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Q91W36

- UBP3_MOUSE

UniProt

Q91W36 - UBP3_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 3
Gene
Usp3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Nucleophile By similarity
Active sitei471 – 4711Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 10478UBP-type
Add
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA repair Source: Ensembl
  2. histone deubiquitination Source: Ensembl
  3. mitotic cell cycle Source: Ensembl
  4. regulation of protein stability Source: Ensembl
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, DNA damage, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 3 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 3
Ubiquitin thioesterase 3
Ubiquitin-specific-processing protease 3
Gene namesi
Name:Usp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2152450. Usp3.

Subcellular locationi

Nucleus
Note: Localizes preferentially with monoubiquitinated H2A to chromatin.

GO - Cellular componenti

  1. nuclear chromatin Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Ubiquitin carboxyl-terminal hydrolase 3
PRO_0000080620Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ91W36.
PRIDEiQ91W36.

PTM databases

PhosphoSiteiQ91W36.

Expressioni

Gene expression databases

ArrayExpressiQ91W36.
BgeeiQ91W36.
CleanExiMM_USP3.
GenevestigatoriQ91W36.

Interactioni

Subunit structurei

Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A By similarity.

Protein-protein interaction databases

IntActiQ91W36. 1 interaction.
MINTiMINT-4139221.

Structurei

3D structure databases

ProteinModelPortaliQ91W36.
SMRiQ91W36. Positions 3-508.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini159 – 511353USP
Add
BLAST

Domaini

Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.

Sequence similaritiesi

Contains 1 USP domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00750000117363.
HOGENOMiHOG000231498.
HOVERGENiHBG103589.
InParanoidiQ91W36.
KOiK11986.
OMAiESCLYDL.
OrthoDBiEOG7992Q2.
PhylomeDBiQ91W36.
TreeFamiTF315281.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91W36-1 [UniParc]FASTAAdd to Basket

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MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG    50
RYVNGHAKKH YEDAQIPLLN HKRSEKQEKA QHTVCMDCSS YSTYCYRCDD 100
FVVNDTKLGL VQKVREHLQN LENSAFTADR HRKRKLLENS SLNSKLLKVN 150
GSTTAICATG LRNLGNTCFM NAILQSLSNI EQFCCYFKEL PAVELRNGKT 200
AGRRTYHTRS QGDSNVSLVE EFRKTLCALW QGSQTAFSPE SLFYVVWKIM 250
PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK 300
CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS 350
KRSKNQENGP VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI 400
QKLPKALCLH LKRFHWTAYL RNKVDTYVQF PLRGLDMKCY LLEPENSGPD 450
SCLYDLAAVV VHHGSGVGSG HYTAYAVHEG RWFHFNDSTV TVTDEETVGK 500
AKAYILFYVE RQARAGAEKL 520
Length:520
Mass (Da):58,868
Last modified:December 1, 2001 - v1
Checksum:iE160B48ECA47483D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK031141 mRNA. Translation: BAC27276.1.
BC017156 mRNA. Translation: AAH17156.1.
CCDSiCCDS23305.1.
RefSeqiNP_659186.1. NM_144937.3.
UniGeneiMm.38976.

Genome annotation databases

EnsembliENSMUST00000127569; ENSMUSP00000122199; ENSMUSG00000032376.
GeneIDi235441.
KEGGimmu:235441.
UCSCiuc009qey.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK031141 mRNA. Translation: BAC27276.1 .
BC017156 mRNA. Translation: AAH17156.1 .
CCDSi CCDS23305.1.
RefSeqi NP_659186.1. NM_144937.3.
UniGenei Mm.38976.

3D structure databases

ProteinModelPortali Q91W36.
SMRi Q91W36. Positions 3-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91W36. 1 interaction.
MINTi MINT-4139221.

Protein family/group databases

MEROPSi C19.026.

PTM databases

PhosphoSitei Q91W36.

Proteomic databases

PaxDbi Q91W36.
PRIDEi Q91W36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000127569 ; ENSMUSP00000122199 ; ENSMUSG00000032376 .
GeneIDi 235441.
KEGGi mmu:235441.
UCSCi uc009qey.1. mouse.

Organism-specific databases

CTDi 9960.
MGIi MGI:2152450. Usp3.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00750000117363.
HOGENOMi HOG000231498.
HOVERGENi HBG103589.
InParanoidi Q91W36.
KOi K11986.
OMAi ESCLYDL.
OrthoDBi EOG7992Q2.
PhylomeDBi Q91W36.
TreeFami TF315281.

Miscellaneous databases

NextBioi 382674.
PROi Q91W36.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q91W36.
Bgeei Q91W36.
CleanExi MM_USP3.
Genevestigatori Q91W36.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Forelimb.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Salivary gland.

Entry informationi

Entry nameiUBP3_MOUSE
AccessioniPrimary (citable) accession number: Q91W36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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