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Q91WC3 (ACSL6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase 6

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase 6
Short name=LACS 6
Gene names
Name:Acsl6
Synonyms:Facl6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length697 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid By similarity.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactor

Magnesium By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
Mitochondrion
Mitochondrion outer membrane
Peroxisome
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to insulin stimulus

Inferred from electronic annotation. Source: Ensembl

fatty acid transport

Inferred from electronic annotation. Source: Ensembl

neuroblast proliferation

Inferred from direct assay Ref.1. Source: MGI

neuron development

Inferred from electronic annotation. Source: Ensembl

phospholipid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of plasma membrane long-chain fatty acid transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of triglyceride biosynthetic process

Inferred from electronic annotation. Source: Ensembl

response to gravity

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

response to steroid hormone

Inferred from electronic annotation. Source: Ensembl

triglyceride metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrial outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853. Source: MGI

nucleus

Inferred from electronic annotation. Source: Ensembl

peroxisomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

long-chain fatty acid-CoA ligase activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q91WC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q91WC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLTFFLVSGGSLWLFAEIALSLLEKM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 697697Long-chain-fatty-acid--CoA ligase 6
PRO_0000193116

Regions

Transmembrane25 – 4521Helical; Signal-anchor for type III membrane protein; Potential
Topological domain46 – 697652Cytoplasmic Potential

Natural variations

Alternative sequence11M → MLTFFLVSGGSLWLFAEIAL SLLEKM in isoform 2.
VSP_037824

Experimental info

Sequence conflict3641P → S in AAO38689. Ref.1
Sequence conflict6801E → G in AAO38689. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: ADAFC99E5D97DC22

FASTA69778,017
        10         20         30         40         50         60 
MQTQEILRIL RLPELSDLGQ FFRSLSATTL VSVGALAAVL AYWLTHRPKA LQPPCNLLKQ 

        70         80         90        100        110        120 
SEEVEDGGGA RRSVIGGCTQ LLTHYYDDAR TMYQVFRRGL SISGNGPCLG FRKPEQPYQW 

       130        140        150        160        170        180 
LSYQEVAKRA EFLGSGLLQH DCKVGTEQFV GVFAQNRPEW IIAELACYTY SMVVVPLYDT 

       190        200        210        220        230        240 
LGPGSISYII NTADICTVIV DKPHKATLLL EHVERKETPG LKLVILMEPF EDALRERGKK 

       250        260        270        280        290        300 
CGVDIKSMQA IEDCGRENHH APVPPRPDDL SIVCFTSGTT GNPKGAMLTH GNVVADFSGF 

       310        320        330        340        350        360 
LKVTEKVIFP RQDDVLISFL PLAHMFERVI QSVVYCHGGR VGFFQGDIRL LSDDMKALRP 

       370        380        390        400        410        420 
TIFPVVPRLL NRMYDKIFHQ ADTSLKRWLL EFAAKRKQAE VRSGIIRNNS IWDELFFNKI 

       430        440        450        460        470        480 
QASLGGHVRM IVTGAAPASP TVLGFLRAAL GCQVYEGYGQ TECTAGCTFT TPGDWTSGHV 

       490        500        510        520        530        540 
GAPLPCNHIK LVDAEELNYW TCKGEGEICV KGPNVFKGYL KDEDRTKEAL DSDGWLHTGD 

       550        560        570        580        590        600 
IGKWLPEGTL KIIDRKKHIF KLAQGEYVAP EKIENIYIRS EPVAQIYVHG DSLKAFLVGI 

       610        620        630        640        650        660 
VVPDPEVMPS WAQKKGIEGT YQELCMKKEL KKAILDDMVM LGKESGLHSF EQVKAIYIHC 

       670        680        690 
DMFSVQNGLL TPTLKAKRPE LREYFKKQIE ELYLVSV 

« Hide

Isoform 2 [UniParc].

Checksum: 989C3ADB76346764
Show »

FASTA72280,786

References

« Hide 'large scale' references
[1]"A novel murine long-chain acyl-CoA synthetase expressed in brain participates in neuronal cell proliferation."
Kee H.J., Koh J.T., Yang S.Y., Lee Z.H., Baik Y.H., Kim K.K.
Biochem. Biophys. Res. Commun. 305:925-933(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: ICR.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY167035 mRNA. Translation: AAO38689.1.
BC016114 mRNA. Translation: AAH16114.1.
RefSeqNP_001028770.1. NM_001033598.1.
XP_006532906.1. XM_006532843.1.
XP_006532907.1. XM_006532844.1.
UniGeneMm.267478.

3D structure databases

ProteinModelPortalQ91WC3.
SMRQ91WC3. Positions 92-690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229776. 1 interaction.
IntActQ91WC3. 3 interactions.
MINTMINT-1861033.
STRING10090.ENSMUSP00000104533.

PTM databases

PhosphoSiteQ91WC3.

Proteomic databases

PaxDbQ91WC3.
PRIDEQ91WC3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072178; ENSMUSP00000072040; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000093106; ENSMUSP00000090795; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000094194; ENSMUSP00000091746; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000108905; ENSMUSP00000104533; ENSMUSG00000020333. [Q91WC3-2]
GeneID216739.
KEGGmmu:216739.
UCSCuc007ixo.1. mouse. [Q91WC3-1]

Organism-specific databases

CTD23305.
MGIMGI:894291. Acsl6.

Phylogenomic databases

eggNOGCOG1022.
GeneTreeENSGT00690000101725.
HOGENOMHOG000159459.
HOVERGENHBG050452.
KOK01897.
OrthoDBEOG71CFKN.
PhylomeDBQ91WC3.

Enzyme and pathway databases

BRENDA6.2.1.3. 3474.

Gene expression databases

ArrayExpressQ91WC3.
BgeeQ91WC3.
CleanExMM_ACSL6.
GenevestigatorQ91WC3.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375272.
PROQ91WC3.
SOURCESearch...

Entry information

Entry nameACSL6_MOUSE
AccessionPrimary (citable) accession number: Q91WC3
Secondary accession number(s): Q80ZF1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot