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Q91VJ1

- AIM2_MOUSE

UniProt

Q91VJ1 - AIM2_MOUSE

Protein

Interferon-inducible protein AIM2

Gene

Aim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (20 May 2008)
      Previous versions | rss
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    Functioni

    Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8.10 Publications

    Enzyme regulationi

    In absence of dsDNA DAPIN and HIN-20 domain can interact inducing a closed conformation; an autoinhibitory mechanism is proposed in which binding to dsDNA liberates the DAPIN domain for homotypic downstream signaling interactions with PYCARD.By similarity

    GO - Molecular functioni

    1. double-stranded DNA binding Source: UniProtKB
    2. identical protein binding Source: IntAct
    3. protein binding Source: MGI

    GO - Biological processi

    1. activation of innate immune response Source: UniProtKB
    2. apoptotic process Source: UniProtKB-KW
    3. cellular response to drug Source: MGI
    4. cellular response to interferon-beta Source: MGI
    5. inflammatory response Source: UniProtKB-KW
    6. innate immune response Source: UniProtKB-KW
    7. interleukin-1 beta secretion Source: MGI
    8. negative regulation of NF-kappaB transcription factor activity Source: Ensembl
    9. positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
    10. positive regulation of defense response to virus by host Source: UniProtKB
    11. positive regulation of interleukin-1 beta production Source: UniProtKB
    12. positive regulation of interleukin-1 beta secretion Source: MGI
    13. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
    14. positive regulation of protein oligomerization Source: Ensembl
    15. pyroptosis Source: UniProtKB
    16. tumor necrosis factor-mediated signaling pathway Source: Ensembl

    Keywords - Biological processi

    Apoptosis, Immunity, Inflammatory response, Innate immunity

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198662. The AIM2 inflammasome.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon-inducible protein AIM2
    Alternative name(s):
    Interferon-inducible protein 210
    Short name:
    Ifi-210
    Interferon-inducible protein p210
    Gene namesi
    Name:Aim2
    Synonyms:Gm1313, Ifi210
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2686159. Aim2.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. AIM2 inflammasome complex Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: Reactome
    4. mitochondrion Source: Ensembl
    5. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Interferon-inducible protein AIM2PRO_0000334528Add
    BLAST

    Proteomic databases

    PRIDEiQ91VJ1.

    PTM databases

    PhosphoSiteiQ91VJ1.

    Expressioni

    Gene expression databases

    BgeeiQ91VJ1.
    GenevestigatoriQ91VJ1.

    Interactioni

    Subunit structurei

    Self-associates; forms homooligomers in response to cytosolic dsDNA and the dsDNA seems to serve as oligomerization platform. Component of the AIM2 inflammasome complex. Interacts with PYCARD. Interacts with IFI16 By similarity. Interacts with EIF2AK2/PKR By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-6253384,EBI-6253384

    Protein-protein interaction databases

    IntActiQ91VJ1. 2 interactions.
    STRINGi10090.ENSMUSP00000119465.

    Structurei

    Secondary structure

    1
    354
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi160 – 1678
    Beta strandi181 – 1888
    Beta strandi193 – 1997
    Helixi202 – 2065
    Beta strandi212 – 2176
    Beta strandi225 – 2273
    Beta strandi231 – 2355
    Helixi244 – 2518
    Helixi256 – 2594
    Beta strandi267 – 27913
    Beta strandi281 – 29010
    Beta strandi293 – 3019
    Beta strandi313 – 32412
    Beta strandi327 – 3315
    Beta strandi337 – 3415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4JBMX-ray2.22A/B158-349[»]
    ProteinModelPortaliQ91VJ1.
    SMRiQ91VJ1. Positions 1-91, 158-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 8787DAPINPROSITE-ProRule annotationAdd
    BLAST
    Domaini144 – 341198HIN-200PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The DAPIN domain mediates homotypic interaction with PYCARD.By similarity
    The HIN-20 domain mediates dsDNA binding via electrostatic interactions.1 Publication

    Sequence similaritiesi

    Belongs to the HIN-200 family.Curated
    Contains 1 DAPIN domain.PROSITE-ProRule annotation
    Contains 1 HIN-200 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG132015.
    GeneTreeiENSGT00390000013296.
    HOVERGENiHBG006122.
    KOiK12966.
    OMAiFQKLNYM.
    OrthoDBiEOG78H3TT.
    PhylomeDBiQ91VJ1.
    TreeFamiTF337385.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    2.40.50.140. 2 hits.
    InterProiIPR004020. DAPIN.
    IPR011029. DEATH-like_dom.
    IPR004021. HIN200/IF120x.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF02760. HIN. 1 hit.
    PF02758. PYRIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    PROSITEiPS50824. DAPIN. 1 hit.
    PS50834. HIN_200. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91VJ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESEYREMLL LTGLDHITEE ELKRFKYFAL TEFQIARSTL DVADRTELAD    50
    HLIQSAGAAS AVTKAINIFQ KLNYMHIANA LEEKKKEAER KLMTNTKKRG 100
    TQKVENRSQA ENCSAASATR SDNDFKEQAA TEVCPQAKPQ KKQMVAEQEA 150
    IREDLQKDPL VVTVLKAINP FECETQEGRQ EIFHATVATE TDFFFVKVLN 200
    AQFKDKFIPK RTIKISNYLW HSNFMEVTSS SVVVDVESNH EVPNNVVKRA 250
    RETPRISKLK IQPCGTIVNG LFKVQKITEE KDRVLYGIHD KTGTMEVLVL 300
    GNPSKTKCEE GDKIRLTFFE VSKNGVKIQL KSGPCSFFKV IKAAKPKTDM 350
    KSVE 354
    Length:354
    Mass (Da):40,155
    Last modified:May 20, 2008 - v2
    Checksum:iE860C5D902AA5D35
    GO

    Sequence cautioni

    The sequence AAH09664.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC084073 Genomic DNA. No translation available.
    BC009664 mRNA. Translation: AAH09664.1. Different initiation.
    CCDSiCCDS15529.2.
    RefSeqiNP_001013801.2. NM_001013779.2.
    XP_006496978.1. XM_006496915.1.
    XP_006496979.1. XM_006496916.1.
    XP_006496980.1. XM_006496917.1.
    XP_006496981.1. XM_006496918.1.
    XP_006496982.1. XM_006496919.1.
    XP_006496983.1. XM_006496920.1.
    XP_006496984.1. XM_006496921.1.
    XP_006496985.1. XM_006496922.1.
    XP_006496986.1. XM_006496923.1.
    XP_006496987.1. XM_006496924.1.
    XP_006496988.1. XM_006496925.1.
    XP_006496989.1. XM_006496926.1.
    UniGeneiMm.131453.

    Genome annotation databases

    EnsembliENSMUST00000147604; ENSMUSP00000119465; ENSMUSG00000037860.
    ENSMUST00000166137; ENSMUSP00000132253; ENSMUSG00000037860.
    GeneIDi383619.
    KEGGimmu:383619.
    UCSCiuc011wws.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC084073 Genomic DNA. No translation available.
    BC009664 mRNA. Translation: AAH09664.1 . Different initiation.
    CCDSi CCDS15529.2.
    RefSeqi NP_001013801.2. NM_001013779.2.
    XP_006496978.1. XM_006496915.1.
    XP_006496979.1. XM_006496916.1.
    XP_006496980.1. XM_006496917.1.
    XP_006496981.1. XM_006496918.1.
    XP_006496982.1. XM_006496919.1.
    XP_006496983.1. XM_006496920.1.
    XP_006496984.1. XM_006496921.1.
    XP_006496985.1. XM_006496922.1.
    XP_006496986.1. XM_006496923.1.
    XP_006496987.1. XM_006496924.1.
    XP_006496988.1. XM_006496925.1.
    XP_006496989.1. XM_006496926.1.
    UniGenei Mm.131453.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4JBM X-ray 2.22 A/B 158-349 [» ]
    ProteinModelPortali Q91VJ1.
    SMRi Q91VJ1. Positions 1-91, 158-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91VJ1. 2 interactions.
    STRINGi 10090.ENSMUSP00000119465.

    PTM databases

    PhosphoSitei Q91VJ1.

    Proteomic databases

    PRIDEi Q91VJ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000147604 ; ENSMUSP00000119465 ; ENSMUSG00000037860 .
    ENSMUST00000166137 ; ENSMUSP00000132253 ; ENSMUSG00000037860 .
    GeneIDi 383619.
    KEGGi mmu:383619.
    UCSCi uc011wws.1. mouse.

    Organism-specific databases

    CTDi 9447.
    MGIi MGI:2686159. Aim2.

    Phylogenomic databases

    eggNOGi NOG132015.
    GeneTreei ENSGT00390000013296.
    HOVERGENi HBG006122.
    KOi K12966.
    OMAi FQKLNYM.
    OrthoDBi EOG78H3TT.
    PhylomeDBi Q91VJ1.
    TreeFami TF337385.

    Enzyme and pathway databases

    Reactomei REACT_198662. The AIM2 inflammasome.

    Miscellaneous databases

    NextBioi 404044.
    PROi Q91VJ1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91VJ1.
    Genevestigatori Q91VJ1.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    2.40.50.140. 2 hits.
    InterProi IPR004020. DAPIN.
    IPR011029. DEATH-like_dom.
    IPR004021. HIN200/IF120x.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF02760. HIN. 1 hit.
    PF02758. PYRIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    PROSITEi PS50824. DAPIN. 1 hit.
    PS50834. HIN_200. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 219-354.
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "The HIN-200 family: more than interferon-inducible genes?"
      Ludlow L.E.A., Johnstone R.W., Clarke C.J.P.
      Exp. Cell Res. 308:1-17(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA."
      Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S.
      Nature 458:509-513(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC."
      Hornung V., Ablasser A., Charrel-Dennis M., Bauernfeind F., Horvath G., Caffrey D.R., Latz E., Fitzgerald K.A.
      Nature 458:514-518(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNA."
      Roberts T.L., Idris A., Dunn J.A., Kelly G.M., Burnton C.M., Hodgson S., Hardy L.L., Garceau V., Sweet M.J., Ross I.L., Hume D.A., Stacey K.J.
      Science 323:1057-1060(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Listeria monocytogenes triggers AIM2-mediated pyroptosis upon infrequent bacteriolysis in the macrophage cytosol."
      Sauer J.D., Witte C.E., Zemansky J., Hanson B., Lauer P., Portnoy D.A.
      Cell Host Microbe 7:412-419(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: FUNCTION.
    9. Cited for: FUNCTION.
    10. "Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis."
      Jones J.W., Kayagaki N., Broz P., Henry T., Newton K., O'Rourke K., Chan S., Dong J., Qu Y., Roose-Girma M., Dixit V.M., Monack D.M.
      Proc. Natl. Acad. Sci. U.S.A. 107:9771-9776(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages."
      Pierini R., Juruj C., Perret M., Jones C.L., Mangeot P., Weiss D.S., Henry T.
      Cell Death Differ. 19:1709-1721(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Francisella infection triggers activation of the AIM2 inflammasome in murine dendritic cells."
      Belhocine K., Monack D.M.
      Cell. Microbiol. 14:71-80(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Structural basis for termination of AIM2-mediated signaling by p202."
      Ru H., Ni X., Zhao L., Crowley C., Ding W., Hung L.W., Shaw N., Cheng G., Liu Z.J.
      Cell Res. 23:855-858(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF 158-349 IN COMPLEX WITH DNA, FUNCTION, DOMAIN, DNA-BINDING.

    Entry informationi

    Entry nameiAIM2_MOUSE
    AccessioniPrimary (citable) accession number: Q91VJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3