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Q91VH2

- SNX9_MOUSE

UniProt

Q91VH2 - SNX9_MOUSE

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Protein

Sorting nexin-9

Gene

Snx9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei286 – 2861Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei288 – 2881Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei313 – 3131Phosphatidylinositol 4,5-bisphosphateBy similarity
Binding sitei327 – 3271Phosphatidylinositol 4,5-bisphosphateBy similarity

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: UniProtKB
  2. phosphatidylinositol binding Source: MGI

GO - Biological processi

  1. cleavage furrow formation Source: UniProtKB
  2. endocytosis Source: UniProtKB
  3. endosomal transport Source: UniProtKB
  4. intracellular protein transport Source: MGI
  5. lipid tube assembly Source: UniProtKB
  6. mitotic cytokinesis Source: UniProtKB
  7. mitotic nuclear division Source: UniProtKB-KW
  8. positive regulation of GTPase activity Source: UniProtKB
  9. positive regulation of protein oligomerization Source: UniProtKB
  10. receptor-mediated endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Endocytosis, Mitosis, Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-9
Gene namesi
Name:Snx9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1913866. Snx9.

Subcellular locationi

Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Golgi apparatustrans-Golgi network By similarity. Cell projectionruffle By similarity. Cytoplasm By similarity
Note: Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation (By similarity). Colocalizes with the actin cytoskeleton at the cell membrane (By similarity).By similarity

GO - Cellular componenti

  1. cell projection Source: UniProtKB-KW
  2. clathrin-coated vesicle Source: Ensembl
  3. cytoplasm Source: MGI
  4. cytoplasmic membrane-bounded vesicle Source: UniProtKB
  5. cytoplasmic vesicle membrane Source: UniProtKB
  6. endosome Source: RefGenome
  7. extracellular vesicular exosome Source: Ensembl
  8. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  9. plasma membrane Source: MGI
  10. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Sorting nexin-9PRO_0000213853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei239 – 2391Phosphotyrosine1 Publication
Modified residuei288 – 2881N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR (By similarity).By similarity
Ubiquitinated by ITCH.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ91VH2.
PaxDbiQ91VH2.
PRIDEiQ91VH2.

PTM databases

PhosphoSiteiQ91VH2.

Expressioni

Gene expression databases

BgeeiQ91VH2.
CleanExiMM_SNX9.
ExpressionAtlasiQ91VH2. baseline and differential.
GenevestigatoriQ91VH2.

Interactioni

Subunit structurei

Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ARP3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails.By similarity1 Publication

Protein-protein interaction databases

BioGridi211598. 2 interactions.
DIPiDIP-60857N.
IntActiQ91VH2. 1 interaction.
MINTiMINT-1866620.

Structurei

Secondary structure

1
595
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97
Beta strandi17 – 193
Beta strandi27 – 337
Beta strandi36 – 438
Beta strandi49 – 535
Turni54 – 563
Beta strandi57 – 604

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ENMNMR-A1-70[»]
ProteinModelPortaliQ91VH2.
SMRiQ91VH2. Positions 1-70, 214-595.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91VH2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6262SH3PROSITE-ProRule annotationAdd
BLAST
Domaini250 – 360111PXPROSITE-ProRule annotationAdd
BLAST
Domaini392 – 595204BARAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 21313Critical for tubulation activityBy similarityAdd
BLAST

Domaini

The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 BAR domain.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiCOG5391.
GeneTreeiENSGT00510000046469.
HOGENOMiHOG000261633.
HOVERGENiHBG009996.
InParanoidiQ91VH2.
KOiK17923.
OMAiDPWSAWN.
OrthoDBiEOG7GTT36.
PhylomeDBiQ91VH2.
TreeFamiTF314082.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
IPR028644. SNX9.
IPR014536. Snx9_subfam.
IPR019497. Sorting_nexin_WASP-bd-dom.
[Graphical view]
PANTHERiPTHR10555:SF14. PTHR10555:SF14. 1 hit.
PfamiPF10456. BAR_3_WASP_bdg. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF027744. Snx9. 1 hit.
SMARTiSM00312. PX. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VH2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATKARVMYD FAAEPGNNEL TVTEGEIITV TNPNVGGGWL EGKNNKGEQG
60 70 80 90 100
LVPTDYVEIL PNDGKDPFSC GNSVADQAFL DSLTASTAQT NSSSANSNNQ
110 120 130 140 150
VGGGNDPWTA WNAPKPGNWD SSDAWGSRTD GTSAQRNSSA NNWDTGFGHP
160 170 180 190 200
QAYQGPATGD DDEWDEDWDD PKSSSPYFKD SEPAEAGGIQ RGNSRAGASS
210 220 230 240 250
MKLPLNKFPG FAKPGMEQYL LAKQLAKPKE KIAIIVGDYG PMWVYPTSTF
260 270 280 290 300
DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK
310 320 330 340 350
FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVVSESEVF
360 370 380 390 400
QQFLNFRDEK EWKTGKRKAE KDELVGVMIF STMEPEAPDL DLIEIEQKCD
410 420 430 440 450
AVGKFTKAMD DGVKELLTVG QEHWKRCTGP LPKEYQKIGK ALQSLAAVFS
460 470 480 490 500
SSGYQGETDL NDAITEAGKT YEEIASLVAE QPKKDLHFLM ECNHEYKGFL
510 520 530 540 550
GCFPDIIGAH KGAIEKVKES DKLVATSKIT PQDKQTMVKR VGTMSYALQA
560 570 580 590
EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM
Length:595
Mass (Da):66,546
Last modified:December 1, 2001 - v1
Checksum:i3D5568476F2D816D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC014814 mRNA. Translation: AAH14814.1.
CCDSiCCDS57041.1.
RefSeqiNP_079940.2. NM_025664.5.
UniGeneiMm.89515.

Genome annotation databases

EnsembliENSMUST00000002436; ENSMUSP00000002436; ENSMUSG00000002365.
GeneIDi66616.
KEGGimmu:66616.
UCSCiuc008afj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC014814 mRNA. Translation: AAH14814.1 .
CCDSi CCDS57041.1.
RefSeqi NP_079940.2. NM_025664.5.
UniGenei Mm.89515.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ENM NMR - A 1-70 [» ]
ProteinModelPortali Q91VH2.
SMRi Q91VH2. Positions 1-70, 214-595.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211598. 2 interactions.
DIPi DIP-60857N.
IntActi Q91VH2. 1 interaction.
MINTi MINT-1866620.

PTM databases

PhosphoSitei Q91VH2.

Proteomic databases

MaxQBi Q91VH2.
PaxDbi Q91VH2.
PRIDEi Q91VH2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002436 ; ENSMUSP00000002436 ; ENSMUSG00000002365 .
GeneIDi 66616.
KEGGi mmu:66616.
UCSCi uc008afj.1. mouse.

Organism-specific databases

CTDi 51429.
MGIi MGI:1913866. Snx9.

Phylogenomic databases

eggNOGi COG5391.
GeneTreei ENSGT00510000046469.
HOGENOMi HOG000261633.
HOVERGENi HBG009996.
InParanoidi Q91VH2.
KOi K17923.
OMAi DPWSAWN.
OrthoDBi EOG7GTT36.
PhylomeDBi Q91VH2.
TreeFami TF314082.

Miscellaneous databases

ChiTaRSi SNX9. mouse.
EvolutionaryTracei Q91VH2.
NextBioi 322180.
PROi Q91VH2.
SOURCEi Search...

Gene expression databases

Bgeei Q91VH2.
CleanExi MM_SNX9.
ExpressionAtlasi Q91VH2. baseline and differential.
Genevestigatori Q91VH2.

Family and domain databases

Gene3Di 3.30.1520.10. 1 hit.
InterProi IPR001683. Phox.
IPR001452. SH3_domain.
IPR028644. SNX9.
IPR014536. Snx9_subfam.
IPR019497. Sorting_nexin_WASP-bd-dom.
[Graphical view ]
PANTHERi PTHR10555:SF14. PTHR10555:SF14. 1 hit.
Pfami PF10456. BAR_3_WASP_bdg. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF027744. Snx9. 1 hit.
SMARTi SM00312. PX. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEi PS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  2. "Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
    Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
    J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ADAM9 AND ADAM15.
  3. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Solution structure of the SH3 domain from mouse sorting nexin-9."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-70.

Entry informationi

Entry nameiSNX9_MOUSE
AccessioniPrimary (citable) accession number: Q91VH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3