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Protein

Protein spinster homolog 2

Gene

Spns2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration (By similarity).By similarity

GO - Molecular functioni

  • sphingolipid transporter activity Source: MGI

GO - Biological processi

  • B cell homeostasis Source: MGI
  • bone development Source: MGI
  • lymph node development Source: MGI
  • lymphocyte homeostasis Source: MGI
  • lymphocyte migration Source: MGI
  • regulation of eye pigmentation Source: MGI
  • regulation of humoral immune response Source: MGI
  • sphingolipid metabolic process Source: MGI
  • sphingosine-1-phosphate signaling pathway Source: MGI
  • T cell homeostasis Source: MGI
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Chemistry

SwissLipidsiSLP:000001139.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spinster homolog 2
Gene namesi
Name:Spns2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2384936. Spns2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence analysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Transmembranei320 – 34021HelicalSequence analysisAdd
BLAST
Transmembranei364 – 38421HelicalSequence analysisAdd
BLAST
Transmembranei398 – 41821HelicalSequence analysisAdd
BLAST
Transmembranei422 – 44221HelicalSequence analysisAdd
BLAST
Transmembranei466 – 48621HelicalSequence analysisAdd
BLAST
Transmembranei507 – 52721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Protein spinster homolog 2PRO_0000305044Add
BLAST

Proteomic databases

MaxQBiQ91VM4.
PaxDbiQ91VM4.
PRIDEiQ91VM4.

PTM databases

iPTMnetiQ91VM4.
PhosphoSiteiQ91VM4.

Expressioni

Gene expression databases

BgeeiQ91VM4.
CleanExiMM_SPNS2.
ExpressionAtlasiQ91VM4. baseline and differential.
GenevisibleiQ91VM4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044418.

Structurei

3D structure databases

ProteinModelPortaliQ91VM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1330. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00390000005976.
HOGENOMiHOG000276167.
HOVERGENiHBG055503.
InParanoidiQ91VM4.
OMAiTLLFVNW.
OrthoDBiEOG783MVC.
TreeFamiTF314395.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VM4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMCLECASAA AGGAEEEEAD AERRRRRRGA QPGAGGSACC GARGVGGAGV
60 70 80 90 100
VSADEEVQTL SGSVRRVPSG LPSIPSTPGC AAAAKGPSAP QPKPASLGRG
110 120 130 140 150
RGAAAAILSL GNVLNYLDRY TVAGVLLDIQ QHFGVKDRGA GLLQSVFICS
160 170 180 190 200
FMVAAPIFGY LGDRFNRKVI LSCGIFFWSA VTFSSSFIPQ QYFWLLVLSR
210 220 230 240 250
GLVGIGEASY STIAPTIIGD LFTKNTRTLM LSVFYFAIPL GSGLGYITGS
260 270 280 290 300
SVKQAAGDWH WALRVSPVLG MITGTLILIL VPATKRGHAD QLGGQLKART
310 320 330 340 350
SWLRDMKALI RNRSYVFSSL ATSAVSFATG ALGMWIPLYL HRAQVVQKTA
360 370 380 390 400
ETCNSPPCGA KDSLIFGAIT CFTGFLGVVT GAGATRWCRL RTQRADPLVC
410 420 430 440 450
AVGMLGSAIF ICLIFVAAKT SIVGAYICIF VGETLLFSNW AITADILMYV
460 470 480 490 500
VIPTRRATAV ALQSFTSHLL GDAGSPYLIG FISDLIRQST KDSPLWEFLS
510 520 530 540
LGYALMLCPF VVVLGGMFFL ATALFFLSDR AKAEQQVNQL VMPPASVKV
Length:549
Mass (Da):58,196
Last modified:October 2, 2007 - v2
Checksum:iBE82D3254BA8C8C3
GO
Isoform 2 (identifier: Q91VM4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-431: Missing.
     432-448: GETLLFSNWAITADILM → MSLVHGSSSPDLPFLLQ

Note: No experimental confirmation available.
Show »
Length:118
Mass (Da):12,864
Checksum:i205DB05F92429659
GO

Sequence cautioni

The sequence AAH11467.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 431431Missing in isoform 2. 1 PublicationVSP_036390Add
BLAST
Alternative sequencei432 – 44817GETLL…ADILM → MSLVHGSSSPDLPFLLQ in isoform 2. 1 PublicationVSP_036391Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL662812 Genomic DNA. Translation: CAI52006.1.
BC011467 mRNA. Translation: AAH11467.1. Different initiation.
BC025823 mRNA. Translation: AAH25823.1.
AB441166 mRNA. Translation: BAH15193.1.
CCDSiCCDS48841.1. [Q91VM4-1]
RefSeqiNP_694700.2. NM_153060.3. [Q91VM4-1]
UniGeneiMm.212812.

Genome annotation databases

EnsembliENSMUST00000045303; ENSMUSP00000044418; ENSMUSG00000040447. [Q91VM4-1]
GeneIDi216892.
KEGGimmu:216892.
UCSCiuc007jyz.3. mouse. [Q91VM4-1]
uc007jza.1. mouse. [Q91VM4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL662812 Genomic DNA. Translation: CAI52006.1.
BC011467 mRNA. Translation: AAH11467.1. Different initiation.
BC025823 mRNA. Translation: AAH25823.1.
AB441166 mRNA. Translation: BAH15193.1.
CCDSiCCDS48841.1. [Q91VM4-1]
RefSeqiNP_694700.2. NM_153060.3. [Q91VM4-1]
UniGeneiMm.212812.

3D structure databases

ProteinModelPortaliQ91VM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044418.

Chemistry

SwissLipidsiSLP:000001139.

PTM databases

iPTMnetiQ91VM4.
PhosphoSiteiQ91VM4.

Proteomic databases

MaxQBiQ91VM4.
PaxDbiQ91VM4.
PRIDEiQ91VM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045303; ENSMUSP00000044418; ENSMUSG00000040447. [Q91VM4-1]
GeneIDi216892.
KEGGimmu:216892.
UCSCiuc007jyz.3. mouse. [Q91VM4-1]
uc007jza.1. mouse. [Q91VM4-2]

Organism-specific databases

CTDi124976.
MGIiMGI:2384936. Spns2.

Phylogenomic databases

eggNOGiKOG1330. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00390000005976.
HOGENOMiHOG000276167.
HOVERGENiHBG055503.
InParanoidiQ91VM4.
OMAiTLLFVNW.
OrthoDBiEOG783MVC.
TreeFamiTF314395.

Miscellaneous databases

ChiTaRSiSpns2. mouse.
PROiQ91VM4.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VM4.
CleanExiMM_SPNS2.
ExpressionAtlasiQ91VM4. baseline and differential.
GenevisibleiQ91VM4. MM.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: 129 and FVB/N.
    Tissue: Liver and Mammary tumor.
  3. "The sphingolipid transporter spns2 functions in migration of zebrafish myocardial precursors."
    Kawahara A., Nishi T., Hisano Y., Fukui H., Yamaguchi A., Mochizuki N.
    Science 323:524-527(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-549 (ISOFORM 1).

Entry informationi

Entry nameiSPNS2_MOUSE
AccessioniPrimary (citable) accession number: Q91VM4
Secondary accession number(s): B9A1T4, Q5F242, Q8R119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: June 8, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.