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Protein

Long-chain-fatty-acid--CoA ligase 6

Gene

Acsl6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid (By similarity).By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. enzyme binding Source: MGI
  3. long-chain fatty acid-CoA ligase activity Source: MGI
  4. protein homodimerization activity Source: MGI

GO - Biological processi

  1. cellular response to insulin stimulus Source: Ensembl
  2. fatty acid transport Source: Ensembl
  3. long-chain fatty acid metabolic process Source: MGI
  4. neuroblast proliferation Source: MGI
  5. neuron development Source: Ensembl
  6. phospholipid biosynthetic process Source: Ensembl
  7. positive regulation of neuron projection development Source: Ensembl
  8. positive regulation of plasma membrane long-chain fatty acid transport Source: Ensembl
  9. positive regulation of triglyceride biosynthetic process Source: Ensembl
  10. response to gravity Source: Ensembl
  11. response to hypoxia Source: Ensembl
  12. response to nutrient Source: Ensembl
  13. response to steroid hormone Source: Ensembl
  14. triglyceride metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiREACT_292148. Synthesis of very long-chain fatty acyl-CoAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 6 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 6
Short name:
LACS 6
Gene namesi
Name:Acsl6
Synonyms:Facl6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:894291. Acsl6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
BLAST
Topological domaini46 – 697652CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
  4. mitochondrial outer membrane Source: UniProtKB-SubCell
  5. mitochondrion Source: MGI
  6. nucleus Source: Ensembl
  7. peroxisomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697Long-chain-fatty-acid--CoA ligase 6PRO_0000193116Add
BLAST

Proteomic databases

MaxQBiQ91WC3.
PaxDbiQ91WC3.
PRIDEiQ91WC3.

PTM databases

PhosphoSiteiQ91WC3.

Expressioni

Gene expression databases

BgeeiQ91WC3.
CleanExiMM_ACSL6.
ExpressionAtlasiQ91WC3. baseline and differential.
GenevestigatoriQ91WC3.

Interactioni

Protein-protein interaction databases

BioGridi229776. 1 interaction.
IntActiQ91WC3. 3 interactions.
MINTiMINT-1861033.
STRINGi10090.ENSMUSP00000104533.

Structurei

3D structure databases

ProteinModelPortaliQ91WC3.
SMRiQ91WC3. Positions 114-634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000159459.
HOVERGENiHBG050452.
InParanoidiQ91WC3.
KOiK01897.
OrthoDBiEOG71CFKN.
PhylomeDBiQ91WC3.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTQEILRIL RLPELSDLGQ FFRSLSATTL VSVGALAAVL AYWLTHRPKA
60 70 80 90 100
LQPPCNLLKQ SEEVEDGGGA RRSVIGGCTQ LLTHYYDDAR TMYQVFRRGL
110 120 130 140 150
SISGNGPCLG FRKPEQPYQW LSYQEVAKRA EFLGSGLLQH DCKVGTEQFV
160 170 180 190 200
GVFAQNRPEW IIAELACYTY SMVVVPLYDT LGPGSISYII NTADICTVIV
210 220 230 240 250
DKPHKATLLL EHVERKETPG LKLVILMEPF EDALRERGKK CGVDIKSMQA
260 270 280 290 300
IEDCGRENHH APVPPRPDDL SIVCFTSGTT GNPKGAMLTH GNVVADFSGF
310 320 330 340 350
LKVTEKVIFP RQDDVLISFL PLAHMFERVI QSVVYCHGGR VGFFQGDIRL
360 370 380 390 400
LSDDMKALRP TIFPVVPRLL NRMYDKIFHQ ADTSLKRWLL EFAAKRKQAE
410 420 430 440 450
VRSGIIRNNS IWDELFFNKI QASLGGHVRM IVTGAAPASP TVLGFLRAAL
460 470 480 490 500
GCQVYEGYGQ TECTAGCTFT TPGDWTSGHV GAPLPCNHIK LVDAEELNYW
510 520 530 540 550
TCKGEGEICV KGPNVFKGYL KDEDRTKEAL DSDGWLHTGD IGKWLPEGTL
560 570 580 590 600
KIIDRKKHIF KLAQGEYVAP EKIENIYIRS EPVAQIYVHG DSLKAFLVGI
610 620 630 640 650
VVPDPEVMPS WAQKKGIEGT YQELCMKKEL KKAILDDMVM LGKESGLHSF
660 670 680 690
EQVKAIYIHC DMFSVQNGLL TPTLKAKRPE LREYFKKQIE ELYLVSV
Length:697
Mass (Da):78,017
Last modified:December 1, 2001 - v1
Checksum:iADAFC99E5D97DC22
GO
Isoform 2 (identifier: Q91WC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLTFFLVSGGSLWLFAEIALSLLEKM

Show »
Length:722
Mass (Da):80,786
Checksum:i989C3ADB76346764
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti364 – 3641P → S in AAO38689 (PubMed:12767919).Curated
Sequence conflicti680 – 6801E → G in AAO38689 (PubMed:12767919).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MLTFFLVSGGSLWLFAEIAL SLLEKM in isoform 2. 1 PublicationVSP_037824

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY167035 mRNA. Translation: AAO38689.1.
BC016114 mRNA. Translation: AAH16114.1.
CCDSiCCDS24695.1. [Q91WC3-2]
CCDS24696.1. [Q91WC3-1]
RefSeqiNP_001028770.1. NM_001033598.1. [Q91WC3-1]
XP_006532906.1. XM_006532843.1. [Q91WC3-1]
XP_006532907.1. XM_006532844.1. [Q91WC3-1]
UniGeneiMm.267478.

Genome annotation databases

EnsembliENSMUST00000072178; ENSMUSP00000072040; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000093106; ENSMUSP00000090795; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000094194; ENSMUSP00000091746; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000108905; ENSMUSP00000104533; ENSMUSG00000020333. [Q91WC3-2]
GeneIDi216739.
KEGGimmu:216739.
UCSCiuc007ixo.1. mouse. [Q91WC3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY167035 mRNA. Translation: AAO38689.1.
BC016114 mRNA. Translation: AAH16114.1.
CCDSiCCDS24695.1. [Q91WC3-2]
CCDS24696.1. [Q91WC3-1]
RefSeqiNP_001028770.1. NM_001033598.1. [Q91WC3-1]
XP_006532906.1. XM_006532843.1. [Q91WC3-1]
XP_006532907.1. XM_006532844.1. [Q91WC3-1]
UniGeneiMm.267478.

3D structure databases

ProteinModelPortaliQ91WC3.
SMRiQ91WC3. Positions 114-634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229776. 1 interaction.
IntActiQ91WC3. 3 interactions.
MINTiMINT-1861033.
STRINGi10090.ENSMUSP00000104533.

PTM databases

PhosphoSiteiQ91WC3.

Proteomic databases

MaxQBiQ91WC3.
PaxDbiQ91WC3.
PRIDEiQ91WC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072178; ENSMUSP00000072040; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000093106; ENSMUSP00000090795; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000094194; ENSMUSP00000091746; ENSMUSG00000020333. [Q91WC3-1]
ENSMUST00000108905; ENSMUSP00000104533; ENSMUSG00000020333. [Q91WC3-2]
GeneIDi216739.
KEGGimmu:216739.
UCSCiuc007ixo.1. mouse. [Q91WC3-1]

Organism-specific databases

CTDi23305.
MGIiMGI:894291. Acsl6.

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000159459.
HOVERGENiHBG050452.
InParanoidiQ91WC3.
KOiK01897.
OrthoDBiEOG71CFKN.
PhylomeDBiQ91WC3.

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiREACT_292148. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

NextBioi375272.
PROiQ91WC3.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WC3.
CleanExiMM_ACSL6.
ExpressionAtlasiQ91WC3. baseline and differential.
GenevestigatoriQ91WC3.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel murine long-chain acyl-CoA synthetase expressed in brain participates in neuronal cell proliferation."
    Kee H.J., Koh J.T., Yang S.Y., Lee Z.H., Baik Y.H., Kim K.K.
    Biochem. Biophys. Res. Commun. 305:925-933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Retina.

Entry informationi

Entry nameiACSL6_MOUSE
AccessioniPrimary (citable) accession number: Q91WC3
Secondary accession number(s): Q80ZF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.