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Protein

Tudor domain-containing protein 3

Gene

Tdrd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Names & Taxonomyi

Protein namesi
Recommended name:
Tudor domain-containing protein 3
Gene namesi
Name:Tdrd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2444023. Tdrd3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Associated with actively translating polyribosomes and with mRNA stress granules (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 743743Tudor domain-containing protein 3PRO_0000183164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei349 – 3491PhosphoserineCombined sources
Modified residuei438 – 4381PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91W18.
PaxDbiQ91W18.
PeptideAtlasiQ91W18.
PRIDEiQ91W18.

PTM databases

iPTMnetiQ91W18.
PhosphoSiteiQ91W18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022019.
CleanExiMM_TDRD3.
ExpressionAtlasiQ91W18. baseline and differential.
GenevisibleiQ91W18. MM.

Interactioni

Subunit structurei

Component of mRNA stress granules. Interacts with FMR1, FXR1, FXR2, EWSR1, FUS, SERBP1, EEF1A1 and DDX3X or DDX3Y, and with the small nuclear ribonucleoprotein-associated proteins SNRPB and SNRPN. Interacts with 'Lys-48'-linked tetra-ubiquitin, but not with monoubiquitin or 'Lys-63'-linked ubiquitin chains. May interact with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A. Interacts with POLR2A (via the C-terminal domain (CTD)).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230129. 1 interaction.
IntActiQ91W18. 1 interaction.
MINTiMINT-4137349.
STRINGi10090.ENSMUSP00000129019.

Structurei

Secondary structure

1
743
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi653 – 6575Combined sources
Turni659 – 6613Combined sources
Beta strandi664 – 6729Combined sources
Beta strandi674 – 68310Combined sources
Turni684 – 6863Combined sources
Beta strandi689 – 6935Combined sources
Helixi694 – 6963Combined sources
Beta strandi697 – 6993Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9TNMR-A646-705[»]
ProteinModelPortaliQ91W18.
SMRiQ91W18. Positions 41-162, 287-337, 645-705.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91W18.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 32641UBAPROSITE-ProRule annotationAdd
BLAST
Domaini647 – 70761TudorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni723 – 74321EBM motif; may mediate interaction with the EJCBy similarityAdd
BLAST

Domaini

The Tudor domain specifically recognizes and binds asymmetric dimethylation of histone H3 'Arg-17' (H3R17me2a) and histones H4 'Arg-3', 2 tags for epigenetic transcriptional activation.By similarity

Sequence similaritiesi

Contains 1 Tudor domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3683. Eukaryota.
ENOG4111KPK. LUCA.
GeneTreeiENSGT00740000115530.
HOGENOMiHOG000154539.
HOVERGENiHBG059153.
InParanoidiQ91W18.
KOiK18404.
OMAiVNDIILI.
OrthoDBiEOG091G0GU8.
TreeFamiTF316491.

Family and domain databases

InterProiIPR033472. DUF1767.
IPR013894. RMI1_N.
IPR010304. Survival_motor_neuron.
IPR002999. Tudor.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF08585. RMI1_N. 1 hit.
PF06003. SMN. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM01161. DUF1767. 1 hit.
SM00333. TUDOR. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50304. TUDOR. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91W18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVSGAALS QAGWYLSDEG VEACTSSPGK GSINDIILIA LNTDLRTIGK
60 70 80 90 100
KFLPSDINGG KVEKLEGPCV LQIQKVRNVA APKDNEESQA APRMLRVQMT
110 120 130 140 150
DGHTSCTAVE FSYISKISLN TPPGTKVKLS GTVDIKNGFL LLSDSNTTVL
160 170 180 190 200
GGEVEHLIDK WALQRSLLKH NRSNIGAEGG PPPFLPFGQK CASNVQVDSR
210 220 230 240 250
ELDRRKTLQV SLPAKPANDN DEFEKQRTAA IAEVAKSKET KTFGGGGGGA
260 270 280 290 300
RSNLNIGAAG HRNREVLQKE KASKSESKNE GVYRELVDEK ALKHITEMGF
310 320 330 340 350
SKEASRQALM DNANNLEAAL NVLLNSSKQK PAVGPPARGR GKGRGRGRSE
360 370 380 390 400
DEEDLGTARP SAPSTLFDFL ESKMGTLNVE EPKSQPQHLH QGQHRGWNAE
410 420 430 440 450
QNGMKDGTQS RHLPRNDTRQ PRNERPPRFQ KDTPTSKSTV ENSVLSRNRG
460 470 480 490 500
SERPSSSSGS DVWAEERIKC DRPYSRYDRT KDASHPLGLQ HNDGAFKKRE
510 520 530 540 550
NSMQNRPGRG PLYAEAKENP LPPEFVDYNN QRRGRRENQT GHPDHCYERK
560 570 580 590 600
PRTMNSEAVS GLKIEKHFSV NTDYPRPVQS NSLGVPNGET APPLKGRRVG
610 620 630 640 650
PIKSAGPVTA VPYDDKIFYN SGPKRRSGPI KPEKVIESSI PVEYAKVWKP
660 670 680 690 700
GDECFALYWE DNKFYRAEVE ALHSSGMTAV VKFTDYGNYE EVLLSNIKPV
710 720 730 740
QTEAWEEEGT YDHTIEFRRG GDGQPRRSTR PTQQFYQPPR ARN
Length:743
Mass (Da):82,253
Last modified:July 27, 2011 - v4
Checksum:i23792E13FFE3A4B6
GO
Isoform 2 (identifier: Q91W18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     706-743: EEEGTYDHTIEFRRGGDGQPRRSTRPTQQFYQPPRARN → VRDPNSVQRLL

Note: No experimental confirmation available.
Show »
Length:716
Mass (Da):79,014
Checksum:iA877E5669CF4779A
GO
Isoform 3 (identifier: Q91W18-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MAEVSGAALSQAGW → MLFILFNR

Note: No experimental confirmation available.
Show »
Length:737
Mass (Da):81,929
Checksum:i3FFEE2763D5BE42F
GO

Sequence cautioni

The sequence AAH05670 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH66144 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC26033 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC28263 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81A → G in BAC28263 (PubMed:16141072).Curated
Sequence conflicti57 – 571I → V in AAH66144 (PubMed:15489334).Curated
Sequence conflicti350 – 3501E → V in BAC28263 (PubMed:16141072).Curated
Sequence conflicti365 – 3651T → M in AAH66144 (PubMed:15489334).Curated
Sequence conflicti375 – 3751G → R in AAH66144 (PubMed:15489334).Curated
Sequence conflicti408 – 4081T → S in AAH05670 (PubMed:15489334).Curated
Sequence conflicti469 – 4691K → R in AAH05670 (PubMed:15489334).Curated
Sequence conflicti489 – 4891L → F in AAH05670 (PubMed:15489334).Curated
Sequence conflicti559 – 5591V → F in AAH05670 (PubMed:15489334).Curated
Sequence conflicti576 – 5761R → S in AAH05670 (PubMed:15489334).Curated
Sequence conflicti679 – 6791A → E in BAC28263 (PubMed:16141072).Curated
Sequence conflicti717 – 7171F → L in AAH66144 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1414MAEVS…SQAGW → MLFILFNR in isoform 3. 1 PublicationVSP_037054Add
BLAST
Alternative sequencei706 – 74338EEEGT…PRARN → VRDPNSVQRLL in isoform 2. 1 PublicationVSP_034485Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028617 mRNA. Translation: BAC26033.1. Different initiation.
AK033390 mRNA. Translation: BAC28263.1. Different initiation.
AC154562 Genomic DNA. No translation available.
CT010453 Genomic DNA. No translation available.
BC005670 mRNA. Translation: AAH05670.3. Different initiation.
BC066144 mRNA. Translation: AAH66144.1. Different initiation.
CCDSiCCDS27305.2. [Q91W18-1]
CCDS56972.1. [Q91W18-2]
RefSeqiNP_001240684.1. NM_001253755.1. [Q91W18-2]
NP_766193.3. NM_172605.3. [Q91W18-1]
UniGeneiMm.35168.

Genome annotation databases

EnsembliENSMUST00000168275; ENSMUSP00000129019; ENSMUSG00000022019. [Q91W18-1]
ENSMUST00000169504; ENSMUSP00000131542; ENSMUSG00000022019. [Q91W18-2]
ENSMUST00000170865; ENSMUSP00000126189; ENSMUSG00000022019. [Q91W18-3]
GeneIDi219249.
KEGGimmu:219249.
UCSCiuc007uuf.3. mouse. [Q91W18-2]
uc007uug.2. mouse. [Q91W18-1]
uc011zpb.1. mouse. [Q91W18-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028617 mRNA. Translation: BAC26033.1. Different initiation.
AK033390 mRNA. Translation: BAC28263.1. Different initiation.
AC154562 Genomic DNA. No translation available.
CT010453 Genomic DNA. No translation available.
BC005670 mRNA. Translation: AAH05670.3. Different initiation.
BC066144 mRNA. Translation: AAH66144.1. Different initiation.
CCDSiCCDS27305.2. [Q91W18-1]
CCDS56972.1. [Q91W18-2]
RefSeqiNP_001240684.1. NM_001253755.1. [Q91W18-2]
NP_766193.3. NM_172605.3. [Q91W18-1]
UniGeneiMm.35168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9TNMR-A646-705[»]
ProteinModelPortaliQ91W18.
SMRiQ91W18. Positions 41-162, 287-337, 645-705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230129. 1 interaction.
IntActiQ91W18. 1 interaction.
MINTiMINT-4137349.
STRINGi10090.ENSMUSP00000129019.

PTM databases

iPTMnetiQ91W18.
PhosphoSiteiQ91W18.

Proteomic databases

MaxQBiQ91W18.
PaxDbiQ91W18.
PeptideAtlasiQ91W18.
PRIDEiQ91W18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000168275; ENSMUSP00000129019; ENSMUSG00000022019. [Q91W18-1]
ENSMUST00000169504; ENSMUSP00000131542; ENSMUSG00000022019. [Q91W18-2]
ENSMUST00000170865; ENSMUSP00000126189; ENSMUSG00000022019. [Q91W18-3]
GeneIDi219249.
KEGGimmu:219249.
UCSCiuc007uuf.3. mouse. [Q91W18-2]
uc007uug.2. mouse. [Q91W18-1]
uc011zpb.1. mouse. [Q91W18-3]

Organism-specific databases

CTDi81550.
MGIiMGI:2444023. Tdrd3.

Phylogenomic databases

eggNOGiKOG3683. Eukaryota.
ENOG4111KPK. LUCA.
GeneTreeiENSGT00740000115530.
HOGENOMiHOG000154539.
HOVERGENiHBG059153.
InParanoidiQ91W18.
KOiK18404.
OMAiVNDIILI.
OrthoDBiEOG091G0GU8.
TreeFamiTF316491.

Miscellaneous databases

EvolutionaryTraceiQ91W18.
PROiQ91W18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022019.
CleanExiMM_TDRD3.
ExpressionAtlasiQ91W18. baseline and differential.
GenevisibleiQ91W18. MM.

Family and domain databases

InterProiIPR033472. DUF1767.
IPR013894. RMI1_N.
IPR010304. Survival_motor_neuron.
IPR002999. Tudor.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF08585. RMI1_N. 1 hit.
PF06003. SMN. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM01161. DUF1767. 1 hit.
SM00333. TUDOR. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50304. TUDOR. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTDRD3_MOUSE
AccessioniPrimary (citable) accession number: Q91W18
Secondary accession number(s): E9Q2Q4
, Q6NZG7, Q8BZW6, Q8C1A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.