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Protein

Polyglutamine-binding protein 1

Gene

Pqbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions as scaffold protein that is part of numerous complexes and thereby plays a role in pre-mRNA splicing, transcription regulation and neuron development. May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit (By similarity). Required for normal alternative splicing of target pre-mRNA species (PubMed:23512658).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • regulation of dendrite morphogenesis Source: MGI
  • regulation of RNA splicing Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • stress granule assembly Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyglutamine-binding protein 1
Short name:
PQBP-1
Alternative name(s):
38 kDa nuclear protein containing a WW domain
Short name:
Npw38
Polyglutamine tract-binding protein 1
Gene namesi
Name:Pqbp1
Synonyms:Npw38
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1859638. Pqbp1.

Subcellular locationi

  • Nucleus 1 Publication
  • Nucleus speckle 1 Publication

  • Note: Colocalized with ATXN1 in nuclear inclusion bodies. Colocalized with POU3F2 (By similarity). Colocalizes with SRSF2 in nuclear speckles.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic stress granule Source: MGI
  • neuronal ribonucleoprotein granule Source: MGI
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000760902 – 263Polyglutamine-binding protein 1Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei245PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91VJ5.
MaxQBiQ91VJ5.
PaxDbiQ91VJ5.
PRIDEiQ91VJ5.

PTM databases

iPTMnetiQ91VJ5.
PhosphoSitePlusiQ91VJ5.

Expressioni

Tissue specificityi

Detected in brain cortex and hippocampus neurons (at protein level). Expressed in brain with high level in cerebellar cortex, hippocampus and olfactory bulb.2 Publications

Gene expression databases

BgeeiENSMUSG00000031157.
CleanExiMM_PQBP1.
ExpressionAtlasiQ91VJ5. baseline and differential.
GenevisibleiQ91VJ5. MM.

Interactioni

Subunit structurei

Interacts with POU3F2/Brn-2, ATXN1, TXNL4A, HTT and AR. Interaction with ATXN1 correlates positively with the length of the polyglutamine tract. Interacts with RNA polymerase II large subunit in a phosphorylation-dependent manner. Forms a ternary complex with ATXN1 mutant and phosphorylated RNA polymerase II. Interacts (via C-terminus) with TXNL4A and CD2BP2. Interacts (via WW domain) with ATN1, WBP11 and SF3B1, and may interact with additional splice factors (By similarity).By similarity

Protein-protein interaction databases

BioGridi207696. 24 interactors.
IntActiQ91VJ5. 24 interactors.
STRINGi10090.ENSMUSP00000033497.

Structurei

3D structure databases

ProteinModelPortaliQ91VJ5.
SMRiQ91VJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 80WWPROSITE-ProRule annotationAdd BLAST35
Repeati104 – 1101-1; approximate7
Repeati111 – 1171-27
Repeati118 – 1241-3; approximate7
Repeati125 – 1311-4; approximate7
Repeati132 – 1381-5; approximate7
Repeati139 – 1402-12
Repeati141 – 1422-22
Repeati143 – 1442-32
Repeati150 – 1513-12
Repeati152 – 1533-22
Repeati154 – 1553-32
Repeati156 – 1573-42
Repeati158 – 1593-52
Repeati160 – 1613-62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 263Intrinsically disorderedBy similarityAdd BLAST170
Regioni104 – 1385 X 7 AA approximate tandem repeats of D-R-[NS]-H-E-K-SAdd BLAST35
Regioni139 – 1443 X 2 AA tandem repeats of [DE]-R6
Regioni150 – 1616 X 2 AA tandem repeats of [DE]-RAdd BLAST12
Regioni243 – 253Important for interaction with TXNL4ABy similarityAdd BLAST11

Domaini

The WW domain may play a role as a transcriptional activator directly or via association with the transcription machinery. The WW domain mediates interaction with WBP11, ATN1, SF3B1 and the C-terminal domain of the RNA polymerase II large subunit (By similarity).By similarity
Except for the WW domain, the protein is intrinsically disordered.By similarity

Sequence similaritiesi

Contains 1 WW domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3427. Eukaryota.
ENOG4111MHA. LUCA.
GeneTreeiENSGT00390000001905.
HOGENOMiHOG000231359.
HOVERGENiHBG053064.
InParanoidiQ91VJ5.
KOiK12865.
OMAiKSDRSHD.
OrthoDBiEOG091G0H7P.
PhylomeDBiQ91VJ5.
TreeFamiTF320689.

Family and domain databases

InterProiIPR001202. WW_dom.
[Graphical view]
PfamiPF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiPS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPVALQTR LAKRGILKHL EPEPEEEIIA EDYDDDPVDY EATRIEGLPP
60 70 80 90 100
SWYKVFDPSC GLPYYWNVET DLVSWLSPHD PNFVVTKSAK KVRNNNADAE
110 120 130 140 150
DKSDRNLEKV DRNHEKSDRS HEKPDRSHEK ADRNHEKNDR ERERNYDKVD
160 170 180 190 200
RERDRDRERE RAFDKADREE GKDRRHHRRE ELAPYPKNKK ATSRKDEELD
210 220 230 240 250
PMDPSSYSDA PRGTWSTGLP KRNEAKTGAD TTAAGPLFQQ RPYPSPGAVL
260
RANAEASRTK QQD
Length:263
Mass (Da):30,597
Last modified:December 1, 2001 - v1
Checksum:iF62304963D34D22B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → S in AAH51673 (PubMed:15489334).Curated1
Sequence conflicti177H → L in CAB59205 (Ref. 1) Curated1
Sequence conflicti177H → L in CAC15062 (Ref. 1) Curated1
Sequence conflicti183A → V in CAB59205 (Ref. 1) Curated1
Sequence conflicti183A → V in CAC15062 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250406 mRNA. Translation: CAB59205.1.
AJ296289 Genomic DNA. Translation: CAC15062.1.
AK077652 mRNA. Translation: BAC36928.1.
BC009657 mRNA. Translation: AAH09657.1.
BC051673 mRNA. Translation: AAH51673.1.
CCDSiCCDS29977.1.
RefSeqiNP_001239457.1. NM_001252528.1.
NP_001239458.1. NM_001252529.1.
NP_062351.2. NM_019478.4.
XP_006527717.1. XM_006527654.1.
UniGeneiMm.14616.

Genome annotation databases

EnsembliENSMUST00000033497; ENSMUSP00000033497; ENSMUSG00000031157.
ENSMUST00000115654; ENSMUSP00000111318; ENSMUSG00000031157.
ENSMUST00000115655; ENSMUSP00000111319; ENSMUSG00000031157.
GeneIDi54633.
KEGGimmu:54633.
UCSCiuc009snd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250406 mRNA. Translation: CAB59205.1.
AJ296289 Genomic DNA. Translation: CAC15062.1.
AK077652 mRNA. Translation: BAC36928.1.
BC009657 mRNA. Translation: AAH09657.1.
BC051673 mRNA. Translation: AAH51673.1.
CCDSiCCDS29977.1.
RefSeqiNP_001239457.1. NM_001252528.1.
NP_001239458.1. NM_001252529.1.
NP_062351.2. NM_019478.4.
XP_006527717.1. XM_006527654.1.
UniGeneiMm.14616.

3D structure databases

ProteinModelPortaliQ91VJ5.
SMRiQ91VJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207696. 24 interactors.
IntActiQ91VJ5. 24 interactors.
STRINGi10090.ENSMUSP00000033497.

PTM databases

iPTMnetiQ91VJ5.
PhosphoSitePlusiQ91VJ5.

Proteomic databases

EPDiQ91VJ5.
MaxQBiQ91VJ5.
PaxDbiQ91VJ5.
PRIDEiQ91VJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033497; ENSMUSP00000033497; ENSMUSG00000031157.
ENSMUST00000115654; ENSMUSP00000111318; ENSMUSG00000031157.
ENSMUST00000115655; ENSMUSP00000111319; ENSMUSG00000031157.
GeneIDi54633.
KEGGimmu:54633.
UCSCiuc009snd.2. mouse.

Organism-specific databases

CTDi10084.
MGIiMGI:1859638. Pqbp1.

Phylogenomic databases

eggNOGiKOG3427. Eukaryota.
ENOG4111MHA. LUCA.
GeneTreeiENSGT00390000001905.
HOGENOMiHOG000231359.
HOVERGENiHBG053064.
InParanoidiQ91VJ5.
KOiK12865.
OMAiKSDRSHD.
OrthoDBiEOG091G0H7P.
PhylomeDBiQ91VJ5.
TreeFamiTF320689.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ91VJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031157.
CleanExiMM_PQBP1.
ExpressionAtlasiQ91VJ5. baseline and differential.
GenevisibleiQ91VJ5. MM.

Family and domain databases

InterProiIPR001202. WW_dom.
[Graphical view]
PfamiPF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiPS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPQBP1_MOUSE
AccessioniPrimary (citable) accession number: Q91VJ5
Secondary accession number(s): Q80WW2, Q9ER43, Q9QYY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.