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Q91VS7

- MGST1_MOUSE

UniProt

Q91VS7 - MGST1_MOUSE

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Protein

Microsomal glutathione S-transferase 1

Gene

Mgst1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381GlutathioneBy similarity
Sitei50 – 501Activates the enzyme when modifiedBy similarity
Binding sitei73 – 731GlutathioneBy similarity
Binding sitei74 – 741GlutathioneBy similarity
Binding sitei76 – 761GlutathioneBy similarity
Binding sitei81 – 811GlutathioneBy similarity
Binding sitei121 – 1211GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione binding Source: Ensembl
  2. glutathione peroxidase activity Source: Ensembl
  3. glutathione transferase activity Source: MGI

GO - Biological processi

  1. cellular response to lipid hydroperoxide Source: Ensembl
  2. glutathione metabolic process Source: MGI
  3. Leydig cell differentiation Source: Ensembl
  4. protein homotrimerization Source: UniProtKB
  5. response to drug Source: Ensembl
  6. response to lipopolysaccharide Source: Ensembl
  7. response to organonitrogen compound Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_215316. Glutathione conjugation.
REACT_237136. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:Mgst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1913850. Mgst1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini3 – 97LumenalBy similarity
Transmembranei10 – 3324HelicalBy similarityAdd
BLAST
Topological domaini34 – 6229CytoplasmicBy similarityAdd
BLAST
Transmembranei63 – 9634HelicalBy similarityAdd
BLAST
Topological domaini97 – 993LumenalBy similarity
Transmembranei100 – 12324HelicalBy similarityAdd
BLAST
Topological domaini124 – 1285CytoplasmicBy similarity
Transmembranei129 – 14820HelicalBy similarityAdd
BLAST
Topological domaini149 – 1557LumenalBy similarity

GO - Cellular componenti

  1. apical part of cell Source: Ensembl
  2. endoplasmic reticulum Source: UniProtKB
  3. integral component of membrane Source: UniProtKB
  4. mitochondrial inner membrane Source: MGI
  5. mitochondrial outer membrane Source: UniProtKB-KW
  6. mitochondrion Source: MGI
  7. nucleus Source: Ensembl
  8. peroxisomal membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 155154Microsomal glutathione S-transferase 1PRO_0000217737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421N6-acetyllysine1 Publication
Modified residuei55 – 551N6-acetyllysine1 Publication
Modified residuei60 – 601N6-acetyllysine1 Publication

Post-translational modificationi

Acetylation of Lys-42 and Lys-55 is observed in liver mitochondria from fasted mice but not from fed mice.1 Publication
Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

MaxQBiQ91VS7.
PaxDbiQ91VS7.
PRIDEiQ91VS7.

PTM databases

PhosphoSiteiQ91VS7.

Expressioni

Gene expression databases

BgeeiQ91VS7.
CleanExiMM_MGST1.
ExpressionAtlasiQ91VS7. baseline and differential.
GenevestigatoriQ91VS7.

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Protein-protein interaction databases

IntActiQ91VS7. 2 interactions.
MINTiMINT-1840438.

Structurei

3D structure databases

ProteinModelPortaliQ91VS7.
SMRiQ91VS7. Positions 10-148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71159.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiQ91VS7.
KOiK00799.
OrthoDBiEOG7288T0.
PhylomeDBiQ91VS7.
TreeFamiTF105327.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADLRQLMDN EVLMAFTSYA TIILTKMMFM SSATAFQRIT NKVFANPEDC
60 70 80 90 100
AGFGKGENAK KFVRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STALMHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGV TLSMAYRLLR

SRLYL
Length:155
Mass (Da):17,552
Last modified:January 23, 2007 - v3
Checksum:iC1E202663496C762
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51R → K in AAD51096. 1 PublicationCurated
Sequence conflicti56 – 561G → V in AAH09155. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159050 mRNA. Translation: AAD51096.1.
AK002800 mRNA. Translation: BAB22368.1.
AK005122 mRNA. Translation: BAB23827.1.
BC009155 mRNA. Translation: AAH09155.1.
CCDSiCCDS20668.1.
RefSeqiNP_064330.2. NM_019946.4.
UniGeneiMm.14796.

Genome annotation databases

EnsembliENSMUST00000008684; ENSMUSP00000008684; ENSMUSG00000008540.
ENSMUST00000120230; ENSMUSP00000113859; ENSMUSG00000008540.
ENSMUST00000120302; ENSMUSP00000113257; ENSMUSG00000008540.
GeneIDi56615.
KEGGimmu:56615.
UCSCiuc009enk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159050 mRNA. Translation: AAD51096.1 .
AK002800 mRNA. Translation: BAB22368.1 .
AK005122 mRNA. Translation: BAB23827.1 .
BC009155 mRNA. Translation: AAH09155.1 .
CCDSi CCDS20668.1.
RefSeqi NP_064330.2. NM_019946.4.
UniGenei Mm.14796.

3D structure databases

ProteinModelPortali Q91VS7.
SMRi Q91VS7. Positions 10-148.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91VS7. 2 interactions.
MINTi MINT-1840438.

PTM databases

PhosphoSitei Q91VS7.

Proteomic databases

MaxQBi Q91VS7.
PaxDbi Q91VS7.
PRIDEi Q91VS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000008684 ; ENSMUSP00000008684 ; ENSMUSG00000008540 .
ENSMUST00000120230 ; ENSMUSP00000113859 ; ENSMUSG00000008540 .
ENSMUST00000120302 ; ENSMUSP00000113257 ; ENSMUSG00000008540 .
GeneIDi 56615.
KEGGi mmu:56615.
UCSCi uc009enk.1. mouse.

Organism-specific databases

CTDi 4257.
MGIi MGI:1913850. Mgst1.

Phylogenomic databases

eggNOGi NOG71159.
GeneTreei ENSGT00390000011980.
HOGENOMi HOG000231759.
HOVERGENi HBG052470.
InParanoidi Q91VS7.
KOi K00799.
OrthoDBi EOG7288T0.
PhylomeDBi Q91VS7.
TreeFami TF105327.

Enzyme and pathway databases

Reactomei REACT_215316. Glutathione conjugation.
REACT_237136. Aflatoxin activation and detoxification.

Miscellaneous databases

ChiTaRSi Mgst1. mouse.
NextBioi 313039.
PROi Q91VS7.
SOURCEi Search...

Gene expression databases

Bgeei Q91VS7.
CleanExi MM_MGST1.
ExpressionAtlasi Q91VS7. baseline and differential.
Genevestigatori Q91VS7.

Family and domain databases

Gene3Di 1.20.120.550. 1 hit.
InterProi IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view ]
Pfami PF01124. MAPEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization and bacterial expression of full length cDNA for the mouse liver microsomal glutathione S-transferase."
    Raza H., Mullick J., Avadhani N.G.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum and Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-42; LYS-55 AND LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMGST1_MOUSE
AccessioniPrimary (citable) accession number: Q91VS7
Secondary accession number(s): Q9CQ57, Q9R191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3