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Protein

Alpha-mannosidase 2C1

Gene

Man2c1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues from glycoproteins. Involved in the degradation of free oligosaccharides in the cytoplasm.2 Publications

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.2 Publications

Cofactori

Co2+1 Publication

Enzyme regulationi

Inhibited by 1,4-dideoxy-1,4-imino-d-mannitol (DIM) and EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CobaltCurated1
Metal bindingi261CobaltCurated1
Active sitei371NucleophileBy similarity1
Metal bindingi371CobaltCurated1
Metal bindingi576CobaltCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCobalt, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8853383. Lysosomal oligosaccharide catabolism.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2C1 (EC:3.2.1.242 Publications)
Alternative name(s):
Alpha-D-mannoside mannohydrolase
Mannosidase alpha class 2C member 1
Neutral/cytosolic alpha-mannosidase1 Publication
Gene namesi
Name:Man2c11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920994. Man2c1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Viable and fertile with no gross defects. Loss of neutral mannosidase activity leading to accumulation of free higher-order oligosaccharides such as Man8-9GlcNAc1 in many organs, particularly liver and heart. Tissues show histopathological changes with strongest defects observed in liver, small intestine, kidney and central nervous system (CNS). In liver, hepatocytes appear swollen with increased levels of glycogen and accumulation of lipid droplets. In the small intestine, enterocytes accumulate glycogen apically and also develop vacuoles in the basal cell region. In the CNS, neurons in isocortex lamina V show signs of degeneration with formation of vacuoles in basal cell regions. Vacuolation is also found in glial cells of white matter tracts. In kidney, there are signs of fibrosis along Bowman's capsule and a small number of glomeruli appear to be collapsed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002069081 – 1039Alpha-mannosidase 2C1Add BLAST1039

Proteomic databases

EPDiQ91W89.
MaxQBiQ91W89.
PaxDbiQ91W89.
PRIDEiQ91W89.

PTM databases

iPTMnetiQ91W89.
PhosphoSitePlusiQ91W89.

Expressioni

Tissue specificityi

Expressed in kidney and liver (at protein level) (PubMed:24550399). Widely expressed, with highest levels in lung, ovary and testis (PubMed:16904268). Also detected at lower levels in heart, brain, liver, spleen, kidney and thymus (PubMed:16904268).2 Publications

Gene expression databases

BgeeiENSMUSG00000032295.
CleanExiMM_MAN2C1.
ExpressionAtlasiQ91W89. baseline and differential.
GenevisibleiQ91W89. MM.

Interactioni

Protein-protein interaction databases

IntActiQ91W89. 1 interactor.
MINTiMINT-4100968.
STRINGi10090.ENSMUSP00000125478.

Structurei

3D structure databases

ProteinModelPortaliQ91W89.
SMRiQ91W89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Phylogenomic databases

eggNOGiKOG4342. Eukaryota.
COG0383. LUCA.
GeneTreeiENSGT00390000006338.
HOGENOMiHOG000048425.
HOVERGENiHBG055567.
InParanoidiQ91W89.
KOiK01191.
OMAiGQYWDAW.
OrthoDBiEOG091G016H.
PhylomeDBiQ91W89.
TreeFamiTF300335.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
3.20.110.10. 1 hit.
InterProiView protein in InterPro
IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
PfamiView protein in Pfam
PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
SMARTiView protein in SMART
SM00872. Alpha-mann_mid. 1 hit.
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91W89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAPFLKHW RTTFERVEKF VSPIYFTDCN LRGRLFGDSC SVTLSSFLTP
60 70 80 90 100
ERLPYEKAVQ QNFSPAQVGD SFGPTWWTCW FRVELVIPEV WVGQEVHLCW
110 120 130 140 150
ESDGESLVWR DGEPVQGLTK EGEKTSYVLS ERLRASDPRS LTLYVEVACN
160 170 180 190 200
GLLGAGKGSM IAAPDPEKMF QLSQAKLAVF HRDVHSLLVD LELLLGVAKG
210 220 230 240 250
LGEDSQRSFQ ALHTANQMVN ICDPAQPETY PAAKALASKF FGQHGGESQH
260 270 280 290 300
TIHAMGHCHI DTAWLWPFKE TVRKCARSWS TAVTLMEQNT DFIFACSQAQ
310 320 330 340 350
QLEWVKSQYP GLHARLQEFA CRGQFVPVGG TWVEMDGNLP SGEAMVRQFL
360 370 380 390 400
QGQNFFLQEF GKMCSEFWLP DTFGYSAQLP QIMQGCGIKR FLTQKLSWNL
410 420 430 440 450
VNSFPHHTFF WEGLDGSRVL VHFPPGDSYG MQGSVEEVLK TVTNNRDKGR
460 470 480 490 500
TNHSGFLFGF GDGGGGPTQT MLDRLKRLSN TDGLPRVQLS SPGQLFTALE
510 520 530 540 550
RDSGQLCTWV GELFLELHNG TYTTHAQLKK GNRECEQILH DVEVLSSLAL
560 570 580 590 600
ARSAQFLYPA AQLQHLWRLL LLNQFHDVVT GSCIQLVAED AMNYYEDIRS
610 620 630 640 650
HGNPLLSAAA AALCAGEPGP KGLLIINTLP WKRTEVLALP KPCGAHSLAL
660 670 680 690 700
VTVPSIGYAP APTPTSLQPL LPQQPVFVMQ ETDGSVTLDN GIIRVRLDPT
710 720 730 740 750
GCLTSLVLVA SGREAIAEGA LGNQFVLFDD VPLYWDAWDV MDYHLETRKP
760 770 780 790 800
VLGQAGTLAV GTEGGLRGSA WFLLQISPNS RLSQEVVLDV GCPYVRFHTE
810 820 830 840 850
VHWHEAHKFL KVEFPARIRS PQATYEIQFG HLQRPTHNNT SWDWARYEVW
860 870 880 890 900
AHRWIDLSEC DFGLALLNNC KYGASVRGNV LSLSLLRAPK APDATADMGR
910 920 930 940 950
HEFTYALMPH KGSFQEAGVI HAAYNLNFPL LALPAPGPAP DTTWSAFSVS
960 970 980 990 1000
SPAVVLETIK QAERCHQHRT LVLRLYEAHG SHVDCWLHTS LPVQEATLCD
1010 1020 1030
LLEQRDPTGH LSLQDNRLKL TFSPFQVRSL LLVLQSPPN
Length:1,039
Mass (Da):115,688
Last modified:December 1, 2001 - v1
Checksum:i308F2F841590F9E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AH014888
, AY996589, AY996590, AY996591, AY996592, AY996593, AY996594, AY996595, AY996597, AY996598, AY996599, AY996600, AY996601, AY996602, AY996603, AY996604, AY996605, AY996606, AY996607, AY996608, AY996609, AY996610, AY996611, AY996612, AY996613 Genomic DNA. Translation: AAY53557.1.
AK153935 mRNA. Translation: BAE32264.1.
CH466522 Genomic DNA. Translation: EDL25887.1.
BC016253 mRNA. Translation: AAH16253.1.
CCDSiCCDS40646.1.
RefSeqiNP_082912.1. NM_028636.2.
UniGeneiMm.30110.
Mm.474792.
Mm.488131.

Genome annotation databases

EnsembliENSMUST00000160147; ENSMUSP00000125478; ENSMUSG00000032295.
GeneIDi73744.
KEGGimmu:73744.
UCSCiuc009pud.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMA2C1_MOUSE
AccessioniPrimary (citable) accession number: Q91W89
Secondary accession number(s): Q3ZCX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: December 1, 2001
Last modified: July 5, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families