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Protein

Kinesin light chain 3

Gene

Klc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.

GO - Molecular functioni

  • kinesin binding Source: MGI
  • microtubule binding Source: MGI
  • microtubule motor activity Source: InterPro

GO - Biological processi

  • axo-dendritic transport Source: MGI
  • intraciliary transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin light chain 3
Gene namesi
Name:Klc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277971. Klc3.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

  • Note: Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.By similarity

GO - Cellular componenti

  • ciliary rootlet Source: MGI
  • cytoplasm Source: MGI
  • kinesin complex Source: InterPro
  • microtubule Source: UniProtKB-KW
  • motile cilium Source: MGI
  • neuron projection Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Kinesin light chain 3PRO_0000230786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731PhosphoserineCombined sources
Modified residuei467 – 4671PhosphoserineCombined sources
Modified residuei502 – 5021PhosphothreonineBy similarity
Modified residuei506 – 5061PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91W40.
MaxQBiQ91W40.
PaxDbiQ91W40.
PRIDEiQ91W40.

PTM databases

PhosphoSiteiQ91W40.

Expressioni

Tissue specificityi

Expressed in postmeiotic male germ cells (at protein level). Expressed in spleen, intestine, brain, ovary, testis, spermatocytes and spermatids.1 Publication

Gene expression databases

BgeeiQ91W40.
CleanExiMM_KLC3.
ExpressionAtlasiQ91W40. baseline and differential.
GenevisibleiQ91W40. MM.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Associates with microtubulin in an ATP-dependent manner. Interacts with KIF5C and ODF1 (By similarity).By similarity

GO - Molecular functioni

  • kinesin binding Source: MGI
  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi231327. 19 interactions.
DIPiDIP-48703N.
IntActiQ91W40. 17 interactions.
MINTiMINT-4121235.
STRINGi10090.ENSMUSP00000038091.

Structurei

3D structure databases

ProteinModelPortaliQ91W40.
SMRiQ91W40. Positions 197-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati207 – 24034TPR 1Add
BLAST
Repeati249 – 28234TPR 2Add
BLAST
Repeati291 – 32434TPR 3Add
BLAST
Repeati333 – 36634TPR 4Add
BLAST
Repeati375 – 40834TPR 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili88 – 15063Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi181 – 1844Poly-Glu
Compositional biasi190 – 1967Poly-Ala

Domaini

The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the kinesin light chain family.Curated
Contains 5 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiQ91W40.
KOiK10407.
OMAiCHPDVAT.
OrthoDBiEOG7GFB4R.
PhylomeDBiQ91W40.
TreeFamiTF314010.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91W40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVQVAAPGS TGLGPERLNP EELVRQTRQV VQGLEALRAE HHSLAGHLAE
60 70 80 90 100
ALAGPGPVAG VELLEEKQQV VNHSLEAIEL GLGEAQVLLA LSAHVSVLEA
110 120 130 140 150
EKQRLRAQAR RLAQENTWLR EELEETQRRL RASEEAVAQL EEEKSHLQFL
160 170 180 190 200
GQLRQYDPPE ESQRPESPPR RDSLASLFPS EEEEKKGPEA AGAAAAQQGG
210 220 230 240 250
YEIPARLRTL HNLVIQYAGQ GRYEVAVPLC RQALEDLERS SGHCHPDVAT
260 270 280 290 300
MLNILALVYR DQNKYKEATE LLHDALQIRE QTLGPEHPAV AATLNNLAVL
310 320 330 340 350
YGKRGRYREA EPLCQRALEI REKVLGADHP DVAKQLNNLA LLCQNQGKFQ
360 370 380 390 400
DVERHYARAL SIYEALGGPQ DPNVAKTKNN LASAYLKQNK YQQAEELYKE
410 420 430 440 450
ILSQEALPAP LGAPQGGTAG DTQQQVLRRS SSFSKLRESI RRGSEKLVSR
460 470 480 490 500
LRGEGMAGAA GMKRAMSLNM LNVDGPRAAR TQLSQLSTRH LSEAPRTLSI

STQDLSPR
Length:508
Mass (Da):56,006
Last modified:December 1, 2001 - v1
Checksum:i7F6F11CB943792C2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131L → P in BAE34354 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158094 mRNA. Translation: BAE34354.1.
BC017147 mRNA. Translation: AAH17147.1.
CCDSiCCDS20901.1.
RefSeqiNP_001272967.1. NM_001286038.1.
NP_001272968.1. NM_001286039.1.
NP_666294.1. NM_146182.4.
UniGeneiMm.213403.

Genome annotation databases

EnsembliENSMUST00000047170; ENSMUSP00000038091; ENSMUSG00000040714.
ENSMUST00000108458; ENSMUSP00000104098; ENSMUSG00000040714.
ENSMUST00000108459; ENSMUSP00000104099; ENSMUSG00000040714.
GeneIDi232943.
KEGGimmu:232943.
UCSCiuc009fls.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158094 mRNA. Translation: BAE34354.1.
BC017147 mRNA. Translation: AAH17147.1.
CCDSiCCDS20901.1.
RefSeqiNP_001272967.1. NM_001286038.1.
NP_001272968.1. NM_001286039.1.
NP_666294.1. NM_146182.4.
UniGeneiMm.213403.

3D structure databases

ProteinModelPortaliQ91W40.
SMRiQ91W40. Positions 197-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231327. 19 interactions.
DIPiDIP-48703N.
IntActiQ91W40. 17 interactions.
MINTiMINT-4121235.
STRINGi10090.ENSMUSP00000038091.

PTM databases

PhosphoSiteiQ91W40.

Proteomic databases

EPDiQ91W40.
MaxQBiQ91W40.
PaxDbiQ91W40.
PRIDEiQ91W40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047170; ENSMUSP00000038091; ENSMUSG00000040714.
ENSMUST00000108458; ENSMUSP00000104098; ENSMUSG00000040714.
ENSMUST00000108459; ENSMUSP00000104099; ENSMUSG00000040714.
GeneIDi232943.
KEGGimmu:232943.
UCSCiuc009fls.2. mouse.

Organism-specific databases

CTDi147700.
MGIiMGI:1277971. Klc3.

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiQ91W40.
KOiK10407.
OMAiCHPDVAT.
OrthoDBiEOG7GFB4R.
PhylomeDBiQ91W40.
TreeFamiTF314010.

Miscellaneous databases

NextBioi381353.
PROiQ91W40.
SOURCEiSearch...

Gene expression databases

BgeeiQ91W40.
CleanExiMM_KLC3.
ExpressionAtlasiQ91W40. baseline and differential.
GenevisibleiQ91W40. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Inner ear.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "Kinesin light-chain KLC3 expression in testis is restricted to spermatids."
    Junco A., Bhullar B., Tarnasky H.A., van der Hoorn F.A.
    Biol. Reprod. 64:1320-1330(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173 AND SER-467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiKLC3_MOUSE
AccessioniPrimary (citable) accession number: Q91W40
Secondary accession number(s): Q3TZ56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 1, 2001
Last modified: April 13, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.