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Q91VH2

- SNX9_MOUSE

UniProt

Q91VH2 - SNX9_MOUSE

Protein

Sorting nexin-9

Gene

Snx9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei286 – 2861Phosphatidylinositol 4,5-bisphosphateBy similarity
    Binding sitei288 – 2881Phosphatidylinositol 4,5-bisphosphateBy similarity
    Binding sitei313 – 3131Phosphatidylinositol 4,5-bisphosphateBy similarity
    Binding sitei327 – 3271Phosphatidylinositol 4,5-bisphosphateBy similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: UniProtKB
    2. phosphatidylinositol binding Source: MGI
    3. protein binding Source: MGI

    GO - Biological processi

    1. cleavage furrow formation Source: UniProtKB
    2. endocytosis Source: UniProtKB
    3. endosomal transport Source: UniProtKB
    4. intracellular protein transport Source: MGI
    5. lipid tube assembly Source: UniProtKB
    6. mitotic cytokinesis Source: UniProtKB
    7. mitotic nuclear division Source: UniProtKB-KW
    8. positive regulation of GTPase activity Source: UniProtKB
    9. positive regulation of protein oligomerization Source: UniProtKB
    10. receptor-mediated endocytosis Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, Endocytosis, Mitosis, Protein transport, Transport

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sorting nexin-9
    Gene namesi
    Name:Snx9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1913866. Snx9.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Golgi apparatustrans-Golgi network By similarity. Cell projectionruffle By similarity. Cytoplasm By similarity
    Note: Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation By similarity. Colocalizes with the actin cytoskeleton at the cell membrane By similarity.By similarity

    GO - Cellular componenti

    1. clathrin-coated vesicle Source: UniProtKB-SubCell
    2. cytoplasm Source: MGI
    3. cytoplasmic membrane-bounded vesicle Source: UniProtKB
    4. cytoplasmic vesicle membrane Source: UniProtKB
    5. endosome Source: RefGenome
    6. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    7. plasma membrane Source: MGI
    8. ruffle Source: UniProtKB-SubCell
    9. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595Sorting nexin-9PRO_0000213853Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei239 – 2391Phosphotyrosine1 Publication
    Modified residuei288 – 2881N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR By similarity.By similarity
    Ubiquitinated by ITCH.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ91VH2.
    PaxDbiQ91VH2.
    PRIDEiQ91VH2.

    PTM databases

    PhosphoSiteiQ91VH2.

    Expressioni

    Gene expression databases

    BgeeiQ91VH2.
    CleanExiMM_SNX9.
    GenevestigatoriQ91VH2.

    Interactioni

    Subunit structurei

    Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ARP3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9 By similarity. Interacts with ADAM9 and ADAM15 cytoplasmic tails.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi211598. 2 interactions.
    DIPiDIP-60857N.
    IntActiQ91VH2. 1 interaction.
    MINTiMINT-1866620.

    Structurei

    Secondary structure

    1
    595
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Beta strandi17 – 193
    Beta strandi27 – 337
    Beta strandi36 – 438
    Beta strandi49 – 535
    Turni54 – 563
    Beta strandi57 – 604

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ENMNMR-A1-70[»]
    ProteinModelPortaliQ91VH2.
    SMRiQ91VH2. Positions 1-70, 214-595.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ91VH2.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6262SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini250 – 360111PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini392 – 595204BARAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni201 – 21313Critical for tubulation activityBy similarityAdd
    BLAST

    Domaini

    The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates By similarity.By similarity

    Sequence similaritiesi

    Belongs to the sorting nexin family.Curated
    Contains 1 BAR domain.Curated
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG5391.
    GeneTreeiENSGT00510000046469.
    HOGENOMiHOG000261633.
    HOVERGENiHBG009996.
    InParanoidiQ91VH2.
    KOiK17923.
    OMAiDPWSAWN.
    OrthoDBiEOG7GTT36.
    PhylomeDBiQ91VH2.
    TreeFamiTF314082.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    IPR001452. SH3_domain.
    IPR028644. SNX9.
    IPR014536. Snx9_subfam.
    IPR019497. Sorting_nexin_WASP-bd-dom.
    [Graphical view]
    PANTHERiPTHR10555:SF14. PTHR10555:SF14. 1 hit.
    PfamiPF10456. BAR_3_WASP_bdg. 1 hit.
    PF00787. PX. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF027744. Snx9. 1 hit.
    SMARTiSM00312. PX. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91VH2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATKARVMYD FAAEPGNNEL TVTEGEIITV TNPNVGGGWL EGKNNKGEQG    50
    LVPTDYVEIL PNDGKDPFSC GNSVADQAFL DSLTASTAQT NSSSANSNNQ 100
    VGGGNDPWTA WNAPKPGNWD SSDAWGSRTD GTSAQRNSSA NNWDTGFGHP 150
    QAYQGPATGD DDEWDEDWDD PKSSSPYFKD SEPAEAGGIQ RGNSRAGASS 200
    MKLPLNKFPG FAKPGMEQYL LAKQLAKPKE KIAIIVGDYG PMWVYPTSTF 250
    DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK 300
    FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVVSESEVF 350
    QQFLNFRDEK EWKTGKRKAE KDELVGVMIF STMEPEAPDL DLIEIEQKCD 400
    AVGKFTKAMD DGVKELLTVG QEHWKRCTGP LPKEYQKIGK ALQSLAAVFS 450
    SSGYQGETDL NDAITEAGKT YEEIASLVAE QPKKDLHFLM ECNHEYKGFL 500
    GCFPDIIGAH KGAIEKVKES DKLVATSKIT PQDKQTMVKR VGTMSYALQA 550
    EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM 595
    Length:595
    Mass (Da):66,546
    Last modified:December 1, 2001 - v1
    Checksum:i3D5568476F2D816D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC014814 mRNA. Translation: AAH14814.1.
    CCDSiCCDS57041.1.
    RefSeqiNP_079940.2. NM_025664.5.
    UniGeneiMm.89515.

    Genome annotation databases

    EnsembliENSMUST00000002436; ENSMUSP00000002436; ENSMUSG00000002365.
    GeneIDi66616.
    KEGGimmu:66616.
    UCSCiuc008afj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC014814 mRNA. Translation: AAH14814.1 .
    CCDSi CCDS57041.1.
    RefSeqi NP_079940.2. NM_025664.5.
    UniGenei Mm.89515.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ENM NMR - A 1-70 [» ]
    ProteinModelPortali Q91VH2.
    SMRi Q91VH2. Positions 1-70, 214-595.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211598. 2 interactions.
    DIPi DIP-60857N.
    IntActi Q91VH2. 1 interaction.
    MINTi MINT-1866620.

    PTM databases

    PhosphoSitei Q91VH2.

    Proteomic databases

    MaxQBi Q91VH2.
    PaxDbi Q91VH2.
    PRIDEi Q91VH2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002436 ; ENSMUSP00000002436 ; ENSMUSG00000002365 .
    GeneIDi 66616.
    KEGGi mmu:66616.
    UCSCi uc008afj.1. mouse.

    Organism-specific databases

    CTDi 51429.
    MGIi MGI:1913866. Snx9.

    Phylogenomic databases

    eggNOGi COG5391.
    GeneTreei ENSGT00510000046469.
    HOGENOMi HOG000261633.
    HOVERGENi HBG009996.
    InParanoidi Q91VH2.
    KOi K17923.
    OMAi DPWSAWN.
    OrthoDBi EOG7GTT36.
    PhylomeDBi Q91VH2.
    TreeFami TF314082.

    Miscellaneous databases

    ChiTaRSi SNX9. mouse.
    EvolutionaryTracei Q91VH2.
    NextBioi 322180.
    PROi Q91VH2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91VH2.
    CleanExi MM_SNX9.
    Genevestigatori Q91VH2.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    IPR001452. SH3_domain.
    IPR028644. SNX9.
    IPR014536. Snx9_subfam.
    IPR019497. Sorting_nexin_WASP-bd-dom.
    [Graphical view ]
    PANTHERi PTHR10555:SF14. PTHR10555:SF14. 1 hit.
    Pfami PF10456. BAR_3_WASP_bdg. 1 hit.
    PF00787. PX. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF027744. Snx9. 1 hit.
    SMARTi SM00312. PX. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    2. "Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1."
      Howard L., Nelson K.K., Maciewicz R.A., Blobel C.P.
      J. Biol. Chem. 274:31693-31699(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ADAM9 AND ADAM15.
    3. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Solution structure of the SH3 domain from mouse sorting nexin-9."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2007) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 1-70.

    Entry informationi

    Entry nameiSNX9_MOUSE
    AccessioniPrimary (citable) accession number: Q91VH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3