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Protein

Sorting nexin-9

Gene

Snx9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei286 – 2861Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei288 – 2881Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei313 – 3131Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei327 – 3271Phosphatidylinositol 4,5-bisphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Endocytosis, Mitosis, Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiREACT_342454. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-9
Gene namesi
Name:Snx9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1913866. Snx9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Sorting nexin-9PRO_0000213853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei239 – 2391Phosphotyrosine1 Publication
Modified residuei288 – 2881N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR (By similarity).By similarity
Ubiquitinated by ITCH.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ91VH2.
PaxDbiQ91VH2.
PRIDEiQ91VH2.

PTM databases

PhosphoSiteiQ91VH2.

Expressioni

Gene expression databases

BgeeiQ91VH2.
CleanExiMM_SNX9.
ExpressionAtlasiQ91VH2. baseline and differential.
GenevisibleiQ91VH2. MM.

Interactioni

Subunit structurei

Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ARP3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails.By similarity1 Publication

Protein-protein interaction databases

BioGridi211598. 2 interactions.
DIPiDIP-60857N.
IntActiQ91VH2. 1 interaction.
MINTiMINT-1866620.
STRINGi10090.ENSMUSP00000002436.

Structurei

Secondary structure

1
595
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Beta strandi17 – 193Combined sources
Beta strandi27 – 337Combined sources
Beta strandi36 – 438Combined sources
Beta strandi49 – 535Combined sources
Turni54 – 563Combined sources
Beta strandi57 – 604Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ENMNMR-A1-70[»]
ProteinModelPortaliQ91VH2.
SMRiQ91VH2. Positions 1-70, 214-595.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91VH2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6262SH3PROSITE-ProRule annotationAdd
BLAST
Domaini250 – 360111PXPROSITE-ProRule annotationAdd
BLAST
Domaini392 – 595204BARAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 21313Critical for tubulation activityBy similarityAdd
BLAST

Domaini

The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 BAR domain.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG5391.
GeneTreeiENSGT00510000046469.
HOGENOMiHOG000261633.
HOVERGENiHBG009996.
InParanoidiQ91VH2.
KOiK17923.
OMAiDPWSAWN.
OrthoDBiEOG7GTT36.
PhylomeDBiQ91VH2.
TreeFamiTF314082.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
IPR028644. SNX9.
IPR014536. Snx9_subfam.
IPR019497. Sorting_nexin_WASP-bd-dom.
[Graphical view]
PANTHERiPTHR10555:SF14. PTHR10555:SF14. 1 hit.
PfamiPF10456. BAR_3_WASP_bdg. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF027744. Snx9. 1 hit.
SMARTiSM00312. PX. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKARVMYD FAAEPGNNEL TVTEGEIITV TNPNVGGGWL EGKNNKGEQG
60 70 80 90 100
LVPTDYVEIL PNDGKDPFSC GNSVADQAFL DSLTASTAQT NSSSANSNNQ
110 120 130 140 150
VGGGNDPWTA WNAPKPGNWD SSDAWGSRTD GTSAQRNSSA NNWDTGFGHP
160 170 180 190 200
QAYQGPATGD DDEWDEDWDD PKSSSPYFKD SEPAEAGGIQ RGNSRAGASS
210 220 230 240 250
MKLPLNKFPG FAKPGMEQYL LAKQLAKPKE KIAIIVGDYG PMWVYPTSTF
260 270 280 290 300
DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK
310 320 330 340 350
FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVVSESEVF
360 370 380 390 400
QQFLNFRDEK EWKTGKRKAE KDELVGVMIF STMEPEAPDL DLIEIEQKCD
410 420 430 440 450
AVGKFTKAMD DGVKELLTVG QEHWKRCTGP LPKEYQKIGK ALQSLAAVFS
460 470 480 490 500
SSGYQGETDL NDAITEAGKT YEEIASLVAE QPKKDLHFLM ECNHEYKGFL
510 520 530 540 550
GCFPDIIGAH KGAIEKVKES DKLVATSKIT PQDKQTMVKR VGTMSYALQA
560 570 580 590
EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM
Length:595
Mass (Da):66,546
Last modified:December 1, 2001 - v1
Checksum:i3D5568476F2D816D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014814 mRNA. Translation: AAH14814.1.
CCDSiCCDS57041.1.
RefSeqiNP_079940.2. NM_025664.5.
UniGeneiMm.89515.

Genome annotation databases

EnsembliENSMUST00000002436; ENSMUSP00000002436; ENSMUSG00000002365.
GeneIDi66616.
KEGGimmu:66616.
UCSCiuc008afj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014814 mRNA. Translation: AAH14814.1.
CCDSiCCDS57041.1.
RefSeqiNP_079940.2. NM_025664.5.
UniGeneiMm.89515.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ENMNMR-A1-70[»]
ProteinModelPortaliQ91VH2.
SMRiQ91VH2. Positions 1-70, 214-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211598. 2 interactions.
DIPiDIP-60857N.
IntActiQ91VH2. 1 interaction.
MINTiMINT-1866620.
STRINGi10090.ENSMUSP00000002436.

PTM databases

PhosphoSiteiQ91VH2.

Proteomic databases

MaxQBiQ91VH2.
PaxDbiQ91VH2.
PRIDEiQ91VH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002436; ENSMUSP00000002436; ENSMUSG00000002365.
GeneIDi66616.
KEGGimmu:66616.
UCSCiuc008afj.1. mouse.

Organism-specific databases

CTDi51429.
MGIiMGI:1913866. Snx9.

Phylogenomic databases

eggNOGiCOG5391.
GeneTreeiENSGT00510000046469.
HOGENOMiHOG000261633.
HOVERGENiHBG009996.
InParanoidiQ91VH2.
KOiK17923.
OMAiDPWSAWN.
OrthoDBiEOG7GTT36.
PhylomeDBiQ91VH2.
TreeFamiTF314082.

Enzyme and pathway databases

ReactomeiREACT_342454. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiSnx9. mouse.
EvolutionaryTraceiQ91VH2.
NextBioi322180.
PROiQ91VH2.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VH2.
CleanExiMM_SNX9.
ExpressionAtlasiQ91VH2. baseline and differential.
GenevisibleiQ91VH2. MM.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
IPR028644. SNX9.
IPR014536. Snx9_subfam.
IPR019497. Sorting_nexin_WASP-bd-dom.
[Graphical view]
PANTHERiPTHR10555:SF14. PTHR10555:SF14. 1 hit.
PfamiPF10456. BAR_3_WASP_bdg. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF027744. Snx9. 1 hit.
SMARTiSM00312. PX. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  2. "Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
    Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
    J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ADAM9 AND ADAM15.
  3. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Solution structure of the SH3 domain from mouse sorting nexin-9."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-70.

Entry informationi

Entry nameiSNX9_MOUSE
AccessioniPrimary (citable) accession number: Q91VH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.