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Protein

Inorganic pyrophosphatase 2, mitochondrial

Gene

Ppa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi159 – 1591Magnesium 1By similarity
Metal bindingi164 – 1641Magnesium 1By similarity
Metal bindingi164 – 1641Magnesium 2By similarity
Metal bindingi196 – 1961Magnesium 1By similarity

GO - Molecular functioni

GO - Biological processi

  • protein dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-379726. Mitochondrial tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase 2, mitochondrial (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate phospho-hydrolase 2
Short name:
PPase 2
Gene namesi
Name:Ppa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1922026. Ppa2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 330303Inorganic pyrophosphatase 2, mitochondrialPRO_0000025412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111N6-succinyllysineCombined sources
Modified residuei219 – 2191N6-acetyllysineCombined sources
Modified residuei254 – 2541N6-succinyllysineCombined sources
Modified residuei256 – 2561N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91VM9.
MaxQBiQ91VM9.
PaxDbiQ91VM9.
PRIDEiQ91VM9.

2D gel databases

REPRODUCTION-2DPAGEQ91VM9.

PTM databases

iPTMnetiQ91VM9.
PhosphoSiteiQ91VM9.
SwissPalmiQ91VM9.

Expressioni

Gene expression databases

BgeeiQ91VM9.
CleanExiMM_PPA2.
ExpressionAtlasiQ91VM9. baseline and differential.
GenevisibleiQ91VM9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi217012. 1 interaction.
IntActiQ91VM9. 3 interactions.
MINTiMINT-1843615.
STRINGi10090.ENSMUSP00000029644.

Structurei

3D structure databases

ProteinModelPortaliQ91VM9.
SMRiQ91VM9. Positions 34-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1626. Eukaryota.
COG0221. LUCA.
GeneTreeiENSGT00390000017004.
HOGENOMiHOG000195569.
HOVERGENiHBG000491.
InParanoidiQ91VM9.
KOiK01507.
OMAiENVAGHY.
OrthoDBiEOG7R2BKH.
PhylomeDBiQ91VM9.
TreeFamiTF300887.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRALLPLLSV GRGWRVGAAA RPPRRVMSLY RTEELGHPRS QDYRLFFKHV
60 70 80 90 100
AGHYISPFHD IPLKADCKEE HDIPRKKARN DEYENLFNMV VEIPRWTNAK
110 120 130 140 150
MEIATEEPLN PIKQDIKNGK LRYTPNIFPH KGYIWNYGAL PQTWEDPHLR
160 170 180 190 200
DKSTDCCGDN DPIDVCEIGS KVLSRGDVVH VKILGTLALI DQSETDWKII
210 220 230 240 250
AINVNDPEAE KFHDIDDVKK FKPGYLEATL NWFRLYKVPD GKPENKFAFN
260 270 280 290 300
GEFKNKAFAL DVINSAHERW KEMVMKKCDK GAISCVNVHI CDSPFHCTME
310 320 330
EARSLVESVP TPSMNKESNV EEEVWHFLRN
Length:330
Mass (Da):38,115
Last modified:December 1, 2001 - v1
Checksum:i8AFD61FA561D7268
GO
Isoform 2 (identifier: Q91VM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.
     127-170: IFPHKGYIWN...DPIDVCEIGS → MLDADAPQNC...FQAIETLSQM

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):23,274
Checksum:i27E5DA9D3A368CC9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2273YLE → HLQ in BAC41194 (PubMed:16141072).Curated
Sequence conflicti254 – 2541K → T in BAC41194 (PubMed:16141072).Curated
Sequence conflicti321 – 3211Missing in BAC41194 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 126126Missing in isoform 2. 1 PublicationVSP_011653Add
BLAST
Alternative sequencei127 – 17044IFPHK…CEIGS → MLDADAPQNCQSWEGKDKQI PGPHRPNSQACLVKFQAIET LSQM in isoform 2. 1 PublicationVSP_011654Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003660 mRNA. Translation: BAB22922.1.
AK090384 mRNA. Translation: BAC41194.1.
AK143475 mRNA. Translation: BAE25392.1.
BC011417 mRNA. Translation: AAH11417.1.
CCDSiCCDS17850.1. [Q91VM9-1]
RefSeqiNP_001280570.1. NM_001293641.1.
NP_666253.1. NM_146141.2. [Q91VM9-1]
UniGeneiMm.210305.

Genome annotation databases

EnsembliENSMUST00000029644; ENSMUSP00000029644; ENSMUSG00000028013. [Q91VM9-1]
GeneIDi74776.
KEGGimmu:74776.
UCSCiuc008rkm.2. mouse. [Q91VM9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003660 mRNA. Translation: BAB22922.1.
AK090384 mRNA. Translation: BAC41194.1.
AK143475 mRNA. Translation: BAE25392.1.
BC011417 mRNA. Translation: AAH11417.1.
CCDSiCCDS17850.1. [Q91VM9-1]
RefSeqiNP_001280570.1. NM_001293641.1.
NP_666253.1. NM_146141.2. [Q91VM9-1]
UniGeneiMm.210305.

3D structure databases

ProteinModelPortaliQ91VM9.
SMRiQ91VM9. Positions 34-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217012. 1 interaction.
IntActiQ91VM9. 3 interactions.
MINTiMINT-1843615.
STRINGi10090.ENSMUSP00000029644.

PTM databases

iPTMnetiQ91VM9.
PhosphoSiteiQ91VM9.
SwissPalmiQ91VM9.

2D gel databases

REPRODUCTION-2DPAGEQ91VM9.

Proteomic databases

EPDiQ91VM9.
MaxQBiQ91VM9.
PaxDbiQ91VM9.
PRIDEiQ91VM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029644; ENSMUSP00000029644; ENSMUSG00000028013. [Q91VM9-1]
GeneIDi74776.
KEGGimmu:74776.
UCSCiuc008rkm.2. mouse. [Q91VM9-1]

Organism-specific databases

CTDi27068.
MGIiMGI:1922026. Ppa2.

Phylogenomic databases

eggNOGiKOG1626. Eukaryota.
COG0221. LUCA.
GeneTreeiENSGT00390000017004.
HOGENOMiHOG000195569.
HOVERGENiHBG000491.
InParanoidiQ91VM9.
KOiK01507.
OMAiENVAGHY.
OrthoDBiEOG7R2BKH.
PhylomeDBiQ91VM9.
TreeFamiTF300887.

Enzyme and pathway databases

ReactomeiR-MMU-379726. Mitochondrial tRNA aminoacylation.

Miscellaneous databases

ChiTaRSiPpa2. mouse.
PROiQ91VM9.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VM9.
CleanExiMM_PPA2.
ExpressionAtlasiQ91VM9. baseline and differential.
GenevisibleiQ91VM9. MM.

Family and domain databases

Gene3Di3.90.80.10. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Spinal cord.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: NMRI.
    Tissue: Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-211 AND LYS-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-219 AND LYS-256, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIPYR2_MOUSE
AccessioniPrimary (citable) accession number: Q91VM9
Secondary accession number(s): Q3UPK3, Q8BTG5, Q9D1E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.