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Protein

TIMELESS-interacting protein

Gene

Tipin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress.1 Publication

GO - Biological processi

  • cell cycle phase transition Source: BHF-UCL
  • cell division Source: UniProtKB-KW
  • DNA damage checkpoint Source: MGI
  • DNA replication checkpoint Source: UniProtKB
  • intra-S DNA damage checkpoint Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • replication fork protection Source: InterPro
  • response to UV Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-5693607. Processing of DNA double-strand break ends.

Names & Taxonomyi

Protein namesi
Recommended name:
TIMELESS-interacting protein
Gene namesi
Name:Tipin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1921571. Tipin.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278TIMELESS-interacting proteinPRO_0000305254Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphoserineBy similarity
Modified residuei233 – 2331PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91WA1.
MaxQBiQ91WA1.
PaxDbiQ91WA1.
PRIDEiQ91WA1.

PTM databases

PhosphoSiteiQ91WA1.

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Developmental stagei

Expression peaks between E11 and E15. At E7.5, expressed in germ cell layers. At E14.5, expressed at highest levels in thymus, liver, gastrointestinal tract, lung and the rapidly proliferating ventricular zone of the brain.1 Publication

Gene expression databases

BgeeiQ91WA1.
CleanExiMM_TIPIN.
GenevisibleiQ91WA1. MM.

Interactioni

Subunit structurei

Interacts with MCM6 and MCM7 (By similarity). Interacts with TIMELESS, which impairs TIMELESS self-association. Interacts with RPA2 and PRDX2.By similarity2 Publications

Protein-protein interaction databases

BioGridi211238. 30 interactions.
IntActiQ91WA1. 28 interactions.
STRINGi10090.ENSMUSP00000034964.

Structurei

3D structure databases

ProteinModelPortaliQ91WA1.
SMRiQ91WA1. Positions 192-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 14077Interaction with TIMELESSBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CSM3 family.Curated

Phylogenomic databases

eggNOGiKOG3004. Eukaryota.
ENOG4111VXN. LUCA.
GeneTreeiENSGT00390000005764.
HOGENOMiHOG000154594.
HOVERGENiHBG061130.
InParanoidiQ91WA1.
KOiK10904.
OMAiDILDNPC.
OrthoDBiEOG7MSMPZ.
PhylomeDBiQ91WA1.
TreeFamiTF313290.

Family and domain databases

InterProiIPR012923. Csm3.
[Graphical view]
PfamiPF07962. Swi3. 1 hit.
[Graphical view]
ProDomiPD089639. Swi3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q91WA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEQEENGLF EIPDYEHVED ETFPPFPPPA SPERDPADAE PEEGSGSGVP
60 70 80 90 100
VPPKRTVKRN LPKLDATRLT SERGLPALRH VFDKTKFKGK GHEAEDLKTL
110 120 130 140 150
IRHMEHWAHR LFPKLQFEDF IDRVENLGNK KEVQTCLKRI RLDLPIVHED
160 170 180 190 200
FVNNNDEVGE ANGPDVSATG FDPFLTSSSD SRKFASEPTR SLTEEQQQRI
210 220 230 240 250
ERNKQLALER RQAKLLSNSQ SLENDVTVEE NSTGEDQEES NGLNIDTADG
260 270
PHDVPFASTH EEEQCKAEET QLDHTNLD
Length:278
Mass (Da):31,498
Last modified:October 2, 2007 - v2
Checksum:i769F56BBDF2763C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671T → A in BAB22798 (PubMed:16141072).Curated
Sequence conflicti265 – 2651C → F in AAH16211 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003451 mRNA. Translation: BAB22798.1.
AK003802 mRNA. Translation: BAB23004.1.
AK011357 mRNA. Translation: BAB27565.1.
AK038111 mRNA. Translation: BAC29931.1.
BC016211 mRNA. Translation: AAH16211.1.
BK001385 mRNA. Translation: DAA01364.1.
CCDSiCCDS23279.1.
RefSeqiNP_079648.1. NM_025372.3.
XP_006511400.1. XM_006511337.2.
XP_006511401.1. XM_006511338.2.
XP_006511402.1. XM_006511339.2.
XP_006511403.1. XM_006511340.2.
UniGeneiMm.196219.

Genome annotation databases

EnsembliENSMUST00000034964; ENSMUSP00000034964; ENSMUSG00000032397.
GeneIDi66131.
KEGGimmu:66131.
UCSCiuc009qbr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003451 mRNA. Translation: BAB22798.1.
AK003802 mRNA. Translation: BAB23004.1.
AK011357 mRNA. Translation: BAB27565.1.
AK038111 mRNA. Translation: BAC29931.1.
BC016211 mRNA. Translation: AAH16211.1.
BK001385 mRNA. Translation: DAA01364.1.
CCDSiCCDS23279.1.
RefSeqiNP_079648.1. NM_025372.3.
XP_006511400.1. XM_006511337.2.
XP_006511401.1. XM_006511338.2.
XP_006511402.1. XM_006511339.2.
XP_006511403.1. XM_006511340.2.
UniGeneiMm.196219.

3D structure databases

ProteinModelPortaliQ91WA1.
SMRiQ91WA1. Positions 192-217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211238. 30 interactions.
IntActiQ91WA1. 28 interactions.
STRINGi10090.ENSMUSP00000034964.

PTM databases

PhosphoSiteiQ91WA1.

Proteomic databases

EPDiQ91WA1.
MaxQBiQ91WA1.
PaxDbiQ91WA1.
PRIDEiQ91WA1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034964; ENSMUSP00000034964; ENSMUSG00000032397.
GeneIDi66131.
KEGGimmu:66131.
UCSCiuc009qbr.1. mouse.

Organism-specific databases

CTDi54962.
MGIiMGI:1921571. Tipin.

Phylogenomic databases

eggNOGiKOG3004. Eukaryota.
ENOG4111VXN. LUCA.
GeneTreeiENSGT00390000005764.
HOGENOMiHOG000154594.
HOVERGENiHBG061130.
InParanoidiQ91WA1.
KOiK10904.
OMAiDILDNPC.
OrthoDBiEOG7MSMPZ.
PhylomeDBiQ91WA1.
TreeFamiTF313290.

Enzyme and pathway databases

ReactomeiR-MMU-5693607. Processing of DNA double-strand break ends.

Miscellaneous databases

NextBioi320724.
PROiQ91WA1.
SOURCEiSearch...

Gene expression databases

BgeeiQ91WA1.
CleanExiMM_TIPIN.
GenevisibleiQ91WA1. MM.

Family and domain databases

InterProiIPR012923. Csm3.
[Graphical view]
PfamiPF07962. Swi3. 1 hit.
[Graphical view]
ProDomiPD089639. Swi3. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  3. "Tipin, a novel timeless-interacting protein, is developmentally co-expressed with timeless and disrupts its self-association."
    Gotter A.L.
    J. Mol. Biol. 331:167-176(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, INTERACTION WITH TIMELESS, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  4. "Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors."
    Gotter A.L., Suppa C., Emanuel B.S.
    J. Mol. Biol. 366:36-52(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPA2 AND PRDX2, FUNCTION.

Entry informationi

Entry nameiTIPIN_MOUSE
AccessioniPrimary (citable) accession number: Q91WA1
Secondary accession number(s): Q9CQM3, Q9D1J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 13, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.