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Protein

Carbonyl reductase family member 4

Gene

Cbr4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The heterotetramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis. Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD. The homotetramer has NADPH-dependent quinone reductase activity. Both homotetramer and the heterotetramer have broad in vitro substrate specificity and can reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones.By similarity

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56NADPBy similarity1
Binding sitei134SubstrateBy similarity1
Active sitei147Proton acceptorPROSITE-ProRule annotation1
Binding sitei147NADPBy similarity1
Binding sitei151NADPBy similarity1
Sitei168Important for interaction with acyl carrier protein (ACP)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 14NADPBy similarity4
Nucleotide bindingi34 – 35NADPBy similarity2
Nucleotide bindingi83 – 85NADPBy similarity3
Nucleotide bindingi180 – 182NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNAD, NADP

Enzyme and pathway databases

ReactomeiR-MMU-75105 Fatty acyl-CoA biosynthesis
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonyl reductase family member 4 (EC:1.-.-.-)
Alternative name(s):
3-ketoacyl-[acyl-carrier-protein] reductase beta subunitBy similarity
Short name:
KAR beta subunitBy similarity
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-By similarity)
Quinone reductase CBR4
Gene namesi
Name:Cbr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2384567 Cbr4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003198791 – 236Carbonyl reductase family member 4Add BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei40N6-acetyllysineCombined sources1
Modified residuei96N6-acetyllysineCombined sources1
Modified residuei194N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91VT4
MaxQBiQ91VT4
PaxDbiQ91VT4
PeptideAtlasiQ91VT4
PRIDEiQ91VT4

PTM databases

iPTMnetiQ91VT4
PhosphoSitePlusiQ91VT4

Expressioni

Gene expression databases

BgeeiENSMUSG00000031641
CleanExiMM_CBR4
ExpressionAtlasiQ91VT4 baseline and differential
GenevisibleiQ91VT4 MM

Interactioni

Subunit structurei

Homotetramer (in vitro). Heterotetramer with HSD17B8; contains two molecules each of HSD17B8 and CBR4. Does not form homotetramers when HSD17B8 is coexpressed, only heterotetramers (in vitro).By similarity

Protein-protein interaction databases

IntActiQ91VT4, 2 interactors
MINTiQ91VT4
STRINGi10090.ENSMUSP00000034058

Structurei

3D structure databases

ProteinModelPortaliQ91VT4
SMRiQ91VT4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1200 Eukaryota
COG1028 LUCA
GeneTreeiENSGT00910000144076
HOVERGENiHBG002145
InParanoidiQ91VT4
KOiK11539
OMAiEHDVQNT
OrthoDBiEOG091G0N2V
PhylomeDBiQ91VT4
TreeFamiTF354265

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

Q91VT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVCAVFGG SRGIGRAVAQ LMAQKGYRLA IVSRNLEVAK VTAGELGGNH
60 70 80 90 100
LAFRCDVAKE QDVQSTFQEM EKHLGPVNFL VNAAGINRDS LLVRTKTEDM
110 120 130 140 150
ISQLHTNLLG SMLTCKAAMK TMIQQGGSIV NVGSIIGLKG NVGQSAYSAT
160 170 180 190 200
KGGLVGFSRS LAKEVARKKI RVNVVAPGFI RTDMTRHLKE EHFKKNIPLG
210 220 230
RFGETLEVAH AVVFLLESPY ITGHVLIVDG GLQLTV
Length:236
Mass (Da):25,415
Last modified:February 26, 2008 - v2
Checksum:i546F400177DDC7F1
GO

Sequence cautioni

The sequence BAC31519 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89D → G in AAH09118 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043313 mRNA Translation: BAC31519.1 Different initiation.
AK150763 mRNA Translation: BAE29830.1
BC009118 mRNA Translation: AAH09118.1
CCDSiCCDS40349.1
RefSeqiNP_663570.2, NM_145595.2
UniGeneiMm.29059

Genome annotation databases

EnsembliENSMUST00000034058; ENSMUSP00000034058; ENSMUSG00000031641
GeneIDi234309
KEGGimmu:234309
UCSCiuc009lub.2 mouse

Entry informationi

Entry nameiCBR4_MOUSE
AccessioniPrimary (citable) accession number: Q91VT4
Secondary accession number(s): Q3UBY4, Q8BXV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 23, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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