Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein 24

Gene

Znf24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for myelination of differentiated oligodendrocytes. Required for the conversion of oligodendrocytes from the premyelinating to the myelinating state. In the developing central nervous system (CNS), involved in the maintenance in the progenitor stage by promoting the cell cycle. Specifically binds to the 5'-TCAT-3' DNA sequence. Has transcription repressor activity in vitro.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri251 – 27323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri279 – 30123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri307 – 32923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri335 – 35723C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. sequence-specific DNA binding Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. myelination Source: UniProtKB
  2. negative regulation of transcription, DNA-templated Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 24
Alternative name(s):
Hypomyelinated CNS protein
Zinc finger protein 191
Short name:
Zfp-191
Zinc finger protein ZF-12
Gene namesi
Name:Znf24
Synonyms:Hmcns, Zfp191
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1929704. Zfp191.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleoplasm Source: MGI
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

According to PubMed:20080941, most mice die by postnatal day 25 (P25) due to myelination defects. Brains and spinal cords isolated from P14 mice appear to be smaller than those from wild-type mice and to lack white matter. Mutant oligodendrocytes arrest at a late stage of differentiation. Expression of genes expressed specifically in mature myelinating oligodendrocytes is down-regulated. According to PubMed:17064688, embryos are severely retarded in development and die at approximately 7.5 days post-fertilization (E7.5). One possible explanation for the different phenotypes described is that the two null alleles are not identical. In the mutants described by PubMed:20080941, the PGK-neo cassette used for positive selection of embryonic stem (ES) cells has been removed, whereas it remains in the allele described by PubMed:17064688.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Zinc finger protein 24PRO_0000047353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei132 – 1321PhosphoserineBy similarity
Modified residuei142 – 1421PhosphoserineBy similarity
Modified residuei274 – 2741PhosphoserineBy similarity
Modified residuei335 – 3351PhosphotyrosineBy similarity

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ91VN1.
PaxDbiQ91VN1.
PRIDEiQ91VN1.

PTM databases

PhosphoSiteiQ91VN1.

Expressioni

Tissue specificityi

Widely expressed with highest levels in heart, brain, liver, skeletal muscle, kidney and testis and very low levels in spleen and lung.2 Publications

Developmental stagei

In the embryo, barely detectable at day 17, peaks at day 11 and remains constant thereafter. Detected early during embryogenesis in ectodermal, endodermal, mesodermal and extraembryonic tissues. Expressed in the developing central nervous system. In the developing central nervous system (CNS), mainly expressed in progenitors.2 Publications

Gene expression databases

BgeeiQ91VN1.
CleanExiMM_ZFP191.
ExpressionAtlasiQ91VN1. baseline and differential.
GenevestigatoriQ91VN1.

Interactioni

Protein-protein interaction databases

BioGridi208519. 1 interaction.
IntActiQ91VN1. 1 interaction.
STRINGi10090.ENSMUSP00000064637.

Structurei

3D structure databases

ProteinModelPortaliQ91VN1.
SMRiQ91VN1. Positions 46-136, 248-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 13483SCAN boxPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 30151Necessary and sufficient for nuclear localizationBy similarityAdd
BLAST

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri251 – 27323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri279 – 30123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri307 – 32923C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri335 – 35723C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118881.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ91VN1.
KOiK09230.
OMAiILIIPNP.
OrthoDBiEOG7HF1JG.
PhylomeDBiQ91VN1.
TreeFamiTF338304.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR008916. Retrov_capsid_C.
IPR003309. Tscrpt_reg_SCAN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02023. SCAN. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQSVEEDS ILIIPNPDEE EKILRVKLEE DPDGEEGSSI SWNHLPDPEV
60 70 80 90 100
FRQRFRQFGY QDSPGPREAV SQLRELCRLW LRPETHTKEQ ILELVVLEQF
110 120 130 140 150
VAILPKELQT WVREHHPENG EEAVAVLEDL ESELDDPGQP VSLRRQKREV
160 170 180 190 200
LVEEITSQED AQGLPSSELD AVENQLKWAS WELHSLRHCD DDATTENGAL
210 220 230 240 250
APKQEMASAG ESHEGPGTLN IGVPQLFKYG ETCFPKGRFE RKRNPSRKKQ
260 270 280 290 300
HICDECGKHF SQGSALILHQ RIHSGEKPYG CVECGKAFSR SSILVQHQRV
310 320 330 340 350
HTGEKPYKCL ECGKAFSQNS GLINHQRIHT GEKPYECVQC GKSYSQSSNL
360
FRHQRRHNAE KLLNVVKV
Length:368
Mass (Da):41,919
Last modified:December 1, 2001 - v1
Checksum:i344974009D2A5469
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911I → M in AAD37421 (PubMed:10542327).Curated
Sequence conflicti366 – 3661Missing in AAD37421 (PubMed:10542327).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149093 mRNA. Translation: AAD37421.1.
AY052495 Genomic DNA. Translation: AAL13429.1.
AY832930 mRNA. Translation: AAV91152.1.
AK146726 mRNA. Translation: BAE27389.1.
CH466557 Genomic DNA. Translation: EDK96990.1.
CH466557 Genomic DNA. Translation: EDK96991.1.
BC011345 mRNA. Translation: AAH11345.1.
BC054832 mRNA. Translation: AAH54832.1.
CCDSiCCDS29098.1.
RefSeqiNP_067534.2. NM_021559.2.
XP_006526199.1. XM_006526136.2.
XP_006526200.1. XM_006526137.2.
UniGeneiMm.417427.

Genome annotation databases

EnsembliENSMUST00000066497; ENSMUSP00000064637; ENSMUSG00000051469.
ENSMUST00000153337; ENSMUSP00000122579; ENSMUSG00000051469.
GeneIDi59057.
KEGGimmu:59057.
UCSCiuc008egm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149093 mRNA. Translation: AAD37421.1.
AY052495 Genomic DNA. Translation: AAL13429.1.
AY832930 mRNA. Translation: AAV91152.1.
AK146726 mRNA. Translation: BAE27389.1.
CH466557 Genomic DNA. Translation: EDK96990.1.
CH466557 Genomic DNA. Translation: EDK96991.1.
BC011345 mRNA. Translation: AAH11345.1.
BC054832 mRNA. Translation: AAH54832.1.
CCDSiCCDS29098.1.
RefSeqiNP_067534.2. NM_021559.2.
XP_006526199.1. XM_006526136.2.
XP_006526200.1. XM_006526137.2.
UniGeneiMm.417427.

3D structure databases

ProteinModelPortaliQ91VN1.
SMRiQ91VN1. Positions 46-136, 248-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208519. 1 interaction.
IntActiQ91VN1. 1 interaction.
STRINGi10090.ENSMUSP00000064637.

PTM databases

PhosphoSiteiQ91VN1.

Proteomic databases

MaxQBiQ91VN1.
PaxDbiQ91VN1.
PRIDEiQ91VN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066497; ENSMUSP00000064637; ENSMUSG00000051469.
ENSMUST00000153337; ENSMUSP00000122579; ENSMUSG00000051469.
GeneIDi59057.
KEGGimmu:59057.
UCSCiuc008egm.1. mouse.

Organism-specific databases

CTDi59057.
MGIiMGI:1929704. Zfp191.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118881.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ91VN1.
KOiK09230.
OMAiILIIPNP.
OrthoDBiEOG7HF1JG.
PhylomeDBiQ91VN1.
TreeFamiTF338304.

Miscellaneous databases

NextBioi314706.
PROiQ91VN1.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VN1.
CleanExiMM_ZFP191.
ExpressionAtlasiQ91VN1. baseline and differential.
GenevestigatoriQ91VN1.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR008916. Retrov_capsid_C.
IPR003309. Tscrpt_reg_SCAN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02023. SCAN. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, cloning, and expression of a new murine zinc finger encoding gene."
    Prost J.-P., Negre D., Cornet-Javaux F., Cortay J.-C., Cozzone A.J., Herbage D., Mallein-Gerin F.
    Biochim. Biophys. Acta 1447:278-283(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: CD-1.
    Tissue: Cartilage chondrocyte.
  2. "Cloning, genomic organization and promoter activity of the mouse zinc finger protein gene ZF-12."
    Li J.-Z., Chen X., Wang S.L., Sun X., Zhang Y.Z., Yu L., Fu J.-L.
    Yi Chuan Xue Bao 30:311-316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland and Retina.
  6. "The zinc finger transcription factor 191 is required for early embryonic development and cell proliferation."
    Li J., Chen X., Yang H., Wang S., Guo B., Yu L., Wang Z., Fu J.
    Exp. Cell Res. 312:3990-3998(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191."
    Wang H., Sun R., Liu G., Yao M., Fei J., Shen H.
    Acta Biochim. Biophys. Sin. 40:704-710(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  8. "Expression of the transcription factor Zfp191 during embryonic development in the mouse."
    Khalfallah O., Faucon-Biguet N., Nardelli J., Meloni R., Mallet J.
    Gene Expr. Patterns 8:148-154(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  9. "Zinc finger protein 191 (ZNF191/Zfp191) is necessary to maintain neural cells as cycling progenitors."
    Khalfallah O., Ravassard P., Lagache C.S., Fligny C., Serre A., Bayard E., Faucon-Biguet N., Mallet J., Meloni R., Nardelli J.
    Stem Cells 27:1643-1653(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiZNF24_MOUSE
AccessioniPrimary (citable) accession number: Q91VN1
Secondary accession number(s): Q5MDG8, Q9WUQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.