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Protein

Histamine N-methyltransferase

Gene

Hnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + histamine = S-adenosyl-L-homocysteine + N(tau)-methylhistamine.PROSITE-ProRule annotation

Kineticsi

  1. KM=5.8 µM for S-adenosyl-L-methionine1 Publication
  2. KM=5.3 µM for histamine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei28 – 281SubstratePROSITE-ProRule annotation
    Binding sitei60 – 601S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei89 – 891S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei94 – 941S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei120 – 1201S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei143 – 1431S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
    Binding sitei284 – 2841SubstratePROSITE-ProRule annotation

    GO - Molecular functioni

    • histamine N-methyltransferase activity Source: GO_Central
    • N-methyltransferase activity Source: MGI

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histamine N-methyltransferase (EC:2.1.1.8)
    Short name:
    HMT
    Gene namesi
    Name:Hnmt
    Synonyms:Hmt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 2

    Organism-specific databases

    MGIiMGI:2153181. Hnmt.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 295295Histamine N-methyltransferasePRO_0000084022Add
    BLAST

    Proteomic databases

    MaxQBiQ91VF2.
    PaxDbiQ91VF2.
    PRIDEiQ91VF2.

    PTM databases

    PhosphoSiteiQ91VF2.

    Expressioni

    Gene expression databases

    BgeeiQ91VF2.
    CleanExiMM_HNMT.
    ExpressionAtlasiQ91VF2. baseline and differential.
    GenevisibleiQ91VF2. MM.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000062747.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91VF2.
    SMRiQ91VF2. Positions 5-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. HNMT family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG39680.
    GeneTreeiENSGT00390000002862.
    HOGENOMiHOG000231790.
    HOVERGENiHBG051914.
    InParanoidiQ91VF2.
    KOiK00546.
    OMAiMDISDCF.
    OrthoDBiEOG7Z69CQ.
    PhylomeDBiQ91VF2.
    TreeFamiTF331080.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR016673. Histamine_N-methyltransferase.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PIRSFiPIRSF016616. HHMT. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51597. SAM_HNMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91VF2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASCMRSLFS DQGRYVESFR RFLNNSTEHQ CMQEFMDKKL PGIIARIGEA
    60 70 80 90 100
    KAEIKILSVG GGAGEVDLQI LSKVQAQYPG ICINNEVVEP SAEQIVKYKE
    110 120 130 140 150
    LVAKTSNMEN IKFSWHKETS SEYQKRMLEE EEEPPKWDFI HMIQMLYYVK
    160 170 180 190 200
    DIPATLKFFH GLLAASAKIL IILVSGTSGW EKLWKKYGSR LPRDDLCQYV
    210 220 230 240 250
    TSSDLAQILD DLGIKYECYD LVSTMDITDC FIDGNENGDL LWDFLTETCN
    260 270 280 290
    FSKTAPLDLK AEIMKDLQEP EFSVKKEGKV LFNNNLSFIV VEANV
    Length:295
    Mass (Da):33,665
    Last modified:December 1, 2001 - v1
    Checksum:i04FA324ED14A51CA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF297037 mRNA. Translation: AAL09305.1.
    AF298150
    , AF297039, AF297040, AF297041, AF297042, AF297043 Genomic DNA. Translation: AAL09306.1.
    AB070523 Genomic DNA. Translation: BAB84320.1.
    AB070524 mRNA. Translation: BAB84318.1.
    AK050129 mRNA. Translation: BAC34081.1.
    AK160131 mRNA. Translation: BAE35648.1.
    BC033928 mRNA. Translation: AAH33928.1.
    CCDSiCCDS15731.1.
    RefSeqiNP_536710.1. NM_080462.2.
    XP_006497734.1. XM_006497671.2.
    UniGeneiMm.33120.

    Genome annotation databases

    EnsembliENSMUST00000051416; ENSMUSP00000062747; ENSMUSG00000026986.
    ENSMUST00000114497; ENSMUSP00000110141; ENSMUSG00000026986.
    ENSMUST00000114498; ENSMUSP00000110142; ENSMUSG00000026986.
    GeneIDi140483.
    KEGGimmu:140483.
    UCSCiuc008iok.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF297037 mRNA. Translation: AAL09305.1.
    AF298150
    , AF297039, AF297040, AF297041, AF297042, AF297043 Genomic DNA. Translation: AAL09306.1.
    AB070523 Genomic DNA. Translation: BAB84320.1.
    AB070524 mRNA. Translation: BAB84318.1.
    AK050129 mRNA. Translation: BAC34081.1.
    AK160131 mRNA. Translation: BAE35648.1.
    BC033928 mRNA. Translation: AAH33928.1.
    CCDSiCCDS15731.1.
    RefSeqiNP_536710.1. NM_080462.2.
    XP_006497734.1. XM_006497671.2.
    UniGeneiMm.33120.

    3D structure databases

    ProteinModelPortaliQ91VF2.
    SMRiQ91VF2. Positions 5-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000062747.

    PTM databases

    PhosphoSiteiQ91VF2.

    Proteomic databases

    MaxQBiQ91VF2.
    PaxDbiQ91VF2.
    PRIDEiQ91VF2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000051416; ENSMUSP00000062747; ENSMUSG00000026986.
    ENSMUST00000114497; ENSMUSP00000110141; ENSMUSG00000026986.
    ENSMUST00000114498; ENSMUSP00000110142; ENSMUSG00000026986.
    GeneIDi140483.
    KEGGimmu:140483.
    UCSCiuc008iok.2. mouse.

    Organism-specific databases

    CTDi3176.
    MGIiMGI:2153181. Hnmt.

    Phylogenomic databases

    eggNOGiNOG39680.
    GeneTreeiENSGT00390000002862.
    HOGENOMiHOG000231790.
    HOVERGENiHBG051914.
    InParanoidiQ91VF2.
    KOiK00546.
    OMAiMDISDCF.
    OrthoDBiEOG7Z69CQ.
    PhylomeDBiQ91VF2.
    TreeFamiTF331080.

    Miscellaneous databases

    NextBioi369786.
    PROiQ91VF2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ91VF2.
    CleanExiMM_HNMT.
    ExpressionAtlasiQ91VF2. baseline and differential.
    GenevisibleiQ91VF2. MM.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR016673. Histamine_N-methyltransferase.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PIRSFiPIRSF016616. HHMT. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51597. SAM_HNMT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Mouse histamine N-methyltransferase: cDNA cloning, expression, gene cloning and chromosomal localization."
      Wang L., Yan L., McGuire C., Kozak C.A., Wang M., Kim U.J., Siciliano M., Weinshilboum R.M.
      Inflamm. Res. 50:300-308(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: BALB/c.
      Tissue: Liver.
    2. "Genomic structure of the rat and mouse histamine N-methyltransferase gene."
      Kitanaka N., Kitanaka J., Oue T., Tada Y., Tanaka T., Takemura M.
      Jpn. J. Pharmacol. 88:85-92(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv and ddY.
      Tissue: Liver.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum and Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiHNMT_MOUSE
    AccessioniPrimary (citable) accession number: Q91VF2
    Secondary accession number(s): Q3TVH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: December 1, 2001
    Last modified: June 24, 2015
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.