Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q91VJ1

- AIM2_MOUSE

UniProt

Q91VJ1 - AIM2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Interferon-inducible protein AIM2

Gene

Aim2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8.10 Publications

Enzyme regulationi

In absence of dsDNA DAPIN and HIN-20 domain can interact inducing a closed conformation; an autoinhibitory mechanism is proposed in which binding to dsDNA liberates the DAPIN domain for homotypic downstream signaling interactions with PYCARD.By similarity

GO - Molecular functioni

  1. double-stranded DNA binding Source: UniProtKB
  2. identical protein binding Source: IntAct

GO - Biological processi

  1. activation of innate immune response Source: UniProtKB
  2. apoptotic process Source: UniProtKB-KW
  3. cellular response to drug Source: MGI
  4. cellular response to interferon-beta Source: MGI
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. interleukin-1 beta secretion Source: MGI
  8. negative regulation of NF-kappaB transcription factor activity Source: Ensembl
  9. positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
  10. positive regulation of defense response to virus by host Source: UniProtKB
  11. positive regulation of interleukin-1 beta production Source: UniProtKB
  12. positive regulation of interleukin-1 beta secretion Source: MGI
  13. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
  14. positive regulation of protein oligomerization Source: Ensembl
  15. pyroptosis Source: UniProtKB
  16. tumor necrosis factor-mediated signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Immunity, Inflammatory response, Innate immunity

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198662. The AIM2 inflammasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-inducible protein AIM2
Alternative name(s):
Interferon-inducible protein 210
Short name:
Ifi-210
Interferon-inducible protein p210
Gene namesi
Name:Aim2
Synonyms:Gm1313, Ifi210
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2686159. Aim2.

Subcellular locationi

Nucleus By similarity. Cytoplasm 1 Publication

GO - Cellular componenti

  1. AIM2 inflammasome complex Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Interferon-inducible protein AIM2PRO_0000334528Add
BLAST

Proteomic databases

MaxQBiQ91VJ1.
PRIDEiQ91VJ1.

PTM databases

PhosphoSiteiQ91VJ1.

Expressioni

Gene expression databases

BgeeiQ91VJ1.
ExpressionAtlasiQ91VJ1. baseline and differential.
GenevestigatoriQ91VJ1.

Interactioni

Subunit structurei

Self-associates; forms homooligomers in response to cytosolic dsDNA and the dsDNA seems to serve as oligomerization platform. Component of the AIM2 inflammasome complex. Interacts with PYCARD. Interacts with IFI16 (By similarity). Interacts with EIF2AK2/PKR (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6253384,EBI-6253384

Protein-protein interaction databases

IntActiQ91VJ1. 2 interactions.
STRINGi10090.ENSMUSP00000119465.

Structurei

Secondary structure

1
354
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi160 – 1678
Beta strandi181 – 1888
Beta strandi193 – 1997
Helixi202 – 2065
Beta strandi212 – 2176
Beta strandi225 – 2273
Beta strandi231 – 2355
Helixi244 – 2518
Helixi256 – 2594
Beta strandi267 – 27913
Beta strandi281 – 29010
Beta strandi293 – 3019
Beta strandi313 – 32412
Beta strandi327 – 3315
Beta strandi337 – 3415

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JBMX-ray2.22A/B158-349[»]
ProteinModelPortaliQ91VJ1.
SMRiQ91VJ1. Positions 1-91, 158-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8787DAPINPROSITE-ProRule annotationAdd
BLAST
Domaini144 – 341198HIN-200PROSITE-ProRule annotationAdd
BLAST

Domaini

The DAPIN domain mediates homotypic interaction with PYCARD.By similarity
The HIN-20 domain mediates dsDNA binding via electrostatic interactions.1 Publication

Sequence similaritiesi

Belongs to the HIN-200 family.Curated
Contains 1 DAPIN domain.PROSITE-ProRule annotation
Contains 1 HIN-200 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG132015.
GeneTreeiENSGT00390000013296.
HOVERGENiHBG006122.
InParanoidiQ91VJ1.
KOiK12966.
OMAiFQKLNYM.
OrthoDBiEOG78H3TT.
PhylomeDBiQ91VJ1.
TreeFamiTF337385.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.40.50.140. 2 hits.
InterProiIPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR004021. HIN200/IF120x.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF02760. HIN. 1 hit.
PF02758. PYRIN. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50824. DAPIN. 1 hit.
PS50834. HIN_200. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VJ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESEYREMLL LTGLDHITEE ELKRFKYFAL TEFQIARSTL DVADRTELAD
60 70 80 90 100
HLIQSAGAAS AVTKAINIFQ KLNYMHIANA LEEKKKEAER KLMTNTKKRG
110 120 130 140 150
TQKVENRSQA ENCSAASATR SDNDFKEQAA TEVCPQAKPQ KKQMVAEQEA
160 170 180 190 200
IREDLQKDPL VVTVLKAINP FECETQEGRQ EIFHATVATE TDFFFVKVLN
210 220 230 240 250
AQFKDKFIPK RTIKISNYLW HSNFMEVTSS SVVVDVESNH EVPNNVVKRA
260 270 280 290 300
RETPRISKLK IQPCGTIVNG LFKVQKITEE KDRVLYGIHD KTGTMEVLVL
310 320 330 340 350
GNPSKTKCEE GDKIRLTFFE VSKNGVKIQL KSGPCSFFKV IKAAKPKTDM

KSVE
Length:354
Mass (Da):40,155
Last modified:May 20, 2008 - v2
Checksum:iE860C5D902AA5D35
GO

Sequence cautioni

The sequence AAH09664.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC084073 Genomic DNA. No translation available.
BC009664 mRNA. Translation: AAH09664.1. Different initiation.
CCDSiCCDS15529.2.
RefSeqiNP_001013801.2. NM_001013779.2.
XP_006496978.1. XM_006496915.1.
XP_006496979.1. XM_006496916.1.
XP_006496980.1. XM_006496917.1.
XP_006496981.1. XM_006496918.1.
XP_006496982.1. XM_006496919.1.
XP_006496983.1. XM_006496920.1.
XP_006496984.1. XM_006496921.1.
XP_006496985.1. XM_006496922.1.
XP_006496986.1. XM_006496923.1.
XP_006496987.1. XM_006496924.1.
XP_006496988.1. XM_006496925.1.
XP_006496989.1. XM_006496926.1.
UniGeneiMm.131453.

Genome annotation databases

EnsembliENSMUST00000147604; ENSMUSP00000119465; ENSMUSG00000037860.
ENSMUST00000166137; ENSMUSP00000132253; ENSMUSG00000037860.
GeneIDi383619.
KEGGimmu:383619.
UCSCiuc011wws.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC084073 Genomic DNA. No translation available.
BC009664 mRNA. Translation: AAH09664.1 . Different initiation.
CCDSi CCDS15529.2.
RefSeqi NP_001013801.2. NM_001013779.2.
XP_006496978.1. XM_006496915.1.
XP_006496979.1. XM_006496916.1.
XP_006496980.1. XM_006496917.1.
XP_006496981.1. XM_006496918.1.
XP_006496982.1. XM_006496919.1.
XP_006496983.1. XM_006496920.1.
XP_006496984.1. XM_006496921.1.
XP_006496985.1. XM_006496922.1.
XP_006496986.1. XM_006496923.1.
XP_006496987.1. XM_006496924.1.
XP_006496988.1. XM_006496925.1.
XP_006496989.1. XM_006496926.1.
UniGenei Mm.131453.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4JBM X-ray 2.22 A/B 158-349 [» ]
ProteinModelPortali Q91VJ1.
SMRi Q91VJ1. Positions 1-91, 158-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91VJ1. 2 interactions.
STRINGi 10090.ENSMUSP00000119465.

PTM databases

PhosphoSitei Q91VJ1.

Proteomic databases

MaxQBi Q91VJ1.
PRIDEi Q91VJ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000147604 ; ENSMUSP00000119465 ; ENSMUSG00000037860 .
ENSMUST00000166137 ; ENSMUSP00000132253 ; ENSMUSG00000037860 .
GeneIDi 383619.
KEGGi mmu:383619.
UCSCi uc011wws.1. mouse.

Organism-specific databases

CTDi 9447.
MGIi MGI:2686159. Aim2.

Phylogenomic databases

eggNOGi NOG132015.
GeneTreei ENSGT00390000013296.
HOVERGENi HBG006122.
InParanoidi Q91VJ1.
KOi K12966.
OMAi FQKLNYM.
OrthoDBi EOG78H3TT.
PhylomeDBi Q91VJ1.
TreeFami TF337385.

Enzyme and pathway databases

Reactomei REACT_198662. The AIM2 inflammasome.

Miscellaneous databases

NextBioi 404044.
PROi Q91VJ1.
SOURCEi Search...

Gene expression databases

Bgeei Q91VJ1.
ExpressionAtlasi Q91VJ1. baseline and differential.
Genevestigatori Q91VJ1.

Family and domain databases

Gene3Di 1.10.533.10. 1 hit.
2.40.50.140. 2 hits.
InterProi IPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR004021. HIN200/IF120x.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF02760. HIN. 1 hit.
PF02758. PYRIN. 1 hit.
[Graphical view ]
SUPFAMi SSF47986. SSF47986. 1 hit.
PROSITEi PS50824. DAPIN. 1 hit.
PS50834. HIN_200. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 219-354.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "The HIN-200 family: more than interferon-inducible genes?"
    Ludlow L.E.A., Johnstone R.W., Clarke C.J.P.
    Exp. Cell Res. 308:1-17(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA."
    Fernandes-Alnemri T., Yu J.W., Datta P., Wu J., Alnemri E.S.
    Nature 458:509-513(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC."
    Hornung V., Ablasser A., Charrel-Dennis M., Bauernfeind F., Horvath G., Caffrey D.R., Latz E., Fitzgerald K.A.
    Nature 458:514-518(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNA."
    Roberts T.L., Idris A., Dunn J.A., Kelly G.M., Burnton C.M., Hodgson S., Hardy L.L., Garceau V., Sweet M.J., Ross I.L., Hume D.A., Stacey K.J.
    Science 323:1057-1060(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Listeria monocytogenes triggers AIM2-mediated pyroptosis upon infrequent bacteriolysis in the macrophage cytosol."
    Sauer J.D., Witte C.E., Zemansky J., Hanson B., Lauer P., Portnoy D.A.
    Cell Host Microbe 7:412-419(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: FUNCTION.
  9. Cited for: FUNCTION.
  10. "Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis."
    Jones J.W., Kayagaki N., Broz P., Henry T., Newton K., O'Rourke K., Chan S., Dong J., Qu Y., Roose-Girma M., Dixit V.M., Monack D.M.
    Proc. Natl. Acad. Sci. U.S.A. 107:9771-9776(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages."
    Pierini R., Juruj C., Perret M., Jones C.L., Mangeot P., Weiss D.S., Henry T.
    Cell Death Differ. 19:1709-1721(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "Francisella infection triggers activation of the AIM2 inflammasome in murine dendritic cells."
    Belhocine K., Monack D.M.
    Cell. Microbiol. 14:71-80(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Structural basis for termination of AIM2-mediated signaling by p202."
    Ru H., Ni X., Zhao L., Crowley C., Ding W., Hung L.W., Shaw N., Cheng G., Liu Z.J.
    Cell Res. 23:855-858(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF 158-349 IN COMPLEX WITH DNA, FUNCTION, DOMAIN, DNA-BINDING.

Entry informationi

Entry nameiAIM2_MOUSE
AccessioniPrimary (citable) accession number: Q91VJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3