Q91W18 (TDRD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tudor domain-containing protein 3

Gene names

Name:

Tdrd3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins By similarity.
Subunit structure	Component of mRNA stress granules. Interacts with FMR1, FXR1, FXR2, EWSR1, FUS, SERBP1, EEF1A1 and DDX3X or DDX3Y, and with the small nuclear ribonucleoprotein-associated proteins SNRPB and SNRPN. Interacts with 'Lys-48'-linked tetra-ubiquitin, but not with monoubiquitin or 'Lys-63'-linked ubiquitin chains By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly cytoplasmic. Associated with actively translating polyribosomes and with mRNA stress granules By similarity.
Domain	The Tudor domain specifically recognizes and binds asymmetric dimethylation of histone H3 'Arg-17' (H3R17me2a) and histones H4 'Arg-3', 2 tags for epigenetic transcriptional activation By similarity.
Sequence similarities	Contains 1 Tudor domain. Contains 1 UBA domain.
Sequence caution	The sequence AAH05670.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH66144.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC26033.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC28263.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB methylated histone residue binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91W18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91W18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     706-743: EEEGTYDHTIEFRRGGDGQPRRSTRPTQQFYQPPRARN → VRDPNSVQRLL

Note: No experimental confirmation available.

Isoform 3 (identifier: Q91W18-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MAEVSGAALSQAGW → MLFILFNR

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 743

743

Tudor domain-containing protein 3

PRO_0000183164

Regions

Domain

286 – 326

41

UBA

Domain

647 – 707

61

Tudor

Amino acid modifications

Modified residue

349

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 14

14

MAEVS…SQAGW → MLFILFNR in isoform 3.

VSP_037054

Alternative sequence

706 – 743

38

EEEGT…PRARN → VRDPNSVQRLL in isoform 2.

VSP_034485

Experimental info

Sequence conflict

8

1

A → G in BAC28263. Ref.1

Sequence conflict

57

1

I → V in AAH66144. Ref.3

Sequence conflict

350

1

E → V in BAC28263. Ref.1

Sequence conflict

365

1

T → M in AAH66144. Ref.3

Sequence conflict

375

1

G → R in AAH66144. Ref.3

Sequence conflict

408

1

T → S in AAH05670. Ref.3

Sequence conflict

469

1

K → R in AAH05670. Ref.3

Sequence conflict

489

1

L → F in AAH05670. Ref.3

Sequence conflict

559

1

V → F in AAH05670. Ref.3

Sequence conflict

576

1

R → S in AAH05670. Ref.3

Sequence conflict

679

1

A → E in BAC28263. Ref.1

Sequence conflict

717

1

F → L in AAH66144. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

743

Helix Strand Turn

Details...

Beta strand

653 – 657

5

Turn

659 – 661

3

Beta strand

664 – 672

9

Beta strand

678 – 683

6

Beta strand

689 – 693

5

Helix

694 – 696

3

Beta strand

697 – 699

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: 23792E13FFE3A4B6	FASTA	743	82,253
	10 20 30 40 50 60 MAEVSGAALS QAGWYLSDEG VEACTSSPGK GSINDIILIA LNTDLRTIGK KFLPSDINGG 70 80 90 100 110 120 KVEKLEGPCV LQIQKVRNVA APKDNEESQA APRMLRVQMT DGHTSCTAVE FSYISKISLN 130 140 150 160 170 180 TPPGTKVKLS GTVDIKNGFL LLSDSNTTVL GGEVEHLIDK WALQRSLLKH NRSNIGAEGG 190 200 210 220 230 240 PPPFLPFGQK CASNVQVDSR ELDRRKTLQV SLPAKPANDN DEFEKQRTAA IAEVAKSKET 250 260 270 280 290 300 KTFGGGGGGA RSNLNIGAAG HRNREVLQKE KASKSESKNE GVYRELVDEK ALKHITEMGF 310 320 330 340 350 360 SKEASRQALM DNANNLEAAL NVLLNSSKQK PAVGPPARGR GKGRGRGRSE DEEDLGTARP 370 380 390 400 410 420 SAPSTLFDFL ESKMGTLNVE EPKSQPQHLH QGQHRGWNAE QNGMKDGTQS RHLPRNDTRQ 430 440 450 460 470 480 PRNERPPRFQ KDTPTSKSTV ENSVLSRNRG SERPSSSSGS DVWAEERIKC DRPYSRYDRT 490 500 510 520 530 540 KDASHPLGLQ HNDGAFKKRE NSMQNRPGRG PLYAEAKENP LPPEFVDYNN QRRGRRENQT 550 560 570 580 590 600 GHPDHCYERK PRTMNSEAVS GLKIEKHFSV NTDYPRPVQS NSLGVPNGET APPLKGRRVG 610 620 630 640 650 660 PIKSAGPVTA VPYDDKIFYN SGPKRRSGPI KPEKVIESSI PVEYAKVWKP GDECFALYWE 670 680 690 700 710 720 DNKFYRAEVE ALHSSGMTAV VKFTDYGNYE EVLLSNIKPV QTEAWEEEGT YDHTIEFRRG 730 740 GDGQPRRSTR PTQQFYQPPR ARN
	Isoform 2 [UniParc]. Checksum: A877E5669CF4779A	FASTA	716	79,014
	10 20 30 40 50 60 MAEVSGAALS QAGWYLSDEG VEACTSSPGK GSINDIILIA LNTDLRTIGK KFLPSDINGG 70 80 90 100 110 120 KVEKLEGPCV LQIQKVRNVA APKDNEESQA APRMLRVQMT DGHTSCTAVE FSYISKISLN 130 140 150 160 170 180 TPPGTKVKLS GTVDIKNGFL LLSDSNTTVL GGEVEHLIDK WALQRSLLKH NRSNIGAEGG 190 200 210 220 230 240 PPPFLPFGQK CASNVQVDSR ELDRRKTLQV SLPAKPANDN DEFEKQRTAA IAEVAKSKET 250 260 270 280 290 300 KTFGGGGGGA RSNLNIGAAG HRNREVLQKE KASKSESKNE GVYRELVDEK ALKHITEMGF 310 320 330 340 350 360 SKEASRQALM DNANNLEAAL NVLLNSSKQK PAVGPPARGR GKGRGRGRSE DEEDLGTARP 370 380 390 400 410 420 SAPSTLFDFL ESKMGTLNVE EPKSQPQHLH QGQHRGWNAE QNGMKDGTQS RHLPRNDTRQ 430 440 450 460 470 480 PRNERPPRFQ KDTPTSKSTV ENSVLSRNRG SERPSSSSGS DVWAEERIKC DRPYSRYDRT 490 500 510 520 530 540 KDASHPLGLQ HNDGAFKKRE NSMQNRPGRG PLYAEAKENP LPPEFVDYNN QRRGRRENQT 550 560 570 580 590 600 GHPDHCYERK PRTMNSEAVS GLKIEKHFSV NTDYPRPVQS NSLGVPNGET APPLKGRRVG 610 620 630 640 650 660 PIKSAGPVTA VPYDDKIFYN SGPKRRSGPI KPEKVIESSI PVEYAKVWKP GDECFALYWE 670 680 690 700 710 DNKFYRAEVE ALHSSGMTAV VKFTDYGNYE EVLLSNIKPV QTEAWVRDPN SVQRLL
	Isoform 3 [UniParc]. Checksum: 3FFEE2763D5BE42F	FASTA	737	81,929
	10 20 30 40 50 60 MLFILFNRYL SDEGVEACTS SPGKGSINDI ILIALNTDLR TIGKKFLPSD INGGKVEKLE 70 80 90 100 110 120 GPCVLQIQKV RNVAAPKDNE ESQAAPRMLR VQMTDGHTSC TAVEFSYISK ISLNTPPGTK 130 140 150 160 170 180 VKLSGTVDIK NGFLLLSDSN TTVLGGEVEH LIDKWALQRS LLKHNRSNIG AEGGPPPFLP 190 200 210 220 230 240 FGQKCASNVQ VDSRELDRRK TLQVSLPAKP ANDNDEFEKQ RTAAIAEVAK SKETKTFGGG 250 260 270 280 290 300 GGGARSNLNI GAAGHRNREV LQKEKASKSE SKNEGVYREL VDEKALKHIT EMGFSKEASR 310 320 330 340 350 360 QALMDNANNL EAALNVLLNS SKQKPAVGPP ARGRGKGRGR GRSEDEEDLG TARPSAPSTL 370 380 390 400 410 420 FDFLESKMGT LNVEEPKSQP QHLHQGQHRG WNAEQNGMKD GTQSRHLPRN DTRQPRNERP 430 440 450 460 470 480 PRFQKDTPTS KSTVENSVLS RNRGSERPSS SSGSDVWAEE RIKCDRPYSR YDRTKDASHP 490 500 510 520 530 540 LGLQHNDGAF KKRENSMQNR PGRGPLYAEA KENPLPPEFV DYNNQRRGRR ENQTGHPDHC 550 560 570 580 590 600 YERKPRTMNS EAVSGLKIEK HFSVNTDYPR PVQSNSLGVP NGETAPPLKG RRVGPIKSAG 610 620 630 640 650 660 PVTAVPYDDK IFYNSGPKRR SGPIKPEKVI ESSIPVEYAK VWKPGDECFA LYWEDNKFYR 670 680 690 700 710 720 AEVEALHSSG MTAVVKFTDY GNYEEVLLSN IKPVQTEAWE EEGTYDHTIE FRRGGDGQPR 730 RSTRPTQQFY QPPRARN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 545-743 (ISOFORM 2). Strain: C57BL/6J. Tissue: Lung and Skin.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Embryo and Mammary tumor.
[4]	"Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 646-699.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK028617 mRNA. Translation: BAC26033.1. Different initiation.
AK033390 mRNA. Translation: BAC28263.1. Different initiation.
AC154562 Genomic DNA. No translation available.
CT010453 Genomic DNA. No translation available.
BC005670 mRNA. Translation: AAH05670.3. Different initiation.
BC066144 mRNA. Translation: AAH66144.1. Different initiation.

IPI

IPI00227152.
IPI00896611.
IPI01008137.

RefSeq

NP_766193.3. NM_172605.3.

UniGene

Mm.35168.
Mm.474717.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D9T	NMR	-	A	646-705	[»]

ProteinModelPortal

Q91W18.

SMR

Q91W18. Positions 2-164, 287-337, 645-705.

ModBase

Search...

PTM databases

PhosphoSite

Q91W18.

Proteomic databases

PRIDE

Q91W18.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000168275; ENSMUSP00000129019; ENSMUSG00000022019.
ENSMUST00000169504; ENSMUSP00000131542; ENSMUSG00000022019.
ENSMUST00000170865; ENSMUSP00000126189; ENSMUSG00000022019.

GeneID

219249.

KEGG

mmu:219249.

UCSC

uc007uuf.2. mouse.
uc007uug.2. mouse.
uc011zpb.1. mouse.

Organism-specific databases

CTD

81550.

MGI

MGI:2444023. Tdrd3.

Phylogenomic databases

eggNOG

roNOG11489.

GeneTree

ENSGT00440000038925.

HOGENOM

HBG402882.

HOVERGEN

HBG059153.

InParanoid

Q91W18.

OrthoDB

EOG4WM4T9.

Gene expression databases

ArrayExpress

Q91W18.

Bgee

Q91W18.

CleanEx

MM_TDRD3.

Genevestigator

Q91W18.

Family and domain databases

InterPro

IPR013894. DUF1767.
IPR010304. Survival_motor_neuron.
IPR002999. Tudor.
IPR018351. Tudor_subgr.
IPR009060. UBA-like.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]

Pfam

PF08585. DUF1767. 1 hit.
PF06003. SMN. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]

SMART

SM00333. TUDOR. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]

SUPFAM

SSF46934. UBA_like. 1 hit.

PROSITE

PS50304. TUDOR. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

376651.

SOURCE

Search...

Entry information

Entry name

TDRD3_MOUSE

Accession

Primary (citable) accession number: Q91W18
Secondary accession number(s): E9Q2Q4 , Q6NZG7, Q8BZW6, Q8C1A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	July 27, 2011
Last modified:	November 16, 2011
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program