 180 LSYQEVAKRA EFLGSGLLQH DCKVGTEQFV GVFAQNRPEW IIAELACYTY SMVVVPLYDT 190 200 210 220 230 240 LGPGSISYII NTADICTVIV DKPHKATLLL EHVERKETPG LKLVILMEPF EDALRERGKK 250 260 270 280 290 300 CGVDIKSMQA IEDCGRENHH APVPPRPDDL SIVCFTSGTT GNPKGAMLTH GNVVADFSGF 310 320 330 340 350 360 LKVTEKVIFP RQDDVLISFL PLAHMFERVI QSVVYCHGGR VGFFQGDIRL LSDDMKALRP 370 380 390 400 410 420 TIFPVVPRLL NRMYDKIFHQ ADTSLKRWLL EFAAKRKQAE VRSGIIRNNS IWDELFFNKI 430 440 450 460 470 480 QASLGGHVRM IVTGAAPASP TVLGFLRAAL GCQVYEGYGQ TECTAGCTFT TPGDWTSGHV 490 500 510 520 530 540 GAPLPCNHIK LVDAEELNYW TCKGEGEICV KGPNVFKGYL KDEDRTKEAL DSDGWLHTGD 550 560 570 580 590 600 IGKWLPEGTL KIIDRKKHIF KLAQGEYVAP EKIENIYIRS EPVAQIYVHG DSLKAFLVGI 610 620 630 640 650 660 VVPDPEVMPS WAQKKGIEGT YQELCMKKEL KKAILDDMVM LGKESGLHSF EQVKAIYIHC 670 680 690 DMFSVQNGLL TPTLKAKRPE LREYFKKQIE ELYLVSV
	Isoform 2. Checksum: 989C3ADB76346764	FASTA	722	80,786
	10 20 30 40 50 60 MLTFFLVSGG SLWLFAEIAL SLLEKMQTQE ILRILRLPEL SDLGQFFRSL SATTLVSVGA 70 80 90 100 110 120 LAAVLAYWLT HRPKALQPPC NLLKQSEEVE DGGGARRSVI GGCTQLLTHY YDDARTMYQV 130 140 150 160 170 180 FRRGLSISGN GPCLGFRKPE QPYQWLSYQE VAKRAEFLGS GLLQHDCKVG TEQFVGVFAQ 190 200 210 220 230 240 NRPEWIIAEL ACYTYSMVVV PLYDTLGPGS ISYIINTADI CTVIVDKPHK ATLLLEHVER 250 260 270 280 290 300 KETPGLKLVI LMEPFEDALR ERGKKCGVDI KSMQAIEDCG RENHHAPVPP RPDDLSIVCF 310 320 330 340 350 360 TSGTTGNPKG AMLTHGNVVA DFSGFLKVTE KVIFPRQDDV LISFLPLAHM FERVIQSVVY 370 380 390 400 410 420 CHGGRVGFFQ GDIRLLSDDM KALRPTIFPV VPRLLNRMYD KIFHQADTSL KRWLLEFAAK 430 440 450 460 470 480 RKQAEVRSGI IRNNSIWDEL FFNKIQASLG GHVRMIVTGA APASPTVLGF LRAALGCQVY 490 500 510 520 530 540 EGYGQTECTA GCTFTTPGDW TSGHVGAPLP CNHIKLVDAE ELNYWTCKGE GEICVKGPNV 550 560 570 580 590 600 FKGYLKDEDR TKEALDSDGW LHTGDIGKWL PEGTLKIIDR KKHIFKLAQG EYVAPEKIEN 610 620 630 640 650 660 IYIRSEPVAQ IYVHGDSLKA FLVGIVVPDP EVMPSWAQKK GIEGTYQELC MKKELKKAIL 670 680 690 700 710 720 DDMVMLGKES GLHSFEQVKA IYIHCDMFSV QNGLLTPTLK AKRPELREYF KKQIEELYLV SV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel murine long-chain acyl-CoA synthetase expressed in brain participates in neuronal cell proliferation." Kee H.J., Koh J.T., Yang S.Y., Lee Z.H., Baik Y.H., Kim K.K. Biochem. Biophys. Res. Commun. 305:925-933(2003) [PubMed: 12767919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: ICR. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY167035 mRNA. Translation: AAO38689.1. BC016114 mRNA. Translation: AAH16114.1.
IPI	IPI00123390. IPI00625955.
RefSeq	NP_001028770.1.
UniGene	Mm.267478
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91WC3.
Proteomic databases
PRIDE	Q91WC3.
Genome annotation databases
Ensembl	ENSMUST00000072178; ENSMUSP00000072040; ENSMUSG00000020333; Mus musculus. [Genome view] ENSMUST00000093106; ENSMUSP00000090795; ENSMUSG00000020333; Mus musculus. [Genome view] ENSMUST00000094194; ENSMUSP00000091746; ENSMUSG00000020333; Mus musculus. [Genome view] ENSMUST00000108904; ENSMUSP00000104532; ENSMUSG00000020333; Mus musculus. [Genome view] ENSMUST00000108905; ENSMUSP00000104533; ENSMUSG00000020333; Mus musculus. [Genome view]
GeneID	216739.
UCSC	uc007ixo.1. mouse.
Organism-specific databases
CTD	216739.
MGI	MGI:894291. Acsl6.
Phylogenomic databases
HOVERGEN	Q91WC3.
Enzyme and pathway databases
BRENDA	6.2.1.3. 244.
Gene expression databases
ArrayExpress	Q91WC3.
Bgee	Q91WC3.
CleanEx	MM_ACSL6.
Genevestigator	Q91WC3.
GermOnline	ENSMUSG00000020333. Mus musculus.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 2 hits. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	375272.
SOURCE	Search...

Entry information

Entry name

ACSL6_MOUSE

Accession

Primary (citable) accession number: Q91WC3
Secondary accession number(s): Q80ZF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)