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Protein

Stromal membrane-associated protein 1

Gene

Smap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activating protein that acts on ARF6. Plays a role in clathrin-dependent endocytosis. May play a role in erythropoiesis.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 5624C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • clathrin binding Source: MGI
  • GTPase activator activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of erythrocyte differentiation Source: MGI
  • regulation of clathrin-mediated endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal membrane-associated protein 1
Gene namesi
Name:Smap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2138261. Smap1.

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytoplasm Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi61 – 611R → Q: Loss of GTPase activation. 1 Publication
Mutagenesisi192 – 1965LLGLD → AAAAA: Loss of interaction with clathrin heavy chains. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Stromal membrane-associated protein 1PRO_0000235839Add
BLAST

Proteomic databases

EPDiQ91VZ6.
MaxQBiQ91VZ6.
PaxDbiQ91VZ6.
PeptideAtlasiQ91VZ6.
PRIDEiQ91VZ6.

PTM databases

iPTMnetiQ91VZ6.
PhosphoSiteiQ91VZ6.

Expressioni

Tissue specificityi

Detected in adult brain, lung, heart, liver, ovary and bone marrow. Detected in stromal cells of the red pulp of adult spleen.1 Publication

Developmental stagei

Detected in stromal cells of fetal liver at 10.5 days. Expression was maximal after 14 days of development and decreased thereafter. Detected at low levels after 18 days.1 Publication

Gene expression databases

BgeeiQ91VZ6.
CleanExiMM_SMAP1.
ExpressionAtlasiQ91VZ6. baseline and differential.
GenevisibleiQ91VZ6. MM.

Interactioni

Subunit structurei

Interacts with ARF6. Interacts with clathrin heavy chains via the clathrin box-like motif.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Smap2Q7TN294EBI-8317690,EBI-11358641

GO - Molecular functioni

  • clathrin binding Source: MGI

Protein-protein interaction databases

BioGridi221043. 11 interactions.
IntActiQ91VZ6. 14 interactions.
MINTiMINT-4121188.
STRINGi10090.ENSMUSP00000027339.

Structurei

3D structure databases

ProteinModelPortaliQ91VZ6.
SMRiQ91VZ6. Positions 9-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 143126Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi192 – 1965Interaction with clathrin heavy chains

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 5624C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00820000127006.
HOGENOMiHOG000230583.
HOVERGENiHBG055260.
InParanoidiQ91VZ6.
KOiK12486.
OMAiGMPNGFT.
OrthoDBiEOG78M02F.
PhylomeDBiQ91VZ6.
TreeFamiTF313876.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV
60 70 80 90 100
FICIRCAGIH RNLGVHISRV KSVNLDQWTP EQIQCMQDMG NTKARLLYEA
110 120 130 140 150
NLPENFRRPQ TDQAVEFFIR DKYEKKKYYD KNAIAITNKE KEKKKDEKKR
160 170 180 190 200
EKEPEKPAKP LTTEKLPKKE EQQLEPKKST SPKNAAEPTI DLLGLDGPAE
210 220 230 240 250
APVTNGNPAT APALSDDLDI FGPMISNPLP AAVMPPAQGT ASVPAPATLS
260 270 280 290 300
TVTSGDLDLF TEQTTKSEEV AKKQLSKDSI LSLYGTGAQQ STPGVFMGPT
310 320 330 340 350
NIPFTSQAPT AFQGFPSMGV PVPAAPGLIG NMMGQNTGMM VGMPMHNGFM
360 370 380 390 400
GNAQTGVMPL PQNVVGPQGG MVGQMGAPQS KFGLPQAQQP QWNLSQMNQQ
410 420 430 440
MAAMNLSSAN ASAGFGQPPS TTAGWSGSSS GQTLSTQLWK
Length:440
Mass (Da):47,660
Last modified:December 1, 2001 - v1
Checksum:iE1852548BAFBC229
GO

Sequence cautioni

The sequence AAI00538.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006946 mRNA. Translation: AAH06946.1.
BC080286 mRNA. Translation: AAH80286.1.
BC100537 mRNA. Translation: AAI00538.1. Sequence problems.
CCDSiCCDS14851.1.
RefSeqiNP_001277612.1. NM_001290683.1.
NP_082810.1. NM_028534.3.
UniGeneiMm.329963.

Genome annotation databases

EnsembliENSMUST00000027339; ENSMUSP00000027339; ENSMUSG00000026155.
GeneIDi98366.
KEGGimmu:98366.
UCSCiuc007amg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006946 mRNA. Translation: AAH06946.1.
BC080286 mRNA. Translation: AAH80286.1.
BC100537 mRNA. Translation: AAI00538.1. Sequence problems.
CCDSiCCDS14851.1.
RefSeqiNP_001277612.1. NM_001290683.1.
NP_082810.1. NM_028534.3.
UniGeneiMm.329963.

3D structure databases

ProteinModelPortaliQ91VZ6.
SMRiQ91VZ6. Positions 9-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221043. 11 interactions.
IntActiQ91VZ6. 14 interactions.
MINTiMINT-4121188.
STRINGi10090.ENSMUSP00000027339.

PTM databases

iPTMnetiQ91VZ6.
PhosphoSiteiQ91VZ6.

Proteomic databases

EPDiQ91VZ6.
MaxQBiQ91VZ6.
PaxDbiQ91VZ6.
PeptideAtlasiQ91VZ6.
PRIDEiQ91VZ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027339; ENSMUSP00000027339; ENSMUSG00000026155.
GeneIDi98366.
KEGGimmu:98366.
UCSCiuc007amg.2. mouse.

Organism-specific databases

CTDi60682.
MGIiMGI:2138261. Smap1.

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00820000127006.
HOGENOMiHOG000230583.
HOVERGENiHBG055260.
InParanoidiQ91VZ6.
KOiK12486.
OMAiGMPNGFT.
OrthoDBiEOG78M02F.
PhylomeDBiQ91VZ6.
TreeFamiTF313876.

Miscellaneous databases

PROiQ91VZ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VZ6.
CleanExiMM_SMAP1.
ExpressionAtlasiQ91VZ6. baseline and differential.
GenevisibleiQ91VZ6. MM.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain, Jaw, Limb and Mammary tumor.
  2. "Involvement of stromal membrane-associated protein (SMAP-1) in erythropoietic microenvironment."
    Sato Y., Hong H.N., Yanai N., Obinata M.
    J. Biochem. 124:209-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  3. "A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis."
    Tanabe K., Torii T., Natsume W., Braesch-Andersen S., Watanabe T., Satake M.
    Mol. Biol. Cell 16:1617-1628(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARF6 AND CLATHRIN HEAVY CHAINS, MUTAGENESIS OF ARG-61 AND 192-LEU--ASP-196.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiSMAP1_MOUSE
AccessioniPrimary (citable) accession number: Q91VZ6
Secondary accession number(s): Q497I6, Q68EF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.