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Protein

Vacuolar protein sorting-associated protein 11 homolog

Gene

Vps11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri822 – 86140RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 11 homolog
Gene namesi
Name:Vps11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918982. Vps11.

Subcellular locationi

GO - Cellular componenti

  • actin filament Source: MGI
  • autophagosome Source: UniProtKB-SubCell
  • clathrin-coated vesicle Source: UniProtKB-SubCell
  • CORVET complex Source: UniProtKB
  • early endosome Source: MGI
  • endocytic vesicle Source: UniProtKB
  • endosome Source: MGI
  • HOPS complex Source: MGI
  • late endosome Source: MGI
  • late endosome membrane Source: UniProtKB-SubCell
  • lysosomal membrane Source: MGI
  • lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 941940Vacuolar protein sorting-associated protein 11 homologPRO_0000055903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei813 – 8131PhosphoserineBy similarity
Modified residuei924 – 9241PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ91W86.
MaxQBiQ91W86.
PaxDbiQ91W86.
PeptideAtlasiQ91W86.
PRIDEiQ91W86.

PTM databases

iPTMnetiQ91W86.
PhosphoSiteiQ91W86.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032127.
CleanExiMM_VPS11.
GenevisibleiQ91W86. MM.

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuaole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:25266290). Interacts with RAB5C (PubMed:25266290). Interacts with TGFBRAP1, MON1B, STX7, STX17, ECM29, EZR, RDX, MSN (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).By similarity1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VIPAS39Q9H9C13EBI-2527812,EBI-749080From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214887. 10 interactions.
IntActiQ91W86. 3 interactions.
STRINGi10090.ENSMUSP00000034644.

Structurei

3D structure databases

ProteinModelPortaliQ91W86.
SMRiQ91W86. Positions 140-218, 820-864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati411 – 561151CHCR 1Add
BLAST
Repeati572 – 736165CHCR 2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili772 – 81342Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the VPS11 family.Curated
Contains 2 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri822 – 86140RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2114. Eukaryota.
ENOG410XRNZ. LUCA.
GeneTreeiENSGT00390000015058.
HOGENOMiHOG000216424.
HOVERGENiHBG055822.
InParanoidiQ91W86.
KOiK20179.
OMAiQHSILVS.
OrthoDBiEOG091G04NG.
TreeFamiTF300479.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016528. VPS11.
IPR024763. VPS11_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23323:SF24. PTHR23323:SF24. 1 hit.
PfamiPF00637. Clathrin. 1 hit.
PF12451. VPS11_C. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50236. CHCR. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91W86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAYLQWRRF VFFEKELVKE PLGNDGAAPG AAPVSGSAAS KFLCLPPGIT
60 70 80 90 100
VCDSGRGSLV FGDMEGQIWF LPRSLQLTGF QAYKLRVTHL YQLKQHNILA
110 120 130 140 150
SVGEDEEGIN PLVKIWNLEK RDGGNPLCTR IFPAIPGTEP TVVSCLTVHE
160 170 180 190 200
NLNFMAIGFT DGSVTLNKGD ITRDRHSKTQ ILHKGNYPVT GLAFRQAGKT
210 220 230 240 250
THLFVVTTEN VQSYIVSGKD YPRVELDTHG CGLRCSALSD PSQDLQFIVA
260 270 280 290 300
GDECVYLYQP DERGPCFAFE GHKLIVHWFR GYLVIVSRDR KVSPKSEFTS
310 320 330 340 350
RDSQNSDKQI LNIYDLCNKF IAYSAGFEDI VDVLAEWGSL YVLTRDGRVH
360 370 380 390 400
ALQEKDTQTK LEMLFKKNLF EMAINLAKSQ HLDSDGLAQI FMQYGDHLYS
410 420 430 440 450
KGNHDGAVQQ YIRTIGKLEP SYVIRKFLDA QRIHNLTAYL QTLHRQSLAN
460 470 480 490 500
ADHTTLLLNC YTKLKDSSKL EEFIKTKSES EVHFDVETAI KVLRQAGYYS
510 520 530 540 550
HALYLAENHA HHEWYLKIQL EDIKNYQEAL RYIGKLPFEQ AESNMKRYGK
560 570 580 590 600
TLMHHIPEQT TQLLKGLCTD YRPSLEGRGD REALSCRASS EEFIPIFANN
610 620 630 640 650
PRELKAFLEH MSEVQPDSPQ GIYDTLLELR LQNWAHEKDP QAKEKLHAEA
660 670 680 690 700
ISLLKSGRFC DVFDKALVLC QMHDFQDGVL YLYEQGKLFQ QIMHYHMQHE
710 720 730 740 750
QYRQVIAVCE RHGEQEPSLW EQALSYFARK EEDCKEYVAA VLRHIENKSL
760 770 780 790 800
MPPLLVVQTL AHNSTATLSI IRDYLVQKLQ KQSQQIAQDE LRVRRYREET
810 820 830 840 850
TRIRQEIQEL KASPKIFQKT KCSICNSALE LPSVHFLCGH SFHQHCFESY
860 870 880 890 900
SESDADCPTC LPENRKVMDM IRAQEQKRDL HDQFQHQLKC SNDSFSVIAD
910 920 930 940
YFGRGVFNKL TLLTDPPTAR LTPSLEAGLQ RDLLMHSRRG T
Length:941
Mass (Da):107,719
Last modified:July 27, 2011 - v3
Checksum:iDB96F07E92C9E534
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → HAS in AAH16258 (PubMed:15489334).Curated
Sequence conflicti28 – 281A → T in AAH16258 (PubMed:15489334).Curated
Sequence conflicti34 – 341V → T in AAH16258 (PubMed:15489334).Curated
Sequence conflicti37 – 382SA → PT in AAH16258 (PubMed:15489334).Curated
Sequence conflicti132 – 1321F → L in AAH16258 (PubMed:15489334).Curated
Sequence conflicti186 – 1861N → S in AAH16258 (PubMed:15489334).Curated
Sequence conflicti276 – 2761V → A in AAH16258 (PubMed:15489334).Curated
Sequence conflicti326 – 3261G → V in AAH16258 (PubMed:15489334).Curated
Sequence conflicti330 – 3301I → V in AAH16258 (PubMed:15489334).Curated
Sequence conflicti551 – 5511T → I in AAH16258 (PubMed:15489334).Curated
Sequence conflicti583 – 5842AL → FP in AAH16258 (PubMed:15489334).Curated
Sequence conflicti589 – 5891S → N in AAH16258 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004695 mRNA. Translation: BAB23481.1.
BC029004 mRNA. Translation: AAH29004.1.
BC016258 mRNA. Translation: AAH16258.1.
CCDSiCCDS40599.1.
RefSeqiNP_082165.1. NM_027889.1.
UniGeneiMm.295013.

Genome annotation databases

EnsembliENSMUST00000034644; ENSMUSP00000034644; ENSMUSG00000032127.
GeneIDi71732.
KEGGimmu:71732.
UCSCiuc009pdc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004695 mRNA. Translation: BAB23481.1.
BC029004 mRNA. Translation: AAH29004.1.
BC016258 mRNA. Translation: AAH16258.1.
CCDSiCCDS40599.1.
RefSeqiNP_082165.1. NM_027889.1.
UniGeneiMm.295013.

3D structure databases

ProteinModelPortaliQ91W86.
SMRiQ91W86. Positions 140-218, 820-864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214887. 10 interactions.
IntActiQ91W86. 3 interactions.
STRINGi10090.ENSMUSP00000034644.

PTM databases

iPTMnetiQ91W86.
PhosphoSiteiQ91W86.

Proteomic databases

EPDiQ91W86.
MaxQBiQ91W86.
PaxDbiQ91W86.
PeptideAtlasiQ91W86.
PRIDEiQ91W86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034644; ENSMUSP00000034644; ENSMUSG00000032127.
GeneIDi71732.
KEGGimmu:71732.
UCSCiuc009pdc.2. mouse.

Organism-specific databases

CTDi55823.
MGIiMGI:1918982. Vps11.

Phylogenomic databases

eggNOGiKOG2114. Eukaryota.
ENOG410XRNZ. LUCA.
GeneTreeiENSGT00390000015058.
HOGENOMiHOG000216424.
HOVERGENiHBG055822.
InParanoidiQ91W86.
KOiK20179.
OMAiQHSILVS.
OrthoDBiEOG091G04NG.
TreeFamiTF300479.

Miscellaneous databases

PROiQ91W86.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032127.
CleanExiMM_VPS11.
GenevisibleiQ91W86. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016528. VPS11.
IPR024763. VPS11_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23323:SF24. PTHR23323:SF24. 1 hit.
PfamiPF00637. Clathrin. 1 hit.
PF12451. VPS11_C. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50236. CHCR. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS11_MOUSE
AccessioniPrimary (citable) accession number: Q91W86
Secondary accession number(s): Q5FWZ1, Q9DBX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.