Reviewed, UniProtKB/Swiss-Prot Q91VM9 (IPYR2_MOUSE)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase 2, mitochondrial
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase 2
      Short name=PPase 2

Gene names

Name:

Ppa2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91VM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91VM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.
     127-170: IFPHKGYIWN...DPIDVCEIGS → MLDADAPQNC...FQAIETLSQM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 330	303	Inorganic pyrophosphatase 2, mitochondrial		PRO_0000025412
Sites
	Metal binding	159	1	Magnesium 1 By similarity
	Metal binding	164	1	Magnesium 1 By similarity
	Metal binding	164	1	Magnesium 2 By similarity
	Metal binding	196	1	Magnesium 1 By similarity
Amino acid modifications
	Modified residue	48	1	N6-acetyllysine By similarity
	Modified residue	211	1	N6-acetyllysine By similarity
	Modified residue	256	1	N6-acetyllysine By similarity
	Modified residue	271	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 126	126	Missing in isoform 2.		VSP_011653
	Alternative sequence	127 – 170	44	IFPHK…CEIGS → MLDADAPQNCQSWEGKDKQI PGPHRPNSQACLVKFQAIET LSQM in isoform 2.		VSP_011654
Experimental info
	Sequence conflict	225 – 227	3	YLE → HLQ in BAC41194. Ref.1
	Sequence conflict	254	1	K → T in BAC41194. Ref.1
	Sequence conflict	321	1	Missing in BAC41194. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 8AFD61FA561D7268	FASTA	330	38,115
	10 20 30 40 50 60 MRALLPLLSV GRGWRVGAAA RPPRRVMSLY RTEELGHPRS QDYRLFFKHV AGHYISPFHD 70 80 90 100 110 120 IPLKADCKEE HDIPRKKARN DEYENLFNMV VEIPRWTNAK MEIATEEPLN PIKQDIKNGK 130 140 150 160 170 180 LRYTPNIFPH KGYIWNYGAL PQTWEDPHLR DKSTDCCGDN DPIDVCEIGS KVLSRGDVVH 190 200 210 220 230 240 VKILGTLALI DQSETDWKII AINVNDPEAE KFHDIDDVKK FKPGYLEATL NWFRLYKVPD 250 260 270 280 290 300 GKPENKFAFN GEFKNKAFAL DVINSAHERW KEMVMKKCDK GAISCVNVHI CDSPFHCTME 310 320 330 EARSLVESVP TPSMNKESNV EEEVWHFLRN
	Isoform 2. Checksum: 27E5DA9D3A368CC9	FASTA	204	23,274
	10 20 30 40 50 60 MLDADAPQNC QSWEGKDKQI PGPHRPNSQA CLVKFQAIET LSQMKVLSRG DVVHVKILGT 70 80 90 100 110 120 LALIDQSETD WKIIAINVND PEAEKFHDID DVKKFKPGYL EATLNWFRLY KVPDGKPENK 130 140 150 160 170 180 FAFNGEFKNK AFALDVINSA HERWKEMVMK KCDKGAISCV NVHICDSPFH CTMEEARSLV 190 200 ESVPTPSMNK ESNVEEEVWH FLRN

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Embryo and Spinal cord.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: NMRI. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK003660 mRNA. Translation: BAB22922.1. AK090384 mRNA. Translation: BAC41194.1. AK143475 mRNA. Translation: BAE25392.1. BC011417 mRNA. Translation: AAH11417.1.
IPI	IPI00127050. IPI00408727.
RefSeq	NP_666253.1.
UniGene	Mm.210305
3D structure databases
SMR	Q91VM9. Positions 30-328.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91VM9.
PTM databases
PhosphoSite	Q91VM9.
2-D gel databases
REPRODUCTION-2DPAGE	Q91VM9.
Proteomic databases
PRIDE	Q91VM9.
Genome annotation databases
Ensembl	ENSMUST00000029644; ENSMUSP00000029644; ENSMUSG00000028013; Mus musculus. [Genome view] ENSMUST00000106315; ENSMUSP00000101922; ENSMUSG00000028013; Mus musculus. [Genome view]
GeneID	74776.
KEGG	mmu:74776.
UCSC	uc008rkm.1. mouse.
Organism-specific databases
CTD	74776.
MGI	MGI:1922026. Ppa2.
Phylogenomic databases
eggNOG	roNOG04947.
HOGENOM	HBG434750.
HOVERGEN	Q91VM9.
InParanoid	Q91VM9.
OMA	WFFISGS.
PhylomeDB	Q91VM9.
Enzyme and pathway databases
BRENDA	3.6.1.1. 244.
Gene expression databases
ArrayExpress	Q91VM9.
Bgee	Q91VM9.
CleanEx	MM_PPA2.
Genevestigator	Q91VM9.
GermOnline	ENSMUSG00000028013. Mus musculus.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	341614.
SOURCE	Search...

Entry information

Entry name

IPYR2_MOUSE

Accession

Primary (citable) accession number: Q91VM9
Secondary accession number(s): Q3UPK3, Q8BTG5, Q9D1E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)