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Protein

Histone-lysine N-methyltransferase setd3

Gene

Setd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3 (H3K4me and H3K36me) (PubMed:21832073). Acts as a transcriptional activator (PubMed:21832073). Plays an important role in the transcriptional regulation of muscle cell differentiation via interaction with MYOD1 (PubMed:21832073).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].1 Publication

GO - Molecular functioni

  • histone methyltransferase activity (H3-K36 specific) Source: MGI
  • histone methyltransferase activity (H3-K4 specific) Source: MGI
  • RNA polymerase II activating transcription factor binding Source: MGI
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase setd3 (EC:2.1.1.431 Publication)
Alternative name(s):
Endothelial differentiation inhibitory protein D10
SET domain-containing protein 3
Gene namesi
Name:Setd3
Synonyms:D12Ertd771e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1289184 Setd3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002541761 – 594Histone-lysine N-methyltransferase setd3Add BLAST594

Proteomic databases

EPDiQ91WC0
PaxDbiQ91WC0
PeptideAtlasiQ91WC0
PRIDEiQ91WC0

PTM databases

iPTMnetiQ91WC0
PhosphoSitePlusiQ91WC0

Expressioni

Tissue specificityi

Prominently expressed in the heart and skeletal muscles and is also detected weakly in the stomach, small intestine, and colon.1 Publication

Gene expression databases

BgeeiENSMUSG00000056770
CleanExiMM_SETD3
ExpressionAtlasiQ91WC0 baseline and differential
GenevisibleiQ91WC0 MM

Interactioni

Subunit structurei

Interacts with MYOD1.1 Publication

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi206736, 6 interactors
IntActiQ91WC0, 6 interactors
STRINGi10090.ENSMUSP00000066413

Structurei

3D structure databases

ProteinModelPortaliQ91WC0
SMRiQ91WC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 314SETPROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1337 Eukaryota
ENOG410Y7DR LUCA
GeneTreeiENSGT00910000144155
HOGENOMiHOG000049107
HOVERGENiHBG062823
InParanoidiQ91WC0
KOiK19199
OMAiCERADPN
OrthoDBiEOG091G087D
PhylomeDBiQ91WC0
TreeFamiTF354226

Family and domain databases

Gene3Di3.90.1420.10, 1 hit
InterProiView protein in InterPro
IPR025785 Hist-Lys_N-MeTrfase_SETD3
IPR015353 Rubisco_LSMT_subst-bd
IPR036464 Rubisco_LSMT_subst-bd_sf
IPR001214 SET_dom
PfamiView protein in Pfam
PF09273 Rubis-subs-bind, 1 hit
PF00856 SET, 1 hit
SUPFAMiSSF81822 SSF81822, 1 hit
PROSITEiView protein in PROSITE
PS51565 SAM_MT43_SETD3, 1 hit
PS50280 SET, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKSRVKTQ KSGTGATATV SPKEILNLTS ELLQKCSSPA PSPGKEWEEY
60 70 80 90 100
TQIRALVEKI RKKQKGLSVT FDGKREDYFP DLMKWASENG ASVEGFEMVN
110 120 130 140 150
FKEEGFGLRA TRDIKAEELF LWVPRKLLMT VESAKNSVLG PLYSQDRILQ
160 170 180 190 200
AMGNIALAFH LLCERASPNS FWQPYIQTLP SEYDTPLYFE EEEVRCLQST
210 220 230 240 250
QAIHDVFSQY KNTARQYAYF YKVIQTHPHA NKLPLKESFT YEDYRWAVSS
260 270 280 290 300
VMTRQNQIPT EDGSRVTLAL IPLWDMCNHT NGLITTGYNL EDDRCECVAL
310 320 330 340 350
QDFQAGDQIY IFYGTRSNAE FVIHSGFFFD NNSHDRVKIK LGVSKSDRLY
360 370 380 390 400
AMKAEVLARA GIPTSSVFAL HSTEPPISAQ LLAFLRVFCM TEEELKEHLL
410 420 430 440 450
GDSAIDRIFT LGNAEFPVSW DNEVKLWTFL EDRASLLLKT YKTTIEEDKI
460 470 480 490 500
VLKNPDLSVR ATMAIKLRLG EKEILEKAVK SAAVNREYYR KHMEERAPLP
510 520 530 540 550
RYEESDLGLL EGGVGDSRLP LVLRKLEEEA GVQESLSLTE TVSKVKAAEN
560 570 580 590
GLVNGENLIP NGTRSENESL SPEESENVTG EESSGSMAKV KERL
Length:594
Mass (Da):67,176
Last modified:December 1, 2001 - v1
Checksum:i67DA47889A5C9F55
GO
Isoform 2 (identifier: Q91WC0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     226-283: Missing.

Show »
Length:408
Mass (Da):46,072
Checksum:i051A8DA6EA0643BA
GO
Isoform 3 (identifier: Q91WC0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-351: Missing.

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,001
Checksum:iC3FD156DC6D07D43
GO
Isoform 4 (identifier: Q91WC0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     284-290: ITTGYNL → VKISSWG
     291-594: Missing.

Show »
Length:162
Mass (Da):18,731
Checksum:iE282E87B51F209C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321F → S in AAS82953 (PubMed:16008511).Curated1
Sequence conflicti321F → S in BAB27964 (PubMed:16141072).Curated1
Sequence conflicti460R → Q in BAC27215 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211941 – 351Missing in isoform 3. 1 PublicationAdd BLAST351
Alternative sequenceiVSP_0211951 – 128Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST128
Alternative sequenceiVSP_021196226 – 283Missing in isoform 2. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_021197284 – 290ITTGYNL → VKISSWG in isoform 4. 1 Publication7
Alternative sequenceiVSP_021198291 – 594Missing in isoform 4. 1 PublicationAdd BLAST304

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513271 mRNA Translation: AAS82953.1
AK011993 mRNA Translation: BAB27964.1
AK029403 mRNA Translation: BAC26435.1
AK031017 mRNA Translation: BAC27215.1
AK031371 mRNA Translation: BAC27371.1
AK146777 mRNA Translation: BAE27425.1
AK166570 mRNA Translation: BAE38861.1
BC016123 mRNA Translation: AAH16123.1
BC019973 mRNA Translation: AAH19973.1
BC057968 mRNA Translation: AAH57968.1
CCDSiCCDS26159.1 [Q91WC0-1]
RefSeqiNP_082538.2, NM_028262.3 [Q91WC0-1]
XP_006516146.1, XM_006516083.3 [Q91WC0-2]
UniGeneiMm.159185
Mm.472969

Genome annotation databases

EnsembliENSMUST00000071095; ENSMUSP00000066413; ENSMUSG00000056770 [Q91WC0-1]
GeneIDi52690
KEGGimmu:52690
UCSCiuc007ozj.2 mouse [Q91WC0-1]
uc007ozk.2 mouse [Q91WC0-2]
uc007ozl.2 mouse [Q91WC0-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSETD3_MOUSE
AccessioniPrimary (citable) accession number: Q91WC0
Secondary accession number(s): Q6PEN3
, Q8CD86, Q8CDX8, Q9CZZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health