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Q91VJ4

- STK38_MOUSE

UniProt

Q91VJ4 - STK38_MOUSE

Protein

Serine/threonine-protein kinase 38

Gene

Stk38

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated by binding of S100B which releases autoinhibitory N-lobe interactions, enabling ATP to bind and the autophosphorylation of Ser-281. Thr-444 then undergoes calcium-dependent phosphorylation by STK24/MST3. Interactions between phosphorylated Thr-444 and the N-lobe promote additional structural changes that complete the activation of the kinase. Autoinhibition is also released by the binding of MOB1/MOBKL1A and MOB2/HCCA2 to the N-terminal of STK38.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181ATPBy similarityPROSITE-ProRule annotation
    Active sitei212 – 2121Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi95 – 1039ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. negative regulation of MAP kinase activity Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 38 (EC:2.7.11.1)
    Alternative name(s):
    NDR1 protein kinase
    Nuclear Dbf2-related kinase 1
    Gene namesi
    Name:Stk38Imported
    Synonyms:Ndr1By similarity
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2442572. Stk38.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. MLL5-L complex Source: Ensembl
    3. nucleus Source: UniProtKB
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 465464Serine/threonine-protein kinase 38PRO_0000086719Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei74 – 741PhosphothreonineBy similarity
    Modified residuei264 – 2641PhosphoserineBy similarity
    Modified residuei281 – 2811Phosphoserine; by autocatalysisBy similarity
    Modified residuei444 – 4441Phosphothreonine; by STK24/MST3By similarity

    Post-translational modificationi

    ISGylated.By similarity
    Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ91VJ4.
    PaxDbiQ91VJ4.
    PRIDEiQ91VJ4.

    PTM databases

    PhosphoSiteiQ91VJ4.

    Expressioni

    Tissue specificityi

    Expressed at high levels in spleen, lung, thymus, brain and fat tissue.1 Publication

    Gene expression databases

    ArrayExpressiQ91VJ4.
    BgeeiQ91VJ4.
    CleanExiMM_STK38.
    GenevestigatoriQ91VJ4.

    Interactioni

    Subunit structurei

    Homodimeric S100B binds two molecules of STK38. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with MOB1 and MOB2 By similarity. Interacts with MAP3K1 and MAP3K2 (via the kinase catalytic domain) By similarity. Forms a tripartite complex with MOBKL1B and STK3/MST2 By similarity. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Mical1Q8VDP39EBI-2527046,EBI-4394891
    STK4Q130432EBI-2527046,EBI-367376From a different organism.

    Protein-protein interaction databases

    BioGridi223068. 10 interactions.
    IntActiQ91VJ4. 11 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91VJ4.
    SMRiQ91VJ4. Positions 23-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini89 – 382294Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini383 – 45573AGC-kinase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni62 – 8726Interaction with S100BBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117396.
    HOGENOMiHOG000233033.
    HOVERGENiHBG104247.
    InParanoidiQ91VJ4.
    KOiK08790.
    OMAiNAAYPPQ.
    OrthoDBiEOG7BZVS1.
    PhylomeDBiQ91VJ4.
    TreeFamiTF105337.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view]
    SMARTiSM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91VJ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMTGSTPCS SMSNHTKERV TMTKVTLENF YSNLIAQHEE REMRQKKLEK    50
    VMEEEGLKDE EKRLRRSAHA RKETEFLRLK RTRLGLEDFE SLKVIGRGAF 100
    GEVRLVQKKD TGHVYAMKIL RKADMLEKEQ VGHIRAERDI LVEADSLWVV 150
    KMFYSFQDKL NLYLIMEFLP GGDMMTLLMK KDTLTEEETQ FYIAETVLAI 200
    DSIHQLGFIH RDIKPDNLLL DSKGHVKLSD FGLCTGLKKA HRTEFYRNLN 250
    HSLPSDFTFQ NMNSKRKAET WKRNRRQLAF STVGTPDYIA PEVFMQTGYN 300
    KLCDWWSLGV IMYEMLIGYP PFCSETPQET YKKVMNWKET LTFPPEVPVS 350
    EKAKGLILRF CCEWEHRIGA PGVEEIKNNL FFEGVDWEHI RERPAAISIE 400
    IKSIDDTSNF DEFPESDILK PTVTTSSHPE TDYKNKDWVF INYTYKRFEG 450
    LTARGAIPSY MKAAK 465
    Length:465
    Mass (Da):54,174
    Last modified:December 1, 2001 - v1
    Checksum:i00E29F8963196750
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY292399 mRNA. Translation: AAP44997.1.
    BC009658 mRNA. Translation: AAH09658.1.
    CCDSiCCDS28589.1.
    RefSeqiNP_598876.1. NM_134115.2.
    XP_006536793.1. XM_006536730.1.
    UniGeneiMm.439986.

    Genome annotation databases

    EnsembliENSMUST00000009138; ENSMUSP00000009138; ENSMUSG00000024006.
    ENSMUST00000119274; ENSMUSP00000113657; ENSMUSG00000024006.
    GeneIDi106504.
    KEGGimmu:106504.
    UCSCiuc008bsa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY292399 mRNA. Translation: AAP44997.1 .
    BC009658 mRNA. Translation: AAH09658.1 .
    CCDSi CCDS28589.1.
    RefSeqi NP_598876.1. NM_134115.2.
    XP_006536793.1. XM_006536730.1.
    UniGenei Mm.439986.

    3D structure databases

    ProteinModelPortali Q91VJ4.
    SMRi Q91VJ4. Positions 23-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223068. 10 interactions.
    IntActi Q91VJ4. 11 interactions.

    PTM databases

    PhosphoSitei Q91VJ4.

    Proteomic databases

    MaxQBi Q91VJ4.
    PaxDbi Q91VJ4.
    PRIDEi Q91VJ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000009138 ; ENSMUSP00000009138 ; ENSMUSG00000024006 .
    ENSMUST00000119274 ; ENSMUSP00000113657 ; ENSMUSG00000024006 .
    GeneIDi 106504.
    KEGGi mmu:106504.
    UCSCi uc008bsa.1. mouse.

    Organism-specific databases

    CTDi 11329.
    MGIi MGI:2442572. Stk38.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117396.
    HOGENOMi HOG000233033.
    HOVERGENi HBG104247.
    InParanoidi Q91VJ4.
    KOi K08790.
    OMAi NAAYPPQ.
    OrthoDBi EOG7BZVS1.
    PhylomeDBi Q91VJ4.
    TreeFami TF105337.

    Miscellaneous databases

    ChiTaRSi STK38. mouse.
    NextBioi 358230.
    PROi Q91VJ4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91VJ4.
    Bgeei Q91VJ4.
    CleanExi MM_STK38.
    Genevestigatori Q91VJ4.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of NDR2 protein kinase by multi-site phosphorylation and the S100B calcium-binding protein."
      Stegert M.R., Tamaskovic R., Bichsel S.J., Hergovich A., Hemmings B.A.
      J. Biol. Chem. 279:23806-23812(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6Imported.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: Mammary glandImported.
    3. Cited for: FUNCTION, INTERACTION WITH MICAL1.

    Entry informationi

    Entry nameiSTK38_MOUSE
    AccessioniPrimary (citable) accession number: Q91VJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3