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Q91W43 (GCSP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine dehydrogenase (decarboxylating), mitochondrial

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene names
Name:Gldc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH) By similarity. HAMAP-Rule MF_00711

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00711

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00711

Enzyme regulation

Stimulated by lipoic acid. Inhibited in presence of methylamine By similarity. HAMAP-Rule MF_00711

Subunit structure

Interacts with GCSH By similarity. Homodimer. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH) By similarity. HAMAP-Rule MF_00711

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_00711.

Sequence similarities

Belongs to the GcvP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3535Mitochondrion Potential
Chain36 – 1025990Glycine dehydrogenase (decarboxylating), mitochondrial HAMAP-Rule MF_00711
PRO_0000010741

Amino acid modifications

Modified residue4521N6-acetyllysine Ref.3
Modified residue5191N6-acetyllysine Ref.3
Modified residue6531N6-acetyllysine Ref.3
Modified residue6691N6-acetyllysine Ref.3
Modified residue7591N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q91W43 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: BB4A1E3459FE3495

FASTA1,025113,267
        10         20         30         40         50         60 
MQLCARAWGL RLGRGAGGGH RLARGTGLSW AQRSRDSSGG GGGGGGGDRG AAGASRLLER 

        70         80         90        100        110        120 
LLPRHDDFSR RHIGPGDKDR REMLQALGLA SIDELIEKTV PASIRLKRPL KMEDPICENE 

       130        140        150        160        170        180 
ILETLHAIAS KNQIWRSYIG MGYYNCSVPQ TILRNLLENS GWVTQYTPYQ PEVSQGRLES 

       190        200        210        220        230        240 
LLNYQTMVSD ITGLDMANAS LLDEATAAAE AMQLCHRHNK RKKFFVDPRC HPQTIAVVQT 

       250        260        270        280        290        300 
RAKYRGVLVE LKLPHEMDFS GKDVCGVLFQ YPDTEGKVED FTELVDRAHQ TGSLTCCATD 

       310        320        330        340        350        360 
LLALCILRPP GEFGVDIALG NSQRFGVPLG YGGPHAAFFA VKENLVRMMP GRMVGVTRDA 

       370        380        390        400        410        420 
TGKEVYRLAL QTREQHIRRD KATSNICTAQ ALLANMAAMF AIYHGSQGLK HIAKRVHNAT 

       430        440        450        460        470        480 
LILSEGLKRA GHQLQHDLFF DTLKVQCGCS VKEVLGRAAQ RQINFRLFDD GTLGISLDET 

       490        500        510        520        530        540 
VTEKDLDDLL WIFGCESSAE LVAEGMGEER RGLLGSSFKR TSPFLTHQVF NSYHSETNLV 

       550        560        570        580        590        600 
RYMKKLENKD ISLVHSMIPL GSCTMKLNSS SELAPITWRE FANIHPFVPL DQAQGYQQLF 

       610        620        630        640        650        660 
QGLEKDLCEI TGYDRVSFQP NSGAQGEYAG LATIRAYLDQ KGERHRTVCL IPKSAHGTNP 

       670        680        690        700        710        720 
ASAHMAGMKI QPVEVDRYGN IDVAHLKAMV DQHKENLAAI MITYPSTNGV FEENIGDVCA 

       730        740        750        760        770        780 
LIHQHGGQVY LDGANMNAQV GICRPGDFGS DVSHLNLHKT FCIPHGGGGP GMGPIGVKKH 

       790        800        810        820        830        840 
LSPFLPSHPV ISIKPTEGTW PVGTVSAAPW GSSSILPISW AYIKMMGGKG LKEATEIAIL 

       850        860        870        880        890        900 
NANYMAKRLE KHYRVLFRGA RGYVAHEFIL DTRPFKKSAN VEAVDVAKRL QDYGFHAPTM 

       910        920        930        940        950        960 
SWPVAGTLMI EPTESEDKAE LDRFCDAMIS IRQEIADIEE GRIDPRVNPL KMSPHSLTCV 

       970        980        990       1000       1010       1020 
TSSCWDRPYS REVAAFPLPF VKPENKFWPT IARIDDIYGD QHLVCTCPPM EVYESPFSEQ 


KRASS 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye and Retina.
[2]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 278-287; 606-615; 878-888 AND 933-942, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[3]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-452; LYS-519; LYS-653 AND LYS-669, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC017135 mRNA. Translation: AAH17135.1.
RefSeqNP_613061.1. NM_138595.2.
UniGeneMm.274852.

3D structure databases

ProteinModelPortalQ91W43.
SMRQ91W43. Positions 51-1010.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ91W43. 1 interaction.
MINTMINT-1847717.
STRING10090.ENSMUSP00000025778.

PTM databases

PhosphoSiteQ91W43.

Proteomic databases

PaxDbQ91W43.
PRIDEQ91W43.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025778; ENSMUSP00000025778; ENSMUSG00000024827.
GeneID104174.
KEGGmmu:104174.
UCSCuc008hej.1. mouse.

Organism-specific databases

CTD2731.
MGIMGI:1341155. Gldc.

Phylogenomic databases

eggNOGCOG1003.
GeneTreeENSGT00390000017970.
HOGENOMHOG000239369.
HOVERGENHBG005820.
InParanoidQ91W43.
KOK00281.
OMAQTMVCDL.
OrthoDBEOG7FJGZQ.
PhylomeDBQ91W43.
TreeFamTF300678.

Gene expression databases

BgeeQ91W43.
CleanExMM_GLDC.
GenevestigatorQ91W43.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
HAMAPMF_00711. GcvP.
InterProIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF01212. Beta_elim_lyase. 1 hit.
PF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 3 hits.
TIGRFAMsTIGR00461. gcvP. 1 hit.
ProtoNetSearch...

Other

NextBio356668.
PROQ91W43.
SOURCESearch...

Entry information

Entry nameGCSP_MOUSE
AccessionPrimary (citable) accession number: Q91W43
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot