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Protein

FERM, RhoGEF and pleckstrin domain-containing protein 2

Gene

Farp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as guanine nucleotide exchange factor that activates RAC1. May have relatively low activity (PubMed:23375260 and PubMed:20702777). Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion.4 Publications

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton reorganization Source: UniProtKB
  • cell adhesion Source: UniProtKB
  • neuron remodeling Source: MGI
  • osteoclast differentiation Source: UniProtKB
  • podosome assembly Source: UniProtKB
  • Rac protein signal transduction Source: MGI
  • regulation of integrin activation Source: UniProtKB
  • regulation of Rho protein signal transduction Source: InterPro
  • semaphorin-plexin signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-399955. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.

Names & Taxonomyi

Protein namesi
Recommended name:
FERM, RhoGEF and pleckstrin domain-containing protein 2
Alternative name(s):
FERM domain including RhoGEF
Short name:
FIR
Gene namesi
Name:Farp2
Synonyms:Kiaa0793
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2385126. Farp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi730 – 7301L → R: Increases guanyl-nucleotide exchange factor activity with RAC1; when associated with Q-733. 1 Publication
Mutagenesisi733 – 7331L → Q: Increases guanyl-nucleotide exchange factor activity with RAC1; when associated with R-730. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065FERM, RhoGEF and pleckstrin domain-containing protein 2PRO_0000232756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei389 – 3891PhosphoserineBy similarity
Modified residuei440 – 4401PhosphoserineBy similarity
Modified residuei863 – 8631PhosphoserineCombined sources
Modified residuei880 – 8801PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91VS8.
PaxDbiQ91VS8.
PeptideAtlasiQ91VS8.
PRIDEiQ91VS8.

PTM databases

iPTMnetiQ91VS8.
PhosphoSiteiQ91VS8.

Expressioni

Tissue specificityi

Detected in adult brain, lung and testis. Detected in embryonic hippocampus and brain cortex.1 Publication

Inductioni

Up-regulated by TNFSF11.1 Publication

Gene expression databases

BgeeiENSMUSG00000034066.
CleanExiMM_FARP2.
ExpressionAtlasiQ91VS8. baseline and differential.
GenevisibleiQ91VS8. MM.

Interactioni

Subunit structurei

Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. Interacts with RAC1.3 Publications

Protein-protein interaction databases

IntActiQ91VS8. 2 interactions.
MINTiMINT-8178498.
STRINGi10090.ENSMUSP00000043545.

Structurei

Secondary structure

1
1065
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi538 – 56124Combined sources
Helixi563 – 5708Combined sources
Helixi576 – 5838Combined sources
Turni584 – 5863Combined sources
Helixi587 – 60822Combined sources
Helixi613 – 6197Combined sources
Helixi623 – 63311Combined sources
Helixi634 – 6363Combined sources
Helixi637 – 6415Combined sources
Helixi643 – 65513Combined sources
Helixi658 – 66811Combined sources
Helixi677 – 6804Combined sources
Helixi683 – 70119Combined sources
Helixi709 – 72517Combined sources
Helixi727 – 74317Combined sources
Beta strandi746 – 7483Combined sources
Beta strandi759 – 76810Combined sources
Beta strandi773 – 7819Combined sources
Beta strandi784 – 7885Combined sources
Beta strandi799 – 8068Combined sources
Beta strandi810 – 8134Combined sources
Turni817 – 8226Combined sources
Beta strandi823 – 8275Combined sources
Beta strandi832 – 8365Combined sources
Helixi840 – 85617Combined sources
Helixi909 – 9146Combined sources
Turni915 – 9173Combined sources
Beta strandi919 – 9213Combined sources
Helixi922 – 9287Combined sources
Beta strandi933 – 9397Combined sources
Beta strandi948 – 9558Combined sources
Beta strandi958 – 9647Combined sources
Beta strandi971 – 9755Combined sources
Beta strandi980 – 9823Combined sources
Beta strandi994 – 10007Combined sources
Beta strandi1003 – 10086Combined sources
Beta strandi1010 – 10123Combined sources
Helixi1013 – 102311Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GYVX-ray2.90A/B/C/D/E/F/G/H/I536-749[»]
4GZUX-ray3.20A/B536-1032[»]
ProteinModelPortaliQ91VS8.
SMRiQ91VS8. Positions 47-321, 533-1026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 324281FERMPROSITE-ProRule annotationAdd
BLAST
Domaini538 – 729192DHPROSITE-ProRule annotationAdd
BLAST
Domaini758 – 85598PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini930 – 102798PH 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi397 – 49195Pro/Ser-richAdd
BLAST

Domaini

Intramolecular interaction between the DH domain and the PH domains can stabilize the protein in an autoinhibited conformation.1 Publication

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3531. Eukaryota.
ENOG410XP7Q. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007957.
HOVERGENiHBG081521.
InParanoidiQ91VS8.
KOiK06082.
OMAiMHVCWYR.
OrthoDBiEOG091G015W.
TreeFamiTF351276.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 3 hits.
InterProiIPR019749. Band_41_domain.
IPR000219. DH-domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS00660. FERM_1. 1 hit.
PS50057. FERM_3. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEIEGTYRA LPTSGTRLGG QTAIGVSTLE PEQSLSPRMQ EKHMRIRVKL
60 70 80 90 100
LDSTVELFDI EPKCDGQVLL TQVWKHLNLI ECDYFGLEFK NVQSYWIWLE
110 120 130 140 150
PMKPIIRQVR KPKNAVLRLA VKFFPPDPGQ LQEEYTRYLF ALQLKRDLLE
160 170 180 190 200
ERLTCTANTA ALLISHLLQS EIGDYDETLD REHLKANEYL PNQEKSLEKI
210 220 230 240 250
LDFHQRHTGQ TPAESDFQVL EIARKLEMYG IRFHMASDRE GTKINLAVSH
260 270 280 290 300
MGVLVFQGTT KINTFNWSKV RKLSFKRKRF LIKLHPEVHG PYQDTLEFLL
310 320 330 340 350
GSRDECKNFW KICVEYHTFF RLSDQPKPKA KAVFFSRGSS FRYSGRTQKQ
360 370 380 390 400
LVDYVKDGGM KRIPYERRHS KTRTSLHALT VDLPKQSVSF TDGLRTSASL
410 420 430 440 450
SSANVSFYPP PSSSLSPPGL PNLKDSSSSL VDPQAPVIKS TAAERSSGPS
460 470 480 490 500
SSDGPSTQSA HLPGPPVLRP GPGFSMDSPQ PSPSSLKSHL SLCPELQAAL
510 520 530 540 550
STAEQGASPV LSPVLSGAGT ARMDNQEEQK HKHMPEDEAY FIAKEILATE
560 570 580 590 600
RTYLKDLEVI TVWFRSVLIK EEAMPAALMA LLFSNIDPVY EFHRGFLHEV
610 620 630 640 650
EQRLALWEGP SSAHLKGDHQ RIGDILLRNM RQLKEFTSYF QRHDEVLTEL
660 670 680 690 700
EKATKHCKKL EAVYKEFELQ KVCYLPLNTF LLKPVQRLVH YRLLLSRLCA
710 720 730 740 750
HYSPGHRDYA DCHEALKAIT EVTTELQQSL TRLENLQKLT ELQRDLVGVE
760 770 780 790 800
NLIAPGREFI REGCLHKLTK KGLQQRMFFL FSDMLLYTSK SVTGASHFRI
810 820 830 840 850
RGFLPLRGML VEESENEWSV PHCFTIYAAQ KTIVVAASTR LEKEKWMQDL
860 870 880 890 900
NAAIQAAKTI GDSPPVLLGG PVYTRTPRSS DEVSLEESED GRGNRGSLEG
910 920 930 940 950
NSQHRANTTM HVCWYRNTSV SRADHSAAVE NQLSGYLLRK FKNSNGWQKL
960 970 980 990 1000
WVVFTNFCLF FYKTHQDDYP LASLPLLGYS VSLPREADSI HKDYVFKLQF
1010 1020 1030 1040 1050
KSHVYFFRAE SKYTFERWMD VIKRASSSPG RPPSFTQDCS HHSPGLEAEI
1060
REKEACPSPC LDKNL
Length:1,065
Mass (Da):121,281
Last modified:July 27, 2011 - v2
Checksum:i452F3BB652BE99D9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2341H → R in BAE42626 (PubMed:16141072).Curated
Sequence conflicti821 – 8211P → L in BAE42626 (PubMed:16141072).Curated
Sequence conflicti821 – 8211P → L in AAH09153 (PubMed:15489334).Curated
Sequence conflicti1019 – 10191M → I in BAD32306 (PubMed:15368895).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK171713 mRNA. Translation: BAE42626.1.
AC108412 Genomic DNA. No translation available.
AC131316 Genomic DNA. No translation available.
BC009153 mRNA. Translation: AAH09153.1.
AK173028 mRNA. Translation: BAD32306.1.
CCDSiCCDS15191.1.
RefSeqiNP_663494.2. NM_145519.2.
UniGeneiMm.243091.

Genome annotation databases

EnsembliENSMUST00000120301; ENSMUSP00000112725; ENSMUSG00000034066.
GeneIDi227377.
KEGGimmu:227377.
UCSCiuc007cec.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK171713 mRNA. Translation: BAE42626.1.
AC108412 Genomic DNA. No translation available.
AC131316 Genomic DNA. No translation available.
BC009153 mRNA. Translation: AAH09153.1.
AK173028 mRNA. Translation: BAD32306.1.
CCDSiCCDS15191.1.
RefSeqiNP_663494.2. NM_145519.2.
UniGeneiMm.243091.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GYVX-ray2.90A/B/C/D/E/F/G/H/I536-749[»]
4GZUX-ray3.20A/B536-1032[»]
ProteinModelPortaliQ91VS8.
SMRiQ91VS8. Positions 47-321, 533-1026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91VS8. 2 interactions.
MINTiMINT-8178498.
STRINGi10090.ENSMUSP00000043545.

PTM databases

iPTMnetiQ91VS8.
PhosphoSiteiQ91VS8.

Proteomic databases

MaxQBiQ91VS8.
PaxDbiQ91VS8.
PeptideAtlasiQ91VS8.
PRIDEiQ91VS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120301; ENSMUSP00000112725; ENSMUSG00000034066.
GeneIDi227377.
KEGGimmu:227377.
UCSCiuc007cec.2. mouse.

Organism-specific databases

CTDi9855.
MGIiMGI:2385126. Farp2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3531. Eukaryota.
ENOG410XP7Q. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000007957.
HOVERGENiHBG081521.
InParanoidiQ91VS8.
KOiK06082.
OMAiMHVCWYR.
OrthoDBiEOG091G015W.
TreeFamiTF351276.

Enzyme and pathway databases

ReactomeiR-MMU-399955. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.

Miscellaneous databases

PROiQ91VS8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034066.
CleanExiMM_FARP2.
ExpressionAtlasiQ91VS8. baseline and differential.
GenevisibleiQ91VS8. MM.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 3 hits.
InterProiIPR019749. Band_41_domain.
IPR000219. DH-domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS00660. FERM_1. 1 hit.
PS50057. FERM_3. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFARP2_MOUSE
AccessioniPrimary (citable) accession number: Q91VS8
Secondary accession number(s): E9QJS4, Q3TAP2, Q69ZZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.