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Q91W36

- UBP3_MOUSE

UniProt

Q91W36 - UBP3_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 3

Gene

Usp3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681NucleophilePROSITE-ProRule annotation
    Active sitei471 – 4711Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri27 – 10478UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA repair Source: Ensembl
    2. histone deubiquitination Source: Ensembl
    3. mitotic cell cycle Source: Ensembl
    4. regulation of protein stability Source: Ensembl
    5. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, DNA damage, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 3 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 3
    Ubiquitin thioesterase 3
    Ubiquitin-specific-processing protease 3
    Gene namesi
    Name:Usp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2152450. Usp3.

    Subcellular locationi

    Nucleus
    Note: Localizes preferentially with monoubiquitinated H2A to chromatin.

    GO - Cellular componenti

    1. nuclear chromatin Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Ubiquitin carboxyl-terminal hydrolase 3PRO_0000080620Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ91W36.
    PRIDEiQ91W36.

    PTM databases

    PhosphoSiteiQ91W36.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91W36.
    BgeeiQ91W36.
    CleanExiMM_USP3.
    GenevestigatoriQ91W36.

    Interactioni

    Subunit structurei

    Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.By similarity

    Protein-protein interaction databases

    IntActiQ91W36. 1 interaction.
    MINTiMINT-4139221.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91W36.
    SMRiQ91W36. Positions 3-508.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini159 – 511353USPAdd
    BLAST

    Domaini

    Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP3 subfamily.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri27 – 10478UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00750000117363.
    HOGENOMiHOG000231498.
    HOVERGENiHBG103589.
    InParanoidiQ91W36.
    KOiK11986.
    OMAiESCLYDL.
    OrthoDBiEOG7992Q2.
    PhylomeDBiQ91W36.
    TreeFamiTF315281.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91W36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG    50
    RYVNGHAKKH YEDAQIPLLN HKRSEKQEKA QHTVCMDCSS YSTYCYRCDD 100
    FVVNDTKLGL VQKVREHLQN LENSAFTADR HRKRKLLENS SLNSKLLKVN 150
    GSTTAICATG LRNLGNTCFM NAILQSLSNI EQFCCYFKEL PAVELRNGKT 200
    AGRRTYHTRS QGDSNVSLVE EFRKTLCALW QGSQTAFSPE SLFYVVWKIM 250
    PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK 300
    CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS 350
    KRSKNQENGP VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI 400
    QKLPKALCLH LKRFHWTAYL RNKVDTYVQF PLRGLDMKCY LLEPENSGPD 450
    SCLYDLAAVV VHHGSGVGSG HYTAYAVHEG RWFHFNDSTV TVTDEETVGK 500
    AKAYILFYVE RQARAGAEKL 520
    Length:520
    Mass (Da):58,868
    Last modified:December 1, 2001 - v1
    Checksum:iE160B48ECA47483D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK031141 mRNA. Translation: BAC27276.1.
    BC017156 mRNA. Translation: AAH17156.1.
    CCDSiCCDS23305.1.
    RefSeqiNP_659186.1. NM_144937.3.
    UniGeneiMm.38976.

    Genome annotation databases

    EnsembliENSMUST00000127569; ENSMUSP00000122199; ENSMUSG00000032376.
    GeneIDi235441.
    KEGGimmu:235441.
    UCSCiuc009qey.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK031141 mRNA. Translation: BAC27276.1 .
    BC017156 mRNA. Translation: AAH17156.1 .
    CCDSi CCDS23305.1.
    RefSeqi NP_659186.1. NM_144937.3.
    UniGenei Mm.38976.

    3D structure databases

    ProteinModelPortali Q91W36.
    SMRi Q91W36. Positions 3-508.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91W36. 1 interaction.
    MINTi MINT-4139221.

    Protein family/group databases

    MEROPSi C19.026.

    PTM databases

    PhosphoSitei Q91W36.

    Proteomic databases

    PaxDbi Q91W36.
    PRIDEi Q91W36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000127569 ; ENSMUSP00000122199 ; ENSMUSG00000032376 .
    GeneIDi 235441.
    KEGGi mmu:235441.
    UCSCi uc009qey.1. mouse.

    Organism-specific databases

    CTDi 9960.
    MGIi MGI:2152450. Usp3.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00750000117363.
    HOGENOMi HOG000231498.
    HOVERGENi HBG103589.
    InParanoidi Q91W36.
    KOi K11986.
    OMAi ESCLYDL.
    OrthoDBi EOG7992Q2.
    PhylomeDBi Q91W36.
    TreeFami TF315281.

    Miscellaneous databases

    NextBioi 382674.
    PROi Q91W36.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91W36.
    Bgeei Q91W36.
    CleanExi MM_USP3.
    Genevestigatori Q91W36.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Forelimb.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiUBP3_MOUSE
    AccessioniPrimary (citable) accession number: Q91W36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3