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Q91W36 (UBP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 3

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 3
Ubiquitin thioesterase 3
Ubiquitin-specific-processing protease 3
Gene names
Name:Usp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A By similarity.

Subcellular location

Nucleus. Note: Localizes preferentially with monoubiquitinated H2A to chromatin.

Domain

Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.

Sequence similarities

Belongs to the peptidase C19 family. USP3 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Ubiquitin carboxyl-terminal hydrolase 3
PRO_0000080620

Regions

Domain159 – 511353USP
Zinc finger27 – 10478UBP-type

Sites

Active site1681Nucleophile By similarity
Active site4711Proton acceptor By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q91W36 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: E160B48ECA47483D

FASTA52058,868
        10         20         30         40         50         60 
MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG RYVNGHAKKH 

        70         80         90        100        110        120 
YEDAQIPLLN HKRSEKQEKA QHTVCMDCSS YSTYCYRCDD FVVNDTKLGL VQKVREHLQN 

       130        140        150        160        170        180 
LENSAFTADR HRKRKLLENS SLNSKLLKVN GSTTAICATG LRNLGNTCFM NAILQSLSNI 

       190        200        210        220        230        240 
EQFCCYFKEL PAVELRNGKT AGRRTYHTRS QGDSNVSLVE EFRKTLCALW QGSQTAFSPE 

       250        260        270        280        290        300 
SLFYVVWKIM PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK 

       310        320        330        340        350        360 
CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS KRSKNQENGP 

       370        380        390        400        410        420 
VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI QKLPKALCLH LKRFHWTAYL 

       430        440        450        460        470        480 
RNKVDTYVQF PLRGLDMKCY LLEPENSGPD SCLYDLAAVV VHHGSGVGSG HYTAYAVHEG 

       490        500        510        520 
RWFHFNDSTV TVTDEETVGK AKAYILFYVE RQARAGAEKL 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Forelimb.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK031141 mRNA. Translation: BAC27276.1.
BC017156 mRNA. Translation: AAH17156.1.
RefSeqNP_659186.1. NM_144937.3.
UniGeneMm.38976.

3D structure databases

ProteinModelPortalQ91W36.
SMRQ91W36. Positions 1-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ91W36. 1 interaction.
MINTMINT-4139221.

Protein family/group databases

MEROPSC19.026.

PTM databases

PhosphoSiteQ91W36.

Proteomic databases

PaxDbQ91W36.
PRIDEQ91W36.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000127569; ENSMUSP00000122199; ENSMUSG00000032376.
GeneID235441.
KEGGmmu:235441.
UCSCuc009qey.1. mouse.

Organism-specific databases

CTD9960.
MGIMGI:2152450. Usp3.

Phylogenomic databases

eggNOGCOG5560.
GeneTreeENSGT00750000117363.
HOGENOMHOG000231498.
HOVERGENHBG103589.
InParanoidQ91W36.
KOK11986.
OMAESCLYDL.
OrthoDBEOG7992Q2.
PhylomeDBQ91W36.
TreeFamTF315281.

Gene expression databases

ArrayExpressQ91W36.
BgeeQ91W36.
CleanExMM_USP3.
GenevestigatorQ91W36.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio382674.
PROQ91W36.
SOURCESearch...

Entry information

Entry nameUBP3_MOUSE
AccessionPrimary (citable) accession number: Q91W36
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot