ID COX42_MOUSE Reviewed; 172 AA. AC Q91W29; DT 03-APR-2002, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 27-MAR-2024, entry version 144. DE RecName: Full=Cytochrome c oxidase subunit 4 isoform 2, mitochondrial; DE AltName: Full=Cytochrome c oxidase subunit IV isoform 2; DE Short=COX IV-2; DE Flags: Precursor; GN Name=Cox4i2; Synonyms=Cox4b; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Heart; RX PubMed=11311561; DOI=10.1016/s0378-1119(01)00385-7; RA Huettemann M., Kadenbach B., Grossman L.I.; RT "Mammalian subunit IV isoforms of cytochrome c oxidase."; RL Gene 267:111-123(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Lung; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the CC mitochondrial electron transport chain which drives oxidative CC phosphorylation. The respiratory chain contains 3 multisubunit CC complexes succinate dehydrogenase (complex II, CII), ubiquinol- CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to CC transfer electrons derived from NADH and succinate to molecular oxygen, CC creating an electrochemical gradient over the inner membrane that CC drives transmembrane transport and the ATP synthase. Cytochrome c CC oxidase is the component of the respiratory chain that catalyzes the CC reduction of oxygen to water. Electrons originating from reduced CC cytochrome c in the intermembrane space (IMS) are transferred via the CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 CC to the active site in subunit 1, a binuclear center (BNC) formed by CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 CC water molecules using 4 electrons from cytochrome c in the IMS and 4 CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P00424}. CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation. CC {ECO:0000250|UniProtKB:P00424}. CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a CC multisubunit enzyme composed of 14 subunits. The complex is composed of CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in CC the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, CC COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which CC are encoded in the nuclear genome. The complex exists as a monomer or a CC dimer and forms supercomplexes (SCs) in the inner mitochondrial CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex CC III, CIII), resulting in different assemblies (supercomplex CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). CC {ECO:0000250|UniProtKB:P00423}. CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane CC {ECO:0000250|UniProtKB:P00423}; Single-pass membrane protein CC {ECO:0000250|UniProtKB:P00423}. CC -!- SIMILARITY: Belongs to the cytochrome c oxidase IV family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF271382; AAK49433.1; -; mRNA. DR EMBL; BC049623; AAH49623.1; -; mRNA. DR CCDS; CCDS16898.1; -. DR RefSeq; NP_444321.1; NM_053091.2. DR RefSeq; XP_017174836.1; XM_017319347.1. DR AlphaFoldDB; Q91W29; -. DR SMR; Q91W29; -. DR STRING; 10090.ENSMUSP00000105446; -. DR PhosphoSitePlus; Q91W29; -. DR MaxQB; Q91W29; -. DR PaxDb; 10090-ENSMUSP00000105446; -. DR ProteomicsDB; 284103; -. DR Antibodypedia; 25206; 211 antibodies from 30 providers. DR DNASU; 84682; -. DR Ensembl; ENSMUST00000010020.12; ENSMUSP00000010020.6; ENSMUSG00000009876.14. DR Ensembl; ENSMUST00000109821.2; ENSMUSP00000105446.2; ENSMUSG00000009876.14. DR GeneID; 84682; -. DR KEGG; mmu:84682; -. DR UCSC; uc008ngg.1; mouse. DR AGR; MGI:2135755; -. DR CTD; 84701; -. DR MGI; MGI:2135755; Cox4i2. DR VEuPathDB; HostDB:ENSMUSG00000009876; -. DR eggNOG; KOG4075; Eukaryota. DR GeneTree; ENSGT00390000002407; -. DR HOGENOM; CLU_117340_1_1_1; -. DR InParanoid; Q91W29; -. DR OMA; LSDEWKH; -. DR OrthoDB; 5343704at2759; -. DR PhylomeDB; Q91W29; -. DR TreeFam; TF105061; -. DR BRENDA; 7.1.1.9; 3474. DR UniPathway; UPA00705; -. DR BioGRID-ORCS; 84682; 3 hits in 79 CRISPR screens. DR ChiTaRS; Cox4i2; mouse. DR PRO; PR:Q91W29; -. DR Proteomes; UP000000589; Chromosome 2. DR RNAct; Q91W29; Protein. DR Bgee; ENSMUSG00000009876; Expressed in retinal neural layer and 107 other cell types or tissues. DR GO; GO:0031966; C:mitochondrial membrane; IDA:MGI. DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; ISA:MGI. DR GO; GO:0005739; C:mitochondrion; HDA:MGI. DR GO; GO:0004129; F:cytochrome-c oxidase activity; ISA:MGI. DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; ISO:MGI. DR CDD; cd00922; Cyt_c_Oxidase_IV; 1. DR Gene3D; 1.10.442.10; Cytochrome c oxidase subunit IV; 1. DR InterPro; IPR013288; Cyt_c_oxidase_su4. DR InterPro; IPR004203; Cyt_c_oxidase_su4_fam. DR InterPro; IPR036639; Cyt_c_oxidase_su4_sf. DR PANTHER; PTHR10707:SF11; CYTOCHROME C OXIDASE SUBUNIT 4 ISOFORM 2, MITOCHONDRIAL; 1. DR PANTHER; PTHR10707; CYTOCHROME C OXIDASE SUBUNIT IV; 1. DR Pfam; PF02936; COX4; 1. DR PRINTS; PR01873; CYTCOXIDASE4. DR SUPFAM; SSF81406; Mitochondrial cytochrome c oxidase subunit IV; 1. DR Genevisible; Q91W29; MM. PE 1: Evidence at protein level; KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; KW Transit peptide; Transmembrane; Transmembrane helix. FT TRANSIT 1..34 FT /note="Mitochondrion" FT /evidence="ECO:0000255" FT CHAIN 35..172 FT /note="Cytochrome c oxidase subunit 4 isoform 2, FT mitochondrial" FT /id="PRO_0000006090" FT TOPO_DOM 35..101 FT /note="Mitochondrial matrix" FT /evidence="ECO:0000250|UniProtKB:P00423" FT TRANSMEM 102..127 FT /note="Helical" FT /evidence="ECO:0000250|UniProtKB:P00423" FT TOPO_DOM 128..172 FT /note="Mitochondrial intermembrane" FT /evidence="ECO:0000250|UniProtKB:P00423" FT REGION 13..32 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 15..29 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 172 AA; 20206 MW; A2395EF4FD68CEA6 CRC64; MFSRAARSLV MRTGLRTRGT GTHSPGDAAG SQRRMTPYVD CYAQRSYPMP DEPFCTELSE EQRALKEKEK GSWTQLSQAE KVALYRLQFH ETFAEMNHRS NEWKTVMGCV FFFIGFTALV IWWQRVYVFP KKVVTLTEER KAQQLQRLLD MKSNPIQGLA AHWDYEKKEW KK //