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Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38GlutathioneBy similarity1
Sitei50Activates the enzyme when modifiedBy similarity1
Binding sitei73GlutathioneBy similarity1
Binding sitei74GlutathioneBy similarity1
Binding sitei76GlutathioneBy similarity1
Binding sitei81GlutathioneBy similarity1
Binding sitei121GlutathioneBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-5423646. Aflatoxin activation and detoxification.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:Mgst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1913850. Mgst1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9LumenalBy similarity7
Transmembranei10 – 33HelicalBy similarityAdd BLAST24
Topological domaini34 – 62CytoplasmicBy similarityAdd BLAST29
Transmembranei63 – 96HelicalBy similarityAdd BLAST34
Topological domaini97 – 99LumenalBy similarity3
Transmembranei100 – 123HelicalBy similarityAdd BLAST24
Topological domaini124 – 128CytoplasmicBy similarity5
Transmembranei129 – 148HelicalBy similarityAdd BLAST20
Topological domaini149 – 155LumenalBy similarity7

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002177372 – 155Microsomal glutathione S-transferase 1Add BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei55N6-acetyllysineCombined sources1
Modified residuei60N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-42 and Lys-55 is observed in liver mitochondria from fasted mice but not from fed mice.
Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

MaxQBiQ91VS7.
PaxDbiQ91VS7.
PRIDEiQ91VS7.

PTM databases

iPTMnetiQ91VS7.
PhosphoSitePlusiQ91VS7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000008540.
CleanExiMM_MGST1.
ExpressionAtlasiQ91VS7. baseline and differential.
GenevisibleiQ91VS7. MM.

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Protein-protein interaction databases

BioGridi208104. 1 interactor.
IntActiQ91VS7. 2 interactors.
MINTiMINT-1840438.
STRINGi10090.ENSMUSP00000008684.

Structurei

3D structure databases

ProteinModelPortaliQ91VS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1. Eukaryota.
ENOG4111VJG. LUCA.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiQ91VS7.
KOiK00799.
PhylomeDBiQ91VS7.
TreeFamiTF105327.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLRQLMDN EVLMAFTSYA TIILTKMMFM SSATAFQRIT NKVFANPEDC
60 70 80 90 100
AGFGKGENAK KFVRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STALMHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR

SRLYL
Length:155
Mass (Da):17,552
Last modified:January 23, 2007 - v3
Checksum:iC1E202663496C762
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5R → K in AAD51096 (Ref. 1) Curated1
Sequence conflicti56G → V in AAH09155 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159050 mRNA. Translation: AAD51096.1.
AK002800 mRNA. Translation: BAB22368.1.
AK005122 mRNA. Translation: BAB23827.1.
BC009155 mRNA. Translation: AAH09155.1.
CCDSiCCDS20668.1.
RefSeqiNP_064330.2. NM_019946.4.
UniGeneiMm.14796.

Genome annotation databases

EnsembliENSMUST00000008684; ENSMUSP00000008684; ENSMUSG00000008540.
ENSMUST00000120230; ENSMUSP00000113859; ENSMUSG00000008540.
ENSMUST00000120302; ENSMUSP00000113257; ENSMUSG00000008540.
GeneIDi56615.
KEGGimmu:56615.
UCSCiuc009enk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159050 mRNA. Translation: AAD51096.1.
AK002800 mRNA. Translation: BAB22368.1.
AK005122 mRNA. Translation: BAB23827.1.
BC009155 mRNA. Translation: AAH09155.1.
CCDSiCCDS20668.1.
RefSeqiNP_064330.2. NM_019946.4.
UniGeneiMm.14796.

3D structure databases

ProteinModelPortaliQ91VS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208104. 1 interactor.
IntActiQ91VS7. 2 interactors.
MINTiMINT-1840438.
STRINGi10090.ENSMUSP00000008684.

PTM databases

iPTMnetiQ91VS7.
PhosphoSitePlusiQ91VS7.

Proteomic databases

MaxQBiQ91VS7.
PaxDbiQ91VS7.
PRIDEiQ91VS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008684; ENSMUSP00000008684; ENSMUSG00000008540.
ENSMUST00000120230; ENSMUSP00000113859; ENSMUSG00000008540.
ENSMUST00000120302; ENSMUSP00000113257; ENSMUSG00000008540.
GeneIDi56615.
KEGGimmu:56615.
UCSCiuc009enk.1. mouse.

Organism-specific databases

CTDi4257.
MGIiMGI:1913850. Mgst1.

Phylogenomic databases

eggNOGiENOG410IXE1. Eukaryota.
ENOG4111VJG. LUCA.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiQ91VS7.
KOiK00799.
PhylomeDBiQ91VS7.
TreeFamiTF105327.

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-5423646. Aflatoxin activation and detoxification.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiMgst1. mouse.
PROiQ91VS7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008540.
CleanExiMM_MGST1.
ExpressionAtlasiQ91VS7. baseline and differential.
GenevisibleiQ91VS7. MM.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGST1_MOUSE
AccessioniPrimary (citable) accession number: Q91VS7
Secondary accession number(s): Q9CQ57, Q9R191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.