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Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

Gldc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH) (By similarity).By similarity

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.

Cofactori

Enzyme regulationi

Stimulated by lipoic acid. Inhibited in presence of methylamine (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
Alternative name(s):
Glycine cleavage system P protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene namesi
Name:Gldc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1341155. Gldc.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionSequence AnalysisAdd
BLAST
Chaini36 – 1025990Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei452 – 4521N6-acetyllysine1 Publication
Modified residuei519 – 5191N6-acetyllysine1 Publication
Modified residuei653 – 6531N6-acetyllysine1 Publication
Modified residuei669 – 6691N6-acetyllysine1 Publication
Modified residuei759 – 7591N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ91W43.
PaxDbiQ91W43.
PRIDEiQ91W43.

PTM databases

PhosphoSiteiQ91W43.

Expressioni

Gene expression databases

BgeeiQ91W43.
CleanExiMM_GLDC.
GenevisibleiQ91W43. MM.

Interactioni

Subunit structurei

Interacts with GCSH (By similarity). Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH) (By similarity).By similarity

Protein-protein interaction databases

BioGridi222425. 1 interaction.
IntActiQ91W43. 1 interaction.
MINTiMINT-1847717.
STRINGi10090.ENSMUSP00000025778.

Structurei

3D structure databases

ProteinModelPortaliQ91W43.
SMRiQ91W43. Positions 51-1010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1003.
GeneTreeiENSGT00390000017970.
HOGENOMiHOG000239369.
HOVERGENiHBG005820.
InParanoidiQ91W43.
KOiK00281.
OMAiRAESEMT.
OrthoDBiEOG7FJGZQ.
PhylomeDBiQ91W43.
TreeFamiTF300678.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR003437. GcvP.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91W43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLCARAWGL RLGRGAGGGH RLARGTGLSW AQRSRDSSGG GGGGGGGDRG
60 70 80 90 100
AAGASRLLER LLPRHDDFSR RHIGPGDKDR REMLQALGLA SIDELIEKTV
110 120 130 140 150
PASIRLKRPL KMEDPICENE ILETLHAIAS KNQIWRSYIG MGYYNCSVPQ
160 170 180 190 200
TILRNLLENS GWVTQYTPYQ PEVSQGRLES LLNYQTMVSD ITGLDMANAS
210 220 230 240 250
LLDEATAAAE AMQLCHRHNK RKKFFVDPRC HPQTIAVVQT RAKYRGVLVE
260 270 280 290 300
LKLPHEMDFS GKDVCGVLFQ YPDTEGKVED FTELVDRAHQ TGSLTCCATD
310 320 330 340 350
LLALCILRPP GEFGVDIALG NSQRFGVPLG YGGPHAAFFA VKENLVRMMP
360 370 380 390 400
GRMVGVTRDA TGKEVYRLAL QTREQHIRRD KATSNICTAQ ALLANMAAMF
410 420 430 440 450
AIYHGSQGLK HIAKRVHNAT LILSEGLKRA GHQLQHDLFF DTLKVQCGCS
460 470 480 490 500
VKEVLGRAAQ RQINFRLFDD GTLGISLDET VTEKDLDDLL WIFGCESSAE
510 520 530 540 550
LVAEGMGEER RGLLGSSFKR TSPFLTHQVF NSYHSETNLV RYMKKLENKD
560 570 580 590 600
ISLVHSMIPL GSCTMKLNSS SELAPITWRE FANIHPFVPL DQAQGYQQLF
610 620 630 640 650
QGLEKDLCEI TGYDRVSFQP NSGAQGEYAG LATIRAYLDQ KGERHRTVCL
660 670 680 690 700
IPKSAHGTNP ASAHMAGMKI QPVEVDRYGN IDVAHLKAMV DQHKENLAAI
710 720 730 740 750
MITYPSTNGV FEENIGDVCA LIHQHGGQVY LDGANMNAQV GICRPGDFGS
760 770 780 790 800
DVSHLNLHKT FCIPHGGGGP GMGPIGVKKH LSPFLPSHPV ISIKPTEGTW
810 820 830 840 850
PVGTVSAAPW GSSSILPISW AYIKMMGGKG LKEATEIAIL NANYMAKRLE
860 870 880 890 900
KHYRVLFRGA RGYVAHEFIL DTRPFKKSAN VEAVDVAKRL QDYGFHAPTM
910 920 930 940 950
SWPVAGTLMI EPTESEDKAE LDRFCDAMIS IRQEIADIEE GRIDPRVNPL
960 970 980 990 1000
KMSPHSLTCV TSSCWDRPYS REVAAFPLPF VKPENKFWPT IARIDDIYGD
1010 1020
QHLVCTCPPM EVYESPFSEQ KRASS
Length:1,025
Mass (Da):113,267
Last modified:December 1, 2001 - v1
Checksum:iBB4A1E3459FE3495
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017135 mRNA. Translation: AAH17135.1.
CCDSiCCDS37956.1.
RefSeqiNP_613061.1. NM_138595.2.
UniGeneiMm.274852.

Genome annotation databases

EnsembliENSMUST00000025778; ENSMUSP00000025778; ENSMUSG00000024827.
GeneIDi104174.
KEGGimmu:104174.
UCSCiuc008hej.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017135 mRNA. Translation: AAH17135.1.
CCDSiCCDS37956.1.
RefSeqiNP_613061.1. NM_138595.2.
UniGeneiMm.274852.

3D structure databases

ProteinModelPortaliQ91W43.
SMRiQ91W43. Positions 51-1010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222425. 1 interaction.
IntActiQ91W43. 1 interaction.
MINTiMINT-1847717.
STRINGi10090.ENSMUSP00000025778.

PTM databases

PhosphoSiteiQ91W43.

Proteomic databases

MaxQBiQ91W43.
PaxDbiQ91W43.
PRIDEiQ91W43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025778; ENSMUSP00000025778; ENSMUSG00000024827.
GeneIDi104174.
KEGGimmu:104174.
UCSCiuc008hej.1. mouse.

Organism-specific databases

CTDi2731.
MGIiMGI:1341155. Gldc.

Phylogenomic databases

eggNOGiCOG1003.
GeneTreeiENSGT00390000017970.
HOGENOMiHOG000239369.
HOVERGENiHBG005820.
InParanoidiQ91W43.
KOiK00281.
OMAiRAESEMT.
OrthoDBiEOG7FJGZQ.
PhylomeDBiQ91W43.
TreeFamiTF300678.

Miscellaneous databases

NextBioi356668.
PROiQ91W43.
SOURCEiSearch...

Gene expression databases

BgeeiQ91W43.
CleanExiMM_GLDC.
GenevisibleiQ91W43. MM.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR003437. GcvP.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye and Retina.
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 278-287; 606-615; 878-888 AND 933-942, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-452; LYS-519; LYS-653 AND LYS-669, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGCSP_MOUSE
AccessioniPrimary (citable) accession number: Q91W43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.