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Protein

Histone-lysine N-methyltransferase setd3

Gene

Setd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3 (H3K4me and H3K36me) (PubMed:21832073). Acts as a transcriptional activator (PubMed:21832073). Plays an important role in the transcriptional regulation of muscle cell differentiation via interaction with MYOD1 (PubMed:21832073).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase setd3 (EC:2.1.1.431 Publication)
Alternative name(s):
Endothelial differentiation inhibitory protein D10
SET domain-containing protein 3
Gene namesi
Name:Setd3
Synonyms:D12Ertd771e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1289184. Setd3.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002541761 – 594Histone-lysine N-methyltransferase setd3Add BLAST594

Proteomic databases

EPDiQ91WC0.
PaxDbiQ91WC0.
PeptideAtlasiQ91WC0.
PRIDEiQ91WC0.

PTM databases

iPTMnetiQ91WC0.
PhosphoSitePlusiQ91WC0.

Expressioni

Tissue specificityi

Prominently expressed in the heart and skeletal muscles and is also detected weakly in the stomach, small intestine, and colon.1 Publication

Gene expression databases

BgeeiENSMUSG00000056770.
CleanExiMM_SETD3.
ExpressionAtlasiQ91WC0. baseline and differential.
GenevisibleiQ91WC0. MM.

Interactioni

Subunit structurei

Interacts with MYOD1.1 Publication

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi206736. 6 interactors.
IntActiQ91WC0. 6 interactors.
STRINGi10090.ENSMUSP00000066413.

Structurei

3D structure databases

ProteinModelPortaliQ91WC0.
SMRiQ91WC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 314SETPROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1337. Eukaryota.
ENOG410Y7DR. LUCA.
GeneTreeiENSGT00850000132334.
HOGENOMiHOG000049107.
HOVERGENiHBG062823.
InParanoidiQ91WC0.
KOiK19199.
OMAiNGTRSEN.
OrthoDBiEOG091G087D.
PhylomeDBiQ91WC0.
TreeFamiTF354226.

Family and domain databases

Gene3Di3.90.1420.10. 1 hit.
InterProiIPR025785. Hist-Lys_N-MeTrfase_SETD3.
IPR015353. Rubisco_LSMT_subst-bd.
IPR001214. SET_dom.
[Graphical view]
PfamiPF09273. Rubis-subs-bind. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SUPFAMiSSF81822. SSF81822. 1 hit.
PROSITEiPS51565. SAM_MT43_SETD3. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKSRVKTQ KSGTGATATV SPKEILNLTS ELLQKCSSPA PSPGKEWEEY
60 70 80 90 100
TQIRALVEKI RKKQKGLSVT FDGKREDYFP DLMKWASENG ASVEGFEMVN
110 120 130 140 150
FKEEGFGLRA TRDIKAEELF LWVPRKLLMT VESAKNSVLG PLYSQDRILQ
160 170 180 190 200
AMGNIALAFH LLCERASPNS FWQPYIQTLP SEYDTPLYFE EEEVRCLQST
210 220 230 240 250
QAIHDVFSQY KNTARQYAYF YKVIQTHPHA NKLPLKESFT YEDYRWAVSS
260 270 280 290 300
VMTRQNQIPT EDGSRVTLAL IPLWDMCNHT NGLITTGYNL EDDRCECVAL
310 320 330 340 350
QDFQAGDQIY IFYGTRSNAE FVIHSGFFFD NNSHDRVKIK LGVSKSDRLY
360 370 380 390 400
AMKAEVLARA GIPTSSVFAL HSTEPPISAQ LLAFLRVFCM TEEELKEHLL
410 420 430 440 450
GDSAIDRIFT LGNAEFPVSW DNEVKLWTFL EDRASLLLKT YKTTIEEDKI
460 470 480 490 500
VLKNPDLSVR ATMAIKLRLG EKEILEKAVK SAAVNREYYR KHMEERAPLP
510 520 530 540 550
RYEESDLGLL EGGVGDSRLP LVLRKLEEEA GVQESLSLTE TVSKVKAAEN
560 570 580 590
GLVNGENLIP NGTRSENESL SPEESENVTG EESSGSMAKV KERL
Length:594
Mass (Da):67,176
Last modified:December 1, 2001 - v1
Checksum:i67DA47889A5C9F55
GO
Isoform 2 (identifier: Q91WC0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     226-283: Missing.

Show »
Length:408
Mass (Da):46,072
Checksum:i051A8DA6EA0643BA
GO
Isoform 3 (identifier: Q91WC0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-351: Missing.

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,001
Checksum:iC3FD156DC6D07D43
GO
Isoform 4 (identifier: Q91WC0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     284-290: ITTGYNL → VKISSWG
     291-594: Missing.

Show »
Length:162
Mass (Da):18,731
Checksum:iE282E87B51F209C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321F → S in AAS82953 (PubMed:16008511).Curated1
Sequence conflicti321F → S in BAB27964 (PubMed:16141072).Curated1
Sequence conflicti460R → Q in BAC27215 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211941 – 351Missing in isoform 3. 1 PublicationAdd BLAST351
Alternative sequenceiVSP_0211951 – 128Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST128
Alternative sequenceiVSP_021196226 – 283Missing in isoform 2. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_021197284 – 290ITTGYNL → VKISSWG in isoform 4. 1 Publication7
Alternative sequenceiVSP_021198291 – 594Missing in isoform 4. 1 PublicationAdd BLAST304

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513271 mRNA. Translation: AAS82953.1.
AK011993 mRNA. Translation: BAB27964.1.
AK029403 mRNA. Translation: BAC26435.1.
AK031017 mRNA. Translation: BAC27215.1.
AK031371 mRNA. Translation: BAC27371.1.
AK146777 mRNA. Translation: BAE27425.1.
AK166570 mRNA. Translation: BAE38861.1.
BC016123 mRNA. Translation: AAH16123.1.
BC019973 mRNA. Translation: AAH19973.1.
BC057968 mRNA. Translation: AAH57968.1.
CCDSiCCDS26159.1. [Q91WC0-1]
RefSeqiNP_082538.2. NM_028262.3. [Q91WC0-1]
XP_006516146.1. XM_006516083.3. [Q91WC0-2]
UniGeneiMm.159185.
Mm.472969.

Genome annotation databases

EnsembliENSMUST00000071095; ENSMUSP00000066413; ENSMUSG00000056770. [Q91WC0-1]
GeneIDi52690.
KEGGimmu:52690.
UCSCiuc007ozj.2. mouse. [Q91WC0-1]
uc007ozk.2. mouse. [Q91WC0-2]
uc007ozl.2. mouse. [Q91WC0-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513271 mRNA. Translation: AAS82953.1.
AK011993 mRNA. Translation: BAB27964.1.
AK029403 mRNA. Translation: BAC26435.1.
AK031017 mRNA. Translation: BAC27215.1.
AK031371 mRNA. Translation: BAC27371.1.
AK146777 mRNA. Translation: BAE27425.1.
AK166570 mRNA. Translation: BAE38861.1.
BC016123 mRNA. Translation: AAH16123.1.
BC019973 mRNA. Translation: AAH19973.1.
BC057968 mRNA. Translation: AAH57968.1.
CCDSiCCDS26159.1. [Q91WC0-1]
RefSeqiNP_082538.2. NM_028262.3. [Q91WC0-1]
XP_006516146.1. XM_006516083.3. [Q91WC0-2]
UniGeneiMm.159185.
Mm.472969.

3D structure databases

ProteinModelPortaliQ91WC0.
SMRiQ91WC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206736. 6 interactors.
IntActiQ91WC0. 6 interactors.
STRINGi10090.ENSMUSP00000066413.

PTM databases

iPTMnetiQ91WC0.
PhosphoSitePlusiQ91WC0.

Proteomic databases

EPDiQ91WC0.
PaxDbiQ91WC0.
PeptideAtlasiQ91WC0.
PRIDEiQ91WC0.

Protocols and materials databases

DNASUi52690.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071095; ENSMUSP00000066413; ENSMUSG00000056770. [Q91WC0-1]
GeneIDi52690.
KEGGimmu:52690.
UCSCiuc007ozj.2. mouse. [Q91WC0-1]
uc007ozk.2. mouse. [Q91WC0-2]
uc007ozl.2. mouse. [Q91WC0-4]

Organism-specific databases

CTDi84193.
MGIiMGI:1289184. Setd3.

Phylogenomic databases

eggNOGiKOG1337. Eukaryota.
ENOG410Y7DR. LUCA.
GeneTreeiENSGT00850000132334.
HOGENOMiHOG000049107.
HOVERGENiHBG062823.
InParanoidiQ91WC0.
KOiK19199.
OMAiNGTRSEN.
OrthoDBiEOG091G087D.
PhylomeDBiQ91WC0.
TreeFamiTF354226.

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiSetd3. mouse.
PROiQ91WC0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056770.
CleanExiMM_SETD3.
ExpressionAtlasiQ91WC0. baseline and differential.
GenevisibleiQ91WC0. MM.

Family and domain databases

Gene3Di3.90.1420.10. 1 hit.
InterProiIPR025785. Hist-Lys_N-MeTrfase_SETD3.
IPR015353. Rubisco_LSMT_subst-bd.
IPR001214. SET_dom.
[Graphical view]
PfamiPF09273. Rubis-subs-bind. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SUPFAMiSSF81822. SSF81822. 1 hit.
PROSITEiPS51565. SAM_MT43_SETD3. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSETD3_MOUSE
AccessioniPrimary (citable) accession number: Q91WC0
Secondary accession number(s): Q6PEN3
, Q8CD86, Q8CDX8, Q9CZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.