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Protein

Carbonyl reductase family member 4

Gene

Cbr4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The heterotetramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis. Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD. The homotetramer has NADPH-dependent quinone reductase activity. Both homotetramer and the heterotetramer have broad in vitro substrate specificity and can reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones.By similarity

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56NADPBy similarity1
Binding sitei134SubstrateBy similarity1
Active sitei147Proton acceptorPROSITE-ProRule annotation1
Binding sitei147NADPBy similarity1
Binding sitei151NADPBy similarity1
Sitei168Important for interaction with acyl carrier protein (ACP)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 14NADPBy similarity4
Nucleotide bindingi34 – 35NADPBy similarity2
Nucleotide bindingi83 – 85NADPBy similarity3
Nucleotide bindingi180 – 182NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonyl reductase family member 4 (EC:1.-.-.-)
Alternative name(s):
3-ketoacyl-[acyl-carrier-protein] reductase beta subunitBy similarity
Short name:
KAR beta subunitBy similarity
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-By similarity)
Quinone reductase CBR4
Gene namesi
Name:Cbr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2384567. Cbr4.

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
  • oxidoreductase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003198791 – 236Carbonyl reductase family member 4Add BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei40N6-acetyllysineCombined sources1
Modified residuei96N6-acetyllysineCombined sources1
Modified residuei194N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91VT4.
MaxQBiQ91VT4.
PaxDbiQ91VT4.
PeptideAtlasiQ91VT4.
PRIDEiQ91VT4.

PTM databases

iPTMnetiQ91VT4.
PhosphoSitePlusiQ91VT4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031641.
CleanExiMM_CBR4.
ExpressionAtlasiQ91VT4. baseline and differential.
GenevisibleiQ91VT4. MM.

Interactioni

Subunit structurei

Homotetramer (in vitro). Heterotetramer with HSD17B8; contains two molecules each of HSD17B8 and CBR4. Does not form homotetramers when HSD17B8 is coexpressed, only heterotetramers (in vitro).By similarity

Protein-protein interaction databases

IntActiQ91VT4. 2 interactors.
MINTiMINT-4121071.
STRINGi10090.ENSMUSP00000034058.

Structurei

3D structure databases

ProteinModelPortaliQ91VT4.
SMRiQ91VT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ91VT4.
KOiK11539.
OMAiRIWEMSE.
OrthoDBiEOG091G0N2V.
PhylomeDBiQ91VT4.
TreeFamiTF354265.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVCAVFGG SRGIGRAVAQ LMAQKGYRLA IVSRNLEVAK VTAGELGGNH
60 70 80 90 100
LAFRCDVAKE QDVQSTFQEM EKHLGPVNFL VNAAGINRDS LLVRTKTEDM
110 120 130 140 150
ISQLHTNLLG SMLTCKAAMK TMIQQGGSIV NVGSIIGLKG NVGQSAYSAT
160 170 180 190 200
KGGLVGFSRS LAKEVARKKI RVNVVAPGFI RTDMTRHLKE EHFKKNIPLG
210 220 230
RFGETLEVAH AVVFLLESPY ITGHVLIVDG GLQLTV
Length:236
Mass (Da):25,415
Last modified:February 26, 2008 - v2
Checksum:i546F400177DDC7F1
GO

Sequence cautioni

The sequence BAC31519 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89D → G in AAH09118 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043313 mRNA. Translation: BAC31519.1. Different initiation.
AK150763 mRNA. Translation: BAE29830.1.
BC009118 mRNA. Translation: AAH09118.1.
CCDSiCCDS40349.1.
RefSeqiNP_663570.2. NM_145595.2.
UniGeneiMm.29059.

Genome annotation databases

EnsembliENSMUST00000034058; ENSMUSP00000034058; ENSMUSG00000031641.
GeneIDi234309.
KEGGimmu:234309.
UCSCiuc009lub.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043313 mRNA. Translation: BAC31519.1. Different initiation.
AK150763 mRNA. Translation: BAE29830.1.
BC009118 mRNA. Translation: AAH09118.1.
CCDSiCCDS40349.1.
RefSeqiNP_663570.2. NM_145595.2.
UniGeneiMm.29059.

3D structure databases

ProteinModelPortaliQ91VT4.
SMRiQ91VT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91VT4. 2 interactors.
MINTiMINT-4121071.
STRINGi10090.ENSMUSP00000034058.

PTM databases

iPTMnetiQ91VT4.
PhosphoSitePlusiQ91VT4.

Proteomic databases

EPDiQ91VT4.
MaxQBiQ91VT4.
PaxDbiQ91VT4.
PeptideAtlasiQ91VT4.
PRIDEiQ91VT4.

Protocols and materials databases

DNASUi234309.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034058; ENSMUSP00000034058; ENSMUSG00000031641.
GeneIDi234309.
KEGGimmu:234309.
UCSCiuc009lub.2. mouse.

Organism-specific databases

CTDi84869.
MGIiMGI:2384567. Cbr4.

Phylogenomic databases

eggNOGiKOG1200. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG002145.
InParanoidiQ91VT4.
KOiK11539.
OMAiRIWEMSE.
OrthoDBiEOG091G0N2V.
PhylomeDBiQ91VT4.
TreeFamiTF354265.

Enzyme and pathway databases

UniPathwayiUPA00094.
ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.

Miscellaneous databases

PROiQ91VT4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031641.
CleanExiMM_CBR4.
ExpressionAtlasiQ91VT4. baseline and differential.
GenevisibleiQ91VT4. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBR4_MOUSE
AccessioniPrimary (citable) accession number: Q91VT4
Secondary accession number(s): Q3UBY4, Q8BXV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.