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Q91VS7

- MGST1_MOUSE

UniProt

Q91VS7 - MGST1_MOUSE

Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Enzyme regulationi

    Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381GlutathioneBy similarity
    Sitei50 – 501Activates the enzyme when modifiedBy similarity
    Binding sitei73 – 731GlutathioneBy similarity
    Binding sitei74 – 741GlutathioneBy similarity
    Binding sitei76 – 761GlutathioneBy similarity
    Binding sitei81 – 811GlutathioneBy similarity
    Binding sitei121 – 1211GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione binding Source: Ensembl
    2. glutathione peroxidase activity Source: Ensembl
    3. glutathione transferase activity Source: MGI

    GO - Biological processi

    1. cellular response to lipid hydroperoxide Source: Ensembl
    2. glutathione metabolic process Source: MGI
    3. Leydig cell differentiation Source: Ensembl
    4. protein homotrimerization Source: UniProtKB
    5. response to drug Source: Ensembl
    6. response to lipopolysaccharide Source: Ensembl
    7. response to organonitrogen compound Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    ReactomeiREACT_215316. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
    Short name:
    Microsomal GST-1
    Alternative name(s):
    Microsomal GST-I
    Gene namesi
    Name:Mgst1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1913850. Mgst1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical part of cell Source: Ensembl
    2. endoplasmic reticulum Source: UniProtKB
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB
    5. mitochondrial inner membrane Source: MGI
    6. mitochondrial outer membrane Source: UniProtKB-SubCell
    7. mitochondrion Source: MGI
    8. nucleus Source: Ensembl
    9. peroxisomal membrane Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 155154Microsomal glutathione S-transferase 1PRO_0000217737Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421N6-acetyllysine1 Publication
    Modified residuei55 – 551N6-acetyllysine1 Publication
    Modified residuei60 – 601N6-acetyllysine1 Publication

    Post-translational modificationi

    Acetylation of Lys-42 and Lys-55 is observed in liver mitochondria from fasted mice but not from fed mice.1 Publication
    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

    Keywords - PTMi

    Acetylation, Nitration

    Proteomic databases

    MaxQBiQ91VS7.
    PaxDbiQ91VS7.
    PRIDEiQ91VS7.

    PTM databases

    PhosphoSiteiQ91VS7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91VS7.
    BgeeiQ91VS7.
    CleanExiMM_MGST1.
    GenevestigatoriQ91VS7.

    Interactioni

    Subunit structurei

    Homotrimer; The trimer binds only one molecule of glutathione.By similarity

    Protein-protein interaction databases

    IntActiQ91VS7. 2 interactions.
    MINTiMINT-1840438.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91VS7.
    SMRiQ91VS7. Positions 10-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini3 – 97LumenalBy similarity
    Topological domaini34 – 6229CytoplasmicBy similarityAdd
    BLAST
    Topological domaini97 – 993LumenalBy similarity
    Topological domaini124 – 1285CytoplasmicBy similarity
    Topological domaini149 – 1557LumenalBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3324HelicalBy similarityAdd
    BLAST
    Transmembranei63 – 9634HelicalBy similarityAdd
    BLAST
    Transmembranei100 – 12324HelicalBy similarityAdd
    BLAST
    Transmembranei129 – 14820HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG71159.
    GeneTreeiENSGT00390000011980.
    HOGENOMiHOG000231759.
    HOVERGENiHBG052470.
    InParanoidiQ91VS7.
    KOiK00799.
    OrthoDBiEOG7288T0.
    PhylomeDBiQ91VS7.
    TreeFamiTF105327.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91VS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLRQLMDN EVLMAFTSYA TIILTKMMFM SSATAFQRIT NKVFANPEDC    50
    AGFGKGENAK KFVRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL 100
    STALMHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR 150
    SRLYL 155
    Length:155
    Mass (Da):17,552
    Last modified:January 23, 2007 - v3
    Checksum:iC1E202663496C762
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51R → K in AAD51096. 1 PublicationCurated
    Sequence conflicti56 – 561G → V in AAH09155. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159050 mRNA. Translation: AAD51096.1.
    AK002800 mRNA. Translation: BAB22368.1.
    AK005122 mRNA. Translation: BAB23827.1.
    BC009155 mRNA. Translation: AAH09155.1.
    CCDSiCCDS20668.1.
    RefSeqiNP_064330.2. NM_019946.4.
    UniGeneiMm.14796.

    Genome annotation databases

    EnsembliENSMUST00000008684; ENSMUSP00000008684; ENSMUSG00000008540.
    ENSMUST00000120230; ENSMUSP00000113859; ENSMUSG00000008540.
    ENSMUST00000120302; ENSMUSP00000113257; ENSMUSG00000008540.
    GeneIDi56615.
    KEGGimmu:56615.
    UCSCiuc009enk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159050 mRNA. Translation: AAD51096.1 .
    AK002800 mRNA. Translation: BAB22368.1 .
    AK005122 mRNA. Translation: BAB23827.1 .
    BC009155 mRNA. Translation: AAH09155.1 .
    CCDSi CCDS20668.1.
    RefSeqi NP_064330.2. NM_019946.4.
    UniGenei Mm.14796.

    3D structure databases

    ProteinModelPortali Q91VS7.
    SMRi Q91VS7. Positions 10-148.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91VS7. 2 interactions.
    MINTi MINT-1840438.

    PTM databases

    PhosphoSitei Q91VS7.

    Proteomic databases

    MaxQBi Q91VS7.
    PaxDbi Q91VS7.
    PRIDEi Q91VS7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000008684 ; ENSMUSP00000008684 ; ENSMUSG00000008540 .
    ENSMUST00000120230 ; ENSMUSP00000113859 ; ENSMUSG00000008540 .
    ENSMUST00000120302 ; ENSMUSP00000113257 ; ENSMUSG00000008540 .
    GeneIDi 56615.
    KEGGi mmu:56615.
    UCSCi uc009enk.1. mouse.

    Organism-specific databases

    CTDi 4257.
    MGIi MGI:1913850. Mgst1.

    Phylogenomic databases

    eggNOGi NOG71159.
    GeneTreei ENSGT00390000011980.
    HOGENOMi HOG000231759.
    HOVERGENi HBG052470.
    InParanoidi Q91VS7.
    KOi K00799.
    OrthoDBi EOG7288T0.
    PhylomeDBi Q91VS7.
    TreeFami TF105327.

    Enzyme and pathway databases

    Reactomei REACT_215316. Glutathione conjugation.

    Miscellaneous databases

    ChiTaRSi MGST1. mouse.
    NextBioi 313039.
    PROi Q91VS7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91VS7.
    Bgeei Q91VS7.
    CleanExi MM_MGST1.
    Genevestigatori Q91VS7.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and bacterial expression of full length cDNA for the mouse liver microsomal glutathione S-transferase."
      Raza H., Mullick J., Avadhani N.G.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-42; LYS-55 AND LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiMGST1_MOUSE
    AccessioniPrimary (citable) accession number: Q91VS7
    Secondary accession number(s): Q9CQ57, Q9R191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3