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Protein

Zinc finger protein with KRAB and SCAN domains 3

Gene

Zkscan3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri313 – 33523C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri397 – 41923C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri425 – 44723C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri507 – 52923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Autophagy, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein with KRAB and SCAN domains 3
Alternative name(s):
SCAN-KRAB-zinc finger protein
Zinc finger protein 306
Zinc finger protein 307
Zinc finger protein 47 homolog
Short name:
Zf47
Short name:
Zfp-47
Gene namesi
Name:Zkscan3
Synonyms:Skz1, Zfp306, Zfp307, Zfp47
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919989. Zkscan3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation
  • Cytoplasm By similarity

  • Note: Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Zinc finger protein with KRAB and SCAN domains 3PRO_0000394861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331PhosphoserineCombined sources
Modified residuei44 – 441PhosphoserineCombined sources
Modified residuei136 – 1361PhosphothreonineCombined sources
Modified residuei206 – 2061PhosphothreonineBy similarity
Modified residuei223 – 2231PhosphoserineCombined sources
Modified residuei448 – 4481PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91VW9.
PaxDbiQ91VW9.
PRIDEiQ91VW9.

PTM databases

iPTMnetiQ91VW9.
PhosphoSiteiQ91VW9.

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000021327.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068424.

Structurei

3D structure databases

ProteinModelPortaliQ91VW9.
SMRiQ91VW9. Positions 43-135, 281-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 13383SCAN boxPROSITE-ProRule annotationAdd
BLAST
Domaini213 – 27361KRABAdd
BLAST

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.Curated
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri313 – 33523C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri397 – 41923C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri425 – 44723C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri507 – 52923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ91VW9.
KOiK09229.
PhylomeDBiQ91VW9.
TreeFamiTF350830.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 7 hits.
InterProiIPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARESRESTT LDSHSAEDQM ELLVIKVEQE ESSPLAEETS WLGSPGPDRS
60 70 80 90 100
RQRFRAFRYP EAAGPRQALS RLRELCRQWL RPDMHSKEQI LELLVLEQFL
110 120 130 140 150
TILPGELQAW VREQHPDSGE EVVALLEYLD RQLDDTPPQV PDDDDGQELL
160 170 180 190 200
CSKAVLLTSA QGSESSQMEP VEPLLKQESL GSLPSEVRVT HVGHCGEDGV
210 220 230 240 250
TATRLTSELQ GLLKMEDVAP VLSPRWTEQD SSQMNLYKDG MQEHSGSLVS
260 270 280 290 300
LDQDMQTKVR DLPRAEEYRD QKPEQTVCFL GEDTVPIPTG AEASEQEGKL
310 320 330 340 350
QAAQKSATGT RRFYCRECGK SFAQSSGLSK HKRIHTGLKP YECEECGKAF
360 370 380 390 400
IGSSALIIHQ RVHTGEKPYE CEECGKAFSH SSDLIKHQRT HTGEKPYECD
410 420 430 440 450
DCGKTFTQSC SLLEHHRIHT GEKPYQCNMC PKAFRRSSHL LRHQRTHTGD
460 470 480 490 500
KDFFVPEPYW ESQSRVESHW ENIETPVSYQ CNDCERSFSR ITSLIEHQKV
510 520 530 540 550
HTGEKPFECQ TCGKGFTRPS YLIQHQRRHT GKKTSVTVTP AVHSEVGVQL

SLN
Length:553
Mass (Da):62,642
Last modified:December 1, 2001 - v1
Checksum:i53B41483D21AC3C8
GO

Sequence cautioni

The sequence AAG00602 differs from that shown. Reason: Frameshift at positions 421 and 432. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321S → A in BAC27333 (PubMed:16141072).Curated
Sequence conflicti32 – 321S → A in BAE20446 (PubMed:16141072).Curated
Sequence conflicti62 – 621A → T in AAG00602 (PubMed:11342224).Curated
Sequence conflicti67 – 671Q → R in BAE20446 (PubMed:16141072).Curated
Sequence conflicti538 – 5381V → M in AAG00602 (PubMed:11342224).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF291722 mRNA. Translation: AAG00602.1. Frameshift.
AK028902 mRNA. Translation: BAE20446.1.
AK031286 mRNA. Translation: BAC27333.1.
CH466561 Genomic DNA. Translation: EDL32657.1.
CH466561 Genomic DNA. Translation: EDL32658.1.
CH466561 Genomic DNA. Translation: EDL32661.1.
BC007473 mRNA. Translation: AAH07473.1.
CCDSiCCDS26274.1.
RefSeqiNP_001139250.1. NM_001145778.1.
NP_076174.3. NM_023685.4.
XP_006516852.1. XM_006516789.2.
XP_006516853.1. XM_006516790.2.
UniGeneiMm.296071.

Genome annotation databases

GeneIDi72739.
KEGGimmu:72739.
UCSCiuc007pqg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF291722 mRNA. Translation: AAG00602.1. Frameshift.
AK028902 mRNA. Translation: BAE20446.1.
AK031286 mRNA. Translation: BAC27333.1.
CH466561 Genomic DNA. Translation: EDL32657.1.
CH466561 Genomic DNA. Translation: EDL32658.1.
CH466561 Genomic DNA. Translation: EDL32661.1.
BC007473 mRNA. Translation: AAH07473.1.
CCDSiCCDS26274.1.
RefSeqiNP_001139250.1. NM_001145778.1.
NP_076174.3. NM_023685.4.
XP_006516852.1. XM_006516789.2.
XP_006516853.1. XM_006516790.2.
UniGeneiMm.296071.

3D structure databases

ProteinModelPortaliQ91VW9.
SMRiQ91VW9. Positions 43-135, 281-533.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068424.

PTM databases

iPTMnetiQ91VW9.
PhosphoSiteiQ91VW9.

Proteomic databases

MaxQBiQ91VW9.
PaxDbiQ91VW9.
PRIDEiQ91VW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72739.
KEGGimmu:72739.
UCSCiuc007pqg.2. mouse.

Organism-specific databases

CTDi80317.
MGIiMGI:1919989. Zkscan3.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ91VW9.
KOiK09229.
PhylomeDBiQ91VW9.
TreeFamiTF350830.

Miscellaneous databases

PROiQ91VW9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021327.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 7 hits.
InterProiIPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 7 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZKSC3_MOUSE
AccessioniPrimary (citable) accession number: Q91VW9
Secondary accession number(s): Q3V3Z2, Q8CD81, Q9ESY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.