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Protein

Serine/threonine-protein kinase 38

Gene

Stk38

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Activated by binding of S100B which releases autoinhibitory N-lobe interactions, enabling ATP to bind and the autophosphorylation of Ser-281. Thr-444 then undergoes calcium-dependent phosphorylation by STK24/MST3. Interactions between phosphorylated Thr-444 and the N-lobe promote additional structural changes that complete the activation of the kinase. Autoinhibition is also released by the binding of MOB1/MOBKL1A and MOB2/HCCA2 to the N-terminal of STK38.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181ATPPROSITE-ProRule annotationBy similarity
Active sitei212 – 2121Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1039ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 38 (EC:2.7.11.1)
Alternative name(s):
NDR1 protein kinase
Nuclear Dbf2-related kinase 1
Gene namesi
Name:Stk38Imported
Synonyms:Ndr1By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2442572. Stk38.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 465464Serine/threonine-protein kinase 38PRO_0000086719Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei74 – 741PhosphothreonineBy similarity
Modified residuei264 – 2641PhosphoserineBy similarity
Modified residuei281 – 2811Phosphoserine; by autocatalysisBy similarity
Modified residuei444 – 4441Phosphothreonine; by STK24/MST3By similarity

Post-translational modificationi

ISGylated.By similarity
Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ91VJ4.
PaxDbiQ91VJ4.
PRIDEiQ91VJ4.

PTM databases

PhosphoSiteiQ91VJ4.

Expressioni

Tissue specificityi

Expressed at high levels in spleen, lung, thymus, brain and fat tissue.1 Publication

Gene expression databases

BgeeiQ91VJ4.
CleanExiMM_STK38.
ExpressionAtlasiQ91VJ4. baseline and differential.
GenevestigatoriQ91VJ4.

Interactioni

Subunit structurei

Homodimeric S100B binds two molecules of STK38. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with MOB1 and MOB2 (By similarity). Interacts with MAP3K1 and MAP3K2 (via the kinase catalytic domain) (By similarity). Forms a tripartite complex with MOBKL1B and STK3/MST2 (By similarity). Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mical1Q8VDP39EBI-2527046,EBI-4394891
STK4Q130432EBI-2527046,EBI-367376From a different organism.

Protein-protein interaction databases

BioGridi223068. 10 interactions.
IntActiQ91VJ4. 11 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ91VJ4.
SMRiQ91VJ4. Positions 19-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 382294Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini383 – 45573AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 8726Interaction with S100BBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
HOVERGENiHBG104247.
InParanoidiQ91VJ4.
KOiK08790.
OMAiMSTCRKR.
OrthoDBiEOG7BZVS1.
PhylomeDBiQ91VJ4.
TreeFamiTF105337.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMTGSTPCS SMSNHTKERV TMTKVTLENF YSNLIAQHEE REMRQKKLEK
60 70 80 90 100
VMEEEGLKDE EKRLRRSAHA RKETEFLRLK RTRLGLEDFE SLKVIGRGAF
110 120 130 140 150
GEVRLVQKKD TGHVYAMKIL RKADMLEKEQ VGHIRAERDI LVEADSLWVV
160 170 180 190 200
KMFYSFQDKL NLYLIMEFLP GGDMMTLLMK KDTLTEEETQ FYIAETVLAI
210 220 230 240 250
DSIHQLGFIH RDIKPDNLLL DSKGHVKLSD FGLCTGLKKA HRTEFYRNLN
260 270 280 290 300
HSLPSDFTFQ NMNSKRKAET WKRNRRQLAF STVGTPDYIA PEVFMQTGYN
310 320 330 340 350
KLCDWWSLGV IMYEMLIGYP PFCSETPQET YKKVMNWKET LTFPPEVPVS
360 370 380 390 400
EKAKGLILRF CCEWEHRIGA PGVEEIKNNL FFEGVDWEHI RERPAAISIE
410 420 430 440 450
IKSIDDTSNF DEFPESDILK PTVTTSSHPE TDYKNKDWVF INYTYKRFEG
460
LTARGAIPSY MKAAK
Length:465
Mass (Da):54,174
Last modified:December 1, 2001 - v1
Checksum:i00E29F8963196750
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY292399 mRNA. Translation: AAP44997.1.
BC009658 mRNA. Translation: AAH09658.1.
CCDSiCCDS28589.1.
RefSeqiNP_598876.1. NM_134115.2.
XP_006536793.1. XM_006536730.2.
UniGeneiMm.439986.

Genome annotation databases

EnsembliENSMUST00000009138; ENSMUSP00000009138; ENSMUSG00000024006.
ENSMUST00000119274; ENSMUSP00000113657; ENSMUSG00000024006.
GeneIDi106504.
KEGGimmu:106504.
UCSCiuc008bsa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY292399 mRNA. Translation: AAP44997.1.
BC009658 mRNA. Translation: AAH09658.1.
CCDSiCCDS28589.1.
RefSeqiNP_598876.1. NM_134115.2.
XP_006536793.1. XM_006536730.2.
UniGeneiMm.439986.

3D structure databases

ProteinModelPortaliQ91VJ4.
SMRiQ91VJ4. Positions 19-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223068. 10 interactions.
IntActiQ91VJ4. 11 interactions.

PTM databases

PhosphoSiteiQ91VJ4.

Proteomic databases

MaxQBiQ91VJ4.
PaxDbiQ91VJ4.
PRIDEiQ91VJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009138; ENSMUSP00000009138; ENSMUSG00000024006.
ENSMUST00000119274; ENSMUSP00000113657; ENSMUSG00000024006.
GeneIDi106504.
KEGGimmu:106504.
UCSCiuc008bsa.1. mouse.

Organism-specific databases

CTDi11329.
MGIiMGI:2442572. Stk38.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
HOVERGENiHBG104247.
InParanoidiQ91VJ4.
KOiK08790.
OMAiMSTCRKR.
OrthoDBiEOG7BZVS1.
PhylomeDBiQ91VJ4.
TreeFamiTF105337.

Miscellaneous databases

ChiTaRSiStk38. mouse.
NextBioi358230.
PROiQ91VJ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VJ4.
CleanExiMM_STK38.
ExpressionAtlasiQ91VJ4. baseline and differential.
GenevestigatoriQ91VJ4.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of NDR2 protein kinase by multi-site phosphorylation and the S100B calcium-binding protein."
    Stegert M.R., Tamaskovic R., Bichsel S.J., Hergovich A., Hemmings B.A.
    J. Biol. Chem. 279:23806-23812(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6Imported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.
  3. Cited for: FUNCTION, INTERACTION WITH MICAL1.

Entry informationi

Entry nameiSTK38_MOUSE
AccessioniPrimary (citable) accession number: Q91VJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.