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Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38GlutathioneBy similarity1
Sitei50Activates the enzyme when modifiedBy similarity1
Binding sitei73GlutathioneBy similarity1
Binding sitei74GlutathioneBy similarity1
Binding sitei76GlutathioneBy similarity1
Binding sitei81GlutathioneBy similarity1
Binding sitei121GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione peroxidase activity Source: MGI
  • glutathione transferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-MMU-156590 Glutathione conjugation
R-MMU-5423646 Aflatoxin activation and detoxification
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:Mgst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1913850 Mgst1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9LumenalBy similarity7
Transmembranei10 – 33HelicalBy similarityAdd BLAST24
Topological domaini34 – 62CytoplasmicBy similarityAdd BLAST29
Transmembranei63 – 96HelicalBy similarityAdd BLAST34
Topological domaini97 – 99LumenalBy similarity3
Transmembranei100 – 123HelicalBy similarityAdd BLAST24
Topological domaini124 – 128CytoplasmicBy similarity5
Transmembranei129 – 148HelicalBy similarityAdd BLAST20
Topological domaini149 – 155LumenalBy similarity7

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177371 – 155Microsomal glutathione S-transferase 1Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei55N6-acetyllysineCombined sources1
Modified residuei60N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-42 and Lys-55 is observed in liver mitochondria from fasted mice but not from fed mice.
Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

MaxQBiQ91VS7
PaxDbiQ91VS7
PRIDEiQ91VS7

PTM databases

iPTMnetiQ91VS7
PhosphoSitePlusiQ91VS7
SwissPalmiQ91VS7

Expressioni

Gene expression databases

BgeeiENSMUSG00000008540
CleanExiMM_MGST1
ExpressionAtlasiQ91VS7 baseline and differential
GenevisibleiQ91VS7 MM

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Protein-protein interaction databases

BioGridi208104, 1 interactor
IntActiQ91VS7, 2 interactors
MINTiQ91VS7
STRINGi10090.ENSMUSP00000008684

Structurei

3D structure databases

ProteinModelPortaliQ91VS7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1 Eukaryota
ENOG4111VJG LUCA
GeneTreeiENSGT00390000011980
HOGENOMiHOG000231759
HOVERGENiHBG052470
InParanoidiQ91VS7
PhylomeDBiQ91VS7
TreeFamiTF105327

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
SUPFAMiSSF161084 SSF161084, 1 hit

Sequencei

Sequence statusi: Complete.

Q91VS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLRQLMDN EVLMAFTSYA TIILTKMMFM SSATAFQRIT NKVFANPEDC
60 70 80 90 100
AGFGKGENAK KFVRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STALMHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR

SRLYL
Length:155
Mass (Da):17,552
Last modified:January 23, 2007 - v3
Checksum:iC1E202663496C762
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5R → K in AAD51096 (Ref. 1) Curated1
Sequence conflicti56G → V in AAH09155 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159050 mRNA Translation: AAD51096.1
AK002800 mRNA Translation: BAB22368.1
AK005122 mRNA Translation: BAB23827.1
BC009155 mRNA Translation: AAH09155.1
CCDSiCCDS20668.1
RefSeqiNP_064330.2, NM_019946.4
UniGeneiMm.14796

Genome annotation databases

EnsembliENSMUST00000008684; ENSMUSP00000008684; ENSMUSG00000008540
ENSMUST00000120230; ENSMUSP00000113859; ENSMUSG00000008540
ENSMUST00000120302; ENSMUSP00000113257; ENSMUSG00000008540
GeneIDi56615
UCSCiuc009enk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMGST1_MOUSE
AccessioniPrimary (citable) accession number: Q91VS7
Secondary accession number(s): Q9CQ57, Q9R191
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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